Transmissible gastroenteritis virus (TGEV) infection causes acute enteritis in swine of all ages, and especially in suckling piglets. Small intestinal inflammation is considered a central event in the pathogenesis of TGEV infections, and nuclear factor-kappa B (NF-κB) is a key transcription factor in the inflammatory response. However, it is unclear whether NF-κB is crucial for inducing inflammation during a TGEV infection. Our results show that NF-κB was activated in swine testicular (ST) cells and intestinal...

Two new alkaloids, phranisines A–B (1–2), along with two known compounds, N-p-Coumaroyl serotonin (3) and N-p-coumaroyl-tryptamine (4), were isolated from the roots of Phragmites australis. The structures of 1–4 were established on the basis of extensive spectroscopic. The absolute configuration of compounds 1–2 were identified through quantum-chemical electronic circular dichroism (ECD) calculation compared with their experimental CD. All the isolated compounds were tested for their cytotoxic activities against HeLa and MCF-7 human cancer cell lines,...

Diabetic kidney diseases (DKD) were the leading cause of End-stage renal diseases worldwide. Albuminuria was a target for treatment in DKD and decreasing albuminuria was particularly important for improving its prognosis. However, there is still a lack of specific treatment for DKD. We conducted a prospective, crossover, open-label study to investigate the effect of amiloride in patients with DKD. Safety and efficacy were assessed by monitoring urine protein creatinine ratio(uPCR), urinary albumin creatinine ratio (uACR),...

Chemotherapy is currently the main treatment for unresectable or advanced postoperative gastric cancers. However, its efficacy is negatively affected by the occurrence of chemoresistance, which severely affects patient prognosis. Recently, dysregulation in autophagy has been suggested as a potential mechanism for chemoresistence, and long noncoding RNA (lncRNA) also shows its regulatory role in cancer drug resistance. Using RNA sequencing, we found that lncRNA EIF3J-DT was highly expressed in drug-resistant gastric cancer cells. In-vitro and in-vivo...

The mitochondrial genome of the tachinid fly Peleteria iavana (Wiedemann, 1819), which belongs to the family Tachinidae, was obtained for the first time using high-throughput sequencing techniques. The complete mitochondrial genome is 15,697 bp in size and consists of 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNAs and a non-coding control region. The nucleotide composition biases A and T, the overall A + T percentage is up to 78.9% of the entire...

The fitness of multidrug-resistant tuberculosis (MDR-TB) is thought to be an important determinant of a strain’s ability to be transmitted. Studies in the laboratory have demonstrated that MDR-TB strains have reduced fitness but the relative transmissibility of MDR-TB versus drug-susceptible (DS) TB strains in human populations remains unresolved. We used data on genomic clustering from our previous molecular epidemiological study in Songjiang (2011-2020) and Wusheng (2009-2020), China, to compare the relative transmissibility of MDR-TB versus...

The fitness of multidrug-resistant tuberculosis (MDR-TB) is thought to be an important determinant of a strain’s ability to be transmitted and cause outbreaks. Studies in the laboratory have demonstrated that MDR-TB strains have reduced fitness but the relative transmissibility of MDR-TB versus drug-susceptible (DS) TB strains in human populations remains unresolved. We used data on genomic clustering from our previous molecular epidemiological study in Songjiang (2011-2020) and Wusheng (2009-2020), China, to compare the relative transmissibility...

In the fusion design process of the system, ill-conditioned features, that are not explicit in previous generation systems, may exhibit to negate the operation of the new-generation system. This type of ill-conditioned feature is termed as the recessive ill-conditioned feature (R-ICF) of the system. It is believed that R-ICF in the fusion design process is attributed to the complex system recessive inheritance mechanism (C-RIM), which is analogical to organism recessive inheritance. In-depth study of the...

Multiple myeloma (MM) remains an incurable disease despite advances in treatment options. Recently, selinexor has shown promising efficacy for relapsed/refractory multiple myeloma (RRMM), whereas its optimal timing and drug combination remain unclear. In order to assess the various regimens that incorporate selinexor, a systematic review and meta-analysis was conducted. Clinical trials and real-world studies involving MM patients treated with selinexor were included. Pooled risk ratio (RR) was calculated to compare the rates, along with a...

The oxidation of sulfur dioxide (SO2) to sulfate in the atmosphere is an important concern in regional air quality, global climate change, and human health. While gas-phase and liquid-phase oxidation of SO2 are widely regarded as important sources of sulfate, the contribution of the heterogeneous oxidation process on particle surfaces is controversial. Recently, this heterogeneous chemistry has been considered to be an important mechanism that is missing in current models to explain sulfate concentrations observed...

We selected 43 grassland sites along a 1,500-km latitudinal gradient (c. 27°N to 39°N) and a 2,698-m elevational gradient (1,886 m to 4,584 m) on the Qinghai-Tibetan Plateau. We calculated the overall herbivory percentage (ranging from 0% to 100%) for each plant species by first multiplying the number of leaves in each damage class by the median removal value for that class (i.e., 0%, 2.5%, 7.5%, 15.0%, 35.0%, or 75.0%). Obtained values were then summed...

Additional file 3: Fig. S3. Radiomics features selected by LASSO Cox for OS prediction.

Abstract Background The flipped classroom (FC) method is becoming increasingly popular in China's nursing education. It is an important breakthrough improvement in the quality of learning in nursing education reforms. Purpose This study aimed to determine the effects of blended task-oriented flipped classroom (TFC) on nursing students undertaking the Fundamentals of Nursing course. Methods A pre-and post-test quasi-experimental design was adopted. This study was conducted in the Autumn semester, 2021 academic year in a Chinese...

Additional file 2: Table S2. Surgical evaluation before and after neoadjuvant therapy.

Additional file 1: Supplementary figure 1. Landscape of the expression levels and genetic alterations of 5mC/m6A regulators across 33 cancer types. (A) Gene expression profiling of 5mC/m6A regulators across pan-cancer. For a given 5mC/m6A regulator in each cancer type, the median expression value is shown. (B, C) Spearman correlations of CNVs with transcript levels of 5mC/m6A regulators in pan-cancer, reflecting the gene expression significantly affected by CNVs. (D, E) Pie chart for heterozygous and homozygous...

Abstract Background Immunotherapy has revolutionized cancer treatment, but most patients are refractory to immunotherapy or acquire resistance, with the underlying mechanisms remaining to be explored. Methods We characterized the transcriptomes of ~92,000 single cells from 3 pre-treatment and 12 post-treatment patients with non-small cell lung cancer (NSCLC) who received neoadjuvant PD-1 blockade combined with chemotherapy. The 12 post-treatment samples were categorized into two groups based on pathologic response: major pathologic response (MPR; n = 4)...

Abstract Background Severe neonatal thrombocytopenia is a rare disease with multiple etiologies. Severe thrombocytopenia with bleeding is life-threatening and has attracted significant attention from clinicians. However, only a few studies have focused on the association between severe thrombocytopenia and bleeding. Thus, this study aimed to describe the neonates’ postnatal age at which severe thrombocytopenia was first recognized, clinical characteristics, bleeding patterns, and outcomes and to evaluate the association between minimum platelet count and bleeding. Methods...

Additional file 1.

Additional file 4: Fig. S4. OS prediction Nomogram and calibration curves of combined model.

Additional file 2: Fig. S2. PFS prediction Nomogram and calibration curve of combined model.

Additional file 1: Fig. S1. Characteristics of human peripheral blood iMos (top row) and pMos (bottom row): iMos and pMos were stained with antibodies of FITC-CD14, PE-CD16, PerCP/Cy5.5-CX3CR1 and APC-CCR2.

Additional file 3: Figure S1. (a) Treatment response for each patient in different treatment cycles (b) A significant correlation was identified between radiological response and pathological response. (c) The difference in OS between the adjuvant immunotherapy maintenance group and the non-maintenance group. (d) The difference in PFS between the adjuvant immunotherapy maintenance group and the non-maintenance group. (e) The difference in OS between the PD-L1 positive group and the negative group. (f) The difference in...

Additional file 1: Supplementary data 1. No significant differences in laboratory parameters between serous and mucinous BEOT patients.

Additional file 2: Supplementary data 2. Comparison of AUC of single and combined use of CA125, NLR and PLR.

Additional file 1. The following are available as supplementary data in the Additional files: Table S1 Individual participant data for demographics and baseline characteristics. Table S2 Measurements of CT-derived variables of necrosis at an individual level. Table S3 Intraoperative data. Table S4 Efficacy endpoints at an individual level. Table S5 Safety endpoints at an individual level.