872 Works

The response surface optimization of β-mannanase produced by Weissella cibaria F1 and its potential in juice clarification

Hairui Ji, Huiying Cao, Li Zhao, Ruiying Na, Wenxiang Ping, Jingping Ge & Dan Zhao
A β-mannanase-producing lactic acid bacteria (LAB) was identified as Weissella cibaria F1 according to physiological and biochemical properties, morphological observations, partial sequence of 16S rRNA gene and API 50 CHL test. In order to improve the yield of β-mannanase, the response surface methodology (RSM) was originally used to optimize the fermentation conditions. The optimization results showed that when the konjac powder, glucose, and initial pH were 9.46 g/L, 14.47 g/L and 5.67, respectively, the β-mannanase...

A Gypsy element contributes to the nuclear retention and transcriptional regulation of the resident lncRNA in locusts

Xia Zhang, Ya′Nan Zhu, Bing Chen & Le Kang
The majority of long noncoding RNAs (lncRNAs) contain transposable elements (TEs). PAHAL, a nuclear-retained lncRNA that is inserted by a Gypsy retrotransposon, has been shown to be a vital regulator of phenylalanine hydroxylase (PAH) gene expression that controls dopamine biosynthesis and behavioural aggregation in the migratory locust. However, the role of the Gypsy retrotransposon in the transcriptional regulation of PAHAL remains unknown. Here, we identified a Gypsy retrotransposon (named Gypsy element) as an inverted long...

Integrated bioinformatics and experiments reveal the roles and driving forces for HSF1 in colorectal cancer

Xiaomin Ren, Liyuan Zhang, Xiaolin Ma, Jiaqiu Li & Zhong Lu
Heat shock factor 1 (HSF1) has watershed significance in different tumors. However, the roles and driving forces for HSF1 in colorectal cancer (CRC) are poorly understood. Our study integrally analyzed the roles and driving forces for HSF1 in CRC by bioinformatics and experiments. The expression and prognostic characteristics of HSF1 were analyzed via UALCAN, GEPIA2, TISIDB, Prognoscan and HPA databases. Then, we analyzed the correlation between HSF1 expression and immune features via TIMER2 database. Subsequently,...

ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation

Jilong Zhou, Hainan He, Jing-Jing Zhang, Xin Liu, Wang Yao, Chengyu Li, Tian Xu, Shu-Yuan Yin, Dan-Ya Wu, Cheng-Li Dou, Qiao Li, Jiani Xiang, Wen-Jing Xiong, Li-Yan Wang, Jun-Ming Tang, Zhouyiyuan Xue, Xia Zhang & Yi-Liang Miao
Macroautophagy/autophagy is a conserved cellular mechanism to degrade unneeded cytoplasmic proteins and organelles to recycle their components, and it is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Whereas autophagy is essential for early development of embryos, no information exists regarding its functions during the transition from naive-to-primed pluripotency. Here, by using an in vitro transition model of ESCs to epiblast-like cells (EpiLCs), we find that dynamic changes in ATG7-dependent autophagy are...

A tandem-repeat dimeric RBD protein-based COVID-19 vaccine ZF2001 protects mice and nonhuman primates

Yaling An, Shihua Li, Xiyue Jin, Jian-bao Han, Kun Xu, Senyu Xu, Yuxuan Han, Chuanyu Liu, Tianyi Zheng, Mei Liu, Mi Yang, Tian-zhang Song, Baoying Huang, Li Zhao, Wen Wang, A Ruhan, Yingjie Cheng, Changwei Wu, Enqi Huang, Shilong Yang, Gary Wong, Yuhai Bi, Changwen Ke, Wenjie Tan, Jinghua Yan … & George F. Gao
Safe, efficacious and deployable vaccines are urgently needed to control COVID-19 in the large-scale vaccination campaigns. We report here the preclinical studies of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain (RBD) protein with alum-based adjuvant. We assessed vaccine immunogenicity and efficacy in both mice and non-human primates (NHPs). ZF2001 induced high levels of RBD-binding and SARS-CoV-2 neutralizing antibody in both mice and non-human primates, and elicited balanced TH1/TH2...

Three new flavonoid glycosides from the aerial parts of Allium sativum L. and their anti-platelet aggregation assessment

Bin Hu, Haibo Hu, Chuan Pu, Zheng Wei, Qiuhong Wang & Haixue Kuang
Three new flavonoid glycosides, Dasuanxinoside F–H (1–3), were isolated from the aerial parts of Allium sativum, together with eight known compounds which were firstly reported in this plant, including three flavonoid glycosides (4–6) and five phenylethanoid glycosides (7–11). Their structures were identified by UV-vis, IR, 1D and 2D NMR spectra, as well as HR-ESI-MS analyses. The inhibitory effect of the isolated compounds on platelet aggregation induced by adenosine diphosphate (ADP) was evaluated in vitro. The...

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity

Guokai Yan, Xiuzhi Li, Zilong Zheng, Weihua Gao, Changqing Chen, Xinkai Wang, Zhongyi Cheng, Jie Yu, Geng Zou, Muhammad Zahid Farooq, Xiaoyan Zhu, Weiyun Zhu, Qing Zhong & Xianghua Yan
Amino acids play crucial roles in the MTOR (mechanistic target of rapamycin kinase) complex 1 (MTORC1) pathway. However, the underlying mechanisms are not fully understood. Here, we establish a cell-free system to mimic the activation of MTORC1, by which we identify CANX (calnexin) as an essential regulator for leucine-stimulated MTORC1 pathway. CANX translocates to lysosomes after leucine deprivation, and its loss of function renders either the MTORC1 activity or the lysosomal translocation of MTOR insensitive...

Subspace Estimation with Automatic Dimension and Variable Selection in Sufficient Dimension Reduction

Jing Zeng, Qing Mai & Xin Zhang
Sufficient dimension reduction (SDR) methods target finding lower-dimensional representations of a multivariate predictor to preserve all the information about the conditional distribution of the response given the predictor. The reduction is commonly achieved by projecting the predictor onto a low-dimensional subspace. The smallest such subspace is known as the Central Subspace (CS) and is the key parameter of interest for most SDR methods. In this article, we propose a unified and flexible framework for estimating...

Hybodontiform shark remains (Chondrichthyes, Elasmobranchii) from the Lower Triassic of Yunnan Province, China, with comments on hybodontiform diversity across the PTB

Wen Wen, JüRgen Kriwet, Qiyue Zhang, Michael J. Benton, Christopher J. Duffin, Jingyuan Huang, Changyong Zhou, Shixue Hu & Zhixin Ma
Hybodontiform shark teeth, cephalic and fin spines as well as placoid scales are reported here for the first time from the continental, Lower Triassic Dongchuan Formation near the village of Zhaojia (Huize County, Yunnan Province) in south-west China. The isolated teeth are assigned to three unidentified hybodontiforms but also to a new species of Parvodus, P. huizodus sp. nov., the oldest record of this small-toothed lonchidiid shark genus, extending its range well down into the...

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer

Di Xu, Wen-Juan Tang, Yi-Zhi Zhu, Zhen Liu, Kai Yang, Ming-Xing Liang, Xiu Chen, Yang Wu, Jin-Hai Tang & Wei Zhang
To investigate the mechanism through which hyperthermia promotes exosome secretion and drug sensitivity in adriamycin-resistant breast cancer. We first evaluated the effect of hyperthermia on adriamycin-resistant breast cancer viability and used transmission electron microscopy, nanoparticle tracking analysis, and a bicinchoninic acid kit to validate the effect of hyperthermia on exosome secretion. The effective targeting molecules and pathways changed by hyperthermia were explored by RNA microarray and verified in vitro. The adriamycin-resistant MCF-7/ADR cells co-incubated with...

Alleviative effects of foraging exercise on depressive-like behaviors in chronic mild stress-induced ischemic rat model

Xi Tao, Siyuan Wu, Wenjing Tang, Lu Li, Lijun Huang, Danheng Mo, Chujuan Liu, Tao Song, Shuling Wang, Jia Wang & Juan He
Poststroke depression (PSD) is a common complication that seriously affects the functional recovery and prognosis of an individual. As some patients with PSD fail to respond to drug therapy, it is urgent to find a viable alternative treatment. An active exercise program known as foraging exercise (FE), using food as bait, was designed. First, focal ischemia and chronic unpredictable mild stress (CUMS) were used to establish a PSD model in rats. FE was then performed...

Predictors of HbA1c reduction and hypoglycemia in type 2 diabetes mellitus individuals switching from premixed to basal insulin: an exploratory analysis of optimization study

Wenying Yang, Juan Du, Minlu Zhang, Jing Hou, Xia Zhang & Nan Cui
To identify population with type 2 diabetes mellitus (T2DM) who are more likely to benefit from switching to basal insulin (BI) treatment from premixed insulin. This secondary analysis included data from a previously published study (Optimization: NCT00693771) which was a single-arm, multicenter, 16 weeks, phase IV study. The analysis included participants with T2DM inadequately controlled with premixed insulin plus oral hypoglycemic drugs (OADs) who switched to BI plus OADs. Among the 297 participants included for...

Identification and potential mechanism of different components from the aerial part of Bupleurum chinense DC. for epileptic treatment

Xiaomao Li, Yan Liu, Siyi Wang, Wei Guan, Juan Pan, Haixue Kuang & Bingyou Yang
The present study aimed to assess the effects of epileptic treatment of the aerial part of Bupleurum chinense DC. in kainic acid (KA)-induced epilepsy rats and LPS-induced BV2 cells, as well as to identify the active chemical constituents. The in vivo and vitro results showed that 20% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-20) and 70% ethanol elution fractions of the aerial part of B. chinense DC. (BCE-70) could improve...

Gapless genome assembly of East Asian finless porpoise, Neophocaena asiaeorientalis sunameri

Denghua Yin, Chunhai Chen, Danqing Lin, Jialun Zhang, Congping Ying, Yan Liu, Wang Liu, Zhichen Cao, Chenxi Zhao, Chenhe Wang, Liping Liang, Pao Xu, Jianbo Jian & Kai Liu
There are the genome sequence, gene set, coding sequence and protein sequence of Neophocaena asiaeorientalis sunameri.

A foetus with a mass in the oral cavity: a rare case of oral eruption cyst

Qianqian Gao, Yu Wang, Hanmin Liu & Jiao Chen
A foetus with a mass in the oral cavity: a rare case of oral eruption cyst

A foetus with a mass in the oral cavity: a rare case of oral eruption cyst

Qianqian Gao, Yu Wang, Hanmin Liu & Jiao Chen
A foetus with a mass in the oral cavity: a rare case of oral eruption cyst

Associations of RPEL1 and miR-1307 gene polymorphisms with disease susceptibility, glucocorticoid efficacy, anxiety, depression, and health-related quality of life in Chinese systemic lupus erythematosus patients

Zi-Ye Yan, Wan-Qin Hu, Qi-Qun Zong, Guang-Hui Yu, Chun-Xia Zhai, Lin-Lin Wang, Yu-Hua Wang, Ting-Yu Zhang, Zhen Li, Ying Teng, Jing Cai, Yang-Fan Chen, Mu Li, Zhou-Zhou Xu, Fa-Ming Pan, Hai-Feng Pan, Hong Su & Yan-Feng Zou
ObjectiveOur present study intended to examine the associations of RPEL1 and miR-1307 gene polymorphisms (rs4917385 and rs7911488) with susceptibility, glucocorticoids (GCs) efficacy, anxiety, depression, and health-related quality of life (HRQoL) in Chinese systemic lupus erythematosus (SLE) patients.MethodsInitially, 1000 participants (500 SLE cases and 500 controls) were recruited for the case–control study. Then, 429 cases who received GCs were followed through 12 weeks to explore GCs efficacy, depression, anxiety, and HRQoL. We selected the iMLDR technique...

Associations of RPEL1 and miR-1307 gene polymorphisms with disease susceptibility, glucocorticoid efficacy, anxiety, depression, and health-related quality of life in Chinese systemic lupus erythematosus patients

Zi-Ye Yan, Wan-Qin Hu, Qi-Qun Zong, Guang-Hui Yu, Chun-Xia Zhai, Lin-Lin Wang, Yu-Hua Wang, Ting-Yu Zhang, Zhen Li, Ying Teng, Jing Cai, Yang-Fan Chen, Mu Li, Zhou-Zhou Xu, Fa-Ming Pan, Hai-Feng Pan, Hong Su & Yan-Feng Zou
ObjectiveOur present study intended to examine the associations of RPEL1 and miR-1307 gene polymorphisms (rs4917385 and rs7911488) with susceptibility, glucocorticoids (GCs) efficacy, anxiety, depression, and health-related quality of life (HRQoL) in Chinese systemic lupus erythematosus (SLE) patients.MethodsInitially, 1000 participants (500 SLE cases and 500 controls) were recruited for the case–control study. Then, 429 cases who received GCs were followed through 12 weeks to explore GCs efficacy, depression, anxiety, and HRQoL. We selected the iMLDR technique...

Additional file 5 of Genome-wide identification, new classification, expression analysis and screening of drought & heat resistance related candidates in the RING zinc finger gene family of bread wheat (Triticum aestivum L.)

Yongliang Li, Pai Qin, Aolong Sun, Wenjun Xiao, Fenglin Chen, Yang He, Keyao Yu, You Li, Meng Zhang & Xinhong Guo
Additional file 5: Table S2. One-to-one orthologous relationships among T.aestivum and T.aestivum, A.thaliana, A. tauschii, B. distachyon, H. vulgare, rice, S.italica and Zea mays, respectively.

Additional file 6 of Genome-wide identification, new classification, expression analysis and screening of drought & heat resistance related candidates in the RING zinc finger gene family of bread wheat (Triticum aestivum L.)

Yongliang Li, Pai Qin, Aolong Sun, Wenjun Xiao, Fenglin Chen, Yang He, Keyao Yu, You Li, Meng Zhang & Xinhong Guo
Additional file 6: Table S3. The information of cis-acting elements on the promoters of T. aestivum RING-zf genes.

Additional file 6 of Genome-wide identification, new classification, expression analysis and screening of drought & heat resistance related candidates in the RING zinc finger gene family of bread wheat (Triticum aestivum L.)

Yongliang Li, Pai Qin, Aolong Sun, Wenjun Xiao, Fenglin Chen, Yang He, Keyao Yu, You Li, Meng Zhang & Xinhong Guo
Additional file 6: Table S3. The information of cis-acting elements on the promoters of T. aestivum RING-zf genes.

Additional file 2 of Estimating disability-adjusted life years for breast cancer and the impact of screening in female populations in China, 2015–2030: an exploratory prevalence-based analysis applying local weights

Xin-Xin Yan, Juan Zhu, Yan-Jie Li, Meng-Di Cao, Xin Wang, Hong Wang, Cheng-Cheng Liu, Jing Wang, Yang Li & Ju-Fang Shi
Additional file 2: Clinical stage-specific disability weights and distribution of breast cancer in females in China.

Additional file 4 of Estimating disability-adjusted life years for breast cancer and the impact of screening in female populations in China, 2015–2030: an exploratory prevalence-based analysis applying local weights

Xin-Xin Yan, Juan Zhu, Yan-Jie Li, Meng-Di Cao, Xin Wang, Hong Wang, Cheng-Cheng Liu, Jing Wang, Yang Li & Ju-Fang Shi
Additional file 4: Age-specific number of females and standardized life expectancy

Additional file 1 of Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonist

Yu Bai, Xin Zhang, Jie Zheng, Ziyi Liu, Zhenfan Yang & Xiaolin Zhang
Supplementary Material 1

Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonist

Yu Bai, Xin Zhang, Jie Zheng, Ziyi Liu, Zhenfan Yang & Xiaolin Zhang
Abstract Background Adenosine is a potent immunosuppressant whose levels in the tumor microenvironment (TME) are often much higher than those in normal tissues. Binding of adenosine to its receptor A2aR activates a cascade of genes and leads to immunosuppression. In addition, immune checkpoint blockage markedly increases A2aR expression in T cells, which could dampen their anti-tumor response. Several A2aR antagonists are under clinical development, but with limited clinical benefit reported so far. These A2aR antagonists...

Registration Year

  • 2022
    872

Resource Types

  • Text
    368
  • Dataset
    198
  • Collection
    197
  • Image
    91
  • Audiovisual
    10
  • Other
    8

Affiliations

  • Inner Mongolia Agricultural University
    872
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    607
  • Shanghai Jiao Tong University
    603
  • Chinese Academy of Sciences
    538
  • Jilin University
    522
  • Peking University
    516
  • Capital Medical University
    501
  • Southern Medical University
    489
  • Zhejiang University
    451
  • Shandong University
    444