Data from: Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studiesCédric Mariac, Nora Scarcelli, Juliette Pouzadou, Adeline Barnaud, Claire Billot, Adama Faye, Vincent Maillol, Sylvain Santoni, Yves Vigouroux, Thomas L. P. Couvreur, Ayite Kougbeadjo, François Sabot & Guillaume Martin
Biodiversity, phylogeography and population genetic studies will be revolutionized by access to large datasets thanks to next generation sequencing methods. In this study, we develop an easy and cost-effective protocol for in-solution enrichment hybridization capture of complete chloroplast genomes applicable at deep multiplexed levels. The protocol uses cheap in-house species-specific probes developed via long range PCR of the entire chloroplast. Barcoded libraries are constructed and in-solution enrichment of the chloroplasts is done using the probes....
Data from: Phylogeography in continuous space: coupling species distribution models and circuit theory to assess the effect of contiguous migration at different climatic periods on genetic differentiation in Busseola fusca (Lepidoptera: Noctuidae)Stéphane Dupas, Bruno Le Rü, Antoine Branca, Nathalie Faure, Guillaume Gigot, Pascal Campagne, Michel Sezonlin, Rose Ndemah, Paul-André Calatayud, Jean-François Silvain, G. Ong'amo, B. Le Ru, P.-A. Calatayud & J.-F. Silvain
Current population genetic models fail to cope with genetic differentiation for species with large, contiguous and heterogeneous distribution. We show that in such a case, genetic differentiation can be predicted at equilibrium by circuit theory, where conductance corresponds to abundance in species distribution models (SDM). Circuit-SDM approach was used for the phylogeographic study of the lepidopteran cereal stemborer Busseola fusca Füller (Noctuidae) across sub-Saharan Africa. Species abundance was surveyed across its distribution range. SDM models...
Data from: Habitat segregation and ecological character displacement in cryptic African malaria mosquitoesBilly Tene Fossog, Diego Ayala, Pelayo Acevedo, Pierre Kengne, Ignacio Ngomo Abeso Mebuy, Boris Makanga, Julie Magnus, Parfait Awono-Ambene, Flobert Njiokou, Marco Pombi, Christophe Antonio-Nkondjio, Christophe Paupy, Nora J. Besansky & Carlo Costantini
Understanding how divergent selection generates adaptive phenotypic and population diversification provides a mechanistic explanation of speciation in recently separated species pairs. Toward this goal, we sought ecological gradients of divergence between the cryptic malaria vectors Anopheles coluzzii and An. gambiae, and then looked for a physiological trait that may underlie such divergence. Using a large set of occurrence records and eco-geographical information, we built a distribution model to predict the predominance of the two species...
Data from: Diversification of an emerging pathogen in a biodiversity hotspot: Leptospira in endemic small mammals of MadagascarMuriel Dietrich, David A. Wilkinson, Voahangy Soarimalala, Steven M. Goodman, Koussay Dellagi & Pablo Tortosa
Biodiversity hotspots and associated endemism are ideal systems for the study of parasite diversity within host communities. Here, we investigated the ecological and evolutionary forces acting on the diversification of an emerging bacterial pathogen, Leptospira spp., in communities of endemic Malagasy small mammals. We determined the infection rate with pathogenic Leptospira in 23 species of sympatric rodents (subfamily Nesomyinae) and tenrecids (family Tenrecidae) at two eastern humid forest localities. A multilocus genotyping analysis allowed the...
Simian immunodeficiency viruses (SIVs) have been discovered in over 45 primate species; however, the pathogenic potential of most SIV strains remains unknown due to difficulties inherent in observing wild populations. Because those SIV infections that are pathogenic have been shown to induce changes in the host's gut microbiome, monitoring the microbiota present in faecal samples can provide a noninvasive means for studying the effects of SIV infection on the health of wild-living primates. Here, we...
Data from: Nurse-based restoration of degraded tropical forests with tussock grasses: experimental support from the Andean cloud forestFabien Anthelme, Lorena Gómez-Aparicio & Rommel Montúfar
1. The degradation of the Andean cloud forest raises strong biological conservation issues and threatens the sustainability of a crucial water resource. The idea that nurse-based restoration can accelerate the recovery of these forests is underexplored, despite its promise as a restoration technique. Recent conceptual models predict that facilitation among plants may be an important mechanism, but there is a lack of strong empirical support. We gathered experimental data to test this prediction and explore...
Institut de Recherche pour le Développement6
Institut de Recherche pour le Développement3
Université de Yaoundé I2
Institut de Recherche pour le Développement2
University of Pennsylvania1
The University of Texas at Austin1
Field Museum of Natural History1
University of Notre Dame1
Institute for Game and Wildlife Research1
Centre International de Recherches Médicales de Franceville1