Data from: Thermal limits in native and alien freshwater peracarid Crustacea: the role of habitat use and oxygen limitationWilco C.E.P. Verberk, Rob S.E.W. Leuven, Gerard Van Der Velde & Friederike Gabel
1. In order to predict which species can successfully cope with global warming and how other environmental stressors modulate their vulnerability to climate related environmental factors, an understanding of the ecophysiology underpinning thermal limits is essential for both conservation biology a nd invasion biology. 2. Heat tolerance and the extent to which heat tolerance differed with oxygen availability were examined for four native and four alien freshwater peracarid crustacean species, with differences in habitat use...
Data from: Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosaLin Li, Xiaodong Jiao, Ilaria D’Atri, Fumihito Ono, Ralph Nelson, Chi-Chao Chan, Naoki Nakaya, Zhiwei Ma, Yan Ma, Xiaoying Cai, Longhua Zhang, Siying Lin, Abdul Hameed, Barry A. Chioza, Holly Hardy, Gavin Arno, Sarah Hull, Muhammad Imran Khan, James Fasham, V. Gaurav Harlalka, Michel Michaelides, Anthony T. Moore, Zeynep Hande Coban Akdemir, Shalini Jhangiani, James R. Lupski … & Frans P. M. Cremers
We identified a homozygous missense alteration (c.75C>A, p.D25E) in CLCC1, encoding a presumptive intracellular chloride channel highly expressed in the retina, associated with autosomal recessive retinitis pigmentosa (arRP) in eight consanguineous families of Pakistani descent. The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. TALEN KO in zebrafish was lethal 11 days post...
Data from: Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina FasoAissata Barry, Marije C. Behet, Issa Nébié, Kjerstin Lanke, Lynn Grignard, Alphonse Ouedraogo, Issiaka Soulama, Chris Drakeley, Robert Sauerwein, Judith M. Bolscher, Koen J. Dechering, Teun Bousema, Alfred B. Tiono & Bronner P. Gonçalves
Background: Individuals living in malaria-endemic regions develop naturally acquired immunity against severe malarial disease, but it is unclear whether immunity that affects the establishment of infections develops following continuous natural exposure. Methods: We cleared schoolchildren in Burkina Faso of possible sub-patent infections and examined them weekly for incident infections by PCR. Plasma samples collected at enrolment were used to quantify antibodies to the pre-eryhrocytic-stage antigens circumsporozoite protein (CSP) and liver stage antigen. Sporozoite gliding inhibition...
Objective: An observational cross-sectional study was conducted in a national facioscapulohumeral muscular dystrophy (FSHD) expertise center to estimate the penetrance of FSHD1 and to evaluate phenotype–genotype correlations. Methods: Ten FSHD1 probands carrying 4–9 D4Z4 unit alleles and 140 relatives were examined. All 150 participants were genetically characterized, including D4Z4 methylation levels in the mutation carriers. Mutation carriers were classified as (1) symptomatic: with symptoms of muscle weakness on history and muscle FSHD signs on examination;...
Data from: Costs and benefits of admixture between foreign genotypes and local populations in the fieldJun Shi, Jasmin Joshi, Katja Tielborger, Koen J. F. Verhoeven & Mirka Macel
Admixture is the hybridization between populations within one species. It can increase plant fitness and population viability by alleviating inbreeding depression and increasing genetic diversity. However, populations are often adapted to their local environments and admixture with distant populations could break down local adaptation by diluting the locally adapted genomes. Thus, admixed genotypes might be selected against and be outcompeted by locally adapted genotypes in the local environments. To investigate the costs and benefits of...
Intraspecific hybridization between diverged populations can enhance fitness via various genetic mechanisms. The benefits of such admixture have been proposed to be particularly relevant in biological invasions, when invasive populations originating from different source populations are found sympatrically. However, it remains poorly understood if admixture is an important contributor to plant invasive success and how admixture effects compare between invasive and native ranges. Here, we used experimental crosses in Lythrum salicaria, a species with well-established...
Data from: Modeling the impact of Plasmodium falciparum sexual stage immunity on the composition and dynamics of the human infectious reservoir for malaria in natural settingsAndré Lin Ouédraogo, Philip A. Eckhoff, Adrian J. F. Luty, Will Roeffen, Robert W. Sauerwein, Teun Bousema & Edward A. Wenger
Malaria transmission remains high in Sub-Saharan Africa despite large-scale implementation of malaria control interventions. A comprehensive understanding of the transmissibility of infections to mosquitoes may guide the design of more effective transmission reducing strategies. The impact of P. falciparum sexual stage immunity on the infectious reservoir for malaria has never been studied in natural settings. Repeated measurements were carried out at start-wet, peak-wet and dry season, and provided data on antibody responses against gametocyte/gamete antigens...
Data from: A randomized feasibility trial comparing four antimalarial drug regimens to induce Plasmodium falciparum gametocytemia in the controlled human malaria infection modelIsaie J. Reuling, Lisanne A Van De Schans, Luc E Coffeng, Kjerstin Lanke, Lisette Meerstein-Kessel, Wouter Graumans, Geert-Jan Van Gemert, Karina Teelen, Rianne Siebelink-Stoter, Marga Van De Vegte-Bolmer, Quirijn De Mast, André J Van Der Ven, Karen Ivinson, Cornelus C Hermsen, Sake De Vlas, John Bradley, Katharine A Collins, Christian F Ockenhouse, James McCarthy, Robert W. Sauerwein & Teun Bousema
Background: Malaria elimination strategies require a thorough understanding of parasite transmission from human to mosquito. A clinical model to induce gametocytes to understand their dynamics and evaluate transmission-blocking interventions (TBI) is currently unavailable. Here, we explore the use of the well-established Controlled Human Malaria Infection model (CHMI) to induce gametocyte carriage with different antimalarial drug regimens. Methods: In a single centre, open-label randomised trial, healthy malaria-naive participants (aged 18–35 years) were infected with Plasmodium falciparum...
Data from: 18F-fluorodeoxyglucose positron-emission tomography (FDG-PET)-Radiomics of metastatic lymph nodes and primary tumor in non-small cell lung cancer (NSCLC) – A prospective externally validated studySara Carvalho, Ralph T. H. Leijienaar, Esther G. C. Troost, Janna E. Van Timmeren, Cary Oberije, Wouter Van Elmpt, Lioe-Fee De Geus-Oei, Johan Bussink, Philippe Lambin & Ralph T. H. Leijenaar
Background: Lymph node stage prior to treatment is strongly related to disease progression and poor prognosis in non-small cell lung cancer (NSCLC). However, few studies have investigated metabolic imaging features derived from pre-radiotherapy 18F-fluorodeoxyglucose (FDG) positron-emission tomography (PET) of metastatic hilar/mediastinal lymph nodes (LNs). We hypothesized that these would provide complementary prognostic information to FDG-PET descriptors to only the primary tumor (tumor). Methods: Two independent cohorts of 262 and 50 node-positive NSCLC patients were used...
Objective: To describe and classify the neurological trajectories in patients with mild neurological forms of ataxia telangiectasia (A-T) from the Dutch A-T cohort, combined with patients reported in the literature. Methods: Clinical, genetic and laboratory data of 14 A-T patients with mild neurological phenotypes from the Dutch cohort were analyzed and combined with corresponding data from the literature. A mild neurological phenotype was defined by a later onset, non-ataxia presenting or dominant feature, or slower...
Radboud University Nijmegen10
London School of Hygiene & Tropical Medicine2
Nederlands Instituut voor Ecologie2
University of Tübingen2
Leiden University Medical Center2
QIMR Berghofer Medical Research Institute1
University of Twente1
COMSATS Institute of Information Technology1
University of Münster1
University of the Punjab1