Abstract Background For mechanically ventilated critically ill COVID-19 patients, prone positioning has quickly become an important treatment strategy, however, prone positioning is labor intensive and comes with potential adverse effects. Therefore, identifying which critically ill intubated COVID-19 patients will benefit may help allocate labor resources. Methods From the multi-center Dutch Data Warehouse of COVID-19 ICU patients from 25 hospitals, we selected all 3619 episodes of prone positioning in 1142 invasively mechanically ventilated patients. We excluded...

Additional file 5. Supplementary Figure 4. Ratios of IL6+ B-effs to IL10+ B-regs in peripheral blood from healthy controls, smokers, and COPD patients determined by flow cytometry. Ratios of IL6+ B-effs to IL10+ B-regs within total B lymphocytes (A) and B cell subsets (B-E) are shown.

Additional file 9. Supplementary methods.

Additional file 1: Table S1. Overview of eligible patients from the SICS cohorts and RV function measurements obtained. Table S2. Overview of missing strain segments. Table S3. Baseline characteristics based on conventional RV function measurements preserved and strain reduced (CPSR). Table S4. RV echocardiography variables categorized by receiving mechanical ventilation (with peep > 8). Table S5. Baseline characteristics sensitivity analysis: RV fractional area change (FAC) preserved versus RV strain reduced (FPSR). Figure S1. Sensitivity analysis;...

Additional file 3: Figure S3. Effect of concentration and duration collagenase IV digestion of human brain tissue on the expression of T-cell differentiation markers. Normal-appearing white matter obtained from 3 deceased brain donors was digested with collagenase IV at different tissue weight to digestion medium volume ratios (i.e., 1:2.5; 1:5 or 1:10 w:v) and different digestion incubation times (i.e., 30, 60 or 120 min). Frequency of CD4+, CD8+, CD69+, CD103+, CD45RA+, CD27+, CCR7+, CD28+, KLRG1+,...

Additional file 2.

Additional file 2.

Additional file 1. Additional file containing supplemental figures and tables. Fig. S1. Flowchart of participants included in the study. Fig. S2. Manhattan plot of associations between a mixture of phthalates and bisphenols during first, second and third trimester with DNA methylation at birth. Table S1. Urine concentrations of phthalates and bisphenols during pregnancy in non-participants. Table S2. CpGs with p-values <1.0 * 10–5 from epigenome-wide association study of a mixture of phthalates and bisphenols in...

Additional file 1. Additional file containing supplemental figures and tables. Fig. S1. Flowchart of participants included in the study. Fig. S2. Manhattan plot of associations between a mixture of phthalates and bisphenols during first, second and third trimester with DNA methylation at birth. Table S1. Urine concentrations of phthalates and bisphenols during pregnancy in non-participants. Table S2. CpGs with p-values <1.0 * 10–5 from epigenome-wide association study of a mixture of phthalates and bisphenols in...

Additional file 1: Table S3. Summary of the updated 2022 guidelines for AMI: statements and recommendations.

Additional file 1. Appendix 1.

Additional file 2. Supplementary computational method. Equations, mathematical framework and justification of deviation from the general rule in the equations.

Additional file 2. Supplementary computational method. Equations, mathematical framework and justification of deviation from the general rule in the equations.

Additional file 3: Table S2. List of variables and mouse genes correspondence. All mathematical variables and the corresponding node in the network have names written in upper cases and do not reflect the official human or mouse nomenclatures. To relate those variables to actual genes more easily, we provide this table of correspondence. A related mouse gene name and NCBI ID is indicated for each variable. Nevertheless, this is not exhaustive since some variables represent...

Additional file 9: Data S4. In silico screening predictions of combinatorial treatments. (Microsoft Excel Worksheet). This file reports the results of a screening of combinatorial perturbations. More particularly, it reports all conditions that lead to a transition towards a healthy chondrocyte (SOX9+) when starting from a hypertrophic-like chondrocyte (Runx2+). The first sheet reports conditions leading to such transition 100% of the time, between 99 and 90% of the time for the next sheet and so...

Abstract Background Older patients using antihypertensive medication may experience Adverse Drug Events (ADEs), and thus benefit from deprescribing. The lack of a practical protocol may hamper deprescribing. Therefore, we aimed to develop a deprescribing protocol, based on a review of literature, combined with a feasibility test in a small number of patients. Methods A deprescribing protocol for general practitioners was drafted and tested in older patients using multiple antihypertensive medication in a single arm intervention....

Abstract Background Older patients using antihypertensive medication may experience Adverse Drug Events (ADEs), and thus benefit from deprescribing. The lack of a practical protocol may hamper deprescribing. Therefore, we aimed to develop a deprescribing protocol, based on a review of literature, combined with a feasibility test in a small number of patients. Methods A deprescribing protocol for general practitioners was drafted and tested in older patients using multiple antihypertensive medication in a single arm intervention....

Supplementary Material 1: Correlations between components of needs per health behavior

Additional file 10. Table that describes sample quality control statistics.

Additional file 11. Table that describes general information on DNAm predictors implemented.

Additional file 12. Peer review history.

Additional file 1: Supplementary figures 1-6.

Additional file 2. Table that describes full ICC statistics.

Additional file 4. Overview of all pipelines implemented with short descriptions.

Additional file 1. Final version of the Dutch Spine Oncology Study Group Outcomes Questionnaire 2.0 (SOSGOQ2.0).