Additional file 1. Detection results of clinical samples using LAMP-Microfluidic Chip.

Additional file 1. Detection results of clinical samples using LAMP-Microfluidic Chip.

Additional file 2. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for C. albicans.

Additional file 4. PCR primers of target genes.

Abstract Background Female reproductive tract infection (RTI) is the common source of varied diseases, especially as an important risk factor for pregnancy outcomes, therefore the rapid, accurate and simultaneous detection of multiple pathogens is in urgent need for assisting the diagnosis and treatment of RTI in pregnant women. Streptococcus agalactiae (S. agalactiae), Enterococcus faecalis (E. faecalis), Gardnerella vaginalis (G. vaginalis), Candida albicans (C. albicans) and Chlamydia trachomatis (C. trachomatis) are five main pathogens in lower...

Additional file 1: Table S1. Name, accession number, sequences, amplicon length of primer pairs used in the present experiment, efficiency of amplification of PCR, and references. Fig. S1. Relative mRNA expression level in the goat mammary epithelial cells incubated with adenovirus expression liver X receptor beta (LXRB). Fig. S2. Relative mRNA expression level in the goat mammary epithelial cells incubated siRNA targeted liver X receptor beta (LXRB).

Additional file 2. PRECIS-2 scores of chaiqin chengqi decoction for predicted moderately severe and severe acute pancreatitis (CAP trial) domains.

Abstract Background Mountains of the world host a significant portion of all terrestrial biodiversity, and the region of the Qinghai-Tibet Plateau (QTP) stands as one of the most remarkable mountain regions on Earth. Because many explosive radiations occurred there, the QTP is a natural laboratory which is ideal to investigate patterns and processes linked to speciation and diversification. Indeed, understanding how closely related and sympatric species diverged is vital to explore drivers fostering speciation, a...

Abstract Background In rodents, research has revealed a role of liver X receptors (LXR) in controlling lipid homeostasis and regulating the synthesis of polyunsaturated fatty acids (PUFA). Recent data suggest that LXRB is the predominant LXR subtype in ruminant mammary cells, but its role in lipid metabolism is unknown. It was hypothesized that LXRB plays a role in lipid homeostasis via altering the synthesis of PUFA in the ruminant mammary gland. We used overexpression and...

Supplementary Material 1. Changes in the DWRT and IWRT scores and memory cognitive impairment during an average follow-up of 4.1 years by baseline alcohol use and age group

Supplementary Material 1. Changes in the DWRT and IWRT scores and memory cognitive impairment during an average follow-up of 4.1 years by baseline alcohol use and age group

Additional file 1. Additional file showed the performance with 95% CI of all 84 combinations of joint modeling strategy.

Abstract Background Outliers and class imbalance in medical data could affect the accuracy of machine learning models. For physicians who want to apply predictive models, how to use the data at hand to build a model and what model to choose are very thorny problems. Therefore, it is necessary to consider outliers, imbalanced data, model selection, and parameter tuning when modeling. Methods This study used a joint modeling strategy consisting of: outlier detection and removal,...

Additional file 4: Fig. S1. Expressed lncRNAs in two crosses.

Additional file 6: Fig. S3. DNA methylation profiles of long non-coding RNAs (lncRNAs) and protein-coding genes (PCgenes) in sunflower endosperm from SY1.

Additional file 9: Fig. S5. Identification of imprinted long non-coding RNAs (lncRNAs) in sunflower endosperm at 12 DAP.

Additional file 9: Fig. S5. Identification of imprinted long non-coding RNAs (lncRNAs) in sunflower endosperm at 12 DAP.

Abstract Background Long non-coding RNAs (lncRNAs), as important regulators, play important roles in plant growth and development. The expression and epigenetic regulation of lncRNAs remain uncharacterized generally in plant seeds, especially in the transient endosperm of the dicotyledons. Results In this study, we identified 11,840 candidate lncRNAs in 12 day-after-pollination sunflower endosperm by analyzing RNA-seq data. These lncRNAs were evenly distributed in all chromosomes and had specific features that were distinct from mRNAs including tissue-specificity...

Abstract Background Infection is one of the most common causes of death in patients with hematological malignancies during chemotherapy. Due to its special location, local warmth and humidity, repeated pollution with stool and urine, and characteristically wrinkled anatomical structure within which bacteria can hide, the perianal becomes a site with a high incidence of infection. Such infection also has a high recurrence rate and high mortality, increasing the economic burden of patients, delaying the time...

Additional file 7: Table S6. RNA-seq data of the 14 differential expressed genes associated with nitrogen utilization.

Additional file 1: Text S1. Members of the China Kadoorie Biobank Collaborative Group. Text S2. Questionnaire for the sleep patterns in the survey of China Kadoorie Biobank. Table S1. Associations between Baseline Sleep Patterns and Frailty Transitions among Participants Aged < 60. Table S2. Associations between Baseline Sleep Patterns and Frailty Transitions Adjusted for Major Diseases and Medication. Table S3. Associations between Constantly Healthy Sleep Patterns and Frailty Transitions Adjusted for Major Diseases and Medication....

Additional file 1: Text S1. Members of the China Kadoorie Biobank Collaborative Group. Text S2. Questionnaire for the sleep patterns in the survey of China Kadoorie Biobank. Table S1. Associations between Baseline Sleep Patterns and Frailty Transitions among Participants Aged < 60. Table S2. Associations between Baseline Sleep Patterns and Frailty Transitions Adjusted for Major Diseases and Medication. Table S3. Associations between Constantly Healthy Sleep Patterns and Frailty Transitions Adjusted for Major Diseases and Medication....

Pre-eclampsia is a serious pregnancy complication and a major global concern for the mortality of both mother and fetus. Existing symptomatic treatments do not delay disease progression; thus, timely delivery of the baby is the most effective measure. However, the risk of various maternal and fetal injuries remains. In this review, we summarize potential strategies for pharmacologic interventions in pre-eclamptic therapy. Specifically, we discuss the pathophysiological process of various effective candidate therapeutics that act on...

Abstract Resolvin D1 (RvD1) has been shown to provide effective protection against ischemia–reperfusion injury in multiple vital organs such as the heart, brain, kidney. However, the clinical translational potential of systemic administration of RvD1 in the treatment of ischemia–reperfusion injury is greatly limited due to biological instability and lack of targeting ability. Combining the natural inflammatory response and reactive oxygen species (ROS) overproduction after reperfusion injury, we developed a platelet-bionic, ROS-responsive RvD1 delivery platform. The...

Additional file 1: Figure S1. Stability of nanovesicles in PBS and PBS with 20% of fetal bovine serum (FBS). Nanovesicle sizes were measured using dynamic light scattering (n=3 per group). Results are presented as mean ± SD. Figure S2. The promotion of PLP-RvD1 treated macrophages to angiogenesis. (A) Capillary tube formation and (B) cell migration of HUVECs after cocultured with PBS, LP-RvD1 or PLP-RvD1 treated BMDMs. HUVECs cultured under normoxia were set as controls. Scalar...