Pieces in a global puzzle: Population genetics at two whale shark aggregations in the western Indian OceanRoyale Hardenstine, Song He, Jesse Cochran, Camrin Braun, E. Fernando Cagua, Simon Pierce, Clare Prebble, Christoph Rohner, Pablo Saenz-Agudelo, Tane Sinclair-Taylor, Gregory Skomal, Simon Thorrold, Alexandra Watts, Casey Zakroff & Michael Berumen
The whale shark Rhincodon typus is found throughout the world’s tropical and warm-temperate ocean basins. Despite their broad physical distribution, research on the species has been concentrated at a few aggregation sites. Comparing DNA sequences from sharks at different sites can provide a demographically neutral understanding of the whale shark’s global ecology. Here, we created genetic profiles for 84 whale sharks from the Saudi Arabian Red Sea and 72 individuals from the coast of Tanzania...
Moderate pH deviations trigger transcriptome-wide responses in human cells cultured in physioxia and physiological buffersSilvia Arossa, Samhan M Alsolami, Shannon Grace Klein, Alexandra Steckbauer, Anieka Janice Parry, Yingzi Zhang, Gerardo Ramos Mandujano, Juan Carlos Izpisua-Belmonte, Carlos Manauel Duarte & Mo Li
Although pH instability is emerging as a potential driver of changes in cell physiology, pH is still poorly controlled during cell culture and in vitro experiments. Standard procedures include the use of chemicals usually not present in the primary physiological buffering system (CO2/HCO3-), such as acids and bases, to manipulate pH levels. This, however, leads to artifacts that potentially affect scientists’ findings. Here, we propose a novel method for controlling pH levels by relying only...
Data from: Applied phenomics and genomics for improving barley yellow dwarf resistance in winter wheatPaula Silva, Byron Evers, Alexandria Kieffaber, Xu Wang, Allan Fritz, Jared Crain, Jesse Poland & Liangliang Gao
Barley yellow dwarf is one of the major viral diseases of cereals. Phenotyping barley yellow dwarf in wheat is extremely challenging due to similarities to other biotic and abiotic stresses. Breeding for resistance is additionally challenging as the wheat primary germplasm pool lacks genetic resistance, with most of the few resistance genes named to date originating from a wild relative species. The objectives of this study were to (1) evaluate the use of high-throughput phenotyping...
Carbon isotope fingerprinting, or multivariate analysis using δ13C values of individual compounds, is a powerful tool in ecological studies, particularly measurements of essential amino acids (EAA δ13C). Despite the widespread application of this technique, there has been little methodological validation to determine (1) whether multivariate EAA δ13C signatures (fingerprints) of primary producer groups vary across space and time, and (2) what biochemical mechanisms drive these patterns. Here, we evaluate the spatiotemporal consistency in EAA δ13C...
Understanding how animals move in dense environments where vision is compromised is a major challenge. We used GPS and dead-reckoning to examine the movement of Magellanic penguins commuting through vegetation that precluded long-distance vision. Birds leaving the nest followed the shortest, quickest route to the sea (the ‘ideal path’ [I-path]) but return tracks depended where the birds left the water. Penguins arriving at the beach departure spot mirrored the departure. Most of those landing at...
King Abdullah University of Science and Technology5
University of Buenos Aires1
Marine Megafauna Foundation1
Austral University of Chile1
Instituto Nacional de Investigación Agropecuaria1
University of Florida1
National Marine Fisheries Service1
University of California, Riverside1
Australian Institute of Marine Science1
Salk Institute for Biological Studies1