Cellular plasticity in response to suppression of storage proteins in the Brassica napus embryo

Hardy Rolletschek, Jörg Schwender, Christina König, Kent D. Chapman, Trevor Romsdahl, Christin Lorenz, Hans-Peter Braun, Peter Denolf, Katrien Van Audenhove, Eberhard Munz, Nicolas Heinzel, Stefan Ortleb, Twan Rutten, Sean McCorkle, Taras Borysyuk, André Guendel, Hai Shi, Michiel Vander Auwermeulen, Stephane Bourot & Ljudmilla Borisjuk
The trade-off between protein and oil storage in oilseed crops has been tested here in oilseed rape (Brassica napus) by analyzing the effect of suppressing key genes encoding protein storage products (napin, cruciferin). The phenotypic outcomes were assessed using nuclear magnetic resonance and mass spectrometry imaging, microscopy, transcriptomics, proteomics, metabolomics, lipidomics, immunological assays as well as by flux balance analysis. Surprisingly, the profile of storage products was only moderately changed in RNAi transgenics. However, embryonic...

Registration Year

  • 2020
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Resource Types

  • Dataset
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Affiliations

  • University of North Texas
    1
  • BASF (Germany)
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  • Brookhaven National Laboratory
    1
  • Institute of Plant Genetics and Crop Plant Research
    1
  • University of Hannover
    1