453 Works

Large-scale quantitative genomics analyzes the circRNA expression profile and identifies the key circRNA in regulating cell proliferation during the proliferation phase of rat LR

Xueqiang Guo, Wei Jin, Cuifang Chang, Yi Ding, Yahao Wang, Lifei Li, Yanhui Chen, Jingbo Zhang, Cunshuan Xu, Guangwen Chen & Jianlin Guo
Researchers have been exploring the genetic mechanisms underlying the control of liver regeneration (LR). However, an integrated analysis of circRNAs expression of rat regenerating livers during the proliferation phase has not been performed yet. For this purpose, circRNAs expression profile was globally analyzed by high-throughput sequencing. It showed that 10,003 circRNAs were detected, and 164 circRNAs were differentially expressed. Subsequently, 27 circRNAs were predicted to bind to 58 candidate miRNAs and compete for miRNA-binding sites...

Large-scale quantitative genomics analyzes the circRNA expression profile and identifies the key circRNA in regulating cell proliferation during the proliferation phase of rat LR

Xueqiang Guo, Wei Jin, Cuifang Chang, Yi Ding, Yahao Wang, Lifei Li, Yanhui Chen, Jingbo Zhang, Cunshuan Xu, Guangwen Chen & Jianlin Guo
Researchers have been exploring the genetic mechanisms underlying the control of liver regeneration (LR). However, an integrated analysis of circRNAs expression of rat regenerating livers during the proliferation phase has not been performed yet. For this purpose, circRNAs expression profile was globally analyzed by high-throughput sequencing. It showed that 10,003 circRNAs were detected, and 164 circRNAs were differentially expressed. Subsequently, 27 circRNAs were predicted to bind to 58 candidate miRNAs and compete for miRNA-binding sites...

MiR-199a-5p regulates rat liver regeneration and hepatocyte proliferation by targeting TNF-α TNFR1/TRADD/CASPASE8/CASPASE3 signalling pathway

Chunyan Zhang, Bingyu Ye, Jiaojiao Wei, Qiwen Wang, Cunshuan Xu & Guoying Yu
Abnormally expressed miR-199a-5p (miR-199a) has been frequently reported in multiple types of malignancies. Nevertheless, its effect in liver regeneration (LR) is largely still unclear. Herein, we investigated the function of miR-199a in hepatocyte proliferation during LR. As a result, miR-199a expression was significantly increased 12–30 h, in rat hepatic tissue, after partial hepatectomy (PH). The down-regulated expression of miR-199a inhibited proliferation as well as promoted cell apoptosis of BRL-3A. Additionally, TNF-α was found as a...

Data from: Optimization of nitrogen and carbon removal with simultaneous partial nitrification, anammox, and denitrification (SNAD) in membrane bioreactor (MBR)

Zhang Kai, Wang Zhaozhao, Sun Mengxia, Liang Dongbo, Hou Liangang, Zhang Jing, Wang Xiujie & Li Jun
In this study, a membrane bioreactor (MBR) was used to achieve both nitrogen and carbon removal by a simultaneous partial nitrification, anammox, and denitrification (SNAD) process. During the entire experiment, the optimal operating parameters as determined by response surface methodology (RSM) were a C/N value of 1.16, a DO value of 0.84 mg·L-1, and an aerobic time (Tae) of 15.75 min. Under these conditions, the SNAD process achieved efficient and stable nitrogen and carbon removal;...

Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy

Mingming Song, Wentao Xia, Zixuan Tao, Bin Zhu, Wenxiang Zhang, Chang Liu & Siyu Chen
Malignant melanoma is a life-threatening form of skin cancer with a low response rate to single-agent chemotherapy. Although combined therapies of metformin (MET) and doxorubicin (DOX) are effective in treating a variety of cancers, including breast cancer, their different physicochemical properties and administration routines reduce the effective co-accumulation of both drugs in tumors. Nanoparticles (NPs) have been demonstrated to potentially improve drug delivery efficiency in cancer therapy of, for example, liver and lung cancers. Hence,...

Comparison of different pretreatment methods on phosphorus release and recovery as struvite from excess sludge

Dexiu Hu, Cong Zhang & Yan Zhang
Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

Comparison of different pretreatment methods on phosphorus release and recovery as struvite from excess sludge

Dexiu Hu, Cong Zhang & Yan Zhang
Recovering phosphorus (P) from excess sludge of wastewater treatment plants (WWTPs) has attracted considerable attention. An efficient P release method is undoubtedly critical for a satisfactory recovery performance. In this study, the effectiveness of three sludge pretreatment methods, i.e. anaerobic digestion (AD), EDTA-anaerobic digestion (EA) and ultrasound combined with EA (U-EA), on P release and struvite recovery from excess sludge was investigated. The results showed that different pretreatment methods resulted in the different characteristics of...

Comprehensive analysis of pivotal biomarkers, immune cell infiltration and therapeutic drugs for steroid-induced osteonecrosis of the femoral head

Bo Wang, Song Gong, Wenkai Shao, Lizhi Han, Zilin Li, Zhichao Zhang, Yang Zheng, Fang Ouyang, Yan Ma, Weihua Xu & Yong Feng
Steroid-induced osteonecrosis of the femoral head (SONFH) is a progressive disease that leads to an increased disability rate. This study aimed to ascertain biomarkers, infiltrating immune cells, and therapeutic drugs for SONFH. The gene expression profile of the GSE123568 dataset was downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified using the NetworkAnalyst platform. Functional enrichment, protein-protein interaction network (PPI), and module analyses were performed using Metascape tools. An...

Eukaryotic translation initiation factor 3 subunit B could serve as a potential prognostic predictor for breast cancer

Shaoran Song, Jie Liu, Miao Zhang, Xiaoqian Gao, Wei Sun, Peijun Liu, Yaochun Wang & Juan Li
The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

Eukaryotic translation initiation factor 3 subunit B could serve as a potential prognostic predictor for breast cancer

Shaoran Song, Jie Liu, Miao Zhang, Xiaoqian Gao, Wei Sun, Peijun Liu, Yaochun Wang & Juan Li
The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

Sevoflurane reduces lipopolysaccharide-induced apoptosis and pulmonary fibrosis in the RAW264.7 cells and mice models to ameliorate acute lung injury by eliminating oxidative damages

Fushuang Zheng, Xiuying Wu, Jin Zhang, Zhiling Fu & Yan Zhang
Sevoflurane is identified as an effective candidate drug for acute lung injury (ALI) treatment, but its curing effects and detailed mechanisms have not been fully disclosed. The present study was designed to resolve this academic issue. The ALI mice models were established, and Hematoxylin-eosin staining assay was performed to examine tissue morphologies. Cell viability was determined by CCK-8 assay, and Annexin V-FITC/PI double staining assay was used to examine cell apoptosis. The expression levels of...

Design, synthesis, biological evaluation and molecular docking studies of novel pleuromutilin derivatives containing nitrogen heterocycle and alkylamine groups

Qi Wang, Jie Liu, Zi-Dan Zhou, Ke-Xin Zhou, Fei Li, Qi-Wen Zhang, Shou-Kai Wang, Wei Wang, Zhen Jin & You-Zhi Tang
A series of pleuromutilin derivatives containing alkylamine and nitrogen heterocycle groups were designed and synthesised under mild conditions. The in vitro antibacterial activity of these semisynthetic derivatives against four strains of Staphylococcus aureus (MRSA ATCC 43300, S.aureus ATCC 29213, S.aureus AD3, and S.aureus 144) were evaluated by the broth dilution method. Compound 13 was found to have excellent antibacterial activity against MRSA (MIC = 0.0625 μg/mL). Furthermore, compound 13 was further studied by the time-killing...

Additional file 4 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes

Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 4. miRNA sequences.

Additional file 5 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes

Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 5. Coding Sequence of Irak1 and Traf6 Genes.

Additional file 8 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes

Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 8. The other 4 abundant miRNAs mimics’ effects on mRNA expression of neurotoxic astrocyte markers. Quantitative analysis of mRNA expression of C3 and Lcn2 in astrocytes, neurotoxic astrocytes and neurotoxic astrocytes treated with 50 nM mimics of miR-21-5p(a), miR-22-3p(b), miR-24-3p(c), miR-26a-5p(d) by RT-QPCR. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01; #, p>0.05. C3 complement c3, Lcn2 lipocalin-2.

Additional file 1 of A genetic map of the chromatin regulators to drug response in cancer cells

Bo Chen, Pengfei Li, Mingyue Liu, Kaidong Liu, Min Zou, Yiding Geng, Shuping Zhuang, Huanhuan Xu, Linzhu Wang, Tingting Chen, Yawei Li, Zhangxiang Zhao, Lishuang Qi & Yunyan Gu
Additional file 1: Fig. S1. Lung cancer cell lines with RB1 mutations are resistant to CDK4/6 inhibitor, Fig. S2. Sensitive biomarkers interacted with CHEK1 in large intestine cancer cell lines, Fig. S3. Resistant biomarkers interacted with CHEK1 in lung cancer cell lines, Fig. S4. Functional analysis of the CRs genetic interaction, Fig. S5. Differential chromatin accessibility for CSL interactions, Fig. S6. Differential chromatin accessibility for CSV interactions, Fig. S7. TUBA1C subnetwork in drug related CSL...

Additional file 2 of A genetic map of the chromatin regulators to drug response in cancer cells

Bo Chen, Pengfei Li, Mingyue Liu, Kaidong Liu, Min Zou, Yiding Geng, Shuping Zhuang, Huanhuan Xu, Linzhu Wang, Tingting Chen, Yawei Li, Zhangxiang Zhao, Lishuang Qi & Yunyan Gu
Additional file 2: Table S3. CRs genetic interactions related to drug response in cancers.

Maintenance mechanisms of child restraint system use based on the health action process approach theory: Evidence from China

Kangkang Zhang, Li Liu, Ting Zhang, Chi Zhang, Xinpeng Xu, Qifeng Wu & Hua You
Adherence to the use of child restraint system (CRS) can greatly reduce child casualties in road traffic accidents. This study aims to develop the behavior maintenance model of CRS use based on the health action process approach (HAPA) theory to explore the maintenance mechanisms, suggesting appropriate intervention strategies. Adopting the multi-stage stratified cluster random sampling method, a cross-sectional survey for guardians recruited from kindergarten and primary school was conducted using the questionnaire developed based on...

Additional file 2 of The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Zili Li, Fang Fang, Yuting Long, Qian Zhao, Xiaotong Wang, Zhen Ye, Tianqing Meng, Xiuli Gu, Wenpei Xiang, Chengliang Xiong & Honggang Li
Additional file 2: Fig. S2. The 5hmC/5mC Levels and Growth Curves in TET Gene Knockout hESCs, Related to Fig. 1. (A) Analysis of 5hmC and 5mC levels in each cell line by dot blot; (B) Analysis of 5hmC and 5mC levels in WT, TKO and TET2/3 DKO TET1 heterozygote cell lines by dot blot; (C) Growth curves for WT and TKO hESCs. Error bars indicate mean ± s.d. from three independent biological replicates.

Additional file 2 of The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Zili Li, Fang Fang, Yuting Long, Qian Zhao, Xiaotong Wang, Zhen Ye, Tianqing Meng, Xiuli Gu, Wenpei Xiang, Chengliang Xiong & Honggang Li
Additional file 2: Fig. S2. The 5hmC/5mC Levels and Growth Curves in TET Gene Knockout hESCs, Related to Fig. 1. (A) Analysis of 5hmC and 5mC levels in each cell line by dot blot; (B) Analysis of 5hmC and 5mC levels in WT, TKO and TET2/3 DKO TET1 heterozygote cell lines by dot blot; (C) Growth curves for WT and TKO hESCs. Error bars indicate mean ± s.d. from three independent biological replicates.

Additional file 5 of The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Zili Li, Fang Fang, Yuting Long, Qian Zhao, Xiaotong Wang, Zhen Ye, Tianqing Meng, Xiuli Gu, Wenpei Xiang, Chengliang Xiong & Honggang Li
Additional file 5: Fig. S5. TET Gene Knockout Shows No Effect on iMeLCs Induction, Related to Fig. 1. (A) Bright filed of iMeLCs induction from WT and TKO hESCs (42 h); (B) RT-qPCR analysis for each gene during iMeLCs differentiation in 42 h; n = 3 independent experiments. Data are presented as means ± s.d; (C) Vectors for overexpression of DOX-inducible SOX17, and TMP-inducible NANOG transgenes in BLIMP1–mKate2 reporter hESCs.

Additional file 11 of Lipidomics combined with transcriptomic and mass spectrometry imaging analysis of the Asiatic toad (Bufo gargarizans) during metamorphosis and bufadienolide accumulation

Bo Sun, Shan Jiang, Mingli Li, Yan Zhang, Yanyan Zhou, Xiaolu Wei, Hongjie Wang, Nan Si, Baolin Bian & Haiyu Zhao
Additional file 11: Fig. S4. KEGG enrichment bubble diagram (G31 vs. G38, G38 vs. G32, G42 vs. G46). (A) and (B) showed the top 30 pathways of significant enrichment of the up-and down- regulated DEGs on KEGG for G31 vs. G38, (C) and (D) showed for G38 vs. G42, and (E) and (F) showed for G42 vs. G46.

Additional file 15 of Lipidomics combined with transcriptomic and mass spectrometry imaging analysis of the Asiatic toad (Bufo gargarizans) during metamorphosis and bufadienolide accumulation

Bo Sun, Shan Jiang, Mingli Li, Yan Zhang, Yanyan Zhou, Xiaolu Wei, Hongjie Wang, Nan Si, Baolin Bian & Haiyu Zhao
Additional file 15: Fig. S8. Enrichment analysis of DEGs for BD accumulation. (A) By comparing G46 with G31, G38 and G42 respectively, 3260 common DEGs were identified. (B) and (C) showed the top 30 GO terms and pathways of significant enrichment of the BDs-related DEGs.

Additional file 4 of The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Zili Li, Fang Fang, Yuting Long, Qian Zhao, Xiaotong Wang, Zhen Ye, Tianqing Meng, Xiuli Gu, Wenpei Xiang, Chengliang Xiong & Honggang Li
Additional file 4: Fig. S4. RT-qPCR analysis for SOX17, BLIMP1, TFAP2C, NANOS3, POU5F1, SOX2 during hPGC differentiation in day 4 embryoids, Related to Fig. 1; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by Student’s t-test (two-sided), compared to WT group, *p < 0.05, **p < 0.01, ***p < 0.001, Related to Fig. 1.

Additional file 10 of Lipidomics combined with transcriptomic and mass spectrometry imaging analysis of the Asiatic toad (Bufo gargarizans) during metamorphosis and bufadienolide accumulation

Bo Sun, Shan Jiang, Mingli Li, Yan Zhang, Yanyan Zhou, Xiaolu Wei, Hongjie Wang, Nan Si, Baolin Bian & Haiyu Zhao
Additional file 10: Fig. S3. GO enrichment bubble diagram (G31 vs. G38, G38 vs. G42, G42 vs. G46). (A) and (B) showed the top 30 pathways of significant enrichment of the upregulated and downregulated DEGs on GO for G31 vs. G38, (C) and (D) showed for G38 vs. G42, and (E) and (F) showed for G42 vs. G46.

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