The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

Sevoflurane is identified as an effective candidate drug for acute lung injury (ALI) treatment, but its curing effects and detailed mechanisms have not been fully disclosed. The present study was designed to resolve this academic issue. The ALI mice models were established, and Hematoxylin-eosin staining assay was performed to examine tissue morphologies. Cell viability was determined by CCK-8 assay, and Annexin V-FITC/PI double staining assay was used to examine cell apoptosis. The expression levels of...

Additional file 4. miRNA sequences.

Additional file 5. Coding Sequence of Irak1 and Traf6 Genes.

Additional file 8. The other 4 abundant miRNAs mimics’ effects on mRNA expression of neurotoxic astrocyte markers. Quantitative analysis of mRNA expression of C3 and Lcn2 in astrocytes, neurotoxic astrocytes and neurotoxic astrocytes treated with 50 nM mimics of miR-21-5p(a), miR-22-3p(b), miR-24-3p(c), miR-26a-5p(d) by RT-QPCR. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01; #, p>0.05. C3 complement c3, Lcn2 lipocalin-2.

Additional file 1: Fig. S1. Lung cancer cell lines with RB1 mutations are resistant to CDK4/6 inhibitor, Fig. S2. Sensitive biomarkers interacted with CHEK1 in large intestine cancer cell lines, Fig. S3. Resistant biomarkers interacted with CHEK1 in lung cancer cell lines, Fig. S4. Functional analysis of the CRs genetic interaction, Fig. S5. Differential chromatin accessibility for CSL interactions, Fig. S6. Differential chromatin accessibility for CSV interactions, Fig. S7. TUBA1C subnetwork in drug related CSL...

Additional file 2: Table S3. CRs genetic interactions related to drug response in cancers.

Potentilla kleiniana Wight et Arn（PK, ‘Wu Pi Feng’ in Chinese） was recorded as Miao ethnic medicine for treatment of fever, cough, ulcer, and erysipelas for thousands years. This study aimed to evaluate the antiviral activity of four PK extracts and seven compounds by using HIV-1 protease (HIV-1 PR). In addition, Ultra-High Performance Liquid Chromatography and High Resolution Mass Spectrometry (UPLC-HRMS) was employed to identify the bioactive components. The toxicity assessment of the extracts was done...

Additional file 10. Table S7. Recurrent CNVs in previously published syndromic CHD cohorts and our study.

Additional file 2. Table S2. Training gene set generated from RDDC, Phenopedia, and DisGeNET.

Abstract Background Brain functional abnormalities at rest have been observed in obsessive–compulsive disorder (OCD). However, whether and how anatomical distance influences functional connectivity (FC) at rest is ambiguous in OCD. Methods Using resting-state functional magnetic resonance imaging data, we calculated the FC of each voxel in the whole-brain and divided FC into short- and long-range FCs in 40 medicine-free patients with OCD and 40 healthy controls (HCs). A support vector machine (SVM) was used to...

supplementary materials

Additional file 7: Table S7. Differential lipids related DEGs (TOP 500).

Abstract Background As health care cost is taking an increasingly substantial proportion of national wealth, health shocks and the subsequent medical expenditures have become increasingly vital contributions to financial risks. However, the individual or combined effects of social and financial medical insurance on household financial behaviors are poorly understood. This research aims to examine the effect of health shocks on financial asset mobility and portfolio allocation of the household. Also, whether medical insurance positively affects...

Supplementary Material 1

Additional file 1. Table S1. Rice importins retrieved from Rice Genome Annotation Project databases. Table S2. Early characterizations of OsIMPα1a-edited (OsIMPα1a-ED) and OsIMPα1b-edited (OsIMPα1b-ED) rice lines. Table S3. Information on genes tested and primers used in this study.

A phytochemical investigation of Menispermum dauricum led to the isolation of five oxoisoaporphine-type alkaloids (1–5) and five aporphine-type alkaloids (6–10), including a novel oxoisoaporphine-type alkaloid: menispeimin A (1). Their structures were elucidated by spectroscopic studies including MS, 1 D and 2 D NMR, and confirmed by comparing with literature data. Among them, alkaloids 4–10 were obtained for the first time from Menispermum genus. Natural products 2, 4 and 6 exhibited significant cytotoxic activity against A549,...

Two new sesquiterpenoids, flammupin A (1) and flammupin B (2), along with two known compounds, enokipodin C (3) and 5,5'-dibuthoxy-2,2'-bifuran (4) were obtained from Flammulina velutipes, an endophytic fungus isolated from the roots of Caulophyllum robustum Maxim. The structures were elucidated by the combination of HR-ESI-MS, NMR, and ECD analyses. Compound 3 exhibited moderate to potent cytotoxicity against A549, HeLa, and SMMC-7721 cells with IC50 values ranged from 3.69 to 11.84 μM.

Adherence to the use of child restraint system (CRS) can greatly reduce child casualties in road traffic accidents. This study aims to develop the behavior maintenance model of CRS use based on the health action process approach (HAPA) theory to explore the maintenance mechanisms, suggesting appropriate intervention strategies. Adopting the multi-stage stratified cluster random sampling method, a cross-sectional survey for guardians recruited from kindergarten and primary school was conducted using the questionnaire developed based on...

Additional file 2: Fig. S2. The 5hmC/5mC Levels and Growth Curves in TET Gene Knockout hESCs, Related to Fig. 1. (A) Analysis of 5hmC and 5mC levels in each cell line by dot blot; (B) Analysis of 5hmC and 5mC levels in WT, TKO and TET2/3 DKO TET1 heterozygote cell lines by dot blot; (C) Growth curves for WT and TKO hESCs. Error bars indicate mean ± s.d. from three independent biological replicates.

Additional file 2: Fig. S2. The 5hmC/5mC Levels and Growth Curves in TET Gene Knockout hESCs, Related to Fig. 1. (A) Analysis of 5hmC and 5mC levels in each cell line by dot blot; (B) Analysis of 5hmC and 5mC levels in WT, TKO and TET2/3 DKO TET1 heterozygote cell lines by dot blot; (C) Growth curves for WT and TKO hESCs. Error bars indicate mean ± s.d. from three independent biological replicates.

Additional file 5: Fig. S5. TET Gene Knockout Shows No Effect on iMeLCs Induction, Related to Fig. 1. (A) Bright filed of iMeLCs induction from WT and TKO hESCs (42 h); (B) RT-qPCR analysis for each gene during iMeLCs differentiation in 42 h; n = 3 independent experiments. Data are presented as means ± s.d; (C) Vectors for overexpression of DOX-inducible SOX17, and TMP-inducible NANOG transgenes in BLIMP1–mKate2 reporter hESCs.

Additional file 11: Fig. S4. KEGG enrichment bubble diagram (G31 vs. G38, G38 vs. G32, G42 vs. G46). (A) and (B) showed the top 30 pathways of significant enrichment of the up-and down- regulated DEGs on KEGG for G31 vs. G38, (C) and (D) showed for G38 vs. G42, and (E) and (F) showed for G42 vs. G46.

Additional file 15: Fig. S8. Enrichment analysis of DEGs for BD accumulation. (A) By comparing G46 with G31, G38 and G42 respectively, 3260 common DEGs were identified. (B) and (C) showed the top 30 GO terms and pathways of significant enrichment of the BDs-related DEGs.