6,181 Works

Additional file 1 of Comparative study of excretory–secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata

Conor E. Fogarty, Min Zhao, Donald P. McManus, Mary G. Duke, Scott F. Cummins & Tianfang Wang
Additional file 1: Database S1. The B. glabrata protein database used in the proteomic data analysis.

SPA: a peptide antagonist that acts as a cell-penetrating peptide for drug delivery

Jingjing Song, Sujie Huang, Zhengzheng Zhang, Bo Jia, Huan Xie, Ming Kai & Wei Zhang
Although cell-penetrating peptides (CPPs) has been proven to be efficient transporter for drug delivery, ideal peptide vectors for tumor therapy are still being urgently sought. Peptide antagonists have attracted substantial attention as targeting molecules because of their high tumor accumulation and antitumor activity compared with agonists. SPA, a derivative of substance P, is a potent antagonist that exhibits antitumor activity. Based on the amino acid composition of SPA, we speculate that it can translocate across...

Myotubularin related protein 7 is essential for the spermatogonial stem cell homeostasis via PI3K/AKT signaling

Dan Zhao, Cong Shen, Tingting Gao, Hong Li, Yueshuai Guo, Feng Li, Chenchen Liu, Yuanyuan Liu, Xia Chen, Xi Zhang, Yangyang Wu, Yi Yu, Meng Lin, Yan Yuan, Xiaofang Chen, Xiaoyan Huang, Shenmin Yang, Jun Yu, Jun Zhang & Bo Zheng
Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis and tumor growth. We have previously identified MTMR7 in the proteomic profile of mouse spermatogonial stem cell (SSC) maturation and differentiation, implying that MTMR7 is associated with neonatal testicular development. In this study, to further explore the distribution and function of MTMR7 in mouse testis, we studied the effect of Mtmr7 knockdown on neonatal testicular...

Bicomponent polymeric micelles for pH-controlled delivery of doxorubicin

Chunyun Wang, Peilan Qi, Yan Lu, Lei Liu, Yanan Zhang, Qianli Sheng, Tianshun Wang, Mengying Zhang, Rui Wang & Shiyong Song
Stimuli-responsive drug delivery systems (DDSs) are expected to realize site-specific drug release and kill cancer cells selectively. In this study, a pH-responsive micelle was designed utilizing the pH-sensitivity of borate bonds formed between dopamine and boronic acid. First, methyl (polyethylene glycol)-block-polycaprolactone (mPEG-PCL) was conjugated with 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTP) to obtain a macroinitiator. Two different segments poly(dopamine methacrylamide) (PDMA) and poly(vinylphenylboronic acid) (PVBA) were then grafted to the end of mPEG-PCL. Two triblock copolymers, mPEG-PCL-PDMA and...

Cholesterol depletion sensitizes gallbladder cancer to cisplatin by impairing DNA damage response

Yonglong Zhang, Yanfeng Liu, Jinlin Duan, Hui Wang, Yuchen Zhang, Ke Qiao & Jian Wang
Gallbladder cancer (GBC) is the common malignancy of the bile tract system with extremely poor clinical outcomes, owing to its metastatic property and intrinsic resistance to the first-line drugs. Although it is well-established that cholesterol abnormity contributes to gallstone formation, a leading risk factor for GBC, the link of cholesterol homeostasis with GBC has not been investigated. The present study systematically examined the genes implicated in cholesterol homeostasis, and revealed altered gene expressions of de...

Improved solubility, dissolution rate, and oral bioavailability of main biflavonoids from Selaginella doederleinii extract by amorphous solid dispersion

Bing Chen, Xuewen Wang, Yanyan Zhang, Kangping Huang, Hao Liu, Dafen Xu, Shaoguang Li, Qicai Liu, Jianyong Huang, Hong Yao & Xinhua Lin
Amentoflavone, robustaflavone, 2″,3″-dihydro-3′,3‴-biapigenin, 3′,3‴-binaringenin, and delicaflavone are five major hydrophobic components in the total biflavonoids extract from Selaginella doederleinii (TBESD) that display favorable anticancer properties. The purpose of this study was to develop a new oral delivery formulation to improve the solubilities, dissolution rates, and oral bioavailabilities of the main ingredients in TBESD by the solid dispersion technique. Solid dispersions of TBESD with various hydrophilic polymers were prepared, and different technologies were applied to select...

Renin-angiotensin system inhibitors improve the clinical outcomes of COVID-19 patients with hypertension

Juan Meng, Guohui Xiao, Juanjuan Zhang, Xing He, Min Ou, Jing Bi, Rongqing Yang, Wencheng Di, Zhaoqin Wang, Zigang Li, Hong Gao, Lei Liu & Guoliang Zhang
The dysfunction of the renin-angiotensin system (RAS) has been observed in coronavirus infection disease (COVID-19) patients, but whether RAS inhibitors, such as angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin II type 1 receptor blockers (ARBs), are associated with clinical outcomes remains unknown. COVID-19 patients with hypertension were enrolled to evaluate the effect of RAS inhibitors. We observed that patients receiving ACEI or ARB therapy had a lower rate of severe diseases and a trend toward a...

The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals

Yuqiang Zhang, Zhe Li, Zhi Ye, Yan Xu, Binbin Wang, Congcong Wang, Yunpeng Dai, Jinfeng Lu, Boxun Lu, Wanju Zhang & Yang Li
The RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may preferentially infect neuronal progenitor cells (NPCs) in the developing foetal brain. Ex vivo ZIKV infection induces RNAi-mediated antiviral response in human NPCs, but not in the more differentiated NPCs or somatic...

The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals

Yuqiang Zhang, Zhe Li, Zhi Ye, Yan Xu, Binbin Wang, Congcong Wang, Yunpeng Dai, Jinfeng Lu, Boxun Lu, Wanju Zhang & Yang Li
The RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may preferentially infect neuronal progenitor cells (NPCs) in the developing foetal brain. Ex vivo ZIKV infection induces RNAi-mediated antiviral response in human NPCs, but not in the more differentiated NPCs or somatic...

Integrated analysis of miRNA and mRNA expression profiles in p53-edited PFF cells

Chuanmin Qiao, Weiwei Liu, Haoyun Jiang, Maozhang He, Qiang Yang & Yuyun Xing
p53 is the most frequently mutated gene in human cancers, with over half of all tumors harboring mutation at this locus. R248 and R249 (corresponding to porcine R241 and R242), are among the hotspot mutations frequently mutated in liver, lung, breast, and some other cancers. In this study, p53 gene was knocked out or point-edited (R241 and R242 were converted to 241W and 242S) in porcine fetal fibroblast (PFF) cells via CRISPR-Cas9 technique. High throughput...

Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients

Baoqing Sun, Ying Feng, Xiaoneng Mo, Peiyan Zheng, Qian Wang, Pingchao Li, Ping Peng, Xiaoqing Liu, Zhilong Chen, Huimin Huang, Fan Zhang, Wenting Luo, Xuefeng Niu, Peiyu Hu, Longyu Wang, Hui Peng, Zhifeng Huang, Liqiang Feng, Feng Li, Fuchun Zhang, Fang Li, Nanshan Zhong & Ling Chen
The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and...

Risks and features of secondary infections in severe and critical ill COVID-19 patients

Haocheng Zhang, Yi Zhang, Jing Wu, Yang Li, Xian Zhou, Xin Li, Haili Chen, Mingquan Guo, Shu Chen, Feng Sun, Richeng Mao, Chao Qiu, Zhaoqin Zhu, Jingwen Ai & Wenhong Zhang
Objectives Severe or critical COVID-19 is associated with intensive care unit admission, increased secondary infection rate, and would lead to significant worsened prognosis. Risks and characteristics relating to secondary infections in severe COVID-19 have not been described. Methods Severe and critical COVID-19 patients from Shanghai were included. We collected lower respiratory, urine, catheters, and blood samples according to clinical necessity and culture and mNGS were performed. Clinical and laboratory data were archived. Results We found...

Establishing a high sensitivity detection method for SARS-CoV-2 IgM/IgG and developing a clinical application of this method

Chunyan Zhang, Lei Zhou, Hao Liu, Sibing Zhang, Yaping Tian, Junli Huo, Fei Li, Yao Zhang, Bo Wei, Dan Xu, Jinwei Hu, Jiayi Wang, Yuxuan Cheng, Wenjie Shi, Xiuli Xu, Jianping Zhou, Peipei Sang, Xudong Tan, Weiwei Wang, Minjie Zhang, Bin Wang, Yujun Zhou, Kan Zhang & Kunlun He
COVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG...

Tranilast attenuates neuropathic pain during type-2 diabetes by inhibiting hypoxia-induced pro-inflammatory cytokines in Zucker diabetic fatty rat model

Wei Zhang, Jun Ma, Shan Wang, Tao Huang & Min Xia
The modulatory effect of tranilast on neuropathic pain in type-2 diabetes (T2DM) remains unclear. We monitored interleukin (IL)-1β, nuclear factor-κB (NF-κB) and tumour necrosis factor-α (TNF-α) levels during the progression of T2DM induced neuropathic pain in rats, and assessed the impact of tranilast treatment of increasing concentrations (0, 200 and 400 mg/kg/day via oral gavage in 1% NaHCO3 delivered as 100 mg/kg twice a day) on the levels of cytokine production, as well as on...

The novel outer membrane protein from OprD/Occ family is associated with hypervirulence of carbapenem resistant Acinetobacter baumannii ST2/KL22

Mingxi Hua, Jingyuan Liu, Pengcheng Du, Xinzhe Liu, Min Li, Huizhu Wang, Chen Chen, Xinmin Xu, Yu Jiang, Yajie Wang, Hui Zeng & Ang Li
Acinetobacter baumannii has become a major healthcare threat that causes nosocomial infections, especially in critically ill patients. The spread of carbapenem resistant A. baumannii (CRAB) strains has long been a clinical concern. It is important to study the epidemiology and virulence characteristics of different CRAB isolates in order to tailor infection prevention and antibiotic prescribing. In this study, a total of 71 CRAB isolates were collected in the hospital, and clinical characteristics of infections were...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Thioridazine hydrochloride: an antipsychotic agent that inhibits tumor growth and lung metastasis in triple-negative breast cancer via inducing G0/G1 arrest and apoptosis

Yanlin Song, Lu Li, Jiao Chen, Hongli Chen, Bomiao Cui, Yun Feng, Ping Zhang, Qiangsheng Zhang, Yong Xia & Min Luo
Triple-negative breast cancer (TNBC) is the most aggressive type of breast cancer and still lacks available targeted therapy options. Thioridazine hydrochloride (Thi-hyd), a common phenothiazine antipsychotic drug, has shown great tumor-suppressive potential in several types of cancer cells. In this study, we explored the anti-tumor effects of Thi-hyd on TNBC. Thi-hyd significantly inhibited the viability and migration of TNBC cells in vitro. PI3K/AKT signaling pathway was verified to be related to Thi-hyd-induced tumor inhibition. G0/G1...

Study on influencing factors of Pickering emulsion stabilized by modified montmorillonite and fatty alcohol polyoxyethylene ether

Qian Chen, Zeyu Yang, Xiumei Tai, Yanyun Bai & Guoyong Wang
Pickering emulsion is widely used in food, drug administration, and cosmetics. In practical applications, surfactants are often co-present with particles, so it is particularly critical to study particle-surfactant interactions. Pickering emulsions were prepared using stearyltrimethylammoniumchloride organic montmorillonite (STAC/MMT) and fatty alcohol polyoxyethylene ether (AEO-3) as stabilizers. Effects of AEO-3 content, STAC/MMT content, oil-water ratio, emulsification temperature, emulsification time on emulsion stability were systematically revealed by studying the particle size distribution and phase volume of the...

A new biflavonoids from Aster tataricus induced non-apoptotic cell death in A549 cells

Ting Chen, Peng Yang, Hai-jun Chen & Bin Huang
A new biflavonoids, (2R,2''R)-7-O-methyl-2,3,2'',3''-tetrahydrorobustaflavone (1), along with five known flavonoids (2-6) were isolated from the MeOH extract of Aster tataricus. Among them, compounds 1-2 were the C-3'-C-6'' type biflavonoids obtained from the genus Aster for the first time. The structures and absolute configurations of compound 1 was confirmed based on extensive spectroscopic and circular dichroism analyses. Compound 1 exhibited moderate cytotoxicity against seven human cancer A549, HepG2, PC3, DU145, MCF-7, LOVO and NCI-H1975 cell lines....

Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking

Wei Hou, Wenjie Kang, Yangyang Li, Yanke Shan, Shouyu Wang & Fei Liu
It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses. Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded RNA virus of the Coronavirus family, which can infect swine of all ages and cause severe economic losses in the swine industry. Elucidating the molecular mechanisms of the intercellular transport of PEDV through microtubule, dynein and kinesin-1 will be...

Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking

Wei Hou, Wenjie Kang, Yangyang Li, Yanke Shan, Shouyu Wang & Fei Liu
It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses. Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded RNA virus of the Coronavirus family, which can infect swine of all ages and cause severe economic losses in the swine industry. Elucidating the molecular mechanisms of the intercellular transport of PEDV through microtubule, dynein and kinesin-1 will be...

Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-κB and MAPKs signaling pathways

Aimin Liu, Buxin Zhang, Wei Zhao, Yuanhui Tu, Qingxing Wang & Jing Li
Psoriasis is a chronic inflammatory skin disease that affects approximately 2% of worldwide population, and causing long-term troubles to the patients. Therefore, it is urgent to develop safe and effective therapeutic drugs. Catalpol is a natural iridoid glucoside, that has several remarkable pharmacological effects, however, whether catalpol can alleviated psoriasis has not been explored. The goal of the present work is to study the role of catalpol in psoriasis in vivo and in vitro. Imiquimod-induced...

A new functionalized reduced graphene oxide adsorbent for removing heavy metal ions in water via coordination and ion exchange

Chao-Zhi Zhang, Bin Chen, Yuan Bai & Jing Xie
Heavy metal ion pollution has become a serious problem. In this paper, a new type of adsorbent, reduced graphene oxide grafted by 4-sulfophenylazo groups (RGOS), was synthesized to adsorb heavy metal ions in an aqueous solution via two kinds of adsorption modes, ion exchange and coordination. The maximum adsorption capacities of the RGOS for Pb(II), Cu(II), Ni(II), Cd(II) and Cr(III) were 689, 59, 66, 267 and 191 mg/g, respectively. Adsorption equilibrium time of RGOS for...

Association study of hypertension susceptibility genes ITGA9, MOV10, and CACNB2 with preeclampsia in Chinese Han population

Qian Xin, Gang Xin, Li Li, Wenjuan Sun, Wen Jiang, Jue Wang, Yun Luan, Ying Zhang, Ling Cheng, Shuhong Duan, Fanzhen Hong, Qinghong Ji & Weihong Ma
Preeclampsia (PE) is a disorder that occurs during the pregnancy and could affect the maternal and perinatal mortality as well as morbidity. The aim of our study is to investigate the associations between the hypertension susceptibility genes ITGA9, MOV10 and CACNB2 with PE in Chinese Han population. A case–control study including 178 PE patients and 202 healthy controls was conducted to assess the associations between three loci (ITGA9 rs155524, MOV10 rs2932538 and CACNB2 rs4373814) and...

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