4,942 Works

A temperate Siphoviridae bacteriophage isolate from Siberian tiger enhances the virulence of methicillin-resistant Staphylococcus aureus through distinct mechanisms

Dan Yang, Shuang Wang, Erchao Sun, Yibao Chen, Lin Hua, Xiangru Wang, Rui Zhou, Huanchun Chen, Zhong Peng & Bin Wu
The emergence and worldwide spread of Methicillin-resistant Staphylococcus aureus (MRSA) pose a threat to human health. While bacteriophages are recognized as an effective alternative to treat infections caused by drug resistant pathogens, some bacteriophages in particular the temperate bacteriophage may also influence the virulence of the host bacteria in distinct ways. In this study, we isolated a bacteriophage vB_Saus_PHB21 from an epidermal sample of Siberian tiger (Panthera tigris altaica) using an MRSA strain SA14 as...

Implementation of novel boolean logic gates for IMPLICATION and XOR functions using riboregulators

Chaoxin Chen, Qi Wu, Qingying Ke, Ting Wang, Yifan Zhang, Feiwen Wei, Xiaolong Wang & Guanglei Liu
To date, several different types of synthetic genetic switches, including riboregulators, riboswitches, and toehold switches, have been developed to construct AND, OR, NOT, NAND, NOR, and NOT IMPLICATION (NIMP) gates. The logic gate can integrate multiple input signals following a set of algorithms and generate a response only if strictly defined conditions are met. However, there are still some logic gates that have not been implemented but are necessary to build complex genetic circuits. Here,...

Role of lncRNA LINC01194 in hepatocellular carcinoma via the miR-655-3p/SMAD family member 5 axis

Yang Liu, Jie Liu, Junkai Cui, Ruolei Zhong & Guoyang Sun
Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Long non-coding RNA terminal differentiation-induced non-coding RNA regulates cisplatin resistance of choroidal melanoma by positively modulating extracellular signal-regulated kinase 2 via sponging microRNA-19b-3p

Wei Li, Aiqin Nie, Longyu Jin, Yubo Cui, Ning Xie & Gaohua Liang
In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

Metabolomics research on the effect of dexamethasone‐induced chronic stress in serum of rabbits

Hongli Liu, Bin Zhang, Lei Liu & Fuchang Li
Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Metabolomics research on the effect of dexamethasone‐induced chronic stress in serum of rabbits

Hongli Liu, Bin Zhang, Lei Liu & Fuchang Li
Stress is a common factor that reduces production performance. However, the physiology of stress is not fully understood. In this study, 40-day-old rabbits were injected 1.0 mg/kg BW Dexamethasone to induce stress. A metabolomic study on serum of stress-induced rabbits was performed to research the metabolism mechanism of stress by using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS). Clear separations between stress rabbits and control rabbits were observed by principal component analysis (PCA) based...

Nutritional components and protein quality analysis of genetically modified phytase maize

Yichun Hu, Liqin Linghu, Min Li, Deqian Mao, Yu Zhang, Xiaoguang Yang & Lichen Yang
The nutritional components and protein quality of genetically modified maize expressing phytase gene (GM) were analyzed and evaluated in this study. The nutritional components were analyzed by Chinese national standard methods. The ileostomy Bama miniature pigs were utilized to analyze the true digestibility of protein and amino acids. The digestible indispensable amino acid score (DIAAS) was adopted to evaluate the protein quality of GM, its parental maize (PM) and commercial available maize Zhengdan 958 (ZD)....

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Aetiology of severe community acquired pneumonia in adults identified by combined detection methods: a multi-centre prospective study in China

Jieming Qu, Jing Zhang, Yu Chen, Yi Huang, Yusang Xie, Min Zhou, Yuping Li, Dongwei Shi, Jinfu Xu, Qiuyue Wang, Bei He, Ning Shen, Bin Cao, Danyang She, Yi Shi, Xin Su, Hua Zhou, Hong Fan, Feng Ye, Qiao Zhang, Xinlun Tian & Guoxiang Lai
Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Morphology and phylogeny of two new Thalassiosiroid taxa (Bacillariophyceae), with two marginal rimoportulae

Biaobiao Niu, Yaqiong Guo, Nina Lundholm & Yang Li
Thalassiosira and related taxa i.e. the taxa within Thalassiosirales are among the most abundant and widespread diatoms. The vast majority have one marginal rimoportula, but very few always have two. The knowledge on Thalassiosiroid taxa in Chinese waters is restricted, and to explore the diversity, monoclonal strains were established from southern Chinese coastal waters. Morphological data were based on light and scanning electron microscopy. The D1‒D3 region of the ribosomal large subunit (LSU) and the...

Difficulties translating antisense-mediated activation of Frataxin expression from cell culture to mice

Audrius Kilikevicius, Jun Wang, Xiulong Shen, Frank Rigo, Thahza P. Prakash, Marek Napierala & David R. Corey
Friedreich’s ataxia (FA) is an inherited neurodegenerative disorder caused by decreased expression of frataxin (FXN) protein. Previous studies have shown that antisense oligonucleotides (ASOs) and single-stranded silencing RNAs can be used to increase expression of frataxin in cultured patient-derived cells. In this study, we investigate the potential for oligonucleotides to increase frataxin expression in a mouse model for FA. After confirming successful in vivo delivery of oligonucleotides using a benchmark gapmer targeting the nuclear noncoding...

Stachydrine is effective and selective against blast phase chronic myeloid leukaemia through inhibition of multiple receptor tyrosine kinases

Ruixin Gu, Wei Zhang & Dandan Xu
Resistance to BCR-ABL tyrosine kinase inhibitor (TKI) is the cause of treatment failure in blast phase chronic myeloid leukaemia (BP-CML). Agents that act synergistically with BCR-ABL TKI are required to improve response. This work investigated the effects of stachydrine in CML. CML cells were treated with control or stachydrine at 20, 40 and 80 µM. Proliferation and apoptosis were examined after 72 h treatment. Combination studies were performed in four groups: control, TKI, stachydrine and...

Abietane diterpenoids with neuroprotective activities from Phlegmariurus carinatus

Yang Liu, Qiang Wang, Dong-Kun Zheng, De Zhang, Zhen Xie, Jia-Wei Hu, Xin-Hua Xie, Jing Li & Shu-Ping Jiang
Two new abietane diterpenoids, phlecarinatone A (1) and phlecarinatone B (2), along with two known analogues (3 and 4), were isolated from Phlegmariurus carinatus. The structures of 1 − 4 were unambiguously elucidated by comprehensive spectroscopic analyses. Abietane diterpenoids were isolated from the plant for the first time. All isolates were tested for their neuroprotective activities against H2O2-induced SH-SY5Y cells injury, and compound 2 showed moderate effect at the concentrations ranging from 5 ∼ 20...

Two QTL for kernel number per spike identified from durum wheat

Jingtian Yang, Zhengqiao Liao, Baoguo Du, Kuan Zhang & Lei Liu
As one of the three yield components, kernel number per spike (KNPS) plays a key role in determining wheat yield. In this study, in combination with the previously constructed genetic map for a tetraploid wheat recombinant inbred line (RIL) population developed from the cross between a durum wheat and a wild emmer wheat and phenotype data from four environments, two major and stably expressed quantitative trait loci (QTL) for KNPS were identified. The phenotypic variation...

Comparative phytochemical property and antioxidant activity of sixteen Mei (Prunus mume) fruit cultivars from six provinces in China

Zhihua Liu, Yezi Yang, Chongzhi Sun, Yang Wu & Xiaobo Li
Mei (Prunus mume) is widely planted in Eastern Asian countries. To evaluate the effect of cultivar and producing origin on the quality of Mei fruit, we studied the phytochemical properties and antioxidant activities of the 16 Mei fruit cultivars from 6 provinces in China, as well as their profiles of 121 volatile compounds, 5 organic acids, 13 minerals, 24 amino acids and 3 active peptides. Multivariate analysis revealed that the four Mei fruit cultivars with...

RETRACTED ARTICLE: Long noncoding RNA H19 regulates HIF-1α/AXL signaling through inhibiting miR-20b-5p in endometrial cancer

He Zhu, Yue-Mei Jin, Xue-Man Lyu, Li-Mei Fan & Fei Wu
We, the Editors and Publisher of the journal Cell Cycle, have retracted the following article: He Zhu, Yue-Mei Jin, Xue-Man Lyu, Li-Mei Fan & Fei Wu (2019) Long noncoding RNA H19 regulates HIF-1α/AXL signaling through inhibiting miR-20b-5p in endometrial cancer, Cell Cycle, 18:19, 2454-2464, DOI: 10.1080/15384101.2019.1648958 Since publication, the authors have found significant errors in the statistical analysis of the research reported in this article. As this error directly impacts the accuracy of the results...

Integration of transcriptomics and metabolomics to identify key coumarin biosynthetic genes in Bupleurum chinense

Bin Yang, Shi-Hang Liu, Yilian He, Yuchan Li, Liang Feng, Meng Zhang, Jun Zhao, Yiguan Zhang, Xia Yu, Hua Chen, Dabin Hou, Junning Zhao & Ma Yu
Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in...

Late paleozoic–mesozoic subduction and accretion of the paleo-pacific plate: insights from the ocean plate stratigraphy of the wandashan accretionary complex, NE China

Yong Liang, Han Zheng, Huan Li, Michael W. Förster & Zhihan Li
The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

Late paleozoic–mesozoic subduction and accretion of the paleo-pacific plate: insights from the ocean plate stratigraphy of the wandashan accretionary complex, NE China

Yong Liang, Han Zheng, Huan Li, Michael W. Förster & Zhihan Li
The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Rui Zhou, Menglong Fan, Mei Zhao, Xinqiang Jiang & Qinghua Liu
Leaves are the main vegetative organs of the aboveground part of plants and play an important role in plant morphogenesis. KNOTTED-LIKE HOMEOBOX (KNOX) plays a crucial role in regulating leaf cell fate and maintaining leaf development. In this study, we analyzed LtKNOX1 from Lilium tsingtauense and illustrated its function in transgenic plants. Tissue-specific expression analysis indicated that LtKNOX1 was highly expressed in stems, young flower buds, and shoot apical meristems (SAMs). Ectopic overexpression of LtKNOX1...

Controlled synthesis of cube-like ZnSnO3 decorated by nickel-based films and electrochemical applications on lithium-sulfur batteries

Mianjiao Jiang, Jun Wu, Hongmei Guo, Hongfang Zhang, Manli Zhu & Xiaobang Xu
Transition metal oxide was considered as an effective cathode additive to depress ‘shuttle effect’ in lithium-sulfur (Li-S) batteries via forming a strong polar chemical bond with polysulfides. Here, we firstly synthesized unique S@Ni/NiO/ZnSnO3 microcubes as composite cathodes for Li-S batteries, in which Ni/NiO films were deposited onto surface of as-prepared ZnSnO3 microcubes via a hydrothermal reaction and later calcinations process. The S@Ni/NiO/ZnSnO3 composite showed attractive electrochemical performances with a high initial discharge-specific capacity of 1070...

Design, synthesis and nematocidal activity of novel 1,2,4-oxadiazole derivatives with a 1,3,4-thiadiazole amide moiety

Dan Liu, Zhengxing Wang, Jing-Jiang Zhou & Xiuhai Gan
A series of novel 1,2,4-oxadiazole derivatives containing 1,3,4-thiadiazole amide group was synthesized and their nematocidal activities were evaluated. The results indicated that compounds 4-methoxy-N-(5-(((3-(p-tolyl)-1,2,4-oxadiazol-5-yl)methyl)thio)-1,3,4-thiadiazol-2-yl)benzamide 4i and N-(5-(((3-(4-chlorophenyl)-1,2,4-oxadiazol-5-yl)methyl)thio)-1,3,4-thiadiazol-2-yl)benzamilde 4p showed good nematocidal activity against Bursaphelenchus xylophilus. The corrected mortality of compounds 4i and 4p at 200 mg/L was 57.1% and 60.1%, respectively, which are much better than 13.5% of the commercial seed coating agent Tioxazafen. Compounds 4i and 4p dramatically decreased the head swinging, body fluctuation,...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqing Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Human umbilical cord mesenchymal stem cells contribute to the reconstruction of bladder function after acute spinal cord injury via p38 mitogen-activated protein kinase/nuclear factor-kappa B pathway

Jue Li, Jiliang Huang, Ling Chen, Wei Ren & Wenzhi Cai
The association between spinal cord injury (SCI) and bladder symptoms has been intensively described. Human umbilical cord mesenchymal stem cell (hUC-MSC) treatment is beneficial to the recovery of bladder function after SCI, but its mechanism is unclear. We established an SCI model, and prepared hUC-MSCs in advance, followed by verification using flow cytometry. The Basso, Beattie and Bresnahan (BBB) score and urodynamic index were employed to evaluate motor function and bladder functions, respectively. Hematoxylin–eosin staining,...

Up-regulation of caveolin 1 mediated by chitosan activates Wnt/ β-catenin pathway in chronic refractory wound diabetic rat model

Jie Gao, Jiayuan Zhang, Lianheng Xia, Xuewei Liang, Wukun Ding, Meiyu Song, Linggen Li & Zhen Jia
Diabetes mellitus (DM) can be implicated in the perturbations of vascular integrity and the dysfunction of angiogenesis. Chitosan has the advantage of promoting the vascular endothelial cell proliferation. However, the molecular mechanism of action in the promotion of wound healing by chitosan derivatives is still debated. In the current study, DM with chronic wound (CW) model rats were prepared and treated with chitosan. Vascular endothelial cells isolated from granulation tissues were conducted by RNA sequencing....

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