Special electromagnetic field-treated water (SEW) and far-infrared radiation (FIR) can reduce acute respiratory distress syndrome (ARDS) in rats inflicted by lipopolysaccharides (LPSs). However, little is known about its underlying molecular mechanism. Differentially expressed proteins (DEPs) of SEW and FIR interventions were obtained from a proteomics database. A total of 89 DEPs were identified. Enrichment analysis of DEPs was performed using the Database for Annotation, Visualization, and Integrated Discovery. These DEPs were associated with the responses...

This study explored the association of exposure to air pollutants during gestational and the first year of life with physical growth in preschoolers. The linear and logistic regression models were used to estimate the associations between air pollution and childhood growth. After adjusting for confounders, z-scores of body mass index (zBMI, and 95% confidence intervals, 95%CI) increased by 1.164(1.054,1.285), 1.136(1.050,1.228) and 1.165(1.041,1.303), associated with per interquartile range (IQR) increase in NO2 (nitrogen dioxide), PM2.5 and...

With the worldwide prevalence of diabetes and considering the complicated microenvironment of diabetic wounds, the design and development of innovative multifunctional wound dressing materials are much wanted for the treatment of hard-to-heal wounds in diabetic patients. In the present study, anti-inflammatory ingredients loaded with nanofibrous wound dressing materials were manufactured by a promising blend-electrospinning strategy, and their capability for treating the diabetic wound was also systematically explored. A polymer blend consisting of Chitosan (CS) and...

Abstract Background Ovarian aging is a process of decline in its reserve leading to ovary dysfunction and even reduced health quality in offspring. However, aging-related molecular pathways in the ovary remain obscure. Lysine succinylation (Ksuc), a newly post-translational modification (PTM), has been found to be broadly conserved in both eukaryotic and prokaryotic cells, and associated with multiple pathophysiological processes. There are no relevant reports revealing a link between the molecular mechanisms of ovarian aging and...

Additional file 1: Figure S1. Validation of successful mouse POI model construction. (A) and (B) Histopathological examination and follicle counts of ovaries. Scale bar: 200 μm. (C) and (D) Ovary weight (mg) and ovary index (ovary weight / body weight, mg/g). (E) and (F) Levels of serum AMH and E2 concentrations. * P < 0.05, ** P < 0.01.

Additional file 2: Figure S2. (A) The negative control of IHC-Ksuc and -Cleaved Caspase 3 in mouse ovary administered in vivo sections. Scale bar: 100 μm (B) The negative control of IF-Ksuc (green) and -DAPI (blue) in mouse ovarian sections. Scale bar: 100 μm.

Additional file 7.

Abstract Background English as a Media of Instruction (EMI) teacher development project is based upon the framework for teacher Continuing Professional Development (CPD) and aims to effectively improve both the confidence and overall capacity of EMI lecturers. Kunming Medical University(KMU) conducted the EMI training project to improve teachers’ competence for MBBS education. This study aimed to assess teachers’ changes following the implementation of this training project, via the Kirkpatrick evaluation model. Methods A total of...

Supplemental material, sj-xlsx-1-cat-10.1177_10760296231167849 for Low MHR Is Associated with Hemorrhagic Transformation in Acute Large Artery Atherosclerosis Ischemic Stroke Patients with Intravenous Thrombolysis by Delong Meng, MM, Yan Li, MM, Ting Ju, MM, Wei Huo, MD, and Mingfei Wang, MD in Clinical and Applied Thrombosis/Hemostasis

Additional file 11: Fig. S11. Multi-omics subtypes of PDAC patients, related to Fig. 6. A Consensus matrices of identified clusters (k = 2 to 4) of transcriptomic, proteomic, and phosphoproteomic subtypes. B The consensus CDF and delta area (change in CDF area) plots, as well as the silhouette plots, are shown. C Heatmaps showing the comparison between transcriptomic and phosphoproteomic subtypes (columns) with proteomic subtypes (rows). Each row sums to one, with different entries showing...

Additional file 13: Fig. S13. Proteomic subtypes divided early-stage PDAC patients into subtypes with different prognosis, related to Fig. 6. A Summary of the relationship between proteomic subtypes and TNM stage subtypes. B Kaplan-Meier curves for overall survival of early-stage (IA and IB) PDAC patients based on 3 proteomic subtypes (log-rank test). C Bar plot indicating the enriched pathways in early-stage patients who were divided into three proteomic subtypes. D Heatmap of differentially expressed kinase...

Rapid coherent Raman hyperspectral imaging shows great promise for applications in sensing, medical diagnostics and metabolism dynamic monitoring. However, the spectral acquisition speed of current multiplex CARS microscopy is generally limited by the spectrometer integration time, making it difficult to achieve the video frame rate imaging. On the other hand, as the detection speed increases, the signal-to-noise ratio (SNR) of single spectrum will decrease leading to a terrible imaging quality. In this letter, we report...

Additional file 21: Fig. S21. Protein expression changes in PANC-1 cell lines after HOGA1 knocked down and over expressed, related to Fig. 8. A The schematic work flow of validation experiments for the protein expression changes after HOGA1 knocked down and over expressed. B The volcano plot showing the proteins that significantly altered between PANC-1 cells with HOGA1 KD (left)/HOGA1 OE (right) and WT. The GO processes enriched by proteins upregulated in HOGA1 KD cell...

Additional file 22: Fig. S22. Hematoxylin and eosin (H&E) staining on PDACs. The tumor cell purities of tumor tissues and the non-tumor cell purities of tumor-adjacent tissues.

Additional file 25: Table S3. Integrated Multi-omics Features in Tumor Tissues Compared with NATs of the PDAC. Table S3A Matrix of 7,055 protein groups and their relative intensities in at least 1/6 of the samples (226 tumors and 220 NATs). Table S3B 19,992 mRNA and their ratio of tumors/NATs (54 tumors and 51 NATs). Table S3C A list of protein identified both in protein and mRNA level. Table S3D A list of phosphosites used for...

Additional file 25: Table S3. Integrated Multi-omics Features in Tumor Tissues Compared with NATs of the PDAC. Table S3A Matrix of 7,055 protein groups and their relative intensities in at least 1/6 of the samples (226 tumors and 220 NATs). Table S3B 19,992 mRNA and their ratio of tumors/NATs (54 tumors and 51 NATs). Table S3C A list of protein identified both in protein and mRNA level. Table S3D A list of phosphosites used for...

Additional file 28: Table S6. Proteomic Subtypes of PDAC Patients. Table S6A Proteomic subtypes, transcriptomic subtypes and phosphoproteomic subtypes of PDAC patients. Table S6B A list of differentially expressed proteins among three subtypes. Table S6C Pathway alterations in the proteomic subtypes of the PDAC cohort. Table S6D Matrix of protein expression and kinase activity of kinase activated in S-III. Table S6E Matrix of differential pathway enrichment between patients located in H-PDAC and BT-PDAC. Table S6F...

Additional file 2: Fig. S2. Phosphoproteomic characterization, related to Fig. 1. A. MS2 spectrums of the YAP1 at S109 and RB1 at S807. B. IHC staining of YAP1 at S109 and RB1 at S807 in PDAC tumor tissues and NATs. C. Boxplot indicting the phosphosite expression of YAP1_pS109 and RB1_pS807 between tumors and NATs.

Additional file 3: Fig. S3. The impacts of somatic copy number alterations in PDAC cohort, related to Fig. 2. A Bar plots showing pathway enrichment of cis/trans-effect between CNA and protein (left) or phosphoprotein (right). B Distribution of IRF6 status between the younger patients’ group (≤ 60) and the older patients’ group (> 60) (left). The boxplot reveals the comparison of the mRNA (middle) and protein (right) abundance of IRF6 between the younger patients (≤...

Additional file 8: Fig. S8. The effects of diabetes on the proteogenomic characteristics of PDAC, related to Fig. 4. A The boxplot representing the comparison of blood glucose concentration between patients with diabetes and without diabetes. B The Kaplan-Meier curves for overall survival based on diabetic patients with or without medical treatment. C The heatmap showing the distribution of 10q25.3 deletion and MTOR mutations across different samples in TCGA cohort. D The bar plot showing...

The Supporting Information for deep learning part

Herein, a new series of 2-chloro-N-(5-(2-oxoindolin-3-yl)-4H-pyrazol-3-yl) acetamide derivatives containing 1,3,4-thiadiazole (10a–i) and 4H-1,2,4-triazol-4-amine (11a–r) moiety was designed, synthesised as novel anticancer agents. The antiproliferative activity values indicated that compound 10 b stood as the most potent derivative with IC50 values of 12.0 nM and 10 nM against A549 and K562 cells, respectively. Mechanism investigation and docking studies of 10 b indicated that it possessed good apoptosis characteristic and dose-dependent growth arrest of A549 and K562...

Organoids, the 3D culture systems derived from stem cells, are promising models for human organs. But organoid study requires large-volume imaging with single cell resolution, which is beyond the spatial bandwidth limit of conventional optical microscopy. Herein, we propose a lensless holographic microscope empowered with a time and memory-saving algorithm. It solves the tradeoff between the imaging field of view, resolution and processing speed, and provides a practical tools for organoids’ study. We first build...

Learning-based computer-generated holography (CGH) algorithms appear as novel alternatives to generate phase-only holograms. However, most existing learning-based approaches underperform their iterative peers in terms of display quality. Here, we propose an unsupervised Fourier-inspired neural network that could generate phase-only holograms in real time and obtain high-fidelity reconstructed images. Both simulation and experiment were performed to showcase its capability. By explicitly leveraging Fourier transforms within the neural network architecture, global features of the holograms are more...

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials