Autophagy induced by Vip3Aa has a pro-survival role in Spodoptera frugiperda Sf9 cellsXiaoyue Hou, Lu Han, Baoju An & Jun Cai
Vip3Aa is an insecticidal protein that can effectively control certain lepidopteran pests and has been used widely in biological control. However, the mechanism of action of Vip3Aa is unclear. In the present study, we showed that Vip3Aa could cause autophagy in Sf9 cells, which was confirmed by the increased numbers of GFP-Atg8 puncta, the appearance of autophagic vacuoles, and an elevated Atg8-II protein level. Moreover, we found that the AMPK-mTOR-ULK1 pathway is involved in Vip3Aa-induced...
Preparation, Characterization of Carboxyl Polyaldehyde Sugars and Application as Innovative Anti-crease Finishing Agents for Cotton FabricJiangfei Lou, Dan Wang & Xuerong Fan
We have designed and prepared four new cellulose cross-linking agents using NaClO/NaBr/TEMPO and sodium periodate selective catalytic oxidation systems. First, the primary hydroxyl group of sucrose, trehalose, maltose, or lactose was carboxylated through NaClO/NaBr/TEMPO system, and then sodium periodate was used to selectively oxidize the carboxyl compound to obtain carboxyl polyaldehyde sugars. They contained multiple aldehyde groups and carboxyl groups in the molecule, had high reactivity, and can be covalently cross-linked with cellulose. To evaluate...
Is SARS-CoV-2 originated from laboratory? A rebuttal to the claim of formation via laboratory recombinationPei Hao, Wu Zhong, Shiyang Song, Shiyong Fan & Xuan Li
Is SARS-CoV-2 originated from laboratory? A rebuttal to the claim of formation via laboratory recombination
MustSeq, an alternative approach for multiplexible strand-specific 3’ end sequencing of mRNA transcriptome confers high efficiency and practicalityLiyao Mai, Yinbin Qiu, Zhiwei Lian, Caiming Chen, Linlin Wang, Yao Yin, Siqi Wang, Xiang Yang, Yazi Li, Wanwan Peng, Chaochao Luo & Xinghua Pan
RNA-seq has been widely used to reveal the molecular mechanism of variants of life process. We have developed an alternative method, MustSeq, which generates multiple second strands along a single 1st strand cDNA by random-priming initiation, immediately after reverse transcription for each RNA extract using sample-barcoded poly-dT primers, then 3ʹ ends-enriching PCR is applied to construct the library. Unlike the conventional RNA seq, MustSeq avoids procedures such as mRNA isolation, fragmentation and RNA 5ʹ-end capture,...
Additional file 3 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditionsMenglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 3: Table S2. Expression of wheat new miRNAs in crown tissues.
Additional file 4 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditionsMenglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 4: Table S3. Information of wheat_miRNA2693 and wheat_miR5099.
Additional file 8 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditionsMenglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 8: Table S5. Sequence of primers used in this study.
CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progressionXuan Li, Kai-Bin Yang, Wei Chen, Jia Mai, Xiao-Qi Wu, Ting Sun, Rui-Yan Wu, Lin Jiao, Dan-Dan Li, Jiao Ji, Hai-Liang Zhang, Yan Yu, Yu-Hong Chen, Gong-Kan Feng, Rong Deng, Jun-Dong Li & Xiao-Feng Zhu
Macroautophagy/autophagy plays an important role during the development of human cancer. BECN1 (beclin 1), a core player in autophagy regulation, is downregulated in many kinds of malignancy. The underlying mechanism, however, has not been fully illuminated. Here, we found that CUL3 (cullin 3), an E3 ubiquitin ligase, could interact with BECN1 and promote the K48-linked ubiquitination and degradation of this protein; In addition, CUL3 led to a decrease in autophagic activity through downregulating BECN1. We...
Additional file 2 of Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trialJie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Additional file 2.
Additional file 3 of Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trialJie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Additional file 3.
Alleviation of refractory IgA nephropathy by intensive fecal microbiota transplantation: the first case reportsJin Zhao, Ming Bai, Xiaoxia Yang, Yan Wang, Rong Li & Shiren Sun
Gut dysbiosis may be implicated in the pathogenesis of IgA nephropathy (IgAN) through immune and/or metabolite pathways. Fecal microbiota transplantation (FMT) could reestablish the micro-ecological balance in IgAN, although this has never been attempted before. We explored whether FMT could be efficacious in treating IgAN in two patients with refractory IgAN. Two Chinese female patients with IgAN failed to achieve clinical remission after receiving several rounds of immunosuppressive therapy and suffered from unbearable adverse effects...
Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAKXuelin Han, Xueting Su, Zhiqian Li, Yanxi Liu, Shuo Wang, Miao Zhu, Changjian Zhang, Fan Yang, Jingya Zhao, Xianping Li, Fangyan Chen & Li Han
Complement receptor 3 (CD11b/CD18) is an important receptor that mediates adhesion, phagocytosis and chemotaxis in various immunocytes. The conidia of the medically-important pathogenic fungus, Aspergillus fumigatus can be internalized into alveolar epithelial cells to disseminate its infection in immunocompromised host; however, the role of CR3 in this process is poorly understood. In the present study, we investigated the potential role of CR3 on A. fumigatus internalization into type II alveolar epithelial cells and its effect...
POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysisJinze Meng, Hao Wen, Xintong Li, Boyang Luan, Shiqiang Gong, Jie Wen, Yifei Wang & Lei Wang
Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study aimed to identify potential genes for AAA growth and expansion based on comprehensive bioinformatics approaches. Firstly, 29 up-regulated genes were identified through DEGs analysis between large AAA and small AAA in GSE57691. Secondly,...
Absent in melanoma 1-like (AIM1L) serves as a novel candidate for overall survival in hepatocellular carcinomaWenliang Zhou, Yuan Zhang, Shixi Zhang & Zongguo Yang
Identifying biomarkers for hepatocellular carcinoma (HCC) survival is of great importance for the early detection, monitoring, and predicting for prognosis. This study aimed to investigate the candidate biomarkers for predicting overall survival (OS) in HCC patients. Using RTCGAToolbox, top 50 upregulated differential expressed genes (DEGs) were identified. The least absolute shrinkage and selection operator (LASSO) and Cox models were used to select powerful candidate genes, and log rank method was used to address the survivor...
Synthesis and properties of novel betaine fluorocarbon surfactantsWen Ren, Shuixiang Xie, Yuanpeng Cheng, Mingdong Zhang, Chunyang Wang, Jialuo Rong & Zongkun Li
In this study, a series of new betaine fluorocarbon surfactants (abbreviated as FSC-m) were synthesized with N,N-dimethyl-1,3-propane diamine, perfluoroalkyl sulfonyl fluoride, and sodium chloroacetate. The novel surfactants were characterized by FI-IR, 1H-NMR and 19F-NMR. The surface activity of these surfactants at the air-water interface was investigated. The oil-water interfacial tension, adsorption, and wettability were studied on the oily drilling cuttings. The results showed that the novel surfactants have excellent surface and interfacial activities and low...
Chitosan/Lemon Residues Activated Carbon Efficiently Removal of Acid Red 18 from Aqueous Solutions: Batch Study, Isotherm, KineticsYongyao Xu, Wangqing Shen, Yin Liu & Jiafeng Wei
In this research, chitosan-decorated activated carbon(AC) was proposed. The AC was cross-linked by glutaraldehyde to prepare adsorbent(AC-CS).The AC-CS has a rough surface. Adding the AC-CS directly to the dye solution can achieve simple and convenient removal of anionic azo dyes acid red 18 (AR-18). In dye solution, the AC-CS was used as adsorbent. The effects of pH, contact time, temperature, initial concentration of AR-18, and the AC-CS dosage on the adsorption efficiency were investigated. Full...
Chitosan/lemon residues activated carbon efficiently removal of acid red 18 from aqueous solutions: batch study, isotherm and kineticsYongyao Xu, Wangqing Shen, Yin Liu & Jiafeng Wei
In this research, chitosan-decorated activated carbon (AC-CS) was proposed. The AC was cross-linked with glutaraldehyde to prepare an adsorbent (AC-CS). The AC-CS has a rough surface. Adding the AC-CS directly to the dye solution can achieve simple and convenient removal of anionic azo dyes acid red 18 (AR-18). In the dye solution, the AC-CS was used as an adsorbent. The effects of pH, contact time, temperature, initial concentration of AR-18 and the AC-CS dosage on...
Cholesterol depletion sensitizes gallbladder cancer to cisplatin by impairing DNA damage responseYonglong Zhang, Yanfeng Liu, Jinlin Duan, Hui Wang, Yuchen Zhang, Ke Qiao & Jian Wang
Gallbladder cancer (GBC) is the common malignancy of the bile tract system with extremely poor clinical outcomes, owing to its metastatic property and intrinsic resistance to the first-line drugs. Although it is well-established that cholesterol abnormity contributes to gallstone formation, a leading risk factor for GBC, the link of cholesterol homeostasis with GBC has not been investigated. The present study systematically examined the genes implicated in cholesterol homeostasis, and revealed altered gene expressions of de...
Excitonic coupling interactions in the self-assembly of perylene-bridged bis(β-cyclodextrin)s and porphyrinKe-Rang Wang, Dong-Sheng Guo, Bang-Ping Jiang & Yu Liu
A supramolecular self-assembly has been constructed by perylene-bridged bis(β-cyclodextrin)s with water-soluble porphyrin through hydrophobic interactions, showing strong excitonic coupling interactions between perylene backbones and included porphyrins.
Molecular recognition of amphiphilic p-sulfonatocalixarene with organic ammoniumsXin-Yue Hu, Shu Peng, Dong-Sheng Guo, Fei Ding & Yu Liu
(2015). Molecular recognition of amphiphilic p-sulfonatocalixarene with organic ammoniums. Supramolecular Chemistry: Vol. 27, Ninth International Symposium of Macrocyclic and Supramolecular Chemistry (ISMSC-9), pp. 336-345.
Molecular Aggregation Behavior of Perylene-Bridged Bis(β-cyclodextrin) and Its Electronic Interactions upon Selective Binding with Aromatic GuestsDong-Sheng Guo, Yu Liu, Ke-Rang Wang, Bang-Ping Jiang & Zhan-Hu Sun
A novel water-soluble perylene bisimide derivative 1 was synthesized with two permethyl-β-cyclodextrin grafts at the imide nitrogens, and its structure was identified by NMR, Fourier transform infrared spectroscopy (FT-IR), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), and elemental analysis. Its aggregation behavior in water and organic solutions was further investigated by UV−vis, fluorescence, and 1H NMR spectra, showing that 1 exhibits strong π···π aggregation in water. Especially, the aggregation and optical properties were comprehensively compared...
Highly Effective Binding of Methyl Viologen Dication and Its Radical Cation by p-Sulfonatocalix[4,5]arenesDong-Sheng Guo, Li-Hua Wang & Yu Liu
The binding behaviors and thermodynamic origins of p-sulfonatocalixarene (C4AS) and p-sulfonatocalixarene (C5AS) with methyl viologen (MV2+) have been investigated by the methods of isothermal titration calorimetry, NMR, and cyclic voltammetry, showing that the binding abilities of C4AS and C5AS and their host selectivity are dramatically pH-controlled, which is closely discussed from the viewpoint of thermodynamics. Moreover, the radical form of MV+• can also be effectively included by C4AS and C5AS.
Molecular recognition of sulfonatocalixarene with organic cations at the self-assembled interface: a thermodynamic investigationYu-Chen Pan, Han-Wen Tian, Shu Peng, Xin-Yue Hu & Dong-Sheng Guo
[\"Complexation of a guanidinium-modified calixarene with diverse dyes and investigation of the corresponding photophysical response\"]Yu-Ying Wang, Yong Kong, Zhe Zheng, Wen-Chao Geng, Zi-Yi Zhao, Hongwei Sun & Dong-Sheng Guo
We herein describe the comprehensive investigation of the complexation behavior of a guanidinium-modified calixarene pentaisohexyl ether (GC5A) with a variety of typical luminescent dyes. Fluorescein, eosin Y, rose bengal, tetraphenylporphine sulfonate and sulfonated aluminum phthalocyanine were employed as classical aggregation-induced quenching dyes. 2-(p-Toluidinyl)naphthalene-6-sulfonic acid and 1-anilinonaphthalene-8-sulfonic acid were selected as representatives of intramolecular charge-transfer dyes. Phosphated tetraphenylethylene was involved as the classical aggregation-induced emission dye. Sulfonated acedan representing one example of two-photon fluorescent probes, was...
Assembly-enhanced molecular recognition of calixareneHan-Wen Tian, Yu-Chen Pan & Dong-Sheng Guo
(2018). Assembly-enhanced molecular recognition of calixarene. Supramolecular Chemistry: Vol. 30, Calix 2017, pp. 562-567.
Chinese Academy of Medical Sciences & Peking Union Medical College163
Shanghai Jiao Tong University162
Sun Yat-sen University118
Huazhong University of Science and Technology113
Chinese Academy of Sciences110