Vip3Aa is an insecticidal protein that can effectively control certain lepidopteran pests and has been used widely in biological control. However, the mechanism of action of Vip3Aa is unclear. In the present study, we showed that Vip3Aa could cause autophagy in Sf9 cells, which was confirmed by the increased numbers of GFP-Atg8 puncta, the appearance of autophagic vacuoles, and an elevated Atg8-II protein level. Moreover, we found that the AMPK-mTOR-ULK1 pathway is involved in Vip3Aa-induced...

We have designed and prepared four new cellulose cross-linking agents using NaClO/NaBr/TEMPO and sodium periodate selective catalytic oxidation systems. First, the primary hydroxyl group of sucrose, trehalose, maltose, or lactose was carboxylated through NaClO/NaBr/TEMPO system, and then sodium periodate was used to selectively oxidize the carboxyl compound to obtain carboxyl polyaldehyde sugars. They contained multiple aldehyde groups and carboxyl groups in the molecule, had high reactivity, and can be covalently cross-linked with cellulose. To evaluate...

Is SARS-CoV-2 originated from laboratory? A rebuttal to the claim of formation via laboratory recombination

RNA-seq has been widely used to reveal the molecular mechanism of variants of life process. We have developed an alternative method, MustSeq, which generates multiple second strands along a single 1st strand cDNA by random-priming initiation, immediately after reverse transcription for each RNA extract using sample-barcoded poly-dT primers, then 3ʹ ends-enriching PCR is applied to construct the library. Unlike the conventional RNA seq, MustSeq avoids procedures such as mRNA isolation, fragmentation and RNA 5ʹ-end capture,...

Additional file 3: Table S2. Expression of wheat new miRNAs in crown tissues.

Additional file 4: Table S3. Information of wheat_miRNA2693 and wheat_miR5099.

Additional file 8: Table S5. Sequence of primers used in this study.

Macroautophagy/autophagy plays an important role during the development of human cancer. BECN1 (beclin 1), a core player in autophagy regulation, is downregulated in many kinds of malignancy. The underlying mechanism, however, has not been fully illuminated. Here, we found that CUL3 (cullin 3), an E3 ubiquitin ligase, could interact with BECN1 and promote the K48-linked ubiquitination and degradation of this protein; In addition, CUL3 led to a decrease in autophagic activity through downregulating BECN1. We...

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Gut dysbiosis may be implicated in the pathogenesis of IgA nephropathy (IgAN) through immune and/or metabolite pathways. Fecal microbiota transplantation (FMT) could reestablish the micro-ecological balance in IgAN, although this has never been attempted before. We explored whether FMT could be efficacious in treating IgAN in two patients with refractory IgAN. Two Chinese female patients with IgAN failed to achieve clinical remission after receiving several rounds of immunosuppressive therapy and suffered from unbearable adverse effects...

Complement receptor 3 (CD11b/CD18) is an important receptor that mediates adhesion, phagocytosis and chemotaxis in various immunocytes. The conidia of the medically-important pathogenic fungus, Aspergillus fumigatus can be internalized into alveolar epithelial cells to disseminate its infection in immunocompromised host; however, the role of CR3 in this process is poorly understood. In the present study, we investigated the potential role of CR3 on A. fumigatus internalization into type II alveolar epithelial cells and its effect...

Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study aimed to identify potential genes for AAA growth and expansion based on comprehensive bioinformatics approaches. Firstly, 29 up-regulated genes were identified through DEGs analysis between large AAA and small AAA in GSE57691. Secondly,...

Identifying biomarkers for hepatocellular carcinoma (HCC) survival is of great importance for the early detection, monitoring, and predicting for prognosis. This study aimed to investigate the candidate biomarkers for predicting overall survival (OS) in HCC patients. Using RTCGAToolbox, top 50 upregulated differential expressed genes (DEGs) were identified. The least absolute shrinkage and selection operator (LASSO) and Cox models were used to select powerful candidate genes, and log rank method was used to address the survivor...

In this study, a series of new betaine fluorocarbon surfactants (abbreviated as FSC-m) were synthesized with N,N-dimethyl-1,3-propane diamine, perfluoroalkyl sulfonyl fluoride, and sodium chloroacetate. The novel surfactants were characterized by FI-IR, 1H-NMR and 19F-NMR. The surface activity of these surfactants at the air-water interface was investigated. The oil-water interfacial tension, adsorption, and wettability were studied on the oily drilling cuttings. The results showed that the novel surfactants have excellent surface and interfacial activities and low...

In this research, chitosan-decorated activated carbon(AC) was proposed. The AC was cross-linked by glutaraldehyde to prepare adsorbent(AC-CS).The AC-CS has a rough surface. Adding the AC-CS directly to the dye solution can achieve simple and convenient removal of anionic azo dyes acid red 18 (AR-18). In dye solution, the AC-CS was used as adsorbent. The effects of pH, contact time, temperature, initial concentration of AR-18, and the AC-CS dosage on the adsorption efficiency were investigated. Full...

In this research, chitosan-decorated activated carbon (AC-CS) was proposed. The AC was cross-linked with glutaraldehyde to prepare an adsorbent (AC-CS). The AC-CS has a rough surface. Adding the AC-CS directly to the dye solution can achieve simple and convenient removal of anionic azo dyes acid red 18 (AR-18). In the dye solution, the AC-CS was used as an adsorbent. The effects of pH, contact time, temperature, initial concentration of AR-18 and the AC-CS dosage on...

Gallbladder cancer (GBC) is the common malignancy of the bile tract system with extremely poor clinical outcomes, owing to its metastatic property and intrinsic resistance to the first-line drugs. Although it is well-established that cholesterol abnormity contributes to gallstone formation, a leading risk factor for GBC, the link of cholesterol homeostasis with GBC has not been investigated. The present study systematically examined the genes implicated in cholesterol homeostasis, and revealed altered gene expressions of de...

A supramolecular self-assembly has been constructed by perylene-bridged bis(β-cyclodextrin)s with water-soluble porphyrin through hydrophobic interactions, showing strong excitonic coupling interactions between perylene backbones and included porphyrins.

(2015). Molecular recognition of amphiphilic p-sulfonatocalix[4]arene with organic ammoniums. Supramolecular Chemistry: Vol. 27, Ninth International Symposium of Macrocyclic and Supramolecular Chemistry (ISMSC-9), pp. 336-345.

A novel water-soluble perylene bisimide derivative 1 was synthesized with two permethyl-β-cyclodextrin grafts at the imide nitrogens, and its structure was identified by NMR, Fourier transform infrared spectroscopy (FT-IR), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), and elemental analysis. Its aggregation behavior in water and organic solutions was further investigated by UV−vis, fluorescence, and 1H NMR spectra, showing that 1 exhibits strong π···π aggregation in water. Especially, the aggregation and optical properties were comprehensively compared...

The binding behaviors and thermodynamic origins of p-sulfonatocalix[4]arene (C4AS) and p-sulfonatocalix[5]arene (C5AS) with methyl viologen (MV2+) have been investigated by the methods of isothermal titration calorimetry, NMR, and cyclic voltammetry, showing that the binding abilities of C4AS and C5AS and their host selectivity are dramatically pH-controlled, which is closely discussed from the viewpoint of thermodynamics. Moreover, the radical form of MV+• can also be effectively included by C4AS and C5AS.

We herein describe the comprehensive investigation of the complexation behavior of a guanidinium-modified calix[5]arene pentaisohexyl ether (GC5A) with a variety of typical luminescent dyes. Fluorescein, eosin Y, rose bengal, tetraphenylporphine sulfonate and sulfonated aluminum phthalocyanine were employed as classical aggregation-induced quenching dyes. 2-(p-Toluidinyl)naphthalene-6-sulfonic acid and 1-anilinonaphthalene-8-sulfonic acid were selected as representatives of intramolecular charge-transfer dyes. Phosphated tetraphenylethylene was involved as the classical aggregation-induced emission dye. Sulfonated acedan representing one example of two-photon fluorescent probes, was...

(2018). Assembly-enhanced molecular recognition of calix[6]arene. Supramolecular Chemistry: Vol. 30, Calix 2017, pp. 562-567.