Data from: Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencingSchyler O. Nunziata, Jeffrey D. Karron, Randall J. Mitchell, Stacey L. Lance, Kenneth L. Jones & Dorset W. Trapnell
Premise of the study: Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure. Methods and Results: A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single popula- tion in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435. Conclusions: This large number of...
Data from: Direct sequencing of haplotypes from diploid individuals through a modified emulsion PCR-based single-molecule sequencing approachBrian Patrick Hansen Metzger, Gregory William Gelembiuk & Carol Eunmi Lee
While standard DNA sequencing approaches readily yield genotypic sequence data, haplotype information is often of greater utility for population genetic analyses. However, obtaining individual haplotype sequences can be costly and time consuming, and sometimes requires statistical reconstruction approaches that are subject to bias and error. Advancements have recently been made in determining individual chromosomal sequences in large-scale genomic studies, yet few options exist for obtaining this information from large numbers of highly polymorphic individuals in...
Sequential mate choice strategies predict how females should alter their choosiness based on the availability of attractive males. While there are many studies on sequential mate choice within species, few have asked if females apply these strategies to interactions between species and how these strategies may affect hybridization. We tested how previous interactions with conspecific and heterospecific males affect mate preference and sexual isolation in two threespine stickleback species (benthics and limnetics: Gasterosteus spp.). Consistent...
Data from: Wildfire provides refuge from local extinction but is an unlikely driver of outbreaks by mountain pine beetleErinn N. Powell, Philip A. Townsend & Kenneth F. Raffa
Bark beetle outbreaks and wildfire are important disturbances in conifer ecosystems, yet their interactions are not well understood. We evaluated whether fire injury increased susceptibility of lodgepole pines (Pinus contorta) to mountain pine beetle (Dendroctonus ponderosae Hopkins), how it influenced beetle reproductive success, and whether beetle population phase altered this interaction. Eight sites that experienced wildfire and eight unburned sites were examined in the Greater Yellowstone Ecosystem (U.S.A). Half were in areas where D. ponderosae...
Data from: Post-fire changes in forest carbon storage over a 300-year chronosequence of Pinus contorta-dominated forestsDaniel M. Kashian, William H. Romme, Daniel Tinker, Monica G. Turner, Michael G. Ryan & Daniel B. Tinker
A warming climate may increase the frequency and severity of stand-replacing wildfires, reducing carbon (C) storage in forest ecosystems. Understanding the variability of post-fire C cycling on heterogeneous landscapes is critical for predicting changes in C storage with more frequent disturbance. We measured C pools and fluxes for 77 lodgepole pine (Pinus contorta Dougl. ex Loud var. latifolia Engelm.) stands in and around Yellowstone National Park (YNP) along a 300-year chronosequence to examine how quickly...
Data from: The walk is never random: subtle landscape effects shape gene flow in a continuous white-tailed deer population in the Midwestern United StatesStacie J. Robinson, Michael D. Samuel, Davin L. Lopez & Paul Shelton
One of the pervasive challenges in landscape genetics is detecting gene flow patterns within continuous populations of highly mobile wildlife. Understanding population genetic structure within a continuous population can give insights into social structure, movement across the landscape and contact between populations, which influence ecological interactions, reproductive dynamics, or pathogen transmission. We investigated the genetic structure of a large population of deer spanning the area of Wisconsin and Illinois, USA, affected by chronic wasting disease....
Conspicuous innovations in the history of life are often preceded by more cryptic genetic and developmental precursors. In many cases, these appear to be associated with recurring origins of very similar traits in close relatives (parallelisms) or striking convergences separated by deep time (“deep homologies”). Although the phylogenetic distribution of gain and loss of traits hints strongly at the existence of such precursors, no models of trait evolution currently permit inference about their location on...
The positive relationship between species diversity (richness and evenness) and critical ecosystem functions, such as productivity, carbon storage, and nutrient cycling, is often used to predict the consequences of extinction. At regional scales, however, plant species richness is mostly increasing rather than decreasing because successful plant species introductions far outnumber extinctions. If these regional increases in richness lead to local increases in diversity, a reasonable prediction is that productivity, carbon storage, and nutrient cycling will...
University of Wisconsin-Madison8
University of Georgia1
Wayne State University1
University of Wyoming1
United States Department of Agriculture1
National Autonomous University of Mexico1
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Wisconsin Department of Natural Resources1