29 Works

Data from: The microbiota influences the Drosophila melanogaster life history strategy

Amber Walters, Rachel C Hughes, Tanner B. Call, Carson J. Walker, Hailey Wilcox, Samara C. Petersen, Seth M. Rudman, Peter D. Newell, Angela E. Douglas, Paul S. Schmidt & John M. Chaston
Organisms are locally adapted when members of a population have a fitness advantage in one location relative to conspecifics in other geographies. For example, across latitudinal gradients, some organisms may trade off between traits that maximize fitness components in one, but not both, of somatic maintenance or reproductive output. Latitudinal gradients in life history strategies are traditionally attributed to environmental selection on an animal's genotype, without any consideration of the possible impact of associated microorganisms...

Additional file 2 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 2: Supplementary Figs. S1 to S21. Supplementary Fig. S1. Expression in ten melanoma cell lines of genes and gene families targeted by epigenetic drugs. Supplementary Fig. S2. Differentiation profile of melanoma cell lines. Supplementary Fig. S3. Susceptibility of ten melanoma cell lines to the anti-proliferative effects of epigenetic drugs. Supplementary Fig. S4. Methylation status of LINE-1, MAGE-A1 and NY-ESO-1 in 9 melanoma cell lines treated with guadecitabine. Supplementary Fig. S5. Volcano plot of...

Additional file 5 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 5: Supplementary Table S3. Antibodies used in quantitative western blot analysis.

Additional file 7 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 7: Supplementary Table S5. Immune-related gene modulation by epigenetic drugs and by combinatorial treatments.

Additional file 1 of A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Additional file 1: Figure S1. Snapshot of informative pEV-microRNAs of discovery cohort. Figure S2. Principal Component Analysis (PCA) of discovery cohort. Figure S3. Up-regulated pEV-microRNA dotplot of Gene Ontology (GO) enrichment analysis. Figure S4. Down-regulated pEV-microRNA dotplot of Gene Ontology (GO) enrichment analysis. Figure S5. Schematic representation of bioinformatics workflow for pEV-microRNA signature identification in metastatic melanoma cohorts. Figure S6. Heatmap of cell-type enrichment analysis.

A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Abstract Background Melanoma is the deadliest form of skin cancer and metastatic disease is associated with a significant survival rate drop. There is an urgent need for consistent tumor biomarkers to scale precision medicine and reduce cancer mortality. Here, we aimed to identify a melanoma-specific circulating microRNA signature and assess its value as a diagnostic tool. Methods The study consisted of a discovery phase and two validation phases. Circulating plasma extracellular vesicles (pEV) associated microRNA...

Discovery of a new class of triazole based inhibitors of acetyl transferase KAT2A

Roberta Pacifico, Nunzio Del Gaudio, Guglielmo Bove, Lucia Altucci, Lydia Siragusa, Gabriele Cruciani, Menotti Ruvo, Rosa Bellavita, Paolo Grieco & Mauro F. A. Adamo
We have recently developed a new synthetic methodology that provided both N-aryl-5-hydroxytriazoles and N-pyridine-4-alkyl triazoles. A selection of these products was carried through virtual screening towards targets that are contemporary and validated for drug discovery and development. This study determined a number of potential structure target dyads of which N-pyridinium-4-carboxylic-5-alkyl triazole displayed the highest score specificity towards KAT2A. Binding affinity tests of abovementioned triazole and related analogs towards KAT2A confirmed the predictions of the in-silico...

Additional file 9 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 9 Supplementary Table S7. Prognostic significance of genes in the guadecitabine and JQ1 immune-related signatures in the Cancer Genome Atlas (TCGA) melanoma dataset.

Additional file 5 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 5: Supplementary Table S3. Antibodies used in quantitative western blot analysis.

Additional file 2 of A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Additional file 2: Table S1. A detailed list of differentially expressed pEV-microRNAs obtained from high-throughput PCR profiles in metastatic melanoma patients.

Discovery of a new class of triazole based inhibitors of acetyl transferase KAT2A

Roberta Pacifico, Nunzio Del Gaudio, Guglielmo Bove, Lucia Altucci, Lydia Siragusa, Gabriele Cruciani, Menotti Ruvo, Rosa Bellavita, Paolo Grieco & Mauro F. A. Adamo
We have recently developed a new synthetic methodology that provided both N-aryl-5-hydroxytriazoles and N-pyridine-4-alkyl triazoles. A selection of these products was carried through virtual screening towards targets that are contemporary and validated for drug discovery and development. This study determined a number of potential structure target dyads of which N-pyridinium-4-carboxylic-5-alkyl triazole displayed the highest score specificity towards KAT2A. Binding affinity tests of abovementioned triazole and related analogs towards KAT2A confirmed the predictions of the in-silico...

Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Abstract Background Improvement of efficacy of immune checkpoint blockade (ICB) remains a major clinical goal. Association of ICB with immunomodulatory epigenetic drugs is an option. However, epigenetic inhibitors show a heterogeneous landscape of activities. Analysis of transcriptional programs induced in neoplastic cells by distinct classes of epigenetic drugs may foster identification of the most promising agents. Methods Melanoma cell lines, characterized for mutational and differentiation profile, were treated with inhibitors of DNA methyltransferases (guadecitabine), histone...

Additional file 3 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 3 Supplementary Table S1. Origin of melanoma cell lines, histopathological features of the corresponding primary tumor, cell line authentication data by STR profiling, and mutational profile by NGS of 14 melanoma cell lines used in this study.

Additional file 4 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 4: Supplementary Table S2. Drug doses used for experiments and effect of each drug at such dose in the MTT assay on each cell line.

Additional file 6 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 6: Supplementary Table S4. Modulation of immune-related genes belonging to 21 functional classes by epigenetic drugs. Sheets # to #21.

Additional file 2 of A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Additional file 2: Table S1. A detailed list of differentially expressed pEV-microRNAs obtained from high-throughput PCR profiles in metastatic melanoma patients.

A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Abstract Background Melanoma is the deadliest form of skin cancer and metastatic disease is associated with a significant survival rate drop. There is an urgent need for consistent tumor biomarkers to scale precision medicine and reduce cancer mortality. Here, we aimed to identify a melanoma-specific circulating microRNA signature and assess its value as a diagnostic tool. Methods The study consisted of a discovery phase and two validation phases. Circulating plasma extracellular vesicles (pEV) associated microRNA...

Additional file 6 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 6: Supplementary Table S4. Modulation of immune-related genes belonging to 21 functional classes by epigenetic drugs. Sheets # to #21.

Additional file 7 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 7: Supplementary Table S5. Immune-related gene modulation by epigenetic drugs and by combinatorial treatments.

Additional file 8 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 8: Supplementary Table S6. Upstream Regulator analysis on differentially expressed genes in drug-treated vs untreated cell lines VRG100 and CST30.

Additional file 1 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 1. Supplementary Methods.

Additional file 1 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 1. Supplementary Methods.

Additional file 1 of A novel microRNA signature for the detection of melanoma by liquid biopsy

Claudia Sabato, Teresa Maria Rosaria Noviello, Alessia Covre, Sandra Coral, Francesca Pia Caruso, Zein Mersini Besharat, Elena Splendiani, Laura Masuelli, Cecilia Battistelli, Alessandra Vacca, Giuseppina Catanzaro, Agnese Po, Andrea Anichini, Michele Maio, Michele Ceccarelli, Anna Maria Di Giacomo & Elisabetta Ferretti
Additional file 1: Figure S1. Snapshot of informative pEV-microRNAs of discovery cohort. Figure S2. Principal Component Analysis (PCA) of discovery cohort. Figure S3. Up-regulated pEV-microRNA dotplot of Gene Ontology (GO) enrichment analysis. Figure S4. Down-regulated pEV-microRNA dotplot of Gene Ontology (GO) enrichment analysis. Figure S5. Schematic representation of bioinformatics workflow for pEV-microRNA signature identification in metastatic melanoma cohorts. Figure S6. Heatmap of cell-type enrichment analysis.

Additional file 9 of Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Additional file 9 Supplementary Table S7. Prognostic significance of genes in the guadecitabine and JQ1 immune-related signatures in the Cancer Genome Atlas (TCGA) melanoma dataset.

Landscape of immune-related signatures induced by targeting of different epigenetic regulators in melanoma: implications for immunotherapy

Andrea Anichini, Alessandra Molla, Gabriella Nicolini, Valentina Eleonora Perotti, Francesco Sgambelluri, Alessia Covre, Carolina Fazio, Maria Fortunata Lofiego, Anna Maria Di Giacomo, Sandra Coral, Antonella Manca, Maria Cristina Sini, Marina Pisano, Teresa Noviello, Francesca Caruso, Silvia Brich, Giancarlo Pruneri, Andrea Maurichi, Mario Santinami, Michele Ceccarelli, Giuseppe Palmieri, Michele Maio & Roberta Mortarini
Abstract Background Improvement of efficacy of immune checkpoint blockade (ICB) remains a major clinical goal. Association of ICB with immunomodulatory epigenetic drugs is an option. However, epigenetic inhibitors show a heterogeneous landscape of activities. Analysis of transcriptional programs induced in neoplastic cells by distinct classes of epigenetic drugs may foster identification of the most promising agents. Methods Melanoma cell lines, characterized for mutational and differentiation profile, were treated with inhibitors of DNA methyltransferases (guadecitabine), histone...

Registration Year

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  • Biogem
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  • University of Naples Federico II
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