4 Works

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...

Additional file 1 of Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Additional file 1: Figure S1A. Comparison of the absorbance and emission spectra of the different LysoTracker probes. Figure S1B. Comparison of the emission spectra of the different (nano)particles. Figure S1C. Comparison of the absorbance spectra of the different (nano)particles. Figure S2. Confocal microscopy images of different LysoTracker probes. Figure S3. Transmission electron micrographs (TEM) of the different silica (nano)particles. Figure S4A. Size distributions of 59 nm SiO2-BDP FL NP and 59 nm SiO2-RhoB NP measured...

Additional file 1 of Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Additional file 1: Figure S1A. Comparison of the absorbance and emission spectra of the different LysoTracker probes. Figure S1B. Comparison of the emission spectra of the different (nano)particles. Figure S1C. Comparison of the absorbance spectra of the different (nano)particles. Figure S2. Confocal microscopy images of different LysoTracker probes. Figure S3. Transmission electron micrographs (TEM) of the different silica (nano)particles. Figure S4A. Size distributions of 59 nm SiO2-BDP FL NP and 59 nm SiO2-RhoB NP measured...

Registration Year

  • 2022
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Resource Types

  • Collection
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  • Text
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Affiliations

  • Adolphe Merkle Institute
    4
  • University of Fribourg
    4
  • International Iberian Nanotechnology Laboratory
    4