3 Works

Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

Brenda Andrews
Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments — endocytic patch, actin patch,...

The internal region of CtIP functions as a regulator of DNA end resection

Sean Howard, Ilaria Ceppi, Roopesh Anand, Roger Geiger & Petr Cejka
DNA double-strand breaks are repaired by end-joining or homologous recombination. A key-committing step of recombination is DNA end resection. In resection, phosphorylated CtIP first promotes the endonuclease of the MRE11-RAD50-NBS1 (MRN) complex. Subsequently, CtIP also stimulates the BLM-DNA2 helicase-nuclease, coordinating thus both short and long-range resection. The structure of CtIP differs from its orthologues in yeast, as it contains a large internal region. Here we conducted a domain analysis of the internal region of CtIP...

Systematic genetics and single-cell imaging reveal widespread morphological pleiotropy and cell-to-cell variability.

Brenda Andrews
Our ability to understand the genotype-to-phenotype relationship is hindered by the lack of detailed understanding of phenotypes at a single-cell level. To systematically assess cell-to-cell phenotypic variability, we combined automated yeast genetics, high-content screening and neural network-based image analysis of single cells, focussing on genes that influence the architecture of four subcellular compartments of the endocytic pathway as a model system. Our unbiased assessment of the morphology of these compartments — endocytic patch, actin patch,...

Registration Year

  • 2020
    3

Resource Types

  • Image
    2
  • Dataset
    1

Affiliations

  • Institute for Research in Biomedicine
    3
  • RIKEN
    2
  • Memorial Sloan Kettering Cancer Center
    2
  • Institute of Biomedical Research of Barcelona
    2
  • Institució Catalana de Recerca i Estudis Avançats
    2
  • University of Toronto
    2