35 Works

An infectious clone of enterovirus 71(EV71) that is capable of infecting neonatal immune competent mice without adaptive mutations

Huiying Zhang, Zhigang Song, Jingyi Zou, Yanling Feng, Jing Zhang, Lehao Ren, Xiaonan Zhang, Yunwen Hu, Zhenghong Yuan & Zhigang Yi
Enterovirus 71 (EV71) is a major pathogen that causes hand, foot and mouth disease (HFMD), which is a life threatening disease in certain children. The pathogenesis of EV71-caused HFMD is poorly defined due to the lack of simple and robust animal models with severe phenotypes that recapitulate symptoms observed in humans. Here, we generated the infectious clone of a clinical isolate from a severe HFMD patient. Virus rescued from the cDNA clone was infectious in...

Antecedents of purpose in life: Evidence from a lagged exposure-wide analysis

Ying Chen, Eric S. Kim, Alexandra E. Shields & Tyler J. VanderWeele
Potential antecedents to having a sense of purpose in life remain understudied. As researchers begin contemplating purpose as a promising target of public health intervention, it is critical to identify its antecedents. Using prospective data from the Nurses’ Health Study II (2009–2016; N ranged from 3,905 to 4,189), this study evaluated a wide range of potential antecedents of purpose, including psychosocial well-being, psychological distress, employment characteristics, lifestyle, and physical health factors. In separate regression models,...

Meta-analysis of diagnostic performance of serology tests for COVID-19: impact of assay design and post-symptom-onset intervals

Hongyu Wang, Jingwen Ai, Michael J. Loeffelholz, Yi-Wei Tang & Wenhong Zhang
Serology detection is recognized for its sensitivity in convalescent patients with COVID-19, in comparison with nucleic acid amplification tests (NAATs). This article aimed to evaluate the diagnostic accuracy of serologic methods for COVID-19 based on assay design and post-symptom-onset intervals. Two authors independently searched PubMed, Cochrane library, Ovid, EBSCO for case–control, longitudinal and cohort studies that determined the diagnostic accuracy of serology tests in comparison with NAATs in COVID-19 cases and used QUADAS-2 for quality...

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observational cohort study

Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren … & Qinghua Hu
Managing recovered COVID-19 patients with recurrent-positive SARS-CoV-2 RNA test results is challenging. We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. After readmission, recurrent-positive patients exhibited mild (28%) or absent (72%) symptoms, with no disease progression....

Risks and features of secondary infections in severe and critical ill COVID-19 patients

Haocheng Zhang, Yi Zhang, Jing Wu, Yang Li, Xian Zhou, Xin Li, Haili Chen, Mingquan Guo, Shu Chen, Feng Sun, Richeng Mao, Chao Qiu, Zhaoqin Zhu, Jingwen Ai & Wenhong Zhang
Objectives Severe or critical COVID-19 is associated with intensive care unit admission, increased secondary infection rate, and would lead to significant worsened prognosis. Risks and characteristics relating to secondary infections in severe COVID-19 have not been described. Methods Severe and critical COVID-19 patients from Shanghai were included. We collected lower respiratory, urine, catheters, and blood samples according to clinical necessity and culture and mNGS were performed. Clinical and laboratory data were archived. Results We found...

Prolonged presence of viral nucleic acid in clinically recovered COVID-19 patients was not associated with effective infectiousness

Ke Hong, Wei Cao, Zhengyin Liu, Ling Lin, Xing Zhou, Yan Zeng, Yuan Wei, Li Chen, Xiaosheng Liu, Yang Han, Lianguo Ruan & Taisheng Li
Prolonged presence of viral nucleic acid was reported in certain patients with coronavirus disease 2019 (COVID-19), with unclear clinical and epidemiological significance. We here described the clinical and epidemiological characteristics of 37 recovered COVID-19 patients with prolonged presence of viral RNA in Wuhan, China. For those who had been discharged and re-admitted, their close contacts outside the hospital were traced and evaluated. The median age of the 37 patients was 62 years (IQR 50, 68),...

Long noncoding RNA MEG3 suppresses cell proliferation, migration and invasion, induces apoptosis and paclitaxel-resistance via miR-4513/PBLD axis in breast cancer cells

Mingzhi Zhu, Fang Wang, Hailong Mi, Lin Li, Jing Wang, Mingli Han & Yuanting Gu
Breast cancer remains a general-threat event in the health of women. Currently, increasing records indicate that long non-coding RNA maternally expressed 3 (MEG3) plays a central role in breast cancer. The current research focused on the function of MEG3 in paclitaxel (PTX)-resistance and human breast cancer growth. Levels of MEG3, microRNA (miR)-4513, and phenazine biosynthesis-like domain-containing protein (PBLD) were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) or western blot assays. 3-(4.5-dimethylghiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay...

Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis

Yan Wang, Quande Lin, Chunge Song, Ruojin Ma & Xiaojie Li
Circular RNAs (circRNAs) possess important regulatory effects on multiple myeloma (MM) progression. Here, we aimed at exploring the function of circ_0007841 in MM and the underlying molecular mechanism. Expression of circ_0007841, microRNA (miR)-129-5p and Jagged1 (JAG1) was determined via qRT-PCR or western blot assay. Methyl thiazolyl tetrazolium (MTT) assay was applied to examine cell viability and IC50 value of MM cells to bortezomib (BTZ). Colony formation assay was performed to analyze cell proliferation. Moreover, cell...

Presence and persistence of hepatitis E virus RNA and proteins in human bone marrow

Lin Wang, Li Yan, Jieling Jiang, Yuyi Zhang, Qiyu He, Hui Zhuang & Ling Wang
Hepatitis E virus (HEV) infection is primarily manifesting as acute hepatitis, but extra-hepatic replication and injury are frequently reported. During the study period, we discovered two acute myeloid leukaemia (AML) patients infected with HEV genotype 3 and 4, respectively, and HEV RNA and/or viral proteins were persistently detected in the bone marrow of both patients. The finding suggests that HEV can replicate in human bone marrow as it may serve as a new target site...

L-lysine potentiates aminoglycosides against Acinetobacter baumannii via regulation of proton motive force and antibiotics uptake

Wanyan Deng, Tiwei Fu, Zhen Zhang, Xiao Jiang, Jianping Xie, Hang Sun, Peng Hu, Hong Ren, Peifu Zhou, Qi Liu & Quanxin Long
Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is a leading cause of hospital- and community-acquired infections. Acinetobacter baumannii can rapidly acquire diverse resistance mechanisms and undergo genetic modifications that confer resistance and persistence to all currently used clinical antibiotics. In this study, we found exogenous L-lysine sensitizes Acinetobacter baumannii, other Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and a Gram-positive bacterium (Mycobacterium smegmatis) to aminoglycosides. Importantly, the combination of L-lysine with aminoglycosides killed clinically isolated multidrug-resistant...

Risks and features of secondary infections in severe and critical ill COVID-19 patients

Haocheng Zhang, Yi Zhang, Jing Wu, Yang Li, Xian Zhou, Xin Li, Haili Chen, Mingquan Guo, Shu Chen, Feng Sun, Richeng Mao, Chao Qiu, Zhaoqin Zhu, Jingwen Ai & Wenhong Zhang
Objectives Severe or critical COVID-19 is associated with intensive care unit admission, increased secondary infection rate, and would lead to significant worsened prognosis. Risks and characteristics relating to secondary infections in severe COVID-19 have not been described. Methods Severe and critical COVID-19 patients from Shanghai were included. We collected lower respiratory, urine, catheters, and blood samples according to clinical necessity and culture and mNGS were performed. Clinical and laboratory data were archived. Results We found...

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains

Xiaoli Wu, Yanrong Zheng, Mengru Liu, Yue Li, Shijia Ma, Weidong Tang, Wenping Yan, Ming Cao, Wanqing Zheng, Lei Jiang, Jiaying Wu, Feng Han, Zhenghong Qin, Liang Fang, Weiwei Hu, Zhong Chen & Xiangnan Zhang
Mitophagy, the elimination of damaged mitochondria through autophagy, promotes neuronal survival in cerebral ischemia. Previous studies found deficient mitophagy in ischemic neurons, but the mechanisms are still largely unknown. We determined that BNIP3L/NIX, a mitophagy receptor, was degraded by proteasomes, which led to mitophagy deficiency in both ischemic neurons and brains. BNIP3L exists as a monomer and homodimer in mammalian cells, but the effects of homodimer and monomer on mitophagy are unclear. Site-specific mutations in...

Corticosteroid prevents COVID-19 progression within its therapeutic window: a multicentre, proof-of-concept, observational study

Yang Li, Xian Zhou, Tao Li, Shiji Chan, Yiqi Yu, Jing-Wen Ai, Haocheng Zhang, Feng Sun, Qiran Zhang, Lei Zhu, Lingyun Shao, Bin Xu & Wenhong Zhang
Critically ill patients with coronavirus diseases 2019 (COVID-19) are of grave concern. Those patients usually underwent a stage of excessive inflammation before developing acute respiratory distress syndrome. In this study, we test the hypothesis that short-term, low-to-moderate-dose corticosteroids would benefit patients when used in the early phase of excessive inflammation, namely, the therapeutic window. Among a Shanghai cohort and a validation cohort, we enrolled COVID-19 patients showing marked radiographic progression. Short-term, low-to-moderate-dose corticosteroids were considered...

A simple electrochemical method for Cd(II) determination in real samples based on carbon nanotubes and metal-organic frameworks

Ling Yu, Jing-Wei Wan, Xian-Zhu Meng, Hui-Wen Gu, Ying Chen & Hong-Chao Yi
The establishment of a simple and effective method for determination of heavy metal ions has great significance due to the high hazard of heavy metal ions to public health and environments. Herein, we developed a facile way to synthesise a nanocomposite comprised of carbon nanotubes (CNTs) and metal-organic frameworks (MOFs), and explored its application in constructing an electrochemical (EC) sensor for detection of Cd2+ in aqueous solutions. The sensing surface was simply fabricated by casting...

Corticosteroid prevents COVID-19 progression within its therapeutic window: a multicenter, proof-of-concept, observational study.

Yang Li, Xian Zhou, Tao Li, Shiji Chan, Yiqi Yu, Jing-Wen Ai, Haocheng Zhang, Feng Sun, Qiran Zhang, Lei Zhu, Lingyun Shao, Bin Xu & Wenhong Zhang
Background: Critically ill patients with COVID-19 are of grave concern. Those patients usually underwent a stage of excessive inflammation before developing acute respiratory distress syndrome. In this study, we test the hypothesis that short-term, low-to-moderate-dose corticosteroids would benefit patients when used in the early phase of excessive inflammation namely, the therapeutic window. Methods: Among a Shanghai cohort and a validation cohort, we enrolled COVID-19 patients showing marked radiographic progression. Short-term, low-to-moderate-dose corticosteroids were considered for...

Immune responses of a CV-A16 live attenuated candidate strain and its protective effects in rhesus monkeys

Ting Yang, Tianhong Xie, Hua Li, Xia Song, Lei Yue, Xi Wang, Dong Shen, Kaili Ma, Qinfang Jiang, Runxiang Long, Rong Yang, Xin He, Ye Zhang, Zhongping Xie & Qihan Li
Coxsackievirus A16 (CV-A16) is a major causative pathogen of hand, foot, and mouth diseases (HFMDs). The licensed HFMD vaccine targets EV-A71 without cross-protection against CV-A16. Thus, a CV-A16 vaccine is needed. In this study, the immunogenicity and protective efficacy of a live attenuated CV-A16 candidate, K168-8Ac, were evaluated in a rhesus monkey model. Four passages of this strain (P35, P50, P60, and P70) were administered to monkeys, and its protective effect was identified. The immunized...

Sugar transfer of nanomaterials and flexible electrodes

Tianzeng Hong, Tianze Shen, Junlong Yang, Yichen Sun, Jianming Zhang, Haiyang Pan, Ying Hong, Yan Wang, Shuo Chen, Yue Zhao & Chuan Fei Guo
Nanomaterials with various dimensionalities (e.g., nanowires, nanofilms, two-dimensional materials, and three-dimensional nanostructures) have shown great potential in the recent development of flexible electronics. Conventionally, organic solvents are inevitable while integrating nanomaterials onto flexible substrates, where polymer mediator-assisted transfer techniques are involved. This often damages the flexible substrate and thus hamper the large-scale application of nanomaterials. Here we report a method using water-soluble sugar as a mediator to facilely transfer nanomaterials onto rigid or flexible substrates....

Hepatic stellate cell reprogramming via exosome-mediated CRISPR/dCas9-VP64 delivery

Nianan Luo, Jiangbin Li, Yafeng Chen, Yan Xu, Yu Wei, Jianguo Lu & Rui Dong
Hepatic stellate cells (HSCs) play a crucial role in the progression of liver fibrosis, which can be considered as the specific therapeutic target of anti-fibrotic treatment. Targeted induction of HSCs to hepatocytes via delivery of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (dCas9) system holds promise for hepatic fibrosis treatment. Our study here revealed that CRISPR/dCas9-VP64 system encapsulated in AML12 cell-derived exosomes could efficiently and successfully be delivered into the HSCs. In turn,...

Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis

Yan Wang, Quande Lin, Chunge Song, Ruojin Ma & Xiaojie Li
Circular RNAs (circRNAs) possess important regulatory effects on multiple myeloma (MM) progression. Here, we aimed at exploring the function of circ_0007841 in MM and the underlying molecular mechanism. Expression of circ_0007841, microRNA (miR)-129-5p and Jagged1 (JAG1) was determined via qRT-PCR or western blot assay. Methyl thiazolyl tetrazolium (MTT) assay was applied to examine cell viability and IC50 value of MM cells to bortezomib (BTZ). Colony formation assay was performed to analyze cell proliferation. Moreover, cell...

Prolonged presence of viral nucleic acid in clinically recovered COVID-19 patients was not associated with effective infectiousness

Ke Hong, Wei Cao, Zhengyin Liu, Ling Lin, Xing Zhou, Yan Zeng, Yuan Wei, Li Chen, Xiaosheng Liu, Yang Han, Lianguo Ruan & Taisheng Li
Prolonged presence of viral nucleic acid was reported in certain patients with coronavirus disease 2019 (COVID-19), with unclear clinical and epidemiological significance. We here described the clinical and epidemiological characteristics of 37 recovered COVID-19 patients with prolonged presence of viral RNA in Wuhan, China. For those who had been discharged and re-admitted, their close contacts outside the hospital were traced and evaluated. The median age of the 37 patients was 62 years (IQR 50, 68),...

Chiral 4-O-acylterpineol as transdermal permeation enhancers: insights of the enhancement mechanisms of a transdermal enantioselective delivery system for flurbiprofen

Tianzhe Chu, Chunyan Wang, Jing Wang, Heping Wang, Dandan Geng, Chensi Wu, Linlin Zhao & Ligang Zhao
In order to devise more effective penetration enhancers, 4-O-acylterpineol derivatives which were expected to be hydrolyzed into nontoxic metabolites by esterase in the living epidermis, were synthesized from 4-terpineol (4-TER) enantiomers and straight chain fatty acids. Their promoting activities on the SR-flurbiprofen and its enantiomers were tested across full-thickness rabbit skin, as well as to correlate under in vitro and in vivo conditions. The permeation studies indicated that both d-4-O-acylterpineol and l-4-O-acylterpineol had significant enhancing...

Long noncoding RNA MEG3 suppresses cell proliferation, migration and invasion, induces apoptosis and paclitaxel-resistance via miR-4513/PBLD axis in breast cancer cells

Mingzhi Zhu, Fang Wang, Hailong Mi, Lin Li, Jing Wang, Mingli Han & Yuanting Gu
Breast cancer remains a general-threat event in the health of women. Currently, increasing records indicate that long non-coding RNA maternally expressed 3 (MEG3) plays a central role in breast cancer. The current research focused on the function of MEG3 in paclitaxel (PTX)-resistance and human breast cancer growth. Levels of MEG3, microRNA (miR)-4513, and phenazine biosynthesis-like domain-containing protein (PBLD) were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) or western blot assays. 3-(4.5-dimethylghiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay...

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains

Xiaoli Wu, Yanrong Zheng, Mengru Liu, Yue Li, Shijia Ma, Weidong Tang, Wenping Yan, Ming Cao, Wanqing Zheng, Lei Jiang, Jiaying Wu, Feng Han, Zhenghong Qin, Liang Fang, Weiwei Hu, Zhong Chen & Xiangnan Zhang
Mitophagy, the elimination of damaged mitochondria through autophagy, promotes neuronal survival in cerebral ischemia. Previous studies found deficient mitophagy in ischemic neurons, but the mechanisms are still largely unknown. We determined that BNIP3L/NIX, a mitophagy receptor, was degraded by proteasomes, which led to mitophagy deficiency in both ischemic neurons and brains. BNIP3L exists as a monomer and homodimer in mammalian cells, but the effects of homodimer and monomer on mitophagy are unclear. Site-specific mutations in...

Colistin-resistance gene mcr in clinical carbapenem-resistant Enterobacteriaceae strains in China, 2014–2019

Hong Huang, Ning Dong, Lingbin Shu, Jiayue Lu, Qiaoling Sun, Edward Wai-Chi Chan, Sheng Chen & Rong Zhang
To investigate whether introduction of colistin into the clinical settings selected colistin-resistant CRE, we performed molecular epidemiological study of 1868 CRE strains collected from different geographical locales in China during the period 2014–2019. 1755 (96.18%) isolates carried the carbapenemase genes blaKPC and blaNDM; 14 Escherichia coli isolates (0.75%) carrying mcr-1 and blaNDM (MCR-CREC) were also identified. Importantly, the number and relative prevalence of MCR-CREC isolates increased from 5 (0.41%) to 9 (1.38%) after introduction of...

Registration Year

  • 2020
    35

Resource Types

  • Text
    18
  • Dataset
    14
  • Image
    2
  • Other
    1

Affiliations

  • Shanghai Medical College of Fudan University
    35
  • Sichuan University
    29
  • Shanghai Jiao Tong University
    27
  • Sun Yat-sen University
    23
  • Zhejiang University
    23
  • Fudan University Shanghai Cancer Center
    23
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    23
  • Fudan University
    20
  • First Affiliated Hospital of Chongqing Medical University
    19
  • Huazhong University of Science and Technology
    18