Oxidative stress and chronic inflammation are major culprits of nonalcoholic fatty liver disease (NAFLD). MicroRNA-665-3p (miR-665-3p) is implicated in regulating inflammation and oxidative stress; however, its role and molecular basis in NAFLD remain elusive. Herein, we measured a significant upregulation of miR-665-3p level in the liver and primary hepatocytes upon high fat diet (HFD) or 0.5 mmol/L palmitic acid plus 1.0 mmol/L oleic acid stimulation, and the elevated miR-665-3p expression aggravated oxidative stress, inflammation and...

This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Dysregulation of macroautophagy/autophagy has been closely implicated in aging. Caloric restriction (CR) is an effective intervention of aging partially via activation of autophagy. Recently, a high-throughput single-cell RNA-seq technique has been employed to detect the comprehensive transcriptomes of individual cells. However, the transcriptional networks of ATG (autophagy related) genes in the aging process and the modulation of ATG genes expression by CR at the single-cell level have not been elucidated. Here, by performing data analysis...

Background. Far-field electrograms from superior vena cava (SVC) can be present in right superior pulmonary vein (RSPV) after pulmonary vein (PV) isolation. Objectives. To analyze the characteristics of far-field SVC potentials in RSPV after PV isolation and the local anatomy difference between patients with and without the potentials. Methods. Patients undergoing PV isolation were retrospectively reviewed, contrast-enhanced computed tomography (CT) was performed before procedure for observing the anatomical relationship between RSPV and SVC. The prevalence...

This dataset contains all of the data underlying the figures in the accompanying paper "70% Increase in Northwest China’s NOx Emissions Driven by Increases from New Sources and Simultaneous Reductions in Well-Regulated Sources". These are listed respectively as: fig2.mat fig3.mat fig4.mat fig5.mat fig6.mat All subplots are respectively given as fig2a, fig2b, etc.
The daily emissions dataset, and monthly a1, a2, and a3 datasets are respectively found in the file dailyresults.mat
Supplemental Figures S1-ii,...

A pot experiment was performed to investigate the effect of phytoremediation (CK, using tall fescue), fungi remediation (GV, using Glomus versiforme), bacterial remediation (PS, using Pseudomonas fluorescens Ps2-6), and microbial-phytoremediation (GVPS, using three species) on removing polycyclic aromatic hydrocarbons (PAHs) and the microbial diversity in soils. Inoculation with G. versiforme and P. fluorescens could increase the biomass of tall fescue and the accumulation of phenanthrene (PHE) and pyrene (PYR) in plants. Among them, the highest...

Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

Bladder cancer can range from noninvasive to invasive tumors. When non-muscle invasive bladder cancer (NMIBC) recurs, patients could endure long-term invasive malignancies with a high disease-specific death rate. Immune escape frequently results in tumor development, metastases, unfavorable prognosis, and failure of immunotherapy. Based on the median immune score, this study used ESTIMATE scores to evaluate 411 bladder cancer cases from TCGA-BLCA. Two hundred two ncRNAs were differentially expressed in two groups, where 29 candidates appeared...

Drought plays a prominent role in affecting ecosystem stability and ecosystem productivity. Based on eddy covariance and climatic observations during 2012–2020, the Fisher discriminant analysis method was employed to accurately detect drought occurrences. Furthermore, the ecosystem water sensitivity and its resistance to drought were quantified to evaluate the ecosystem stability. The results showed that the alpine meadow suffered drought most frequently at the beginning of the growing seasons. However, drought during the peak growing seasons...

Geospatial studies must address spatial data quality, especially in data-driven research. An essential concern is how to fill spatial data gaps (missing data), such as for cartographic polylines. Recent advances in deep learning have shown promise in filling holes in images with semantically plausible and context-aware details. In this paper, we propose an effective framework for vector-structured polyline completion using a generative model. The model is trained to generate the contents of missing polylines of...

The domestic yak was regarded as a valuable model to explore the molecular mechanism of high-altitude animal adaptation, including the extreme tolerance of starvation and cold stress, which is closely associated with specifically seasonal deposition and metabolism of subcutaneous adipose tissue. However, the key regulators for these processes remain largely unknown. Here, the yak adipogenesis model in vitro was established and liver kinase b1 (LKB1) was regarded as a key negative regulator involved in yak...

The molecular events underlying the lactation curve of dairy animals have long been a biological conundrum for mammary biologists, and a cause of a considerable loss of income for dairy farmers. To better understand the regulatory mechanisms and developmental processes of the mammary gland during the lactation cycle, whole-genome transcriptome profiles of bovine mammary tissues at five different lactation stages were investigated using the RNA-Seq technique. The differentially expressed genes were identified by comparing different...

1. The following study examined the expression profiles of the receptor tyrosine kinases (RTK) family in the developing feather follicles of Pekin ducks and Nonghua ducks at 6-9 weeks of age. The RTK subfamilies related to feather development were summarised, and other candidate genes related to feather development were enriched. 2. To reveal the potential role of all RTK members in feather development, the feather follicles of two duck breeds (Pekin and Nonghua ducks) at...

Long noncoding RNA LINRIS (LINC00920) is known to participate in colorectal cancer. This study aimed to explore the role of LINRIS in non-small cell lung cancer (NSCLC). NSCLC and adjacent non-tumor tissues were collected from 62 NSCLC patients. LINRIS expression was detected using real-time quantitative PCR (RT-qPCRs). The 62 NSCLC patients were monitored every month for 5 years to evaluate the role of LINRIS in predicting the prognosis of NSCLC. The effects of LINRIS silencing...

Patients with post-traumatic stress disorder (PTSD) are usually at an increased risk for chronic disorders, such as irritable bowel syndrome (IBS), characterized by hyperalgesia and allodynia, but its subsequent effect on visceral hyperalgesia and the mechanism remain unclear. The present study employed single prolonged stress (SPS), a model of PTSD-pain comorbidity, behavioral evaluation, intrathecal drug delivery, immunohistochemistry, Western blotting, and RT-PCR techniques. When detecting visceral sensitivity, the score of the abdominal withdrawal reflex (AWR) induced...

Sponge iron (SI) is widely used in water treatment. As effluents from wastewater treatment plant (WWTP) require advanced treatment methodology, three forms of constructed wetlands (CWs): wetlands with sponge iron (SI), copper sulfate modified sponge iron (Cu/SI), and sponge iron coupled with solid carbon sources (C/SI), have been investigated in this paper for the removal effects of organic matter and nutrients in WWTP effluents, and the corresponding mechanisms have been analyzed. The results showed the...

This dataset contains all of the data underlying the figures in the accompanying paper "New Model-Free Daily Inversion of NOx Emissions using TROPOMI (MCMFE-NOx) and its Uncertainty: Deducing a See-Saw of Halved Well Regulated Sources and Doubled New Sources". These are listed respectively under: Coefficients.zip Figure3.zip Figure4.zip Figure5.zip Figure6.zip Figure7.zip Figure8.zip Figure9.zip Figure10.zip All data is provided as .nc files, and all plots are provided as .png files. All emissions are provided as a part...

This study aimed to identify physiological and pharmacogenomic covariates and develop a population pharmacokinetic model of high-dose methotrexate (HD-MTX) in Chinese paediatric patients with acute lymphoblastic leukaemia (ALL) and malignant lymphoma.A total of 731 MTX courses and 1658 MTX plasm concentrations from 205 paediatric patients with ALL and malignant lymphoma were analysing using a non-linear mixed-effects model technique. 47 SNPs in 16 MTX-related genes were genotyped and screened as covariates. A PPK model was established...

Cotton (Gossypium hirsutum) is an important economic crop, so it is of great significance to analyze its molecular mechanisms of resistance to Verticillium Wilt. The WRKY gene family encodes transcription factors (TFs) involved in plant resistance to biotic and abiotic stress. Here, we identified 17 group III WRKY genes in cotton based on the cotton genome project. The phylogenetic relationship and evolutionary of WRKY Group III TFs in Arabidopsis thaliana and G. hirsutum showed that...

Blueberries are rich in anthocyanins, which are plant-specific secondary metabolites that are a good source of plant nutrients. Although anthocyanins have been investigated, there is a lack of comprehensive detailed research that includes large numbers of blueberry cultivars, and the anthocyanin compositions of some cultivars are still unknown. Consequently, we determined the anthocyanin compositions of 62 cultivars using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). In total, we identified 30 anthocyanins derived from five kinds of anthocyanidin....

Sulfate transporters (SULTRs) are responsible for the absorption and transport of sulfate in higher plants. They also play a key role in plant growth, development and abiotic stress responses. However, the SULTR gene family in common bean (Phaseolus vulgaris L.) is still not well studied. In this study, 15 PvSULTRs were identified. Phylogenetic analysis divided the PvSULTR genes into four subgroups. Cis-regulatory element analysis of the promoter sequence of the PvSULTR genes showed that many...

Sulfate transporters (SULTRs) are responsible for the absorption and transport of sulfate in higher plants. They also play a key role in plant growth, development and abiotic stress responses. However, the SULTR gene family in common bean (Phaseolus vulgaris L.) is still not well studied. In this study, 15 PvSULTRs were identified. Phylogenetic analysis divided the PvSULTR genes into four subgroups. Cis-regulatory element analysis of the promoter sequence of the PvSULTR genes showed that many...

Directly drawing metamaterials on paper based on an automatic drawing machine: supplemental document

Additional file 1. Original images for western blot.