6,926 Works

Effect of Antidiabetic Therapy on Clinical Outcomes of COVID-19 Patients With Type 2 Diabetes: A Systematic Review and Meta-Analysis

Kegang Zhan, Liuqi Weng, Li Qi, Luhan Wang, Hao Lin, Xiaoyu Fang, Hong Jia & Xiangyu Ma
Background:No study has yet systematically evaluated the effect of antidiabetic therapy on clinical outcomes of COVID-19 patients with type 2 diabetes (T2D).Objective:We aimed to evaluate the effect of different antidiabetic therapy on clinical outcomes of COVID-19 patients with T2D.Methods:We comprehensively retrieved the published research which examined the effect of antidiabetic therapy on clinical outcomes of COVID-19 patients with T2D. The odds ratio (OR) and its 95% confidence interval (95% CI) for clinical outcomes were calculated...

m6A-immune-related lncRNA prognostic signature for predicting immune landscape and prognosis of bladder cancer

Zi-Hao Feng, Yan-Ping Liang, Jun-Jie Cen, Hao-Hua Yao, Hai-Shan Lin, Jia-Ying Li, Hui Liang, Zhu Wang, Qiong Deng, Jia-Zheng Cao, Yong Huang, Jin-Huan Wei, Jun-Hang Luo, Wei Chen & Zhen-Hua Chen
Abstract Background N6-methyladenosine (m6A) related long noncoding RNAs (lncRNAs) may have prognostic value in bladder cancer for their key role in tumorigenesis and innate immunity. Methods Bladder cancer transcriptome data and the corresponding clinical data were acquired from the Cancer Genome Atlas (TCGA) database. The m6A-immune-related lncRNAs were identified using univariate Cox regression analysis and Pearson correlation analysis. A risk model was established using least absolute shrinkage and selection operator (LASSO) Cox regression analyses, and...

Facile approach for surfactant-free synthesis of Au@ginsenoside Rh2 nanoparticles and researches on anticancer activity

Hua Yao, Xupeng Mu, Zhenhong Wei, Xiuying Li, Liya Wu, Yongri Jin, Xuwen Li, Jing Li & Jinlan Jiang
Abstract Background Inorganic nanocomposites especially Au nanostructures have exhibited outstanding physicochemical properties in biomedical fields. For further clinical applications on theranostics, especially drug delivery, numerous explorations of green and facile synthesis methods combining with pharmacoactive natural components have been investigated to construct safe and multifunctional bioactive Au nanoparticles (NPs). Ginsenoside Rh2 is protopanaxadiol type compound isolated from plants of genus Panax, with excellent anticancer effect and antioxidant activity. In this research, we prepared the novel...

Additional file 1 of LncRNA XIST from the bone marrow mesenchymal stem cell derived exosome promotes osteosarcoma growth and metastasis through miR-655/ACLY signal

Guanghui Zhu, Yu Xia, Ziyue Zhao, Aoyu Li, Hui Li & Tao Xiao
Additional file 1: Figure S1. Characterization of BMSCs. A Morphology of BMSCs under light microscope; B, C Flow cytometry and statistical analysis of the positive rates of MSC-specific surface markers (CD29 and CD90) and a hematopoietic marker CD45 (n = 3); D The analysis of ALP level after osteogenesis induction 14d(n = 3); E The level of mineralized nodules analyzed with Alizarin red staining after osteogenesis induction 21d (n = 3); F The level of...

Additional file 4 of SIRPα antibody combined with oncolytic virus OH2 protects against tumours by activating innate immunity and reprogramming the tumour immune microenvironment

Defeng Kong, Zhenrong Yang, Guoliang Li, Quanyou Wu, Zhaoru Gu, Duo Wan, Qi Zhang, Xiaoli Zhang, Shujun Cheng, Binlei Liu, Kaitai Zhang & Wen Zhang
Additional file 4. Supplementary methods.

Additional file 1 of SIRPα antibody combined with oncolytic virus OH2 protects against tumours by activating innate immunity and reprogramming the tumour immune microenvironment

Defeng Kong, Zhenrong Yang, Guoliang Li, Quanyou Wu, Zhaoru Gu, Duo Wan, Qi Zhang, Xiaoli Zhang, Shujun Cheng, Binlei Liu, Kaitai Zhang & Wen Zhang
Additional file 1: Table S1. Gene lis for heatmap.

Ambient air pollution associated with incidence and dynamic progression of type 2 diabetes: a trajectory analysis of a population-based cohort

Yinglin Wu, Shiyu Zhang, Samantha E. Qian, Miao Cai, Haitao Li, Chongjian Wang, Hongtao Zou, Lan Chen, Michael G. Vaughn, Stephen Edward McMillin & Hualiang Lin
Abstract Background Though the association between air pollution and incident type 2 diabetes (T2D) has been well documented, evidence on the association with development of subsequent diabetes complications and post-diabetes mortality is scarce. We investigate whether air pollution is associated with different progressions and outcomes of T2D. Methods Based on the UK Biobank, 398,993 participants free of diabetes and diabetes-related events at recruitment were included in this analysis. Exposures to particulate matter with a diameter...

Additional file 1 of Ambient air pollution associated with incidence and dynamic progression of type 2 diabetes: a trajectory analysis of a population-based cohort

Yinglin Wu, Shiyu Zhang, Samantha E. Qian, Miao Cai, Haitao Li, Chongjian Wang, Hongtao Zou, Lan Chen, Michael G. Vaughn, Stephen Edward McMillin & Hualiang Lin
Additional file 1: Details of outcomes. Table S1. The UDI, definition and measurements of covariates. Table S2. Characteristics of the participants included or excluded in the study. Table S3. Distributions of the annual average exposures among 398,993 participants. Table S4. Associations between air pollution and risk of cause-specific mortality. Table S5. Results of 2-pollutant models. Table S6. Results of sensitivity analyses in the model including traffic noise (n = 393,515). Table S7. Results of sensitivity...

Additional file 8 of Satisfactory breeding potential is transiently eliminated in beef bulls with clinical anaplasmosis

Anne C. Lovett, Emily J. Reppert, John R. Jaeger, Qing Kang, Macy R. Flowers, Naemi P. Bickmeier, Tippawan Anantatat, Shannon C. O’Day, Chance L. Armstrong & Kathryn E. Reif
Supplementary Material 8

Satisfactory breeding potential is transiently eliminated in beef bulls with clinical anaplasmosis

Anne C. Lovett, Emily J. Reppert, John R. Jaeger, Qing Kang, Macy R. Flowers, Naemi P. Bickmeier, Tippawan Anantatat, Shannon C. O’Day, Chance L. Armstrong & Kathryn E. Reif
Abstract Background Natural service breeding is common in U.S. cow-calf operations. Diseases impacting bull reproductive performance have significant economic consequences for producers. Anaplasmosis may be an underappreciated cause of poor reproductive performance in bulls. The primary systemic effects of bovine anaplasmosis including anemia, fever, and weight loss, can all result in unsatisfactory reproductive performance. The objective of this pilot study was to evaluate breeding soundness examination (BSE) outcomes and clinical changes in bulls during and...

Additional file 2 of Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip

Xiaofang Xu, Yiguo Jia, Ruolin Li, Yuting Wen, Yuchen Liang, Guangjie Lao, Xiaojuan Liu, Wei Zhou, Huawei Liu, Jiang Xie, Xiaoxia Wang, Wenming Xu & Qun Sun
Additional file 2. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for C. albicans.

Additional file 1 of Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Additional file 1: Table S1. Significantly changed metabolites at 15 min. Table S2. Significantly changed metabolites at 1 h. Table S3. Significantly changed metabolites at 4 h. Table S4. Significantly changed metabolites at 24 h. Table S5. DEGs induced by polymyxin B at 1 h. Table S6. DEGs induced by rifampicin B at 1 h. Table S7. DEGs induced by the combination of polymyxin B and rifampicin at 1 h. Table S8. DEGs induced by...

Additional file 2 of Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Additional file 2: Figure S1. Time-kill kinetics of polymyxin B (PolyB; 1 mg/L), rifampicin (Rif; 2 mg/L), and their combination against P. aeruginosa PAO1 at starting 600 nm (OD600) of ~ 0.5 (~ 108 CFU/mL). Figure S2. GO enrichment analysis of the significantly changed genes with the polymyxin B/rifampicin combination at (A) 1 h and (B) 24 h: (i) downregulation and (ii) upregulation of various biological processes. Figure S3. Overview of metabolic pathways of P....

Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Abstract Background Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin–rifampicin combination. Methods Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination...

Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Abstract Background Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin–rifampicin combination. Methods Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination...

Silencing eL31 suppresses the progression of colorectal cancer via targeting DEPDC1

Gaowa Sharen, Xiongfeng Li, Jiaxin Sun, Lei Zhang, Wen Xi, Xiaodong Zhao, Fei Han, Longlong Jia, Rong A, Haidong Cheng & Mingxing Hou
Abstract Background Colorectal cancer (CRC) is one of the most commonly diagnosed human malignancies. Ribosomal protein L31 (RPL31, aka eL31) is a component of the 60S large ribosomal subunit, and its expression pattern and functional role in CRC have not been reported. Methods Herein, we identified that eL31 protein level was dramatically increased in CRC tissues through using IHC analysis. More notably, elevated eL31 was associated with larger tumor size and shorter overall survival. Besides,...

Additional file 1 of Silencing eL31 suppresses the progression of colorectal cancer via targeting DEPDC1

Gaowa Sharen, Xiongfeng Li, Jiaxin Sun, Lei Zhang, Wen Xi, Xiaodong Zhao, Fei Han, Longlong Jia, Rong A, Haidong Cheng & Mingxing Hou
Additional file 1: Figure S1. (A) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 cells were detected by qRT-PCR. (B) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 and RKO cells were evaluated through observing the fluorescence inside cells. Magnification times: 200×. (C) The densitometric analyses of Akt, P-Akt, CCND1, CDK6 and PIK3CA proteins expression. * P < 0.05, *** P < 0.001. Figure S2. (A) The volcano plot of gene...

Identification of the origin of tumor in vein: comparison between CEUS LI-RADS v2017 and v2016 for patients at high risk

Wen-juan Tong, Mei-qing Cheng, Man-xia Lin, Hang-tong Hu, Jia-min Pan, Hui Huang, Ying Wang, Xiao-yan Xie, Ming-de Lu, Ming Kuang, Yang Huang & Wei Wang
Abstract Objectives To compare the diagnostic performance of the Contrast-Enhanced Ultrasound (CEUS) Liver Imaging Report and Data System (LI-RADS) v2016 and v2017 in identifying the origin of tumor in vein (TIV). Methods From April 2014 to December 2018, focal liver lesions (FLLs) accompanied by TIV formation in patients at high risk for hepatocellular carcinoma (HCC) were enrolled. Histologic evaluation or composite imaging reference standard were served as the reference standard. Each case was categorized according...

Additional file 1 of H2O2/pH Dual-Responsive Biomimetic Nanoenzyme Drugs Delivery System for Enhanced Tumor Photodynamic Therapy

Xinyuan Li, Qing Ji, Chao Yan, Ziyu Zhu, Zhihui Yan, Ping Chen, Yisen Wang & Li Song
Additional file 1. Fig. S1. Narrow XPS scan spectra of Mn2p in FA-EM@MnO2/ZIF-8/ICG (a) and FA-EM@MnO2/ZIF-8/ICG in TME-simulating solution (b). Fig. S2. Viability of GES-1 cells (a) and 4T1cells (b) upon treated with PBS (1), PBS + NIR (2), ZIF-8/ICG + NIR (3), MnO2/ZIF-8/ICG + NIR (4), and FA-EM@MnO2/ZIF-8/ICG + NIR (5) by flow-cytometry. Fig. S3. Flow-cytometry analyzed 4T1 and GES-1 cells uptake behavior for FA-EM@MnO2/ZIF-8/ICG nano-platform. Fig. S4. Flow-cytometry analyzed macrophage cells phagocytosis behavior...

Additional file 1 of Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Shanshan Jiang, Yue Wang, Qin Liu, Qinqin Zhao, Liwei Gao, Xin Song, Xuezhi Li, Yinbo Qu & Guodong Liu
Additional file 1: Table S1. Primers used for strain construction and verification in this study. Table S2. Primers used for qPCR in this study. Table S3. Copy numbers of poman5A gene in the constructed strains measured by qPCR. Figure S1. Map of plasmid pM5p.

An optimized exosome production strategy for enhanced yield while without sacrificing cargo loading efficiency

Rongxin Zhang, Te Bu, Ruidan Cao, Zhelong Li, Chen Wang, Bing Huang, Mengying Wei, Lijun Yuan & Guodong Yang
Abstract Background Exosome mediated mRNA delivery is a promising strategy for the treatment of multiple diseases. However, the low yield of exosomes is a bottleneck for clinical translation. In this study, we boosted exosome production via simultaneously reducing the expression of genes inhibiting exosome biogenesis and supplementing the culture medium with red cell membrane components. Results Among the candidate genes, knocking down of Rab4 was identified to have the highest efficacy in promoting exosome biogenesis...

Additional file 1 of PKD1 deficiency induces Bronchiectasis in a porcine ADPKD model

Runming Wang, Wenya Li, Haiting Dai, Mingli Zhu, Lingyu Li, Guohui Si, Yilina Bai, Hanyu Wu, Xiaoxiang Hu & Yiming Xing
Supplementary Material 1

Additional file 1 of Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Additional file 1: Table S1. Scores of spontaneous pain-related physical parameters.

Additional file 2 of Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Additional file 2: Table S2. Immunohistochemical reaction protocols.

Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Abstract Background People with spinal cord injury (SCI) frequently develop neuropathic pain (NP) that worsens disability and diminishes rehabilitation efficacy. Chronic NP is presently incurable due to poor understanding of underlying mechanisms. We hypothesized that multilocus neuroinflammation (NIF) might be a driver of SCI NP, and tested it by investigating whether NP coexisted with central NIF, neurotransmission (NTM), neuromodulation (NML) and neuroplasticity (NPL) changes post-SCI. Methods Female Sprague–Dawley rats (230–250 g) with T10 compression or...

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