8,267 Works

Polygonum cuspidatum inhibits the growth of osteosarcoma cells via impeding Akt/ERK/EGFR signaling pathways

Jun Zhao, Boyu Pan, Xinglu Zhou, Chunnuan Wu, Fengcheng Hao, Jie Zhang & Liren Liu
Osteosarcoma (OS), the most prevalent bone malignancy, mainly affects children and adolescents. Despite recent advances in multimodal therapy, the overall survival rate for OS patients remains poor. Chinese herb medicine (CHM) is an alternative therapeutic option for multifaceted diseases such as malignant tumors. For centuries in China, polygonum cuspidatum, a classic CHM, has been used to treat several diseases, nevertheless, the mechanisms underlying its therapeutic effects have not been fully elucidated. Through network pharmacology and...

Synthesis, herbicidal activity study, and molecular docking of novel acylthiourea derivatives

Binbin Jiang, Yunlong Chai, Xu He, Yan Wang, Bo Chen, Yang Li & Ranhong Li
A series of new acylthiourea derivatives were synthesized and characterized. The results of preliminary herbicidal activity tests showed that most compounds had good herbicidal activity against Digitaria adscendens and Amaranthus retroflexus, especially for compounds 4b (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2-chlorophenyl) thiourea) and 4f (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2,3-dichlorophenyl) thiourea). In vivo acetylhydroxylate synthase (AHAS) enzyme activity inhibition tests showed that all compounds had inhibitory activities against the enzyme at a concentration of 100 mg·L−1, with the highest inhibition rate up to 37.05% (compound...

Indocyanine green-loaded exosomes for image-guided glioma nano-therapy

Bo Fan, Song Yang, Yuan-yu Wang, Chao Zhang, Ji-peng Yang, Li-qun Wang, Zhong-qiang Lv, Xue-fang Shi, Zhen-zeng Fan & Jian-kai Yang
Glioblastoma multiforme (GBM) is the most aggressive and deadliest type of brain tumor with a > >th he most aggressive and deadliest type of brain tumor witnear-infrared fluorescent dye, but it is hard to directly target brain tumor. Therefore, our study aimed to develop a new carrier for ICG to accurately visualize cancer tissue during surgery. Exosomes were isolated from culture U-87 MG cells, then loaded with Indocyanine green (ICG) and conjugated with RGERPPR peptide...

Intracellular bacteriolysis contributes to pathogenicity of Staphylococcus aureus by exacerbating AIM2-mediated inflammation and necroptosis

Shiyuan Feng, Yongjun Yang, Zhenzhen Liu, Wei Chen, Chongtao Du, Guiqiu Hu, Shuixing Yu, Peixuan Song & Jinfeng Miao
Staphylococcus aureus can survive within phagocytes. Indeed, we confirm in this study that approximately 10% of population persists in macrophages during S. aureus infection, while the rest are eliminated due to bacteriolysis, which is of particular interest to us. Herein, we observe that the bacteriolysis is an early event accompanied by macrophage death during S. aureus infection. Furthermore, the cell death is significantly accelerated following increased intracellular bacteriolysis, indicating that intracellular bacteriolysis induces cell death....

Ectopic expression of VyCIPK1 gene, isolated from wild grape Vitis yanshanesis J, X. Chen., confers the tolerance to salt in transgenic tobacco

Ziguo Zhu, Lingmin Dai, Guangxia Chen, Guanghui Yu, Xiujie Li, Zhen Han & Bo Li
Calmodulin-like interacting protein kinases play an important role in plant response to abiotic stresses and development. But the role of the CIPK gene in grapevine is unknown. In this study, VyCIPK1, isolated from the Chinese wild grape V. Yanshanesis, was strongly induced by salt stress. Overexpressing VyCIPK1 could induce AOC and AOS, and result in notably increased jamonate levels in tobacco. Under salt stress, transgenic plants showed higher germination rate, leaf number, and fresh weight...

Development of a mass spectrometry-based metabolomics workflow for traceability of wild and cultivated Cordyceps sinensis

Bo Ding, Hanxiang Li, Hongbo Huang, Jianjun Xie, Zhiyuan Wang, Wenrui Chen & Yiwen Tao
Cordyceps sinensis, as an expensive traditional Chinese medicine and edible fungus mycelium, lacks an effective quality evaluation method, especially and cultivated Cordyceps sinensis. In this study, a feasible workflow method was developed for traceability evaluation of wild and cultivated Cordyceps sinensis, based on mass spectrometry-based metabolomics. Mass spectrometry data were firstly acquired from Cordyceps sinensis, samples by liquid chromatography-quadrupole and time of flight mass spectrometry. Characteristic mass spectrometry peaks were extracted by applying the MZmine....

SPRY4 promotes adipogenic differentiation of human mesenchymal stem cells through the MEK–ERK1/2 signaling pathway

Na Li, Yunfei Chen, Haiyan Wang, Jing Li & Robert Chunhua Zhao
Obesity is a chronic metabolic disorder characterized by the accumulation of excess fat in the body. Preventing and controlling obesity by inhibiting the adipogenic differentiation of mesenchymal stem cells (MSCs) and thereby avoiding the increase of white adipose tissue is safe and effective. Recent studies have demonstrated that Sprouty proteins (SPRYs) are involved in cell differentiation and related diseases. However, the role and mechanism of SPRY4 in MSC adipogenic differentiation remain to be explored. Here,...

Molecular movements of trehalose inside a single network enabling a rapidly-recoverable tough hydrogel

Xiaowen Huang, Jimin Fu, Huiyan Tan, Yan Miu, Mengda Xu, Qiuhua Zhao, Yujie Xie, Shengtong Sun, Haimin Yao & Lidong Zhang
It remains a challenge to achieve rapidly recoverable hydrogels by molecular hydrogen-bonding interaction because of its slow interaction kinetics. This work for the first time reports a trehalose (Tre)-based molecular movement mechanism inside a single network of polyacrylamide (PAM) that accelerates the kinetics of hydrogen-bonding interaction, and thereby endows the hydrogel with high toughness and rapid shape and mechanical recoverability. The resultant PAM@Tre hydrogel is capable of full shape recovery after 10,000 loading/unloading cycles at...

A. caviae infection triggers IL-1β secretion through activating NLRP3 inflammasome mediated by NF-κB signaling pathway partly in a TLR2 dependent manner

Qiankun Yang, Jianguo Zhang, Feixue Liu, Huizhen Chen, Wei Zhang, Haitao Yang, Nana He, Jingquan Dong & Panpan Zhao
Aeromonas caviae, an important food-borne pathogen, induces serious invasive infections and inflammation. The pro-inflammatory IL-1β functions against pathogenic infections and is elevated in various Aeromonas infection cases. However, the molecular mechanism of A. caviae-mediated IL-1β secretion remains unknown. In this study, mouse macrophages (PMs) were used to establish A. caviae infection model and multiple strategies were utilized to explore the mechanism of IL-1β secretion. IL-1β was elevated in A. caviae infected murine serum, PMs lysates...

Improving the performance of DSSCs by modulating the electron donor and electron acceptor of dye molecules with the DTPBT group as π-bridge

Shihan Zhao, Qian Liu & Jiameng Cao
When searching for abundant and economically viable green energy sources is a pressing task of our time, the study of dye-sensitized solar cells (DSSCs) as a photovoltaic conversion device is particularly important. Thus, to improve the performance of dye molecules with large planar dithienopyrrolobenzothiadiazole (DTPBT) as a π-bridge, the electron donor (D) and electron acceptor (A) of the H1 molecule were replaced with phenanthroimidazole and rhodanine 3-acetic acid, respectively, resulting in the new molecules Q1...

CircCLTH promotes skeletal muscle development and regeneration

Mengjie Chen, Qingyou Liu, Mingming Song, Xingyu Liu, Kongwei Huang, Dandan Zhong, Yaling Chen, Mingsheng Jiang, Junming Sun, Yiqiang Ouyang, Suren R Sooranna, Deshun Shi & Hui Li
Buffalo holds an excellent potential for beef production, and circRNA plays an important role in regulating myogenesis. However, the regulatory mechanism of circRNAs during buffalo skeletal muscle development has not been fully explored. In this study, circRNA expression profiles during the proliferation and differentiation stages of buffalo myoblasts were analysed by RNA-seq. Here, a total of 3,142 circRNAs candidates were identified, and 110 of them were found to be differentially expressed in the proliferation and...

Placental DNA methylation changes in gestational diabetes mellitus

Fujia Chen, Xiaoping Fei, Weiqiang Zhu, Zhaofeng Zhang, Yupei Shen, Yanyan Mao, Qianxi Zhu, Jianhua Xu, Weijin Zhou, Min Li & Jing Du
In this study, we investigated the association between altered methylation in the maternal placenta and hyperglycaemia and explored the epigenetic mechanisms underlying gestational diabetes mellitus (GDM). Reduced representation bisulphite sequencing (RRBS) and RNA sequencing (RNA-seq) were performed on placental tissues obtained from women with GDM and healthy controls. Further, pyrosequencing, correlation analyses, and linear regression analyses were performed to valuate relationships between aberrantly methylated-differentially expressed genes and clinical parameters. The EMBOSS and JASPAR databases were...

Placental DNA methylation changes in gestational diabetes mellitus

Fujia Chen, Xiaoping Fei, Weiqiang Zhu, Zhaofeng Zhang, Yupei Shen, Yanyan Mao, Qianxi Zhu, Jianhua Xu, Weijin Zhou, Min Li & Jing Du
In this study, we investigated the association between altered methylation in the maternal placenta and hyperglycaemia and explored the epigenetic mechanisms underlying gestational diabetes mellitus (GDM). Reduced representation bisulphite sequencing (RRBS) and RNA sequencing (RNA-seq) were performed on placental tissues obtained from women with GDM and healthy controls. Further, pyrosequencing, correlation analyses, and linear regression analyses were performed to valuate relationships between aberrantly methylated-differentially expressed genes and clinical parameters. The EMBOSS and JASPAR databases were...

Usefulness of Triglyceride-glucose index for detecting prevalent atrial fibrillation in a type 2 diabetic population

Wenrui Shi, Mu Qin, Shaohui Wu, Kai Xu, Qidong Zheng & Xu Liu
Atrial fibrillation (AF) is the most common arrhythmias, which significantly jeopardizes global cardiovascular health through the complicated heart failure and stroke. Published studies have demonstrated the impact of insulin resistance on the genesis of AF. Hence, monitoring insulin resistance may be a possible way to improve the detection of early-stage AF. Accordingly, our work aimed to investigate the association between TyG, a surrogate of insulin resistance, and the prevalent AF, and to evaluate the potential...

Genome-wide identification, phylogeny and expression analyses of group III WRKY genes in cotton (Gossypium hirsutum)

Lijun Dong, Meng Wang, Xue Zhang, Jianfeng Liu & Shuling Zhang
Cotton (Gossypium hirsutum) is an important economic crop, so it is of great significance to analyze its molecular mechanisms of resistance to Verticillium Wilt. The WRKY gene family encodes transcription factors (TFs) involved in plant resistance to biotic and abiotic stress. Here, we identified 17 group III WRKY genes in cotton based on the cotton genome project. The phylogenetic relationship and evolutionary of WRKY Group III TFs in Arabidopsis thaliana and G. hirsutum showed that...

Additional file 1 of A CT-based nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodule according to the 2021 WHO classification

Qilong Song, Biao Song, Xiaohu Li, Bin Wang, Yuan Li, Wu Chen, Zhaohua Wang, Xu Wang, Yongqiang Yu, Xuhong Min & Dongchun Ma
Additional file 1: Supplemental Table 1. The ICC values of quantitative parameters between three radiologists.

Additional file 2 of A CT-based nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodule according to the 2021 WHO classification

Qilong Song, Biao Song, Xiaohu Li, Bin Wang, Yuan Li, Wu Chen, Zhaohua Wang, Xu Wang, Yongqiang Yu, Xuhong Min & Dongchun Ma
Additional file 2: Supplemental Table 2. The kappa coefficients of categorical variables between three radiologists.

Identification and quantification of anthocyanins of 62 blueberry cultivars via UPLC-MS

Silu Wang, Bowei Wang, Kun Dong, Jing Li, Yadong Li & Haiyue Sun
Blueberries are rich in anthocyanins, which are plant-specific secondary metabolites that are a good source of plant nutrients. Although anthocyanins have been investigated, there is a lack of comprehensive detailed research that includes large numbers of blueberry cultivars, and the anthocyanin compositions of some cultivars are still unknown. Consequently, we determined the anthocyanin compositions of 62 cultivars using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). In total, we identified 30 anthocyanins derived from five kinds of anthocyanidin....

A magnetophoretic microdevice for multi-magnetic particles separation based on size: a numerical simulation study

Jia Ruan, Weiwei Zhang, Chi Zhang, Na Li, Jian Jiang & Huilan Su
Due to the outstanding properties, magnetic particles have been widely used as magnetic carriers and adsorbents in a diverse range of fields, such as biomedical diagnostics, food safety, and environmental monitoring, etc. Sorting different magnetic particles from each other based on size simultaneously can be very useful in multiple targets separation. To the best of our knowledge, this research firstly proposes the arc comb structure microfluidic (ACSM) chip for multi-magnetic particles separation based on size...

Bioinformatics analysis deciphering the transcriptomic signatures associated with signalling pathways and prognosis in the myelodysplastic syndromes

Niluopaer Tuerxun, Jie Wang, Fang Zhao, Yu-ting Qin, Huan Wang, Rong Chen & Jian-ping Hao
Background: Several studies scatteredly identified the myelodysplastic syndromes’ transcriptomic profiles (MDS). However, the exploration of transcriptional signatures, key signalling pathways, and their association with prognosis and diagnosis in the integrated multiple datasets remains lacking. Methods: We integrated the GSE4619, GSE19429, GSE30195, and GSE58831 microarray datasets of CD34 + cells for identifying the differentially expressed genes (DEGs) in the MDS. The series of bioinformatics methods are applied to identify the key hub genes, gene clusters, prognostic...

Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer

Di Xu, Wen-Juan Tang, Yi-Zhi Zhu, Zhen Liu, Kai Yang, Ming-Xing Liang, Xiu Chen, Yang Wu, Jin-Hai Tang & Wei Zhang
To investigate the mechanism through which hyperthermia promotes exosome secretion and drug sensitivity in adriamycin-resistant breast cancer. We first evaluated the effect of hyperthermia on adriamycin-resistant breast cancer viability and used transmission electron microscopy, nanoparticle tracking analysis, and a bicinchoninic acid kit to validate the effect of hyperthermia on exosome secretion. The effective targeting molecules and pathways changed by hyperthermia were explored by RNA microarray and verified in vitro. The adriamycin-resistant MCF-7/ADR cells co-incubated with...

Nutritional components and protein quality analysis of genetically modified phytase maize

Yichun Hu, Liqin Linghu, Min Li, Deqian Mao, Yu Zhang, Xiaoguang Yang & Lichen Yang
The nutritional components and protein quality of genetically modified maize expressing phytase gene (GM) were analyzed and evaluated in this study. The nutritional components were analyzed by Chinese national standard methods. The ileostomy Bama miniature pigs were utilized to analyze the true digestibility of protein and amino acids. The digestible indispensable amino acid score (DIAAS) was adopted to evaluate the protein quality of GM, its parental maize (PM) and commercial available maize Zhengdan 958 (ZD)....

LncRNA miR-17-92a-1 cluster host gene (MIR17HG) promotes neuronal damage and microglial activation by targeting the microRNA-153-3p/alpha-synuclein axis in Parkinson’s disease

Jianzhong Zhang, Yun Yang, Chaoyang Zhou, Ronglan Zhu, Xiang Xiao, Bin Zhou & Dengfeng Wan
Long noncoding RNAs (lncRNAs) have been regarded as modulators of neurodegenerative diseases. Here, we addressed the role of lncRNA miR-17-92a-1 cluster host gene (MIR17HG) in Parkinson’s disease (PD). C57BL/6 mice and SH-SY5Y cells were intervened with 6-hydroxydopamine (6-OHDA) to set up PD models in vivo and in vitro. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was implemented to compare the expression of MIR17HG and miR-153-3p. Cell viability and apoptosis were estimated by 3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and...

MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules

Zhaoyi Pan, Yujie Feng, Zhihui Wang, Zhengyang Lei, Qiuju Han & Jian Zhang
MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration through its endonuclease activity. Transcriptome sequencing revealed that MERS-CoV nsp1 specifically downregulated the mRNAs of ribosomal protein genes, oxidative phosphorylation protein genes, and antigen presentation genes, but upregulated the mRNAs of transcriptional regulatory genes. Further analysis shown nsp1 existed in a...

Comprehensive analysis of peripheral blood non-coding RNAs identifies a diagnostic panel for fungal infection after transplantation

Anli Yang, Huadi Chen, Jianwei Lin, Ming Han, Xiaopeng Yuan, Tao Zhang, Qingwei Nian, Mengran Peng, Dian Li, Chenglin Wu & Xiaoshun He
The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with...

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