Data from: Lessons learned from microsatellite development for non-model organisms using 454 pyrosequencing

Corine Schoebel, Sabine Brodbeck, Dominique Buehler, Carolina Cornejo, Jyoti Gajurel, Hanna Hartikainen, Daniela Keller, Marie Leys, Štěpánka Říčanová, Gernot Segelbacher, Silke Werth, Daniela Csencsics & C. N. Schoebel
Microsatellites, also known as simple sequence repeats (SSRs), are among the most commonly used marker types in evolutionary and ecological studies. Next Generation Sequencing techniques such as 454 pyrosequencing allow the rapid development of microsatellite markers in nonmodel organisms. 454 pyrosequencing is a straightforward approach to develop a high number of microsatellite markers. Therefore, developing microsatellites using 454 pyrosequencing has become the method of choice for marker development. Here, we describe a user friendly way...

Registration Year

  • 2012
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Resource Types

  • Dataset
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Affiliations

  • Swiss Federal Institute of Aquatic Science and Technology
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  • University of Freiburg
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  • Natural History Museum
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  • Swiss Federal Institute for Forest, Snow and Landscape Research
    1
  • Institute of Vertebrate Biology
    1