2 Works

Data from: Successful carnivore identification with faecal DNA across a fragmented Amazonian landscape

Fernanda Michalski, Fernanda Pedone Valdez, Darren Norris, Chris Zieminski, Cyntia Kayo Kashivakura, Cristine S. Trinca, Heath B. Smith, Carly Vynne, Samuel K. Wasser, Jean Paul Metzger & Eduardo Eizirik
The use of scat surveys to obtain DNA has been well documented in temperate areas, where DNA preservation may be more effective than in tropical forests. Samples obtained in the tropics are often exposed to high humidity, warm temperatures, frequent rain, and intense sunlight, all of which can rapidly degrade DNA. Despite these potential problems, we demonstrate successful DNA amplification and sequencing for faeces of carnivores collected in tropical conditions and quantify how sample condition...

Data from: DNA barcoding meets molecular scatology: short mtDNA sequences for standardized species assignment of carnivore noninvasive samples

Paulo B. Chaves, Vanessa G. Graeff, Marília B. Lion, Larissa R. Oliveira & Eduardo Eizirik
Although species assignment of scats is important to study carnivoran biology, there is still no standardized assay for the identification of carnivores worldwide, which would allow large-scale routine assessments and reliable cross-comparison of results. Here we evaluate the potential of two short mtDNA fragments (ATP6 [126 bp] and COI [187 bp]) to serve as standard markers for the Carnivora. Samples of 66 species were sequenced for one or both of these segments. Alignments were complemented...

Registration Year

  • 2011
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Resource Types

  • Dataset
    2

Affiliations

  • Pontifical Catholic University of Rio Grande do Sul
    2
  • Sao Paulo State University
    1
  • University of Washington
    1
  • University of Brasília
    1
  • University of Sao Paulo
    1
  • University of Calgary
    1
  • Universidade do Vale do Rio dos Sinos
    1