Zaire ebolavirus (EBOV) causes a severe hemorrhagic fever in humans and non-human primates with high morbidity and mortality. EBOV infection is dependent on its structural glycoprotein (GP), but high levels of GP expression also trigger cell rounding, detachment, and downregulation of many surface molecules that is thought to contribute to its high pathogenicity. Thus, EBOV has evolved an RNA editing mechanism to reduce its GP expression and increase its fitness. We now report that the...

The low OH−/RE3+ molar ratio of hydroxide sulfate RE(OH)SO4 (RE = rare earth) makes it a potential good template for the synthesis of rare earth compounds, which is shown in this work by the direct generation of (La,Eu)F3, (La,Eu)PO4, Na(La,Eu)(WO4)2, and Na(La,Eu)(MoO4)2 without further calcination. The template showed irregular and slightly aggregated micro-plates morphology, while the products derived from the template showed morphologies of nanoparticles (trifluoride), nanorods (phosphate), aggregate spheres (molybdate), and aggregated polyhedrons (tungstate)....

The purpose of this paper is to study the behavior characteristics and spatiotemporal distribution of groups’ multi-day leisure travel, and to quantify the impact of the elderly and children on group travel choices. Based on questionnaire and social media data, three choice models and an agent-based simulation model were established to explore the micro-choice behavior characteristics and the macro-spatiotemporal distribution of groups’ multi-day leisure travel by arranging the travel rules and by simulating the route...

The late Paleozoic tectonic evolution of the Lhasa terrane and subduction of Sumdo Paleo-Tethys ocean remains controversial due to limited data and variable Permian magmatism in central and southern Lhasa. We report new zircon U–Pb ages and Hf-isotope ratios as well as whole-rock major element, trace element, and Sr–Nd isotope data from middle Permian (266 ~ 257 Ma) arc-related magmatism, including high-Mg quartz diorite and trondhjemite in the Tangjia-Sumdo area. The quartz diorite has high...

The number and quality of oocytes in the ovarian reserve are related to fertility and reproductive lifespan in mammals. Some transcription factors have been demonstrated to determine oogenesis. The insulinoma-associated 2 (Insm2) gene is a member of the Snail transcriptional repressor superfamily. Recent studies have demonstrated Insm2 plays an essential role for insulin secretion and glucose intolerance in mice, but the functions of Insm2 in reproduction remain elusive. Here, by examination of Insm2 knockout mice,...

Aminoglycosides exhibit ototoxicity by damaging mitochondria, which in turn generate reactive oxygen species that induce hair cell death and subsequent hearing loss. It is well known that damaged mitochondria are degraded by mitophagy, an important mitochondrial quality control system that maintains mitochondrial homeostasis and ensures cell survival. However, it is unclear whether dysregulation of mitophagy contributes to aminoglycoside-induced hair cell injury. In the current study, we found that PINK1-PRKN-mediated mitophagy was impaired in neomycin-treated hair...

Collaborative geographic analysis can lead to better outcomes but requires complicated interactions among participants, support resources and analytic tools. A process expression with explicit structure and content can help coordinate and guide these interactions. For different geographic problems, the structure and content of collaborative geographic analysis are generally distinct. Since the process structure embodies the pathway of problem-solving and the process content contains the information flow and internal interactions, both the structure and the content...

Accumulating evidence has unfolded the significance of extracellular vesicles (EVs) in diseases and cancers. Here, we attempted to discuss the role of cancer-associated fibroblasts (CAFs)-derived EVs containing miR-199a-5p in gastric tumorigenesis. Upregulated miR-199a-5p was first identified in cancer cells. Then, we selected CAFs for isolation of EVs which were co-cultured with AGS cells. We observed successful delivery of miR-199a-5p via CAF-derived EVs. Besides, miR-199a-5p promoted malignant properties of AGS cells. Moreover, miR-199a-5p downregulated FKBP5, leading...

Additional file 3: Supplement Fig. 3. Graph of sensitivity analysis.

Supplemental Document

Abstract Purpose To establish a nomogram for predicting the risk of adenocarcinomas in patients with subsolid nodules (SSNs) according to the 2021 WHO classification. Methods A total of 656 patients who underwent SSNs resection were retrospectively enrolled. Among them, 407 patients were assigned to the derivation cohort and 249 patients were assigned to the validation cohort. Univariate and multi-variate logistic regression algorithms were utilized to identity independent risk factors of adenocarcinomas. A nomogram based on...

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

The aim of the study was to determine molecular genetic and clinical characterization of acute myeloid leukemia (AML) with trisomy 8 as the sole chromosome abnormality, a recurrent but rare chromosomal abnormality in AML. Interphase fluorescence in situ hybridization, reverse transcriptase-quantitative polymerase chain reaction for gene rearrangement and next-generation sequencing (NGS) were performed on sole trisomy 8 AML patients. A total of 35 AML patients with trisomy 8 as the sole chromosome abnormality were screened....

Additional file 1: Primer sequences for qRT-PCR.

Additional file 1.

Additional file 3: Table S2.Comparison of SHERRY and SHERRY2 performances on bulk RNA. (recording data values of Additional file 1: Fig. S2).

Additional file 1: Fig. S1. Conditions for bulk RNA. Fig. S2. Improved performance of SHERRY2 on bulk RNA. Fig. S3. Conditions for single cells. Fig. S4. Accuracy of SHERRY2 for single cells. Fig. S5. Flow cytometry gating of single lymphocytes and single GC B cells. Fig. S6. DNase activity and performance in SHERRY2 library construction. Fig. S7. GC information gained from SHERRY2. Fig. S8. BCR sequences of single GC B cells identified by SHERRY2. Fig....

Additional file 3: Table S2.Comparison of SHERRY and SHERRY2 performances on bulk RNA. (recording data values of Additional file 1: Fig. S2).

In this work, an efficient synthesis of 3-hydroxy chromones was accomplished through oxidative cyclization mediated by lipase. This enzymatic process has salient features of environmental friendliness, mild reaction condition, satisfactory yield, green reusable biocatalyst, and short reaction time. The proposed method expands the application of lipase in organic synthesis.

Additional file 1: The results of next generation sequencing of cerebrospinal fluid.

Additional file 2: Comparison of the etiological results between conventional detection methods and next-generation sequencing technology.

Additional file2. Table S1. Metals, soluble inorganic ions, polycyclic aromatic hydrocarbons (PAHs) and carbon in the PM2.5 samples. Table S2. Anatomic data for filter air and long-term PM2.5-exposed mice. Table S3. Anatomic data for control and acute PM2.5-exposed mice. Table S4. Anatomic data for wild type and Ddah1−/− mice after PM2.5 exposure. Table S5. Anatomic data for wild type and DDAH1-TG mice after PM2.5 exposure. Table S6. Detail information for antibodies. Table S7. The quantitative...

Additional file 11. Supplementary methods.

Additional file 1. The mass spectromy data of the band at 70kDa of circZNF451 RNA pulldown in macrophages.

Additional file 1: Table S1. OSCE criteria scores of CPR for stage assessment in standardized training.