Supplemental material, sj-png-1-nnr-10.1177_15459683231159662 for Cerebrospinal Fluid Pressure Dynamics as a Bedside Test in Traumatic Spinal Cord Injury to Assess Surgical Spinal Cord Decompression: Safety, Feasibility, and Proof-of-Concept by Najmeh Kheram, Andrea Boraschi, Nikolai Pfender, Susanne Friedl, Maria Rasenack, Benjamin Fritz, Vartan Kurtcuoglu, Martin Schubert, Armin Curt and Carl M. Zipser in Neurorehabilitation and Neural Repair

Additional file 1. Supplementary Figures.

Additional file 2. Reviewer reports.

Additional file 1: Supplemental Table S1. Scoring standards for the disease activity index (DAI)1,2. Supplemental Table S2. Parameters and criteria of histological damage evaluation1,2. Supplemental Table S3. Primer pairs used in this experiment1. Supplemental Figure S1. Fecal bile acids (BAs) profiles of low birth weight (LBW) and normal birth weight (NBW) piglets 2 days after birth (n = 10). Supplemental Figure S2. Verification of depletion of the intestinal microbiota in mice (n = 8). Supplemental...

Abstract Introduction Neuropsychiatric symptoms are important treatment targets in the management of dementia and can be present at very early clinical stages of neurodegenerative diseases. Increased cortisol has been reported in Alzheimer’s disease (AD) and has been associated with faster cognitive decline. Elevated cortisol output has been observed in relation to perceived stress, depression, and anxiety. Dehydroepiandrosterone sulfate (DHEAS) has known anti-glucocorticoid effects and may counter the effects of cortisol. Objectives We aimed to examine...

Additional file 1. Clinical information of all patients in research.

Additional file 2. Supplementary figures and tables.

Additional file 3. List of peak of RIP-seq and KEGG analysis of peak-related genes.

Abstract Background With increasing incidence and mortality, colorectal cancer (CRC) seriously endangers human health. LARP6, a member of La-related protein (LARP) family, is a RNA binding protein and probably associates with CRC progression, but its specific roles and mechanisms in CRC still remain unknown. Method Quantitative real-time PCR (qPCR), western blot, and immunohistochemistry were employed to examine LARP6 expression in CRC tissues. Using the stable LARP6 overexpression or interference CRC cell lines, the effect of...

Additional file 1: Supplementary Text. Tables S1-2 (reference datasets), Table S3 (quality control summaries), and Table S4 (method parameters); description of evaluation statistics (for 1/2D comparisons, clustering and integration), computational Snakemake workflow, and supplementary R data objects.

Additional file 1: Supplementary Text. Tables S1-2 (reference datasets), Table S3 (quality control summaries), and Table S4 (method parameters); description of evaluation statistics (for 1/2D comparisons, clustering and integration), computational Snakemake workflow, and supplementary R data objects.

Additional file 2: Figures S1-3 (t-SNEs of reference datasets), Figs. S4-10 (comparison of 1/2D test statistics), Figs. S11-13 (runtime and memory usage), Figs. S14-16. (integration results), Figs. S17-24 (t-SNEs of integrated datasets), Figs. S25-28 (meta-analysis of summaries).

Additional file 3. Review History.

Additional file 1: Table S1.

Supplementary Material 2. Material

Additional file 2: Fig. S2. Correlation between N2O emission, hyphal length density and nosZ gene copies or transcript copies. A, pot expt 1. Correlation between N2O concentration and nosZ gene copies in different patch types 24 h after the addition of ammonium or nitrate. B, pot expt 1. Correlation between nosZ gene copies and hyphal length density in different patche types. Control, soil patch; NSfaba and Sfaba, patches with unsterilized (NS) or sterilized (S) faba...

Additional file 1: Fig. S1. Hyphal length density (pot expt 1), patch N2O concentrations (pot expt 1) dissolved organic carbon content, and total carbon and nitrogen contents (pot expt 2) in patches in the absence or presence of AMF. A, pot expt 1. Hyphal length density from different patches under −AMF and +AMF treatments (n = 8). B, pot expt1. Dynamic N2O concentrations from different patches under −AMF and +AMF treatments after the addition of...

Additional file 1. Supplementary figures and tables

Abstract Background During the 2020/2021 winter, the labour market was under the impact of the COVID-19 pandemic. Changes in socioeconomic resources during this period could have influenced individual mental health. This association may have been mitigated or exacerbated by subjective risk perceptions, such as perceived risk of getting infected with SARS-CoV-2 or perception of the national economic situation. Therefore, we aimed to determine if changes in financial resources and employment situation during and after the...

Additional file 4: Supplementary Figure 4. Analysis of viable CD3+, CD4+ and CD8+ cells, referred to live CD45+ cells, in lung tumors from mice receiving different in vivo treatments. *p<0.05, **p<0.02, ***p<0.01.

Additional file 8: Materials and Methods. Supplementary Text. Table S1. Temporal N2O concentrations (μL L-1) in the headspace in the preliminary experiment. Table S2. Primers and PCR conditions used for the PCR. Table S3. Stepwise multiple regression to identify the abundance and expression of key genes involved in N cycling which had the strongest statistical contributions to variation in the cumulative N2O emission in pot expt 2. Independent variables include the abundances and expressions of...

Additional file 1. Morphological characterization of CNS-resident myeloid cells following ablation of IGF-1R.To assess the morphology of CNS resident microglia (brain and spinal cord) and leptomeningeal BAMs in IGF-1RKO-tdTomato (n=4 mice) compared to IGF-1RWT-tdTomato (n=4 mice), we performed Sholl analysis, skeletal analysis and fractal-lacunarity analysis (see methods for more details). The parameters obtained from these 3 morphological analyses were grouped into 4 categories indicating cell size (comprising quantification of soma size, cell density, critical value...

Abstract Signaling by insulin-like growth factor-1 (IGF-1) is essential for the development of the central nervous system (CNS) and regulates neuronal survival and myelination in the adult CNS. In neuroinflammatory conditions including multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE), IGF-1 can regulate cellular survival and activation in a context-dependent and cell-specific manner. Notwithstanding its importance, the functional outcome of IGF-1 signaling in microglia/macrophages, which maintain CNS homeostasis and regulate neuroinflammation, remains...

Additional file 1: Supplementary Figure 1. Abundance plots of the gut microbiota in pregnancy at least 20 weeks gestational age stratified by HIV status. (A) abundance plot at phylum level (B) abundance plot at genus level.

Abstract Background Human immunodeficiency virus (HIV) severely damages the epithelial cells of the gut lining leading to an inflamed leaky gut, translocation of microbial products, and dysbiosis resulting in systemic immune activation. Also, microbiota composition and maternal gut function can be altered in pregnancy through changes in the immune system and intestinal physiology. The aim of this study was to investigate the gut microbiota in HIV-infected and HIV-uninfected pregnant women and to compare and identify...