57 Works

The mechanism underlying transient weakness in myotonia congenita

Jessica Myers, Mark Rich, Kirsten Denman, Chris DuPont, Ahmed Hawash, Kevin Novak, Andrew Koesters, Manfred Grabner, Anamika Dayal & Andrew Voss
In addition to the hallmark muscle stiffness, patients with recessive myotonia congenita (Becker disease) experience debilitating bouts of transient weakness that remain poorly understood despite years of study. We made intracellular recordings from muscle of both genetic and pharmacologic mouse models of Becker disease to identify the mechanism underlying transient weakness. Our recordings reveal transient depolarizations (plateau potentials) of the membrane potential to -25 to -35 mV in the genetic and pharmacologic models of Becker...

Multiple mutualism effects generate synergistic selection and strengthen fitness alignment in the interaction between legumes, rhizobia, and mycorrhizal fungi

Michelle Afkhami, Maren Friesen & John Stinchcombe
Nearly all organisms participate in multiple mutualisms, and complementarity within these complex interactions can result in synergistic fitness effects. However, it remains largely untested how multiple mutualisms impact eco-evolutionary dynamics in interacting species. We tested how multiple microbial mutualists-- N-fixing bacteria and mycorrrhizal fungi-- affected selection and heritability of traits in their shared host plant (Medicago truncatula), as well as fitness alignment between partners. Our results demonstrate for the first time that multiple mutualisms synergistically...

Effectiveness and feasibility of convalescent blood transfusion to reduce COVID-19 fatality ratio

Xi Huo, Xiaodan Sun, Nicola Bragazzi & Jianhong Wu
As of December 2020, COVID-19 has spread all over the world with more than 81 million cases and more than 1.8 million deaths. The rapidly increasing number of patients mandates the consideration of potential treatments for patients under severe and critical conditions. Convalescent plasma (CP) treatment refers to the approach of infusing patients with plasma from recently recovered patients. CP appears to be a possible therapeutic option to manage patients suffering from severe or even...

Construction of a novel risk model based on the random forest algorithm to distinguish pancreatic cancers with different prognoses and immune microenvironment features

Yalan Lei, Rong Tang, Jin Xu, Bo Zhang, Jiang Liu, Chen Liang, Qingcai Meng, Jie Hua, Xianjun Yu, Wei Wang & Si Shi
Immune-related long noncoding RNAs (irlncRNAs) are actively involved in regulating the immune status. This study aimed to establish a risk model of irlncRNAs and further investigate the roles of irlncRNAs in predicting prognosis and the immune landscape in pancreatic cancer. The transcriptome profiles and clinical information of 176 pancreatic cancer patients were retrieved from The Cancer Genome Atlas (TCGA). Immune-related genes (irgenes) downloaded from ImmPort were used to screen 1903 immune-related lncRNAs (irlncRNAs) using Pearson’s...

Effects of cucurbituril size on the binding of a lutidine guest

Angel E. Kaifer, Vladimir Sindelar & Jan Svec
Six- and seven-membered cucurbiturils form inclusion complexes with a lutidine derivative. These inclusion complexes differ in the binding mode, stability, kinetics of complexation, and sensitivity toward basic media.

Terminal Carboxylate Effects on the Thermodynamics and Kinetics of Cucurbit[7]uril Binding to Guests Containing a Central Bis(Pyridinium)-Xylylene Site

Iago Neira, Marcos D. Garcia, Carlos Peinador & Angel Kaifer
A series of bis(pyridinium)-xylylene derivatives bearing carboxylate terminal groups were investigated as guests for the cucurbit[7]uril host in aqueous solution. While the presence of the terminal carboxylates has a modest effect on the thermodynamic stability of the complexes, the kinetics of complex association/dissociation is strongly affected. The relative position (meta, para) of the carboxylate group in relation to the pyridinium nitrogen also exerts a considerable effect on the binding kinetics.

Area and Timing data and R script for: 3D scanning as a tool to measure growth rates of live coral microfragments used for coral reef restoration

Hanna Koch, Bailey Wallace, Allyson DeMerlis, Abigail Clark & Robert Nowicki
Rapid and widespread declines in coral health and abundance have driven increased investments in coral reef restoration interventions to jumpstart population recovery. Microfragmentation, an asexual propagation technique, is used to produce large numbers of corals for research and restoration. As part of resilience-based restoration, coral microfragments of different genotypes and species are exposed to various stressors to identify candidates for propagation. Growth rate is one of several important fitness-related traits commonly used in candidate selection,...

Ecological and biogeographic processes drive the proteome evolution of snake venom

Tuany Siqueira-Silva, Luiz Lima, Jônatas Chaves-Silveira, Talita Amado, Julian Naipauer, Pablo Riul & Pablo Martinez
The emergence of venom is an evolutionary innovation that favored the diversification and survival of snakes. The composition of snake venoms is known in detail from venom gland proteomic data. However, there is still a gap of knowledge about the forces that lead to the expression of different toxins in different proportions in the venom cocktail across space and time. LOCATION World. TIME PERIOD Modern. MAJOR TAXA STUDIED Elapidae and Viperidae. We integrated proteomic data...

Aboveground carbon stocks and dynamics in Andean forests

Alvaro Duque, Miguel Peña, Francisco Cuesta, Sebastián González-Caro, Peter Kennedy, Oliver Phillips, Marco Calderón, Cecilia Blundo, Julieta Carilla, Leslie Cayola, William Farfán-Ríos, Alfredo Fuentes, Ricardo Grau, Jürgen Homeier, María I. Loza-Rivera, Jonathan A. Myers, Oriana Osinaga-Acosta, Manuel Peralvo, Esteban Pinto, Sassan Saatchi, Miles Silman, J. Sebastián Tello, Andrea Terán-Valdez & Kenneth J. Feeley
This dataset (Andean_AGB.xlsx) has the data employed in the paper entitled Old-growth Andean forests as globally important carbon sinks and future carbon refuges. The data was compiled as the results of the work of several research teams spread out across the Andean region. The information available here has data about aboveground carbon stocks and dynamics and the main explanatory variables, such as climate and symbiotic root associations.

Data from: Positive allosteric modulation of the α7 nicotinic acetylcholine receptor as a treatment for cognitive deficits after traumatic brain injury

David J. Titus, Timothy Johnstone, Nathan H. Johnson, Sidney H. London, Meghana Chapalamadugu, Derk Hogenkamp, Kelvin W. Gee & Coleen M. Atkins
Cognitive impairments are a common consequence of traumatic brain injury (TBI). The hippocampus is a subcortical structure that plays a key role in the formation of declarative memories and is highly vulnerable to TBI. The α7 nicotinic acetylcholine receptor (nAChR) is highly expressed in the hippocampus and reduced expression and function of this receptor are linked with cognitive impairments in Alzheimer’s disease and schizophrenia. Positive allosteric modulation of α7 nAChRs with AVL-3288 enhances receptor currents...

Comparison of adult census size and effective population size support the need for continued protection of two Solomon Island endemics

Sarah A. Cowles, Brian C. Weeks, Lindsey Perrin, Nancy Chen & J. Albert C. Uy
Because a population’s ability to respond to rapid change is dictated by standing genetic variation, we can better predict a population’s long-term viability by estimating and then comparing adult census size (N) and effective population size (Ne). However, most studies only measure N or Ne, which can be misleading. Using a combination of field and genomic sequence data, we here estimate and compare N and Ne in two range-restricted endemics of the Solomon Islands. Two...

Interindividual plasticity in metabolic and thermal tolerance traits from populations subjected to recent anthropogenic heating

Melissa Drown, Amanda DeLiberto, Moritz Ehrlich, Douglas Crawford & Marjorie Oleksiak
To better understand temperature’s role in the interaction between local evolutionary adaptation and physiological plasticity, we investigated acclimation effects on metabolic performance and thermal tolerance among natural Fundulus heteroclitus populations from different thermal environments. F. heteroclitus populations experience large daily and seasonal temperature variations, as well as local mean temperature differences across their large geographic cline. In this study, we focus on three populations: one locally heated (32°C) by thermal effluence (TE) from the Oyster...

Diversity Residency Toolkit

Kalani Adolpho, Maya Bergamasco, Ana Corral, , , & Liburd Tavernier W

Ensemble and calibration multiply robust estimation for quantile treatment effect

Xiaohong He & Lei Wang
Quantile treatment effects can be important causal estimands in the evaluation of biomedical treatments or interventions for health outcomes such as birthweight and medical cost. However, the existing estimators require either a propensity score model or a conditional density vector model is correctly specified, which is difficult to verify in practice. In this paper, we allow multiple models for propensity score and conditional density vector, then construct a class of calibration estimators based on multiple...

Multi-locus identification of Psilocybe cubensis by high-resolution melting (HRM)

Xiaochun Zhang, Huan Yu, Ziwei Wang, Qi Yang, Ruocheng Xia, Yiling Qu, Ruiyang Tao, Yan Shi, Ping Xiang, Suhua Zhang & Chengtao Li
Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability...

β-galactosidase GALA from Bacillus circulans with high transgalactosylation activity

Yaru Yan, Weishi Guan, Xiaoyi Li, Kaier Gao, Xinxin Xu, Bo Liu, Wei Zhang & Yuhong Zhang
β-galactosidase catalyzes lactose hydrolysis and transfers reactions to produce prebiotics such as galacto-oligosaccharides (GOS) with potential applications in the food industry and pharmaceuticals. However, there is still a need for improved transgalactosylation activity of β-galactosidases and reaction conditions of GOS production in order to maximize GOS output and reduce production costs. In this study, a β-galactosidase gene, galA, from Bacillus circulans was expressed in Pichia pastoris, which not only hydrolyzed lactose but also had strong...

POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis

Jinze Meng, Hao Wen, Xintong Li, Boyang Luan, Shiqiang Gong, Jie Wen, Yifei Wang & Lei Wang
Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study aimed to identify potential genes for AAA growth and expansion based on comprehensive bioinformatics approaches. Firstly, 29 up-regulated genes were identified through DEGs analysis between large AAA and small AAA in GSE57691. Secondly,...

Construction of a novel risk model based on the random forest algorithm to distinguish pancreatic cancers with different prognoses and immune microenvironment features

Yalan Lei, Rong Tang, Jin Xu, Bo Zhang, Jiang Liu, Chen Liang, Qingcai Meng, Jie Hua, Xianjun Yu, Wei Wang & Si Shi
Immune-related long noncoding RNAs (irlncRNAs) are actively involved in regulating the immune status. This study aimed to establish a risk model of irlncRNAs and further investigate the roles of irlncRNAs in predicting prognosis and the immune landscape in pancreatic cancer. The transcriptome profiles and clinical information of 176 pancreatic cancer patients were retrieved from The Cancer Genome Atlas (TCGA). Immune-related genes (irgenes) downloaded from ImmPort were used to screen 1903 immune-related lncRNAs (irlncRNAs) using Pearson’s...

Tight inclusion complexation of 2,7-dimethyldiazapyrenium in cucurbit[7]uril

Angel E. Kaifer, Vladimir Sindelar, Mabel A. Cejas & Fran�isco M. Raymo
The dicationic guest 2,7-dimethyldiazapyrenium is bound inside the host cucurbit[7]uril, forming a very stable inclusion complex in which the host undergoes structural distortions to accommodate the guest.

Complexation of Ferrocene Derivatives by the Cucurbit[7]uril Host:  A Comparative Study of the Cucurbituril and Cyclodextrin Host Families

Woo Sung Jeon, Kwangyul Moon, Sang Hyun Park, Hyungpil Chun, Young Ho Ko, Jin Yong Lee, Eun Sung Lee, S. Samal, N. Selvapalam, Mikhail V. Rekharsky, Vladimir Sindelar, David Sobransingh, Yoshihisa Inoue, Angel E. Kaifer & Kimoon Kim
The formation of inclusion complexes between cucurbit[7]uril (CB[7]) and ferrocene and its derivatives has been investigated. The X-ray crystal structure of the 1:1 inclusion complex between ferrocene and CB[7] revealed that the guest molecule resides in the host cavity with two different orientations. Inclusion of a set of five water-soluble ferrocene derivatives in CB[7] was investigated by 1H NMR spectroscopy and calorimetric and voltammetric techniques. Our data indicate that all neutral and cationic guests form...

Synthesis and Binding Properties of Monohydroxycucurbit[7]uril: A Key Derivative for the Functionalization of Cucurbituril Hosts

Nan Dong, Jing He, Tao Li, Andrea Peralta, Mehdi Rashvand Avei, Mingfang Ma & Angel E. Kaifer
We present a simple, direct method to prepare monohydroxylated cucurbit[7]uril (CB7-OH) through the direct oxidation of its precursor host, cucurbit[7]uril (CB7). Although the conversion takes place in low yield (14%), the isolation of CB7-OH from the reaction mixture is straightforward, and the unreacted CB7 can be easily recovered. ITC measurements with several selected guests confirmed that CB7-OH binds all of them in aqueous solution with similar, albeit slightly lower, binding affinities than those observed with...

Adaptive introgression of the beta-globin cluster in two Andean waterfowl

Allie Graham & Kevin McCracken
Introgression of alleles has emerged as an important avenue for genetic adaptation in both plant and animal populations. In vertebrates, adaptation to hypoxic high-altitude environments involves the coordination of multiple molecular and cellular mechanisms, including selection on the Hypoxia-Inducible Factor (HIF) pathway and the blood-O2 transport protein hemoglobin (Hb). In two Andean duck species, a striking DNA sequence similarity reflecting identity by descent is present across the ~20 kb b-globin cluster including both embryonic (HBE)...

Transforming Metadata into Linked Data to Improve Digital Collection Discoverability: A CONTENTdm Pilot Project

Greta Bahnemann, Michael J. Carroll, Paul Clough, Mario Einaudi, Chatham Ewing, Jeff Mixter, Jason Roy, Holly Tomren, Bruce Washburn & Elliot Williams

Base-substitution mutation rate across the nuclear genome of Alpheus snapping shrimp and the timing of isolation by the Isthmus of Panama

Katherine Silliman, Jane Indorf, Nancy Knowlton, William Browne & Carla Hurt
The formation of the Isthmus of Panama and final closure of the Central American Seaway (CAS) provides an independent calibration point for examining the rate of DNA substitutions. This vicariant event has been widely used to estimate the substitution rate across mitochondrial genomes and to date evolutionary events in other taxonomic groups. Nuclear sequence data is increasingly being used to complement mitochondrial datasets for phylogenetic and evolutionary investigations; these studies would benefit from information regarding...

The multidimensional (and contrasting) effects of environmental warming on a group of montane tropical lizards: Lizard and plant data

Martha M Munoz, Kenneth Feeley, Patrick Martin & Vincent Farallo
1. Mountains are cradles for biodiversity and crucibles for climate-driven species loss, particularly for tropical ectotherms. Constriction on activity and amplified heat stress are two key sources of warming-driven vulnerability in tropical montane ectotherms. These threats, however, might be counterbalanced if rising temperatures also release organisms from limits on activity induced by cold stress. 2. We used biophysical modeling to estimate activity patterns and thermal stress under warming in a group of summit-dwelling Anolis lizards...

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  • 2021

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  • University of Miami
  • Zhejiang University
  • Sun Yat-sen University
  • Air Force Medical University
  • Fudan University Shanghai Cancer Center
  • Beihang University
  • Sichuan University
  • Fudan University
  • Central South University
  • Huazhong University of Science and Technology