DNA methylation patterns are largely established in-utero and might mediate the impacts of in-utero conditions on later health outcomes. Associations between perinatal DNA methylation marks and pregnancy-related variables, such as maternal age and gestational weight gain, have been earlier studied with methylation microarrays, which typically cover less than 2% of human CpG sites. To detect such associations outside these regions, we chose the bisulphite sequencing approach. We collected and curated clinical data on 200 newborn...

Abstract Objective To compare colorectal cancer (CRC) incidences in carriers of pathogenic variants of the MMR genes in the PLSD and IMRC cohorts, of which only the former included mandatory colonoscopy surveillance for all participants. Methods CRC incidences were calculated in an intervention group comprising a cohort of confirmed carriers of pathogenic or likely pathogenic variants in mismatch repair genes (path_MMR) followed prospectively by the Prospective Lynch Syndrome Database (PLSD). All had colonoscopy surveillance, with...

To review publications exploring non-utilization of oral health services and to identify factors associated with non-utilization of oral health services among children and adolescents. An integrative review design was adopted. A search was conducted for research articles published during the period from 2000 to April 2021 in five databases, Medline via Ovid, Scopus, CINAHL, Cochrane Library and Web of Science. Inclusion criteria were original articles examining non-utilization of oral health services among 0–19 years old...

ObjectivesTo examine trends in the prevalence of dementia and related comorbidities among the oldest old.MethodsSix repeated cross-sectional surveys were conducted between 2001 and 2018, each including all inhabitants aged over 90 in Tampere, Finland (n = 5386). Co-occurring conditions and their time trends among participants with dementia were examined using logistic regression and generalized estimating equations.ResultsThe prevalence of dementia decreased from 47% in 2007 to 41% in 2018. Throughout the study period, depression was more...

Additional file 2: Figure S1. Birth weight percentiles by sex and gestational age for STOPPAM vs. Intergrowth (IG21) references. The percentiles before and after 33 weeks for Intergrowth were merged, hence the bend in the 10th percentile.

Additional file 2: Figure S1. Birth weight percentiles by sex and gestational age for STOPPAM vs. Intergrowth (IG21) references. The percentiles before and after 33 weeks for Intergrowth were merged, hence the bend in the 10th percentile.

Additional file 3: Figure S2. The birth weight z-scores comparing STOPPAM vs. Intergrowth (IG21) references. The dash line indicates the mean birthweight z-scores.

Additional file 4: Figure S3. Association between small for gestational age (SGA) and malaria in pregnancy excluding all HIV seropositive women. Panels A and B shows the unadjusted odds ratio (uOR) for SGA when using STOPPAM (SGASTOPPAM) and Intergrowth-21 (SGAIG21) references. Panels C and D shows the adjusted odds ratio (aOR).

The surgical reconstruction of functional neovagina is challenging and susceptible to complications. Therefore, developing tissue engineering based treatment methods for vaginal defects is important. Our aim was to develop and test a novel supercritical carbon dioxide foamed poly-l-lactide-co-ε-caprolactone (scPLCL) scaffold for vaginal reconstruction. The scaffolds were manufactured and characterized for porosity (65±4%), pore size (350±150µm) and elastic modulus (2.8±0.4MPa). Vaginal epithelial (EC) and stromal cells (SC) were isolated, expanded, and characterized with flow cytometry. Finally,...

The population in many countries is becoming more diverse. The number of people from foreign backgrounds is growing in Finland as well. The aim of this study was to better understand how the foreign background of a patient affects the dentist’s work. The research was carried out as a semi-structured interview. Six dentists from Helsinki municipality public dental care were interviewed between December 2019 and January 2020. After the interviews were transcribed verbatim, two members...

DNA methylation patterns are largely established in-utero and might mediate the impacts of in-utero conditions on later health outcomes. Associations between perinatal DNA methylation marks and pregnancy-related variables, such as maternal age and gestational weight gain, have been earlier studied with methylation microarrays, which typically cover less than 2% of human CpG sites. To detect such associations outside these regions, we chose the bisulphite sequencing approach. We collected and curated clinical data on 200 newborn...

Background Resting heart rate (HR) and its variability (HRV) reflects the cardiac sympathovagal balance that is stimulated by head-up tilting. HRV is influenced by the level of HR, but how much HRV offers additional information about cardiac autonomic tone than HR alone remains unresolved. We examined the relation of resting HR with HRV during head-up tilt. Methods. Hemodynamics of 569 subjects without known cardiovascular diseases and medications with direct cardiovascular effects were recorded using whole-body...

(i) To study the measurement invariance of Orofacial Esthetic Scale (OES) and Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ), (ii) to compare the perception of orofacial appearance (OA) and (iii) to study the frequency of individuals who have sought or received aesthetic dental treatment between Brazil and Finland. This was a cross-sectional observational study with snowball non-probability sample selection. Students and staff from universities in Finland and Brazil were invited to participate. Data were collected...

Abstract Objective To compare colorectal cancer (CRC) incidences in carriers of pathogenic variants of the MMR genes in the PLSD and IMRC cohorts, of which only the former included mandatory colonoscopy surveillance for all participants. Methods CRC incidences were calculated in an intervention group comprising a cohort of confirmed carriers of pathogenic or likely pathogenic variants in mismatch repair genes (path_MMR) followed prospectively by the Prospective Lynch Syndrome Database (PLSD). All had colonoscopy surveillance, with...

Additional file 1.

Supplementary Table 1. This study used 48 DNA methylation datasets, including DILGOM, FTC, ERMA, KORA, LURIC, NELLI, SATSA and YFS as well as 39 datasets available in the Gene Expression Omnibus (GEO) [29] consisting of >30 tissues and >30,000 individuals.
Supplementary Table 2. Differences in the proportion of individuals with imprinted nc886 locus between sexes or in a case–control setting.
Supplementary Table 3. Of these discordant pairs, one co-twin was always intermediately...

Abstract Purpose To investigate whether exposure to systemic antibiotics influences the risk of developing type 2 diabetes and overweight/obesity. Methods The study sample comprised 2209 (110 with incident diabetes) participants from the population-based Cardiovascular Risk in Young Finns Study (YFS) aged 24–39 years in 2001. The exposure was national linked register data on purchased antibiotic courses between 1993 and 2001. Clinical examinations including BMI were conducted in 2001, 2007 and 2011. Participants with prevalent diabetes...

Additional file 3: Figure S2. The birth weight z-scores comparing STOPPAM vs. Intergrowth (IG21) references. The dash line indicates the mean birthweight z-scores.

Schistosoma mansoni is an intestinal parasite with one β-class carbonic anhydrase, SmaBCA. We report the sequence enhancing, production, catalytic activity, and inhibition results of the recombinant SmaBCA. It showed significant catalytic activity on CO2 hydration in vitro with kcat 1.38 × 105 s−1 and kcat/Km 2.33 × 107 M−1 s−1. Several sulphonamide inhibitors, from which many are clinically used, showed submicromolar or nanomolar inhibitory effects on SmaBCA. The most efficient inhibitor with a KI of...

The population in many countries is becoming more diverse. The number of people from foreign backgrounds is growing in Finland as well. The aim of this study was to better understand how the foreign background of a patient affects the dentist’s work. The research was carried out as a semi-structured interview. Six dentists from Helsinki municipality public dental care were interviewed between December 2019 and January 2020. After the interviews were transcribed verbatim, two members...

Supplemental Material for Dementia and Related Comorbidities in the Population Aged 90 and Over in the Vitality 90+ Study, Finland: Patterns and Trends From 2001 to 2018 by Pauliina Halonen, Linda Enroth, Esa Jämsen, Saritha Vargese, and Marja Jylhä in Journal of Aging and Health

ObjectivesTo examine trends in the prevalence of dementia and related comorbidities among the oldest old.MethodsSix repeated cross-sectional surveys were conducted between 2001 and 2018, each including all inhabitants aged over 90 in Tampere, Finland (n = 5386). Co-occurring conditions and their time trends among participants with dementia were examined using logistic regression and generalized estimating equations.ResultsThe prevalence of dementia decreased from 47% in 2007 to 41% in 2018. Throughout the study period, depression was more...

Background Resting heart rate (HR) and its variability (HRV) reflects the cardiac sympathovagal balance that is stimulated by head-up tilting. HRV is influenced by the level of HR, but how much HRV offers additional information about cardiac autonomic tone than HR alone remains unresolved. We examined the relation of resting HR with HRV during head-up tilt. Methods. Hemodynamics of 569 subjects without known cardiovascular diseases and medications with direct cardiovascular effects were recorded using whole-body...

Additional file 5: Figure S4. Two stage individual participant data meta-analysis on the association between malaria in pregnancy (MIP) and small for gestational age (SGA) using the STOPPAM (SGASTOPPAM) vs. the Intergrowth-21 reference (SGAIG21). uOR: unadjusted odds ratio in panels A and B, aOR: adjusted odds ratio in panels C and D controlling for body mass index, gravidity, gestational age at enrolment, HIV, and hemoglobin level at enrolment. In addition, adjusted for gestational age at...

Abstract Background The prevalence of small for gestational age (SGA) may vary depending on the chosen weight-for-gestational-age reference chart. An individual participant data meta-analysis was conducted to assess the implications of using a local reference (STOPPAM) instead of a universal reference (Intergrowth-21) on the association between malaria in pregnancy and SGA. Methods Individual participant data of 6,236 newborns were pooled from seven conveniently identified studies conducted in Tanzania and Malawi from 2003–2018 with data on...