Additional File 2: Supplementary Figure

How much the vaccine contributes to the induction and development of neutralizing antibodies (NAbs) of breakthrough cases relative to those unvaccinated-infected cases is not fully understood. We conducted a prospective cohort study and collected serum samples from 576 individuals who were diagnosed with SARS-CoV-2 Delta strain infection, including 245 breakthrough cases and 331 unvaccinated-infected cases. NAbs were analyzed by live virus microneutralization test and transformation of NAb titer. NAbs titers against SARS-CoV-2 ancestral and Delta...

How much the vaccine contributes to the induction and development of neutralizing antibodies (NAbs) of breakthrough cases relative to those unvaccinated-infected cases is not fully understood. We conducted a prospective cohort study and collected serum samples from 576 individuals who were diagnosed with SARS-CoV-2 Delta strain infection, including 245 breakthrough cases and 331 unvaccinated-infected cases. NAbs were analysed by live virus microneutralization test and transformation of NAb titre. NAbs titres against SARS-CoV-2 ancestral and Delta...

Ecological quality assessment is fundamental to revealing changes in ecological environments before the development of effective ecological conservation policies. The complex ecological environment can be assessed more reliably when multiple remote sensing indices are integrated, such as in the use of the prevalent Remote Sensing-based Ecological Index (RSEI). However, for effective ecological quality assessment, the requirement of acquisition time consistency for images has become an outstanding issue for broadening the application of the RSEI. In...

Additional file 4: Supplementary figure 4. Efficiency of miR-185-3p transfection and functional enrichment analyses of target genes of circ_0002078. (A) Efficiency verification of miR-185-3p overexpression in MEC-1 cells by qRT-PCR. (B) GO analysis results demonstrated that the target genes of circ_0002078 were enriched in positive regulation of gene expression, lymphocyte proliferation, and regulation of B cell apoptotic process. (C) KEGG pathway analysis showed that the target genes were primarily relevant to JAK-STAT signaling pathway and...

Abstract Background Chronic lymphocytic leukemia (CLL) is a heterogeneous B-cell malignancy that lacks specific biomarkers and drug targets. Competing endogenous RNAs (ceRNAs) play vital roles in oncogenesis and tumor progression by sponging microRNAs (miRNAs). Nevertheless, the regulatory mechanisms of survival-related ceRNA networks in CLL remain to be uncovered. Methods We included 865 de novo CLL patients to investigate RNA expression profiles and Illumina sequencing was performed on four CLL patients, two CLL cell lines and...

Additional file 3.

Abstract Pseudomonas aeruginosa infection is a severe acute suppurative ulcer that engulfs virtually the entire tissue in a short period and leads to devastating destruction. Antibiotic therapy is a common approach for the prophylaxis and treatment of P. aeruginosa infection. However, it is often associated with serious side effects, complications, and multidrug resistance. Therefore, it has been a long-standing challenge to explore safe and effective methods for controlling P. aeruginosa infection. Herein, tannin-coordinated nanozyme composite-based...

Additional file 1: Figure S1. SEM of TCNH. Figure S2. EDS of TCN. Figure S3. Optimization of the H2O2 and TCNH concentrations. Figure S4. In vitro antibacterial activity of TCNH against MDR P. aeruginosa (20059, 21173, 21715). Representative photographs of bacterial colonies after intervened by saline, H2O2, TCNH, and TCNH+H2O2. Figure S5. Immunostaining analysis of macrophages and neutrophils in mouse corneas after saline, H2O2, TCNH, and TCNH+H2O2 interventions. Figure S6. In vivo antibacterial activities of...

Abstract Background Ovarian strumal carcinoid is an extremely rare ovarian malignant tumor with limited data on clinical characteristics and survival outcomes. Methods A retrospective study of 119 patients was conducted, including 98 cases identified from literature review, and their clinical characteristics were investigated. The overall survival (OS), disease-specific survival (DSS), recurrence-free survival (RFS), and potential prognostic factors of these patients were also evaluated. Results Lesions of 115 cases were confined to the ovarian while four...

Abstract Background Numerous studies have revealed that long noncoding RNAs (lncRNAs) are closely related to the development of many diseases and carcinogenesis. However, their specific biological function and molecular mechanism in oesophageal squamous cell carcinoma (ESCC) remains unclear. Methods RNA-Seq was performed to determine the differential expressions of lncRNAs in ESCC, and the level of SNHG16 expression was detected in ESCC and intraepithelial neoplasia (IEN) samples. In vitro and in vivo experiments were performed to...

Additional file 1. Supplementary Figure 1. CAV-1 regulated the expression of occludin under hypoxic conditions. (A) bEnd.3 cells were exposed to 1% O2 for 24 h. HIF-1α protein levels were detected by Western blot. (B and C) After transfection with siCAV1 for 48 h, bEnd.3 cells were exposed to 1% O2 for 24 h; Occludin protein levels were detected by Western blot (B) and quantified (C) (*p<0.05 and **p<0.01).

Abstract Acute promyelocytic leukemia (APL) has become curable over 95% patients under a complete chemo-free treatment with all-trans retinoic acid (ATRA) and arsenic trioxide in low-risk patients. Minimizing chemotherapy has proven feasible in high-risk patients. We evaluated oral arsenic and ATRA without chemotherapy as an outpatient consolidation therapy and no maintenance for high-risk APL. We conducted a multicenter, single-arm, phase 2 study with consolidation phases. The consolidation therapy included Realgar–Indigo naturalis formula (60 mg/kg daily...

Abstract Background This study aimed to investigate whether the combination of Macleaya cordata extract (MCE) and Bacillus could improve the laying performance and health of laying hens better. Methods A total of 360 29-week-old Jingbai laying hens were randomly divided into 4 treatments: control group (basal diet), MCE group (basal diet + MCE), Probiotics Bacillus Compound (PBC) group (basal diet + compound Bacillus), MCE + PBC group (basal diet + MCE + compound Bacillus). The...

Additional file 2: Additional Tables 1-5.

Additional file 1. Table S1. Statistical comparison among manual plans and hybrid plans for moderate, hard and easy clinical goal settings. Table S2. Statistical comparison among manual plans and script plans for moderate, hard and easy clinical goal settings. Table S3. Statistical results of planmanual for added goals setting.

Additional file 16: Fig. S3. KEGG analysis of genes within the 3 Mbp IBD-ER.

Additional file 18: Fig. S5. GWAS of fruit firmness. Quantile-Quantile (Q-Q) plots of a genome-wide association analysis for fruit firmness.

Additional file 8: Table S8. The collection sites of 164 pear accessions used in the GWAS in this study.

Additional file 1: Figure S1. Associations between L-carnitine and the risk of gestational diabetes mellitus (GDM) in Chinese women.

Additional file 10: Table S7: Two-way ANOVA test F-value of genes expression levels related to cytokinin biosynthesis and signaling transduction for three C. sinensis cultivars (BY1, HJY and SCZ) affected by development stage (S) and cultivar (C).

Additional file 14: Table S11: Two-way ANOVA test F-value of genes expression levels related to jasmonic biosynthesis and signaling transduction for three C. sinensis cultivars (BY1, HJY and SCZ) affected by development stage (S) and cultivar (C).

Additional file 1: Fig. S1: The ABCDE model and the quartet model of floral organ development in Arabidopsis thaliana.

Additional file 2: Fig. S2: Morphological characteristics of flower organs at three flower developmental stages in three Camellia sinensis varieties. BY1 represents the C. sinensis cv. ‘BaiYe 1’, HJY represents the C. sinensis cv. ‘HuangJinYa’, SCZ represents the C. sinensis cv. ‘SuChaZao’, S1-S3 represent the three flower developmental stages.

Additional file 7: Table S4: Summary of RNA-Seq data and mapping metrics.