Data from: Incubator-independent cell-culture perfusion platform for continuous long-term microelectrode array electrophysiology and time-lapse imagingDirk Saalfrank, Anil Krishna Konduri, Shahrzad Latifi, Rouhollah Habibey, Asiyeh Golabchi, Aurel Vasile Martiniuc, Alois Knoll, Sven Ingebrandt & Axel Blau
Most in vitro electrophysiology studies extract information and draw conclusions from representative, temporally limited snapshot experiments. This approach bears the risk of missing decisive moments that may make a difference in our understanding of physiological events. This feasibility study presents a simple benchtop cell-culture perfusion system adapted to commercial microelectrode arrays (MEAs), multichannel electrophysiology equipment and common inverted microscopy stages for simultaneous and uninterrupted extracellular electrophysiology and time-lapse imaging at ambient CO2 levels. The concept...
To meet the current challenges in human food production, improved understanding of the genetic diversity of crop species that maximizes the selection efficacy in breeding programs is needed. The present study offers new insights into the diversity, genetic structure and demographic history of cultivated rye (Secale cereale L.). We genotyped 620 individuals from 14 global rye populations with a different end use (grain or forage) at 32 genome-wide simple sequence repeat markers. We reveal the...
Data from: Response of mountain Picea abies forests to stand-replacing bark beetle outbreaks: neighbourhood effects lead to self-replacementThorsten Zeppenfeld, Miroslav Svoboda, Robert Justin DeRose, Marco Heurich, Jörg Müller, Pavla Čížková, Martin Starý, Radek Bače & Daniel C. Donato
1. Large, severe disturbances drive many forest ecosystems over the long term, but pose management uncertainties when human experience with them is limited. Recent continent-scale outbreaks of bark beetles across the temperate Northern Hemisphere have raised major concerns as to whether coniferous forests will regenerate back towards pre-outbreak condition and meet possible reforestation objectives. To date, however, analyses of post-outbreak regeneration across broad spatial and temporal scales have been rare, and entirely lacking for many...
To evaluate progress on political biodiversity objectives, biodiversity monitoring provides information on whether intended results are being achieved. Despite scientific proof that monitoring and evaluation increase the (cost) efficiency of policy measures, cost estimates for monitoring schemes are seldom available, hampering their inclusion in policy programme budgets. Empirical data collected from 12 case studies across Europe were used in a power analysis to estimate the number of farms that would need to be sampled per...
Data from: A summary of eight traits of Coleoptera, Hemiptera, Orthoptera and Araneae, occurring in grasslands in GermanyMartin M. Gossner, Nadja K. Simons, Roland Achtziger, Theo Blick, Wolfgang H. O. Dorow, Frank Dziock, Frank Köhler, Wolfgang Rabitsch & Wolfgang W. Weisser
Analyses of species traits have increased our understanding of how environmental drivers such as disturbances affect the composition of arthropod communities and related processes. There are, however, few studies on which traits in the arthropod community are affected by environmental changes and which traits affect ecosystem functioning. The assembly of arthropod traits of several taxa is difficult because of the large number of species, limited availability of trait databases and differences in available traits. We...
Data from: Effects of management on aquatic tree-hole communities in temperate forests are mediated by detritus amount and water chemistryMartin M. Gossner, Peggy Lade, Anja Rohland, Nora Sichardt, Tiemo Kahl, Jürgen Bauhus, Wolfgang W. Weisser & Jana S. Petermann
1. Arthropod communities in water-filled tree-holes may be sensitive to impacts of forest management, for example via changes in environmental conditions such as resource input. 2. We hypothesized that increasing forest management intensity negatively affects arthropod abundance and richness and shifts community composition and trophic structure of tree-hole communities. We predicted that this shift is caused by reduced habitat and resource availability at the forest stand scale as well as reduced tree-hole size, detritus amount...
Data from: Small beetle, large-scale drivers: how regional and landscape factors affect outbreaks of the European spruce bark beetleRupert Seidl, Jörg Müller, Torsten Hothorn, Claus Bässler, Marco Heurich & Markus Kautz
Unprecedented bark beetle outbreaks have been observed for a variety of forest ecosystems recently, and damage is expected to further intensify as a consequence of climate change. In Central Europe, the response of ecosystem management to increasing infestation risk has hitherto focused largely on the stand level, while the contingency of outbreak dynamics on large-scale drivers remains poorly understood. To investigate how factors beyond the local scale contribute to the infestation risk from Ips typographus...
Data from: Genetic differentiation within multiple common grassland plants supports seed transfer zones for ecological restorationWalter Durka, Stefan G. Michalski, Kenneth W. Berendzen, Oliver Bossdorf, Anna Bucharova, Julia-Maria Hermann, Norbert Hölzel & Johannes Kollmann
Ecological restoration of grasslands is increasingly based on regional seeds derived from predefined seed transfer zones. However, the degree and spatial pattern of genetic differentiation among provenances of different seed transfer zones is largely unknown. We assessed the genetic differentiation among eight out of 22 German seed transfer zones for seven common grassland species (Arrhenatherum elatius, Centaurea jacea, Daucus carota, Galium album, Hypochaeris radicata, Knautia arvensis and Lychnis flos-cuculi) using AFLP markers. We analysed genetic...
Technical University Munich8
University of Applied Sciences2
University of Natural Resources and Life Sciences2
Bavarian Forest National Park2
Helmholtz Centre for Environmental Research1
University of Padua1
University of Münster1
Estonian University of Life Sciences1
University of Bologna1
Environment Agency Austria1