A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymeraseHui Wu, Ting Wei, Bingbing Yu, Rui Cheng, Fengtao Huang, Xuelin Lu, Yan Yan, Xionglue Wang, Chenli Liu & Bin Zhu
Transcription termination is one of the least understood processes of gene expression. As the prototype model for transcription studies, the single-subunit T7 RNA polymerase (RNAP) is known to respond to two types of termination signals, but the mechanism underlying such termination, especially the specific elements of the polymerase involved, is still unclear, due to a lack of knowledge with respect to the structure of the termination complex. Here we applied phage-assisted continuous evolution to obtain...
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