Additional file 3: Figure S3. Analysis of blood physiology parameters of control, vehicle- and CurE-treated mice. The following blood physiology functions were assayed: WBC: white blood cell count; RBC: red blood cell count; HGB: hemoglobin; MCV: mean corpuscular volume; RDW: red blood cell volume distribution width; PLT: platelet; LYM: lymphocyte count; MON: monocyte count; NEUT: neutrophil count. The data were shown as mean ± standard deviation. N = 6 for each group, **P < 0.01...

Additional file 5: Figure S5. Overexpression of CDK1 enhanced protein stabilities of UBE2C and AURKA/B. a Expressions of UBE2C and AURKA/B in SW-13_NC and SW-13_CDK1 cells after treatment with CHX (100 μmol/L) at 0, 1, 3, 9, 12 h. b Relative expression of UBE2C, AURKA c and AURKB d in SW-13_NC and SW-13_CDK1 cells after treatment with CHX. Statistical differences were assessed by two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

Additional file 5: Figure S5. Overexpression of CDK1 enhanced protein stabilities of UBE2C and AURKA/B. a Expressions of UBE2C and AURKA/B in SW-13_NC and SW-13_CDK1 cells after treatment with CHX (100 μmol/L) at 0, 1, 3, 9, 12 h. b Relative expression of UBE2C, AURKA c and AURKB d in SW-13_NC and SW-13_CDK1 cells after treatment with CHX. Statistical differences were assessed by two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.

Additional file 6: Figure S6. Silencing the markers of apoptosis or pyroptosis could not rescue CurE-mediated cell death. a Transfection efficacy of knockdown caspase 3 expression in SW-13 cells. b Transfection efficacy of knockdown caspase 3 expression in NCI-H295R cells. c Transfection efficacy of knockdown GSDMD expression in SW-13 cells. b Transfection efficacy of knockdown GSDMD expression in NCI-H295R cells. e Silencing caspase 3 increased necrotic cells triggered by CurE in SW-13 cells. f Silencing...

The simple and effective optical filter is the significantly scientific and technical interest in optical signal processing and communication. Especially, the development of microsystem integration is limited in traditional optical filters, due to the complicated structure, small choice, large cost, etc. In this letter, we report an ultra-narrowband filter based on a metal-cladding resonant waveguide. Therein, the ultra-narrowband resonant mode is achieved based on the resonance screening of incident light and cavity modes. According to...

Supplementary Figures and Tables

Additional file 3. Spatial probability isocontour maps of age group 1, 2, 3 and 5. (a, b, c, and d) Maps depict the distribution of probabilities throughout the harrat based on the locations of visible vents in age group 1, 2, 3 and 5, respectively, without using age-weighting procedure.

Abstract The 180,000 km2 of Arabian lava fields (“harrats” in Arabic) form one of the largest distributed basaltic provinces in the world. The most recent eruption in 1256 AD, on the outskirts of Medina, as well as shallow dike emplacement in 2009, ~ 200 km northeast of the city, suggest future volcanic threat to this area. Harrat Khaybar (~ 1.7 Ma to present) is one of the largest and most compositionally diverse Arabian lava fields;...

Supplemental material, sj-pdf-2-wso-10.1177_17474930221140792 for Human blood metabolites and lacunar stroke: A Mendelian randomization study by Meng-Nan Guo, Xiao-Yan Hao, Jie Tian, Yun-Chao Wang, Jia-Di Li, Yu Fan, Jing-Jing Shi, Dong-Rui Ma, Shuang-Jie Li, Chun-Yan Zuo, Yuan-Yuan Liang, Meng-Jie Li, Si Shen, Fen Liu, Da-Bao Yao, Yu-Ming Xu and Chang-He Shi in International Journal of Stroke

Background:Lacunar stroke accounts for a quarter of all strokes, but little is known about the underlying pathological mechanisms. Analysis of serum metabolites may allow better understanding of the underlying biological processes. Mendelian randomization (MR) can provide information on the causality of associations.Aims:To identify causal relationships between serum metabolites and lacunar stroke.Methods:We applied a two-sample MR analysis to evaluate relationships between 486 serum metabolites and lacunar stroke. The inverse-variance weighted (IVW) method was used to estimate...

Additional file 1. Typical imaging findings of cystic tumours in brain.

Additional file 2. The function of tucidinostat on tumor immunity. a Functional annotation clustering of genes regulated in tumor from CT26 tumor-bearing mice on day 10 post treatment with tucidinostat (25 mg/kg, gavage, daily, n=3) or DMSO as vehicle control (DMSO, n=3). b Representative cytograms (left) or summary histograms (right) for the cell surface PD-L1 expression in CT26 cells following different doses (2.5, 5, 7.5 μM) of vorinostat, tucidinostat, and TMP-195 treatment for 24h. c...

Supplementary results of the manuscript

Fluorescence molecular tomography (FMT) is a novel imaging modality to obtain the three-dimensional (3D) distribution of fluorescence biomarkers. However, the simplified mathematical model and complicated inverse problem limit it to achieving precise results. In this study, the second near-infrared (NIR-II) fluorescence imaging was adopted to mitigate tissue scattering and reduce noise interference. An excitation-based fully connected network was proposed to model the inverse process of NIR-II photon propagation and directly obtain the 3D distribution of...

Nuclear factor I (NFI) family is emerging found playing oncogenic or tumor-suppressive potential in cancers. However, the function and underlying mechanisms of NFIC, in the progression of Lung Squamous Cell Carcinoma (LUSC) remain unclear. Therefore, this study aims to probe into the function of NFIC in the development of LUSC. In the present study, we reported that NFIC was low expressed in human LUSC tissues and cell lines. NFIC inhibited LUSC cell proliferation and promoted...

Additional file 2. The function of tucidinostat on tumor immunity. a Functional annotation clustering of genes regulated in tumor from CT26 tumor-bearing mice on day 10 post treatment with tucidinostat (25 mg/kg, gavage, daily, n=3) or DMSO as vehicle control (DMSO, n=3). b Representative cytograms (left) or summary histograms (right) for the cell surface PD-L1 expression in CT26 cells following different doses (2.5, 5, 7.5 μM) of vorinostat, tucidinostat, and TMP-195 treatment for 24h. c...

Additional file 3. Tucidinostat plus checkpoint blockade induces improved therapeutic efficacy. a Schema of the experiment. For bioluminescence imaging (BLI) in vivo, mouse 4T1-luc (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). Tumors volume...

Additional file 5. Tucidinostat plus checkpoint blockade induces CCL5 secretion. a Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with different doses (12.5, 25, 75 mg/kg, gavage, daily, n=5) of tucidinostat. The expression of CCL5 in peripheral blood serum from CT26 tumor-bearing mice on day 10 post treatment initiation. Mouse CT26 cells (5 ×...

Additional file 1. The effect of tucidinostat on cell proliferation, apoptosis and hepatonephric function. a Comparison of cell proliferation of 4T1, LLC, and CT26 cells treated with different doses (2.5, 5, 7.5 μM) of tucidinostat for 24 h by CCK-8 assay. b Comparison of cell apoptosis of 4T1, LLC, and CT26 cells treated with different doses of tucidinostat (2.5, 5, 7.5 μM) for 6 h by Annexin V-FITC/PI assay. c Mouse CT26 cells (5 ×...

Additional file 3. Spatial probability isocontour maps of age group 1, 2, 3 and 5. (a, b, c, and d) Maps depict the distribution of probabilities throughout the harrat based on the locations of visible vents in age group 1, 2, 3 and 5, respectively, without using age-weighting procedure.

Additional file 5. Spatio-temporal probability isocontour maps obtained by multiplying the values of the spatial analysis of last 300 ka eruptions by their long-term average recurrence rate, 1.1 eruptions/10 kyr. (a, b, and c) Maps show the probabilities of occurrence of at least one eruption in Harrat Khaybar for the next 1, 10, and 1000 years, respectively.

Additional file 1. The performance of the AgNRs-based miRNA analysis using gold macroelectrode accompanies this paper.

Supplemental material, sj-pdf-5-wso-10.1177_17474930221140792 for Human blood metabolites and lacunar stroke: A Mendelian randomization study by Meng-Nan Guo, Xiao-Yan Hao, Jie Tian, Yun-Chao Wang, Jia-Di Li, Yu Fan, Jing-Jing Shi, Dong-Rui Ma, Shuang-Jie Li, Chun-Yan Zuo, Yuan-Yuan Liang, Meng-Jie Li, Si Shen, Fen Liu, Da-Bao Yao, Yu-Ming Xu and Chang-He Shi in International Journal of Stroke

Background:Lacunar stroke accounts for a quarter of all strokes, but little is known about the underlying pathological mechanisms. Analysis of serum metabolites may allow better understanding of the underlying biological processes. Mendelian randomization (MR) can provide information on the causality of associations.Aims:To identify causal relationships between serum metabolites and lacunar stroke.Methods:We applied a two-sample MR analysis to evaluate relationships between 486 serum metabolites and lacunar stroke. The inverse-variance weighted (IVW) method was used to estimate...

Abstract Background Although immune checkpoint inhibitors (ICIs) have influenced the treatment paradigm for multiple solid tumors, increasing evidence suggests that primary and adaptive resistance may limit the long-term efficacy of ICIs. New therapeutic strategies with other drug combinations are hence warranted to enhance the antitumor efficacy of ICIs. As a novel tumor suppressor, histone deacetylase (HDAC) inhibitor tucidinostat has been successfully confirmed to act against hematological malignancies. However, the underlying mechanisms of action for tucidinostat...