Additional file 4: Figure S3. Association of immune checkpoints with Clusters and prognostic risk groups. The expression of immune checkpoints A PD-1, B PD-L1, C CTLA-4, D IDO1, E IDO2, F HAVCR2, and G LAG3 in Clusters 1 and 2. The expressions of H PD-L1, I PD-1, J CTLA-4, K IDO1, L IDO2, M HAVCR2, and N LAG3 in different risk groups. PD-1, programmed cell death 1; PD-L1, programmed cell death ligand 1; CTLA-4, cytotoxic...

Additional file 6: Figure S4. Association of each lncRNA in the identified signature with the immune subtypes. The expression of lncRNA A NSMCE1-DT, B LINC02084, C MYOSLID, D LINC02428, and E MRPS9-AS1 in different immune subtypes. -, not significant, *p < 0.05, and ***p < 0.001.

Additional file 7: Figure S5. Association of each lncRNA in the identified signature with the molecular subtypes. The expression of lncRNA A NSMCE1-DT, B LINC02084, C MYOSLID, D LINC02428, and E MRPS9-AS1 in different molecular subtypes. MSI, microsatellite instability; CIN, chromosomal instability; GS, genome-stable; HM-SNV, hypermutated single nucleotide variants. -, not significant; *p < 0.05, **p < 0.01, and ***p < 0.001.

Abstract Background Triple negative breast cancer (TNBC) is one of the most lethal breast cancer subtypes. Due to a lack of effective therapeutic targets, chemotherapy is still the main medical treatment for TNBC patients. Thus, it is important and necessary to find new therapeutic targets for TNBC. Recent genomic studies implicated the Hippo / Yap signal is over activated in TNBC, manifesting it plays a key role in TNBC carcinogenesis and cancer progression. RBCK1 was...

Additional file 8: Figure S6. Relationship among risk scores and clinical characteristics. Correlations of risk scores and A age, B sex, C T stage, D N stage, E metastatic status, F total stage, G immune score, and H Clusters. ns, not significant; *p < 0.05, **p < 0.01, and ***p < 0.001.

Abstract Background Energy metabolism disorder, especially lipid metabolism disorder, is an important biological characteristic of colon cancer. This research sought to examine the association between lipid metabolism-related long non-coding RNAs (lncRNAs) and prognoses among colon cancer patients. Methods The transcriptome profile and clinical data of patients with colon cancer were retrieved from The Cancer Genome Atlas database. Using consensus clustering, cases were divided into two clusters and Kaplan–Meier analysis was executed to analyze differences in...

Abstract Background Energy metabolism disorder, especially lipid metabolism disorder, is an important biological characteristic of colon cancer. This research sought to examine the association between lipid metabolism-related long non-coding RNAs (lncRNAs) and prognoses among colon cancer patients. Methods The transcriptome profile and clinical data of patients with colon cancer were retrieved from The Cancer Genome Atlas database. Using consensus clustering, cases were divided into two clusters and Kaplan–Meier analysis was executed to analyze differences in...

Additional file 1: Table S1. The clinical characteristics of the patients.

Additional file 10. Detailed region where the representative images of Figure 5A selected from. LC, lesion cord.

Additional file 1. Animal protocol.

Additional file 5. Coding Sequence of Irak1 and Traf6 Genes.

Additional file 9. Detailed region where the representative images of Figure 2A selected from. LC, lesion cord.

Abstract Background Acute spinal cord injury (SCI) is a devastating result of neurological trauma with subsequent microenvironment dyshomeostasis that induces neurotoxic phenotype acquisition by astrocytes, exacerbating neurological function impairment. Exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs) have demonstrated essential therapeutic effects after central nervous system trauma. However, whether hUCMSC-derived exosomes exert therapeutic effects on neurotoxic astrocytes to facilitate SCI function recovery remains unclear. Additionally, the limited efficiency of single exosomes may restrict...

Additional file 1: Table S1. Scoring system for histological changes in the colon. Table S2. The sequences of primers used in this study. Figure S1. ERβ deficiency did not influence the sensorimotor function, memory function, or social interactions in mice following induced experimental colitis. (A) Nest score in the nest building test was performed among the four groups to detect the sensorimotor functions. (B) Spatial memory was assessed by percentage of spontaneous alterations in the...

Abstract Background Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood. Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice. Results In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β...

Abstract Background Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood. Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice. Results In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β...

Additional file 2: Fig. S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 4: Fig. S4. Effects of cilostazol treatment on complete blood counts after CLP. a, b The level of platelets (a), WBC (b), NEUT (c), MONO (d) and LYMPH (e) (n = 5). WBC, white blood cells; NEUT, neutrophil; MONO, monocyte; LYMPH, lymphocyte. The data are presented as the mean ± SEM, *P < 0.05, ***P < 0.001, and ns indicates no significant difference.

Additional file 7: Fig. S7. Effect of LPS and CD40L on TRAFs proteins in EGCs. a Representative western blot images of TRAFs proteins expression in EGCs. b-g Western blot analysis of TRAF1 (b), TRAF2 (c), TRAF3 (d), TRAF4 (e), TRAF5 (f) and TRAF6 (g) expression in different groups of EGCs (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Abstract Background Intestinal barrier dysfunction, which is associated with reactive enteric glia cells (EGCs), is not only a result of early sepsis but also a cause of multiple organ dysfunction syndrome. Inhibition of platelet activation has been proposed as a potential treatment for septic patients because of its efficacy in ameliorating the organ damage and barrier dysfunction. During platelet activation, CD40L is translocated from α granules to the platelet surface, serving as a biomarker of...

Word embedding, a high-dimensional (HD) numerical representation of words generated by machine learning models, has been used for different natural language processing tasks, for example, translation between two languages. Recently, there has been an increasing trend of transforming the HD embeddings into a latent space (e.g. via autoencoders) for further tasks, exploiting various merits the latent representations could bring. To preserve the embeddings’ quality, these works often map the embeddings into an even higher-dimensional latent...

Photochromic sapphires show an optically controllable orange coloration under ambient temperature, which strongly affects the color appearance and market value of gemstone sapphires. An in-situ absorption spectroscopy combined with a tunable excitation light source has been developed to investigate the wavelength- and time dependence of sapphire’s photochromism. An excitation of ≤370 nm and ≥410 nm introduces and removes the orange coloration, respectively, accompanied by a stable absorption band at 470 nm. Both the color enhancement...

Abstract Intestinal ischemia/reperfusion (I/R) causes barrier impairment and bacterial influx. This study explored the protective effects of anisodamine hydrobromide (AH) on intestinal I/R injury caused by cardiopulmonary resuscitation (CPR) after cardiac arrest (CA). After successful CPR, minipigs were randomly divided into two groups (n = 8): saline and AH (4 mg/kg), and then treated with saline or AH via central venous injection, respectively. The same procedures without ventricular fibrillation initiation were conducted in the Sham...

Benefit from their near-unity photoluminescence quantum yield (PL QY), narrow emission band, and widely tunable bandgap, metal halide perovskites have shown promising in light-emitting applications. Despite such promise, how to facile, environmental-friendly, and large-scale prepare solid metal halide perovskite with high emission and stability remains a challenge. Herein, we demonstrate a convenient and environmental-friendly method for the mass synthesis of solid CsPbBr3/Cs4PbBr6 composites using high-power ultrasonication. Adjusting key experimental parameters, bright emitting CsPbBr3/Cs4PbBr6 solids with...

Rapid dispersive liquid-liquid microextraction (DLLME) based on the solidification of a switchable hydrophilicity solvent (SHS) was established for the preconcentration of pyrethroid pesticides from grain prior to determination using high‐performance liquid chromatography (HPLC) with photodiode array (PDA). Lauric acid was selected to be the switchable hydrophilicity solvent due to its conversion between hydrophobic and hydrophilic forms and solidification at low temperature, thereby avoiding a time-consuming centrifugation step. The results show that the optimal conditions for...