Strain NB-1, which can efficiently degrade nitrobenzene, was identified as Pseudomonas frederiksbergensis. NB-1 was resistant to cold and alkali with the widest temperature (4–35 °C) and pH (5–11) adaptive range, compared with other reported nitrobenzene-degrading microorganisms. Based on the Haldane-Andrews model, the real maximum specific growth rate μm', specific affinity aA, and inhibition coefficient Ki were used in response surface methodology (RSM) simultaneously for the first time to guide NB-1 to treat nitrobenzene wastewater. According...

Strain NB-1, which can efficiently degrade nitrobenzene, was identified as Pseudomonas frederiksbergensis. NB-1 was resistant to cold and alkali with the widest temperature (4°C–35°C) and pH (5–11) adaptive range, compared with other reported nitrobenzene-degrading microorganisms. Based on the Haldane-Andrews model, the real maximum specific growth rate μm’, specific affinity aA, and inhibition coefficient Ki were used in response surface methodology (RSM) simultaneously for the first time to guide NB-1 to treat nitrobenzene wastewater. According to...

Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude...

Circular (circ) RNAs are differentially expressed in gastric cancer (GC) and participate in the biological growth of tumor cells. Given that, investigations were performed to unravel the function of circ_0000654 in GC. GC tissue and normal tissue specimens were obtained, in which circ_0000654, microRNA (miR)-149-5p, and inhibin-beta A (INHBA) levels were examined. GC cell line (BGC-823) was transfected to alter circ_0000654 and miR-149-5p expression, thereby observing cell malignancy. Stably-transfected BGC-823 cells were injected into nude...

figures of the result of the rest animals and humans

Additional file 10. Detailed region where the representative images of Figure 5A selected from. LC, lesion cord.

Abstract Background An outbreak of hemorrhagic fever with renal syndrome (HFRS), caused by a Hantavirus, affected nine adult males in the southwest area of Xi’an in November 2020 was analyzed in this study. Methods Clinical and epidemiological data of HFRS patients in this outbreak were retrospectively analyzed. The whole genome of a hantavirus named 201120HV03xa (hv03xa for short) isolated from Apodemus agrarius captured in the construction site was sequenced and analyzed. In addition, nine HFRS...

Additional file 2. Supplementary methods and materials.

Additional file 3. Primers of target genes.

Additional file 3. Primers of target genes.

Additional file 6. Identification of hUCMSC, primary astrocyte and hUCMSC-derived exosomes. A hUCMSCs were identified by the stem cell makers (CD73, CD90 and CD105) and nonstem cell markers (CD45, CD34 and HLA-DR) expression using flow cytometry. B Primary astrocytes were characterized by immunostaining of GFAP, S100β, Vimentin and morphology in bright filed. C The morphology of exosomes detected by transmission electron microscopy. Scale bar = 200 nm. D Diameters and concentrations of exosomes analyzed by...

Additional file 7. A Successful delivery of PKH-26 labeled exosomes to the spinal cord lesion. Scale bar = 50 nm. B The successful delivery of miR-146a-5p to the spinal cord lesion.

Supplementary

Additional file 1. SPIRIT Checklist.

Abstract Objective The purpose of this study is to explore whether electrical impedance tomography (EIT)-guided individualized positive end-expiratory pressure (PEEP) can reduce the incidence of pulmonary complications within 1 week following a craniotomy compared with a single PEEP (PEEP = 6 cmH2O) from dura suturing to extubation. Methods A randomized controlled trial will be conducted at the Second Affiliated Hospital of Soochou University. Five hundred forty patients undergoing a craniotomy in the supine position will...

Additional file 1. Supplementary figures.

Additional file 1: Table S1. Detailed information on the 96 tick libraries examined here. Figure S1. Gene arrangements in tick mitochondrial genomes across eight genera. There are 23 linear maps in 19 species of Haemaphysalis, nine in Amblyomma species, two in Bothriocroton species, three in two Hyalomma species, 13 in 10 Rhipicephalus species, 18 in 16 Ixodes species, one of Nuttalliellidae species and 26 linear maps of Argasid ticks. Each mitochondrial genome has 13 protein-coding...

Additional file 11. Methods.

Additional file 12. Supplementary discussion.

Additional file 13. Supplementary results.

Additional file 2. Table S2: Adjuvant treatment information.

Additional file 3. Table S3: Performance of three strategies.

Additional file 7. Figure S4: ctDNA statuses and disease-related events of patients during follow-up periods. Swimmer plot illustrating the ctDNA statuses, adjuvant therapies, and pathological events of all patients. Abbreviations: LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma, AT – adjuvant therapy.

Additional file 8. Figure S5: Prognostic values of ctDNA detection based on clonal and subclonal mutations. A). The recurrence-free survival analysis of patients stratified by ctDNA detection based on only clonal mutation profiles. B). The recurrence-free survival analysis of patients stratified by ctDNA detection based on all clonal and subclonal mutations.

Additional file 9. Figure S6: ctDNA testing, LDCT scans, and disease-related events of patients during follow-up periods. . Swimmer plot illustrating the first positive ctDNA testing, the last negative LDCT scans, and pathological events of patients that experienced recurrence or deceased. B). The original and adjusted time intervals between the first positive ctDNA testing and final LDCT scans that detected disease recurrence. Abbreviations: LDCT – low-dose computed tomography, LUAD – lung adenocarcinoma, LUSC - lung...