Objectives. Urinary albumin excretion is a risk marker for cardiovascular disease (CVD). Studies suggest that urinary orosomucoid may be a more sensitive marker of general endothelial dysfunction than albuminuria. The aim of this population-based cross-sectional study was to examine the associations between urinary orosomucoid to creatinine ratio (UOCR), urinary albumin to creatinine ratio (UACR) and subclinical CVD. Design. From the Tromsø Study (2007/2008), we included all men and women who had measurements of urinary orosomucoid...

Additional file 1.

Abstract Background The long-term outcomes of Ulcerative colitis (UC) after discontinuation of biological therapy are largely unknown. There is also a lack of accurate and validated markers that can predict outcome after withdrawal accurately. The aims of this study were to describe the long-term outcomes in UC patients following cessation of anti-TNF therapy and explore potential biomarkers as an approach towards precision medicine. Methods Seventy-five patients with moderate to severe UC treated to remission with...

Abstract Background Based on findings of increasing alcohol consumption in older adults, it is important to clarify the health consequences. Using data from the Tromsø study, we aimed to investigate the relationship between different levels of alcohol consumption in old adulthood and self-rated health trajectories and all-cause mortality. Methods This is an epidemiological study utilizing repeated measures from the Tromsø study cohort. It allows follow-up of participants from 1994 to 2020. A total of 24,590...

Additional file 1. Online Appendix.

Additional file 1. Supplementary Figure 1. Analysis of primary ECs by flow cytometry following one-time or double transfection with miRNA inhibitors. Supplementary Figure 2. Analysis of monocyte purity by flow cytometry following enrichment from PBMCs using negative selection. Supplementary Figure 3. Analysis of primary ECs by light microscopy and flow cytometry following transfection with the respective miRNA mimics. Supplementary Figure 4. Analysis of monocytes by flow cytometry following transfection with the respective miRNA mimics.

Abstract Background A comprehensive dissection of the role of microRNAs (miRNAs) in gene regulation and subsequent cell functions requires a specific and efficient knockdown or overexpression of the miRNA of interest; these are achieved by transfecting the cell of interest with a miRNA inhibitor or a miRNA mimic, respectively. Inhibitors and mimics of miRNAs with a unique chemistry and/or structural modifications are available commercially and require different transfection conditions. Here, we aimed to investigate how...

Abstract Background Injuries are one of the leading causes of death worldwide. Bystanders at the scene can perform first aid measures before the arrival of health services. The quality of first aid measures likely affects patient outcome. However, scientific evidence on its effect on patient outcome is limited. To properly assess bystander first aid quality, measure effect, and facilitate improvement, validated assessment tools are needed. The purpose of this study was to develop and validate...

Abstract Background Differences in the sociodemographic characteristics of participants and non-participants in population-based studies may introduce bias and reduce the generalizability of research findings. This study aimed to compare the sociodemographic characteristics of participants and non-participants of the seventh survey of the Tromsø Study (Tromsø7, 2015–16), a population-based health survey. Methods A total of 32,591 individuals were invited to Tromsø7. We compared the sociodemographic characteristics of participants and non-participants by linking the Tromsø7 invitation file...

To explore whether plaque and gingival bleeding are more frequently experienced by adolescents with juvenile idiopathic arthritis (JIA) compared to matched controls without JIA; explore whether surface- and site-specific periodontal outcomes vary between the two groups; and for participants with JIA, investigate associations between disease-specific features and periodontal outcomes. In this comparative cross-sectional study, selected surfaces, and sites of index teeth in 10–16-year-olds with JIA and matched controls were examined by modified versions of Simplified...

To explore whether plaque and gingival bleeding are more frequently experienced by adolescents with juvenile idiopathic arthritis (JIA) compared to matched controls without JIA; explore whether surface- and site-specific periodontal outcomes vary between the two groups; and for participants with JIA, investigate associations between disease-specific features and periodontal outcomes. In this comparative cross-sectional study, selected surfaces, and sites of index teeth in 10–16-year-olds with JIA and matched controls were examined by modified versions of Simplified...

Additional file 1: Table S1. Characteristics of the participants ≥60 years according to survey. The Tromsø Study 1994–2016. aOnly participants < 70 years were asked the question “how often do you drink 6+ units in one occasion” in 1994–95. bThe proportion includes the use of either or both sleeping pills/tranquilisers. In 1994–95, the time frame asked was “during the last two weeks”, while in the three subsequent surveys it was “during the last four weeks”....

Additional file 1. Supplementary Figure 1. Analysis of primary ECs by flow cytometry following one-time or double transfection with miRNA inhibitors. Supplementary Figure 2. Analysis of monocyte purity by flow cytometry following enrichment from PBMCs using negative selection. Supplementary Figure 3. Analysis of primary ECs by light microscopy and flow cytometry following transfection with the respective miRNA mimics. Supplementary Figure 4. Analysis of monocytes by flow cytometry following transfection with the respective miRNA mimics.

Abstract Background Injuries are one of the leading causes of death worldwide. Bystanders at the scene can perform first aid measures before the arrival of health services. The quality of first aid measures likely affects patient outcome. However, scientific evidence on its effect on patient outcome is limited. To properly assess bystander first aid quality, measure effect, and facilitate improvement, validated assessment tools are needed. The purpose of this study was to develop and validate...

Abstrac Background and objectives Acetabular dysplasia in young adults occurs, despite screening for developmental hip dysplasia (DDH) in the neonatal period. We aimed to examine how early life factors predict radiographic measurements of acetabular dysplasia at 18–19 years of age. Methods From a previous randomized trial (n = 12,014; 1988–90) evaluating the role of hip ultrasound in newborn screening of DDH, 4469 participants (2193 males) were invited to a follow-up 18 years later (2007–09), of...

Additional file 1:Supplementary Table 1. Primer sequences used in site-directed mutagenesis. Supplementary Figure 1. Protein expression levels of BRCA1 variants determined by western blot analysis. (A) The figure shows images from one representative replicate for the fusion protein DBD-BRCT. HEK293FT cells were transiently transfected with DBD-BRCT WT, known benign (green) and pathogenic (red) control variants, and 11 missense BRCA1 VUSs. Cells were harvested 48 h post transfection, and 7 µg total protein retrieved from cell...

Abstract Background Damaging alterations in the BRCA1 gene have been extensively described as one of the main causes of hereditary breast and ovarian cancer (HBOC). BRCA1 alterations can lead to impaired homologous recombination repair (HRR) of double-stranded DNA breaks, a process which involves the RING, BRCT and coiled-coil domains of the BRCA1 protein. In addition, the BRCA1 protein is involved in transcriptional activation (TA) of several genes through its C-terminal BRCT domain. Methods In this...

Additional file 5. Results from follow-up measurements for the unmatched cohort.

Additional file 1: Fig. S1. Boxplot of IL1RL cytokine measurements comparing HC, LTR and patients in remission with relapse. CT FC= Cycle threshold fold change, HC= Healthy controls, LTR=Long-term remission.

Abstract Background Although the life expectancy of people living with HIV has increased, they are still often disconnected from society through stigma and discrimination. Peer support has been found to increase social support. Given the limited research on peer support from the providers’ perspective, this study explored how peer supporters experience their roles and contributions in outpatient clinics (OPCs). Additionally, healthcare professionals’ perceptions of working with peer supporters in OPCs were examined. Methods This qualitative...

Abstract Background Suboptimal medication use contributes to a substantial proportion of hospitalizations and emergency department visits in older adults. We designed a clinical pharmacist intervention to optimize medication therapy in older hospitalized patients. Based on the integrated medicine management (IMM) model, the 5-step IMMENSE intervention comprise medication reconciliation, medication review, reconciled medication list upon discharge, patient counselling, and post discharge communication with primary care. The objective of this study was to evaluate the effects of...

The relationship between platelet count and risk of major bleeding in patients with venous thromboembolism (VTE) during anticoagulation remains unclear. We therefore investigated the association between platelet count, measured at VTE diagnosis and before the thrombotic event, and risk of major bleeding. Participants comprised 744 patients with incident VTE derived from the Tromsø Study. Major bleedings were recorded during the first year after VTE. Cox-regression was used to calculate hazard ratios (HRs) for major bleeding...

Additional file 1: Supplementary Table 1. Odds ratios for participation by sex, Tromsø7 (2015-2016).

Additional file 1: Table S1. Characteristics of the participants ≥60 years according to survey. The Tromsø Study 1994–2016. aOnly participants < 70 years were asked the question “how often do you drink 6+ units in one occasion” in 1994–95. bThe proportion includes the use of either or both sleeping pills/tranquilisers. In 1994–95, the time frame asked was “during the last two weeks”, while in the three subsequent surveys it was “during the last four weeks”....

Abstract Background Based on findings of increasing alcohol consumption in older adults, it is important to clarify the health consequences. Using data from the Tromsø study, we aimed to investigate the relationship between different levels of alcohol consumption in old adulthood and self-rated health trajectories and all-cause mortality. Methods This is an epidemiological study utilizing repeated measures from the Tromsø study cohort. It allows follow-up of participants from 1994 to 2020. A total of 24,590...