ObjectiveThis systematic review and meta-analysis aimed to evaluate the effect of respiratory muscle training on respiratory muscle strength, lung function, cardiopulmonary fitness, and quality of life for chronic kidney disease patients.MethodsPubMed, Embase, Web of Science Core Collection, and Cochrane Central Register of Controlled Trials published randomized controlled trials that evaluated the effect of respiratory muscle training for chronic kidney disease patients from inception to December 2021, and rerun on September 2022. The quality of included...

Additional file 10. Figure S7: Prognostic value of presurgical ctDNA detection. A). The recurrence-free survival analysis of patients stratified by presurgical ctDNA detection. B). The multi-variant Cox regression for presurgical ctDNA detection. Abbreviations: RFS – recurrence-free survival, LNM – lymph node metastasis, LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma.

Additional file 11. Methods.

Additional file 1. Table S1: Patient demography.

Additional file 4. Figure S1: Availability of plasma samples. The availability of plasma samples for analysis at each schedule collection time point. Blue and red blocks denote samples collected before and after disease recurrence, respectively. Abbreviations: LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma, RFS – recurrence-free survival, AT – adjuvant therapy, RT – radiotherapy, LNM – lymph node metastasis.

Additional file 7. Figure S4: ctDNA statuses and disease-related events of patients during follow-up periods. Swimmer plot illustrating the ctDNA statuses, adjuvant therapies, and pathological events of all patients. Abbreviations: LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma, AT – adjuvant therapy.

Additional file 8. Figure S5: Prognostic values of ctDNA detection based on clonal and subclonal mutations. A). The recurrence-free survival analysis of patients stratified by ctDNA detection based on only clonal mutation profiles. B). The recurrence-free survival analysis of patients stratified by ctDNA detection based on all clonal and subclonal mutations.

Additional file 10: Figure S1. A. Thedifferentially expressed circRNAs between the high metastasis NPC cell line S18 and the low metastasis NPC cell line S26 (GSE137543, the RPM value ≥ 2and Fold changes ≥ 1.5). Circular RNA circPVT1 is highly expressed in NPC cell line S18. B. The Venn diagram of data sets GSE137543 and PRJNA391554. Circular RNA circPVT1 was identified for its high abundance in both sets of data.C. circPVT1 is mainly localized in...

Additional file 14: Figure S5. c-Myc is ubiquitinated substrate of β-TrCP. A. The expression of β-TrCP was examined in CNE2 and 5-8F cells after overexpression or knockdown of circPVT1 using western blotting.B. The β-TrCP binding proteins were identified in CNE2 cells by immunoprecipitation followed by LC-MS/MS.C. The interaction of c-Myc and β-TrCP was predicted by Molecular Docking ( http://hdock.phys.hust.edu.cn/ ). The blue part is the WD40 repeat domain of β-TrCP.D. The expression of c-Myc was...

Additional file 15: Figure S6. circPVT1 promotes the migration and invasion of NPC cells by regulating cell adhesion andcytoskeleton remodeling. The 231 differentially expressed proteins regulated by c-Myc from LC-MS/MS data using the Ingenuity Pathway Analysis (IPA) software.

Additional file 1: Supplemental Table 1. Clinicopathological data of 60 NPC and 26 NPE tissues measured by qRT-PCR.

Additional file 2: Supplemental Table 2. Clinicopathological data of 159 paraffin-embedded NPC tissues and 29 non-neoplastic nasopharyngeal epithelialtissues for in situhybridization.

Additional file 4: Supplemental Table 4. List of antibodies for immunohistochemistry, western blotting, immunofluorescence, and RNA pull-down experiments.

Additional file 6: Supplemental Table 6. The potential β-TrCP-interacting proteins in CNE2 cells identified by LC-MS/MS spectrometry after pull-down with β-TrCP antibody.

Abstract Background Circular RNAs (circRNAs) act as gene expression regulators and are involved in cancer progression. However, their functions have not been sufficiently investigated in nasopharyngeal carcinoma (NPC). Methods The expression profiles of circRNAs in NPC cells within different metastatic potential were reanalyzed. Quantitative reverse transcription PCR and in situ hybridization were used to detect the expression level of circPVT1 in NPC cells and tissue samples. The association of expression level of circPVT1 with clinical...

Supplemental Material for Leukocyte and Platelet Related Inflammatory Indicators and Risk of Carotid and Femoral Plaques: A Population-Based Cross-Sectional Study in Southeast China by Ruimei Feng, PhD, Yiquan Dai, MM, Shanshan Du, PhD, Wenbin Liang, PhD, Hongyu Chen, MM, Cheng Chen, MM, Tianmin He, MM, Tao Tao, PhD, Zhijian Hu, PhD, Pingfan Guo, MD, and Weimin Ye, PhD, MD in Angiology

The processes leading to the N2+ lasing are rather complex and even the population distribution after the pump laser excitation is unknown. In this paper, we study the population distribution at electronic and vibrational levels in N2+ driven by ultra-short laser pulse at the wavelengths of 800 nm and 400 nm by using the quantum-mechanical time-domain incoherent superposition model based on the time-dependent Schrodinger equation and the quasi-classical model assuming instantaneous ionization injection described by...

Additional file 2. Analysis of differential miRNA in chickens infected with Newcastle disease virus (NDV) or avian influenza virus (AIV). (A) The Vene Map different miRNAs of chicken infection NDV or AIV. (B) The Heat Map differential miRNAs of chicken infection with NDV. (C) The Volcano Map different miRNAs of chicken infection with AIV. The miRNA microarray using the R package “limma”. |logFC|> 2 and a p-value < 0.05 were set as the threshold to...

Abstract Innate immunity plays an essential role in preventing the invasion of pathogenic microorganisms. However, innate immunity is a double-edged sword, whose excessive activation is detrimental to immune homeostasis and even leads to a “cytokine storm” of the infected host. The host develops a series of negative regulatory mechanisms to balance the immune response. Here, we report a negative regulatory mechanism of chicken innate immunity mediated by miRNA. In the GEO database, we found that...

Abstract Background Birth weight is considered not only to undermine future growth, but also to induce lifelong diseases; the aim of this study is to explore the relationship between birth weight and adult bone mass. Methods We performed multivariable regression analyses to assess the association of birth weight with bone parameters measured by dual-energy X-ray absorptiometry (DXA) and by quantitative ultrasound (QUS), independently. We also implemented a systemic Mendelian randomization (MR) analysis to explore the...

The thermal-rheological structure of the continental lithosphere is an important indicator of geodynamic processes. Tectonic compression and extension may change thermal-rheological structure of lithosphere. However, the thermal-rheological evolution over geological time may be difficult to determine because of overprints of subsequent geological events. Mantle xenolith temperature and pressure conditions can differentiate the lithospheric thermal structure over geological time and indicate the lithospheric rheological strength. Chronological and temperature-pressure condition data from 11 mantle xenoliths in Cenozoic...

Abstract Since its discovery in the 1990s, the DNA vaccine has been of great interest because of its ability to elicit both humoral and cellular immune responses while showing relative advantages regarding producibility, stability and storage. However, when applied to human subjects, inadequate immunogenicity remains as the greatest challenge for the practical use of DNA vaccines. In this study, we generated a DNA vaccine Δ42PD1-P24 encoding a fusion protein comprised of the HIV-1 Gag p24...

Abstract Background Previous studies on associations of alcohol use with memory decline showed inconclusive results. We examined these associations using longitudinal data from the Guangzhou Biobank Cohort Study (GBCS) and explored whether these associations varied by sex and age group. Methods Memory function was assessed by delayed 10-word recall test (DWRT) and immediate 10-word recall test (IWRT) at both baseline (2003–2008) and follow-up (2008–2012) examinations, expressed as the mean annual change and mean annual rate...

Supplementary Material 1. Changes in the DWRT and IWRT scores and memory cognitive impairment during an average follow-up of 4.1 years by baseline alcohol use and age group

Supplementary Material 1. Changes in the DWRT and IWRT scores and memory cognitive impairment during an average follow-up of 4.1 years by baseline alcohol use and age group