2 Works

Seasonal dynamics of sinking organic matter in the Pacific Arctic Ocean revealed by nitrogen isotope ratios of amino acids

Hyuntae Choi, Eun Jin Yang, Sung-Ho Kang, Dongseon Kim & Kyung-Hoon Shin
Biological sources of organic matter in sinking particles were investigated using nitrogen isotope analysis of amino acids in the Pacific Arctic Ocean, which has experienced a rapidly changing climate, sea-ice retreat, and enhanced primary production over the past few decades. The average trophic position based on differences in the nitrogen isotope ratios of glutamic acid and phenylalanine indicates the relative proportions of photoautotrophs and heterotrophs in sinking particles. Decreasing values (close to 1.0) in summer...

DNA metabarcoding marker choice skews perception of marine eukaryotic biodiversity

Jordan M Casey, Emma Ransome, Allen G Collins, Angka Mahardini, Eka M Kurniasih, Andrianus Sembiring, Nina M D Schiettekatte, Ni Kadek Dita Cahyani, Aji Wahyu Anggoro, Mikaela Moore, Abby Uehling, Mahdi Belcaid, Paul H Barber, Jonathan B Geller & Christopher P Meyer
DNA metabarcoding is an increasingly popular technique to investigate biodiversity; however, many methodological unknowns remain, especially concerning the biases resulting from marker choice. Regions of the cytochrome c oxidase subunit I (COI) and 18S rDNA (18S) genes are commonly employed “universal” markers for eukaryotes, but the extent of taxonomic biases introduced by these markers and how such biases may impact metabarcoding performance is not well quantified. Here, focusing on macro-eukaryotes, we use standardized sampling from...

Registration Year

  • 2021
    2

Resource Types

  • Dataset
    2

Affiliations

  • Korea Institute of Ocean Science and Technology
    2
  • The University of Texas at Austin
    1
  • Korea Polar Research Institute
    1
  • PSL Research University
    1
  • Moss Landing Marine Laboratories
    1
  • Smithsonian Institution
    1
  • University of Hawaii at Manoa
    1
  • Queen's University
    1
  • Imperial College London
    1
  • Hanyang University
    1