Gold nanoparticles have a high potential to be a treatment of diseases by their specific drug delivery properties and multivalent receptor stimulation. For the present project, spherical gold nanoparticles were synthesised and functionalised with the muscarinic receptor antagonist atropine (Au-MUDA-AT NPs). The diameter of the gold core could precisely be controlled by using different synthetic methods and reducing agents resulting in functionalised gold nanoparticles with diameters ranging from 8 to 16 nm. The ability to...

Complex socio-economic, political and demographic factors have driven the increased conversion of Europe’s semi-natural grasslands to intensive pastures. This trend is particularly strong in some of the most biodiverse regions of the continent, such as Central and Eastern Europe. Intensive grazing is known to decrease species diversity and alter the composition of plant and insect communities. Comparatively little is known, however, about how intensive grazing influences plant functional traits related to pollination and the structure...

Fixation behavior toward persons in static scenes varies considerably between individuals. However, it is unclear whether these differences generalize to dynamic stimuli. Here, we examined individual differences in the distribution of gaze across seven person features (i.e. body and face parts) in static and dynamic scenes. Forty-four participants freely viewed 700 complex static scenes followed by eight director-cut videos (28,925 frames). We determined the presence of person features using hand-delineated pixel masks (images) and Deep...

Fixation behavior toward persons in static scenes varies considerably between individuals. However, it is unclear whether these differences generalize to dynamic stimuli. Here, we examined individual differences in the distribution of gaze across seven person features (i.e. body and face parts) in static and dynamic scenes. Forty-four participants freely viewed 700 complex static scenes followed by eight director-cut videos (28,925 frames). We determined the presence of person features using hand-delineated pixel masks (images) and Deep...

Additional file 1: Text S1. Metabolic versatility in Pantoea agglomerans DAPP-PG 734. Table S1. Antibiotic resistance gene profile in the genome of Pantoea agglomerans DAPP-PG 734. Figure S1. Genomic islands in Pantoea agglomerans DAPP-PG 734. Figure S2. MAUVE alignment of Pantoea agglomerans DAPP-PG 734 plasmid 1 and Pantoea vagans C9-1 pPag3. Figure S3. MAUVE alignmentof Pantoea agglomerans DAPP-PG 734 plasmid 3 and Pantoea vagans C9-1 pPag1. Figure S4. Gene cluster for degradation of fructoselysine in...

Additional file 1: Text S1. Metabolic versatility in Pantoea agglomerans DAPP-PG 734. Table S1. Antibiotic resistance gene profile in the genome of Pantoea agglomerans DAPP-PG 734. Figure S1. Genomic islands in Pantoea agglomerans DAPP-PG 734. Figure S2. MAUVE alignment of Pantoea agglomerans DAPP-PG 734 plasmid 1 and Pantoea vagans C9-1 pPag3. Figure S3. MAUVE alignmentof Pantoea agglomerans DAPP-PG 734 plasmid 3 and Pantoea vagans C9-1 pPag1. Figure S4. Gene cluster for degradation of fructoselysine in...

Abstract Background The Hypotension Prediction Index (HPI) displays an innovative monitoring tool which predicts intraoperative hypotension before its onset. Case presentation We report the case of an 84-year-old Caucasian woman undergoing major spinal surgery with no possibility for the transfer of blood products given her status as a Jehovah’s Witness. The hemodynamic treatment algorithm we employed was based on HPI and resulted in a high degree of hemodynamic stability during the surgical procedure. Further, the...

Introduction: Urinary tract infections (UTI) are a leading cause of bacterial infections in women. Despite acute treatment, 30–50% of women who have a UTI will experience a recurrence within 6–12 months. In this review, the focus will be on the personal psychosocial impacts of recurrent UTI. Methods: A PubMed/MEDLINE literature search was carried out from 2000 to 2020 in order to identify any recent high-quality meta-analyses or systematic reviews on these topics. Results: One systematic...

Miscanthus, a C4 perennial rhizomatous grass, is capable of growing in varied climates and soil types in Europe, including on marginal lands. It can produce high yields with low nutrient inputs when harvested after complete senescence. Senescence induction and rate depend on complex genetic, environmental, and management interactions. To explore these interactions, we analysed four miscanthus hybrids (two novel seed-based hybrids, GRC 3 (M. sinensis × sinensis) and GRC 14 (M. sacchariflorus × sinensis); GRC...

Additional file 4: Primers used for sgRNA off-target analysis.

Additional file 2: Table S1. Genome-wide eQTLs identified at 20 DAF. Table S2. Genome-wide eQTLs identified at 40 DAF. Table S3. Genome-wide eGene-eQTLs identified at 20 DAF. Table S4. Genome-wide eGene-eQTLs identified at 40 DAF. Table S5. Results of GO enrichment analysis of specifically identified eGenes at 20 DAF. Table S6. Results of GO enrichment analysis of specifically identified eGenes at 40 DAF. Table S7. Summary of ATAC-Seq data. Table S8. List of homoeologous genes...

Supplemental material, sj-docx-1-ipe-10.1177_20416695221128844 for Individual fixation tendencies in person viewing generalize from images to videos by Maximilian D. Broda and Benjamin de Haas in i-Perception

Additional file 1: This file contains compared networks of children with silence in specific situation and children without silence in specific situations, node predicatability of total sample, strength comparison between nodes of total sample and strength comparison between nodes of subsample.

Abstract Background Molar Incisor Hypomineralisation (MIH) remains a challenge for clinicians underlining the gap in the literature regarding the condition. The study aimed to record knowledge and attitudes of Greek dentists regarding diagnosis and treatment of MIH and correlate findings with non-dental characteristics. Methods It is a cross-sectional study based on a questionnaire consisting of 37 multiple-choice questions. Data regarding diagnosis, aetiopathogenesis, and clinical management of MIH were collected from active members of three Greek...

To provide the most comprehensive picture of species phylogeny and phylogeography of European roe deer (Capreolus capreolus), we analysed mtDNA control region (610 bp) of 1469 samples of roe deer from Central and Eastern Europe and included in the analyses an additional 1,541 mtDNA sequences from GenBank from other regions of the continent. We detected two mtDNA lineages of the species: European and Siberian (an introgression of C. pygargus mtDNA into C. capreolus). The Siberian...

Plant phenology, i. e. the timing of life cycle events, is related to individual fitness and species distribution ranges. Among the environmental factors, phenology is mostly driven by temperature and day length. Rapid adaptation of their phenology may also be important for the success of invasive plant species. Our main aim was to understand how the performance, timing, and temperature dependence of the phenology of the invasive legume Lupinus polyphyllus varies with latitude. L. polyphyllus...

Additional file 2: Mutation spectra of SCLC cell lines. Cell lines were established from AV-PR.Cre (n=3) and AV-PRL.Cre (n=5) SCLC tumors and analyzed by deep sequencing of the sgRNA target regions in Trp53, Rb1 and Rbl2. Depicted is the frequency of wild-type and mutant reads. For mutant reads, the Top5 mutations are color-coded as deletions or insertions and labelled with the number of deleted, inserted or substituted base pairs. Less frequent mutations are summarized as...

Abstract Background In vivo gene editing of somatic cells with CRISPR nucleases has facilitated the generation of autochthonous mouse tumors, which are initiated by genetic alterations relevant to the human disease and progress along a natural timeline as in patients. However, the long and variable, orthotopic tumor growth in inner organs requires sophisticated, time-consuming and resource-intensive imaging for longitudinal disease monitoring and impedes the use of autochthonous tumor models for preclinical studies. Methods To facilitate...

Abstract Background In vivo gene editing of somatic cells with CRISPR nucleases has facilitated the generation of autochthonous mouse tumors, which are initiated by genetic alterations relevant to the human disease and progress along a natural timeline as in patients. However, the long and variable, orthotopic tumor growth in inner organs requires sophisticated, time-consuming and resource-intensive imaging for longitudinal disease monitoring and impedes the use of autochthonous tumor models for preclinical studies. Methods To facilitate...

Abstract Pantoea agglomerans DAPP-PG 734 was isolated as endophyte from knots (tumors) caused by Pseudomonas savastanoi pv. savastanoi DAPP-PG 722 in olive trees. To understand the plant pathogen-endophyte interaction on a genomic level, the whole genome of P. agglomerans DAPP-PG 734 was sequenced and annotated. The complete genome had a total size of 5′396′424 bp, containing one circular chromosome and four large circular plasmids. The aim of this study was to identify genomic features that...

Additional file 1: Fig. S1. Overview of experimental and research analysis methods. Fig. S2. Venn diagram of the distribution of genes regulated by different types of eQTLs (local eQTL and distant eQTL) at 20 DAF and 40 DAF. (a) Distribution number of genes which were regulated by different types of eQTLs at 20 DAF. (b) Distribution number of genes which were regulated by different types of eQTLs at 40 DAF. Fig. S3. Manhattan plot of...

Additional file 3. Review history.

Host plant specialization across herbivorous insects varies dramatically, but while the molecular mechanisms of host-plant adaptations are increasingly known, we often lack a comprehensive understanding of the selective forces that favor specialization. The milkweed bugs (Heteroptera: Lygaeinae) are engaged in ancestrally specialized associations with plants of the Apocynaceae from which they commonly sequester cardiac glycosides for defense, facilitated by resistant Na+/K+-ATPases and adaptations for transport, storage and discharge of toxins. Here, we show that three...

Additional file 2. Studies on feed with added flavors (intervention) as compared to unflavored feed (control) in animal experiments.

Additional file 1: Table S1. Patient´s consent on the application of different blood products.