Additional file 1: Fig.S1. A PCR amplification of α-L-rhamnosidase gene from 11 positive clones (N1, N4, N5, N6, N8, N10, N12, N13,N14, N24 and N28). B SDS-PAGE analysis of N-glycosidase F treated N12-Rha.Lane 1, untreated N12-Rha; lane 2, N12-Rha treated with N-Glycosidase F. Fig.S2. Ramachandran plot A and Verify-3D B of N12-Rha model

Additional file 1: Fig.S1. A PCR amplification of α-L-rhamnosidase gene from 11 positive clones (N1, N4, N5, N6, N8, N10, N12, N13,N14, N24 and N28). B SDS-PAGE analysis of N-glycosidase F treated N12-Rha.Lane 1, untreated N12-Rha; lane 2, N12-Rha treated with N-Glycosidase F. Fig.S2. Ramachandran plot A and Verify-3D B of N12-Rha model

Abstract αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Aspergillus niger JMU-TS528 had significantly higher catalytic activity on α-1,6 glycosidic bond than α-1,2 glycosidic bond, and had no activity on α-1,3 glycosidic bond. The activities of hydrolyzed hesperidin and naringin were 7240 U/mL and 945 U/mL, respectively, which are 10.63 times that of native α-L-rhamnosidase. The activity could maintain more than 80% at pH 3–6...

Abstract Background Job loss after a cancer diagnosis can lead to long-term financial toxicity and its attendant adverse clinical consequences, including decreased treatment adherence. Among women undergoing (neo)adjuvant chemotherapy for breast cancer, access to work accommodations (e.g., sick leave) is associated with higher job retention after treatment completion. However, low-income and/or minority women are less likely to have access to work accommodations and, therefore, are at higher risk of job loss. Given the time and...

Abstract αL-rhamnosidase (EC 3.2.1.40) has been widely used in food processing and pharmaceutical preparation. The recombinant α-L-rhamnosidase N12-Rha from Aspergillus niger JMU-TS528 had significantly higher catalytic activity on α-1,6 glycosidic bond than α-1,2 glycosidic bond, and had no activity on α-1,3 glycosidic bond. The activities of hydrolyzed hesperidin and naringin were 7240 U/mL and 945 U/mL, respectively, which are 10.63 times that of native α-L-rhamnosidase. The activity could maintain more than 80% at pH 3–6...

Background/aims The stochastic arrival of patients at hospital emergency departments complicates their management. More than 50% of a hospital's emergency department tends to operate beyond its normal capacity and eventually fails to deliver high-quality care. To address this concern, much research has been carried out using yearly, monthly and weekly time-series forecasting. This article discusses the use of hourly time-series forecasting to help improve emergency department management by predicting the arrival of future patients. Methods...

Additional file 1. SPIRIT Checklist for Trials.

Additional file 1. SPIRIT Checklist for Trials.

Abstract Background Job loss after a cancer diagnosis can lead to long-term financial toxicity and its attendant adverse clinical consequences, including decreased treatment adherence. Among women undergoing (neo)adjuvant chemotherapy for breast cancer, access to work accommodations (e.g., sick leave) is associated with higher job retention after treatment completion. However, low-income and/or minority women are less likely to have access to work accommodations and, therefore, are at higher risk of job loss. Given the time and...