24 Works

Using vertebrate environmental DNA from seawater in biomonitoring of marine habitats

Eva Egelyng Sigsgaard, Felipe Torquato, Tobias Guldberg Frøslev, Alec B. M. Moore, Johan Mølgård Sørensen, Pedro Range, Radhouane Ben Hamadou, Steffen Sanvig Bach, Peter Rask Møller & Philip Francis Thomsen
Conservation and management of marine biodiversity depends on biomonitoring of marine habitats, but current approaches are resource-intensive and require different approaches for different organisms. Environmental DNA (eDNA) extracted from water samples is an efficient and versatile approach to detecting aquatic animals. In the ocean, eDNA composition reflects local fauna at fine spatial scales, but little is known about the effectiveness of eDNA-based monitoring of marine communities at larger scales. We investigated the potential of eDNA...

Data from: Temperature shapes opposing latitudinal gradients of plant taxonomic and phylogenetic β diversity

Ian R. McFadden, Brody Sandel, Constantinos Tsirogiannis, Naia Morueta-Holme, Jens-Christian Svenning, Brian J. Enquist & Nathan J. B. Kraft
Latitudinal and elevational richness gradients have received much attention from ecologists but there is little consensus on underlying causes. One possible proximate cause is increased levels of species turnover, or β diversity, in the tropics compared to temperate regions. Here, we leverage a large botanical dataset to map taxonomic and phylogenetic β diversity, as mean turnover between neighboring 100 × 100 km cells, across the Americas and determine key climatic drivers. We find taxonomic and...

Data from: Sexual conflict and intrasexual polymorphism promote assortative mating and halts population differentiation

Lars L. Iversen, Erik I. Svensson, Søren T. Christensen, Johannes Bergsten & Kaj Sand-Jensen
Sexual conflict is thought to be an important evolutionary force in driving phenotypic diversification, population divergence and speciation. However, empirical evidence is inconsistent with the generality of sexual conflict as enhancing population divergence. Here we demonstrate an alternative evolutionary outcome in which sexual conflict plays a conservative role in maintaining male and female polymorphisms locally, rather than promoting population divergence. In diving beetles, female polymorphisms have evolved in response to male mating harassment and sexual...

Data from: A Western route of prehistoric human migration from Africa into the Iberian Peninsula

Gloria Gonzalez-Fortes, Francesca Tassi, Emiliano Trucchi, Kirstin Henneberger, Johanna L.A. Paijmans, Daviz Diez-Del-Molino, Hannes Schroeder, Cecilio Barroso, Francisco J. Bermudez, Cecilio Barroso-Medina, Ana M.S. Bettencourt, Hugo A. Sampaio, Aurora Grandal-D'Anglade, Antonio Salas, Arturo De Lombera-Hermida, Ramon Fabregas, Manuel Vaquero, Susana Alonso, Maria Lozano, Xavier Rodríguez-Alvarez, Carlos Fernández-Rodríguez, Andrea Manica, Michael Hofreiter & Guido Barbujani
Being at the Western fringe of Europe, Iberia had a peculiar prehistory and a complex pattern of Neolithization. A few studies, all based on modern populations, reported the presence of DNA of likely African origin in this region, generally concluding it was the result of recent gene flow, probably during the Islamic period. Here we provide evidence of much older gene flow from Africa to Iberia by sequencing whole genomes from four human remains from...

Data from: Reduced signal for polygenic adaptation of height in UK Biobank

Jeremy J. Berg, Arbel Harpak, Nasa Sinnott-Armstrong, Anja Moltke Joergensen, Hakhamanesh Mostafavi, Yair Field, Evan August Boyle, Xinjun Zhang, Fernando Racimo, Jonathan K. Pritchard & Graham Coop
Several recent papers have reported strong signals of selection on European polygenic height scores. These analyses used height effect estimates from the GIANT consortium and replication studies. Here, we describe a new analysis based on the the UK Biobank (UKB), a large, independent dataset. We find that the signals of selection using UKB effect estimates are strongly attenuated or absent. We also provide evidence that previous analyses were confounded by population stratification. Therefore, the conclusion...

Data from: Evidence for locally adaptive metabolic rates among ant populations along an elevation gradient

Jonathan Zvi Shik, Xavier Arnan, Cristela S. Oms, Xim Cerda & Raphäel Boulay
1. As global temperatures rise, the mechanistic links between temperature, physiology and behavior will increasingly define predictions of ecological change. However, for many taxa, we currently lack consensus about how thermal performance traits vary within and across populations, and whether and how locally adaptive trait plasticity can buffer warming effects. 2. The metabolic cold adaptation hypothesis posits that cold environments (e.g. high elevations and latitudes) select for high metabolic rates (MR), even after controlling for...

Data from: Preserved collagen reveals species identity in archaeological marine turtle bones from Caribbean and Florida sites

Michael Buckley, Virginia L. Harvey, Michelle J. LeFebvre, Susan D. DeFrance, Casper Toftgaard & Andrew C. Kitchener
Advancements in molecular science are continually improving our understanding of marine turtle biology and evolution. However, there are still considerable gaps in our understanding, such as past marine turtle distributions, which can benefit from advanced zooarchaeological analyses. Here we apply collagen fingerprinting to 130 archaeological marine turtle bone samples up to 2500 years old from the Caribbean and Florida’s Gulf Coast for faunal identification, finding the vast majority of samples (88%) to contain preserved collagen...

Data from: Amphibian functional diversity is related to high annual precipitation and low precipitation seasonality in the New World

Leticia Margarita Ochoa-Ochoa, Nancy R. Mejía-Domínguez, Julian A. Velasco, Katharine Ann Marske & Carsten Rahbek
Aim. We examined the geographical distribution of functional diversity for American Amphibians and tested the relationship between functional diversity and environment. We also explored how the functional evenness of life history traits varies across biogeographical regions. Location. Continental Americas. Methods. We performed a trait classification based on an Eltonian approach and calculated functional diversity using Hill numbers, Shannon and Gini-Simpson indices. We tested the relationship between functional diversity and different axes of environmental variation by...

Data from: Sequestration and biosynthesis of cyanogenic glucosides in passion vine butterflies and consequences for the diversification of their host plants

Érika C. Pinheiro De Castro, Mika Zagrobelny, Juan Pablo Zurano, Márcio Zikan Cardoso, René Feyereisen & Søren Bak
The colorful heliconiine butterflies are distasteful to predators due to their content of defense compounds called cyanogenic glucosides (CNglcs), which they biosynthesize from aliphatic amino acids. Heliconiine larvae feed exclusively on Passiflora plants where ~30 kinds of CNglcs have been reported. Among them, some CNglcs derived from cyclopentenyl glycine can be sequestered by some Heliconius species. In order to understand the evolution of biosynthesis and sequestration of CNglcs in these butterflies and its consequences for...

Spectral data for quaking aspen (Populus tremuloides) clones of different ploidy levels

B. Blonder, B.J. Graae, B. Greer, M. Haagsma, K. Helsen, R.E. Kapás, H. Pai, J. Rieksta, D. Sapena, C.J. Still & R. Strimbeck
Data comprise measurements of spectral reflectance for quaking aspen (Populus tremuloides Michx.) trees at a range of sites in southwestern Colorado near the town of Crested Butte. Spectra were measured in three different ways: hyperspectral measurements of leaves, hyperspectral measurements of bark, and multispectral measurements of canopies. The first two measurements were made using a handheld spectrometer, while the latter were made via airborne imaging from an unmanned aerial system. In addition to these reflectance...

Extracting physiological information in experimental biology via Eulerian video magnification

Henrik Lauridsen, Selina Gonzales, Daniela Hedwig, Kathryn L. Perrin, Catherine J.A. Williams, Peter H. Wrege, Mads F. Bertelsen, Michael Pedersen & Jonathan T. Butcher
Background: Videographic material of animals can contain inapparent signals, such as color changes or motion that hold information about physiological functions, such as heart and respiration rate, pulse wave velocity and vocalization. Eulerian video magnification allows enhancement of such signals to enable their detection. The purpose of this study is to demonstrate how signals relevant to experimental physiology can be extracted from non-contact videographic material of animals. Results: We applied Eulerian video magnification to detect...

Extensive paraphyly in the typical owl family (Strigidae)

Jessie F Salter, Carl H Oliveros, Peter A Hosner, Joseph D Manthey, Mark B Robbins, Robert G Moyle, Robb T Brumfield & Brant C Faircloth
The typical owl family (Strigidae) comprises 194 species in 28 genera, 14 of which are monotypic. Relationships within and among genera in the typical owls have been challenging to discern because mitochondrial data have produced equivocal results and because many monotypic genera have been omitted from previous molecular analyses. Here, we collected and analyzed DNA sequences of ultraconserved elements (UCEs) from 43 species of typical owls to produce concatenated and multispecies coalescent-based phylogenetic hypotheses for...

Data from: Apolipoprotein M-bound sphingosine-1-phosphate regulates blood-brain barrier paracellular permeability and transcytosis

Mette Mathiesen Janiurek, Rana Soylu-Kucharz, Christina Christoffersen, Krzysztof Kucharz & Martin Lauritzen
The blood-brain barrier (BBB) is formed by the endothelial cells lining cerebral microvessels. Here, we report that the BBB permeability is modified by apolipoprotein M (apoM)-bound sphingosine 1–phosphate (S1P). We used two-photon microscopy to monitor changes in BBB permeability in apoM-deficient mice (apoM-/-), showing significant increases in paracellular BBB permeability to small molecules without structural changes in junctional complexes between endothelial cells. Lack of apoM-bound S1P increased vesicle-mediated transfer of albumin across endothelium of brain...

Data from: Monitoring a Norwegian freshwater crayfish tragedy - eDNA snapshots of invasion, infection and extinction

David A. Strand, Stein Ivar Johnsen, Johannes C. Rusch, Sune Agersnap, William Brenner Larsen, Steen Wilhelm Knudsen, Peter Rask Møller & Trude Vrålstad
1.The European Noble crayfish (Astacus astacus) is threatened by crayfish plague caused by the oomycete Aphanomyces astaci, which is spread by the invasive North American crayfish (e.g. signal crayfish, Pacifastacus leniusculus). Surveillance of crayfish plague status in Norway has traditionally relied on the monitoring survival of cage‐held noble crayfish, a method of ethical concern. Additionally, trapping is used in crayfish population surveillance. Here we test whether environmental DNA (eDNA) monitoring could provide a suitable alternative...

Supporting data for Formation of Highly Oxidized Molecules from NO3 Radical Oxidation of Δ-3-Carene: A Computational Mechanism

Danielle Draper, Nanna Myllys, Noora Hyttinen, Kristian Moller, Henrik Kjaergaard, Juliane Fry, James Smith & Theo Kurten
NO3 radical oxidation of most monoterpenes is a significant source of secondary organic aerosol (SOA) in many regions influenced by both biogenic and anthropogenic emissions, but there are very few published mechanistic studies of NO3 chemistry beyond simple 1st generation products. Here, we present a computationally-derived mechanism detailing the unimolecular pathways available to the 2nd generation of peroxy radicals following NO3 oxidation of Δ-3-carene, defining generations based on the sequence of peroxy radicals formed rather...

Data from: Quantification of the activity of detoxifying enzymes in terrestrial invertebrates: optimization, evaluation and use of in vitro and ex vivo methods

Kathrine Eggers Pedersen, Brian Lund Fredensborg, Annette Bruun Jensen & Nina Cedergreen
1. The negative effects of pesticides on beneficial terrestrial invertebrates have received much attention, and in this context quantification of the activity of metabolic enzymes provides an important tool. Unfortunately, current methodologies are limited in their use. 2. In this study, the grain beetle, Tenebrio molitor was used to illustrate why in vitro methods are ineffective to quantify metabolic enzymes in some insects. It was further used to optimize an ex vivo assay that can...

Data from: Neuromuscular adverse events associated with Anti-PD-1 monoclonal antibodies: systematic review

Annette Johansen, Søren Just Christensen, David Scheie, Joan Lilja Sunnleyg Højgaard & Daniel Kondziella
Neuromuscular adverse events following cancer treatment with anti–programmed cell death protein 1 (PD-1) monoclonal antibodies are relatively rare, yet potentially fatal. We performed a systematic review to characterize the clinical presentation, diagnostic workup, and management of neuromuscular disorders (NMDs) in patients treated with nivolumab or pembrolizumab monotherapy or concurrent with other immunologic agents, such as ipilimumab. Sixty-one publications on 85 patients (mean age 66.9 years [range 34–86]; male/female 2.6:1; 59% metastatic melanoma) were identified from...

Data from: Red and orange flags for secondary headaches in clinical practice: SNNOOP10 list

Thien Phu Do, Angelique Remmers, Henrik Winther Schytz, Christoph Schankin, Sarah E. Nelson, Mark Obermann, Jakob Møller Hansen, Alexandra J. Sinclair, Andreas R. Ganteinbein & Guus G. Schoonman
A minority of headache patients have a secondary headache disorder. The medical literature presents and promotes red flags to increase the likelihood of identifying a secondary etiology. In this review, we aim to discuss the incidence and prevalence of secondary headaches as well as the data on sensitivity, specificity, and predictive value of red flags for secondary headaches. We review the following red flags: (1) systemic symptoms including fever; (2) neoplasm history; (3) neurologic deficit...

Data from: Palaeoproteomics resolves sloth phylogeny

Samantha Presslee, Graham J. Slater, Francois Pujos, Analia M. Forasiepi, Roman Fischer, Kelly Molloy, Meaghan Mackie, Jesper V. Olsen, Alejandro Kramarz, Matias Taglioretti, Fernando Scaglia, Maximiliano Lezcano, José Luis Lanata, John Southon, Robert Feranec, Jonathan Bloch, Adam Hajduk, Fabiana M. Martin, Rodolfo Salas Gismondi, Marcelo Reguero, Christian De Muizon, Alex Greenwood, Brian T. Chait, Kirsty Penkman, Matthew Collins … & Ross D. E. MacPhee
The living tree sloths Choloepus and Bradypus are the only remaining members of Folivora, a major xenarthran radiation that occupied a wide range of habitats in many parts of the western hemisphere during the Cenozoic, including both continents and the West Indies. Ancient DNA evidence has played only a minor role in folivoran systematics, as most sloths lived in places not conducive to genomic preservation. Here we utilize collagen sequence information, both separately and in...

Data from: Natural history of limb girdle muscular dystrophy R9 over 6 years: searching for trial endpoints

Alexander P. Murphy, Jasper Morrow, Julia R. Dahlqvist, Tanya Stojkovic, Tracey A. Willis, Christopher D. J. Sinclair, Stephen Wastling, Tarek Yousry, Michael S. Hanna, Meredith K. James, Anna Mayhew, Michelle Eagle, Laurence E. Lee, Jean-Yves Hogrel, Pierre G. Carlier, John S. Thornton, John Vissing, Kieren G. Hollingsworth & Volker Straub
Objective: Limb girdle muscular dystrophy type R9 (LGMD R9) is an autosomal recessive muscle disease for which there is currently no causative treatment. The development of putative therapies requires sensitive outcome measures for clinical trials in this slowly progressing condition. This study extends functional assessments and MRI muscle fat fraction measurements in an LGMD R9 cohort across 6 years. Methods: Twenty‐three participants with LGMD R9, previously assessed over a 1‐year period, were re‐enrolled at 6...

Catchment properties and the photosynthetic trait composition of freshwater plant communities

Lars Lønsmann Iversen, A. Winkel, L. Baastrup-Spohr, A. B. Hinke, J. Alahuhta, A. Baattrup-Pedersen, S. Birk, P. Brodersen, P. A. Chambers, F. Ecke, T. Feldmann, D. Gebler, J. Heino, T. S. Jespersen, S. J. Moe, T. Riis, L. Sass, O. Vestergaard, S. C. Maberly, K. Sand-Jensen & O. Pedersen
Unlike in land plants, photosynthesis in many aquatic plants relies on bicarbonate in addition to carbon dioxide (CO2) to compensate for the low diffusivity and potential depletion of CO2 in water. Concentrations of bicarbonate and CO2 vary greatly with catchment geology. In this study, we investigate whether there is a link between these concentrations and the frequency of freshwater plants possessing the bicarbonate use trait. We show, globally, that the frequency of plant species with...

Data from: Adaptive radiation and the evolution of nectarivory in a large songbird clade

Petter Zahl Marki, Jonathan D. Kennedy, Chris Cooney, Carsten Rahbek & Jon Fjeldså
The accumulation of exceptional ecological diversity within a lineage is a key feature of adaptive radiation resulting from diversification associated with the subdivision of previously underutilized resources. The invasion of unoccupied niche space is predicted to be a key determinant of adaptive diversification, and this process may be particularly important if the diversity of competing lineages within the area, in which the radiation unfolds, is already high. Here, we test whether the evolution of nectarivory...

Data from: Tropical forest type influences community assembly processes in arbuscular mycorrhizal fungi

Camilla M.R. Pereira, Álvaro López-García, Danielle Karla Alves Da Silva, Leonor C. Maia, Tobias G. Frøslev, Rasmus Kjøller & Søren Rosendahl
Aim: Plant community assembly in tropical rainforest has been shown to be largely governed by stochastic processes, but as arbuscular mycorrhizal (AM) fungi display limited host preference, they may not follow the same stochastic assembly pattern. Here, we determined the relative importance of environmental and spatial drivers responsible for the community assembly process of AM fungi in two types of tropical rainforest (semideciduous rainforest and dense ombrophilous forests). Location: Atlantic rainforest in northeastern Brazil, South...

Data from: A consistent species richness-climate relationship for oaks across the Northern Hemisphere

Xiaoting Xu, Dimitar Dimitrov, Nawal Shrestha, Carsten Rahbek & Zhiheng Wang
Aim: Although the effects of climate on species richness are known, regional processes may lead to different species richness-climate relationships across continents resulting in species richness anomalies, especially for tropical groups. Phylogenetic niche conservatism may also influence species richness-climate relationships of different lineages. Here, we tested whether regional effects also exist for temperate lineages using the genus Quercus. Location: Northern Hemisphere Time period: Present day Major taxa studied: Quercus (Fagaceae) Methods: We used a dated...

Registration Year

  • 2019

Resource Types

  • Dataset


  • University of Copenhagen
  • Aarhus University
  • Lund University
  • Florida Museum of Natural History
  • University of California, Irvine
  • University of Duisburg-Essen
  • Swedish Museum of Natural History
  • Bernardino Rivadavia Natural Sciences Museum
  • University of Kansas
  • Bangor University