454 Works

Data from: Metabarcoding of soil environmental DNA replicates plant community variation but not specificity

Tobias Frøslev & Christopher Barnes
While metabarcoding of plant DNA from their environment is an exciting method that can supplement inventorying of live plant species, the accuracy and specificity has yet to be fully assessed over complex continuous landscapes. In this work, we evaluate plant community profiles produced via metabarcoding of soil by comparing them to a morphological survey. We assessed plant communities by metabarcoding of soil DNA in 130 sites along ecological gradients (nutrients, succession, moisture) in Denmark using...

Herpetofaunal diversity changes with climate: evidence from the Quaternary of McEachern’s Deathtrap Cave, southeastern Australia

Till Ramm, Kailah M. Thorn, Christy A. Hipsley, Johannes Müller, Scott Hocknull & Jane Melville
The Quaternary Period is characterized by dramatic global climatic changes. Quaternary fossil deposits, which can offer excellent stratigraphic resolution, provide a unique opportunity to understand how fauna respond to past environmental change. Here, we test if the herpetofauna of McEachern’s Deathtrap Cave, a late Pleistocene to Holocene pitfall trap deposit from Victoria, Australia, shows climate-related shifts in taxonomic relative abundance through time. During the last 14,000 years, southeastern Australia experienced pronounced periods of aridity, while...

Older patients’ perspectives on participating in multimodal rehabilitation including anabolic steroids following hip fracture: a qualitative study embedded within a pilot RCT

Signe Hulsbæk, Louise Bolvig Laursen, Morten Tange Kristensen & Julie Midtgaard
To explore patient perspectives of participating in a pilot RCT evaluating feasibility and effect of anabolic steroid, physiotherapy and nutritional supplement following hip fracture. Semi-structured telephone-based interviews of 16 women and three men (average age 73 years) were conducted at baseline and after a 12-week intervention. Qualitative content analysis was performed. Two main categories were identified: (1) Trust and hope for a positive change with three sub-categories; reflections on anabolic steroids, anticipation of extra attention,...

Simple attributes predict the value of plants as hosts to fungal and arthropod communities

Hans Henrik Bruun, Ane Kirstine Brunbjerg, Camilla Fløjgaard, Lars Dalby, Tobias Guldberg Frøslev, Simon Haarder, Jacob Heilmann-Clausen, Toke Hoye, Thomas Læssøe & Rasmus Ejrnæs
Fungal and arthropod consumers constitute the vast majority of global terrestrial biodiversity. Yet, the link from richness and composition of producer (plant) communities to the richness of consumer communities is poorly understood. Fungal and arthropod species richness could be a simple function of producer species richness at a site. Alternatively, it could be a complex function of chemical and structural properties of the producer species making up communities. We used databases on plant-fungus and plant-arthropod...

Guide-Entrepreneurs Developing Urban Ecotourism

Jane Widtfeldt Meged & Berit Charlotte Kaae

A national scale BioBlitz using citizen science and eDNA metabarcoding for monitoring coastal marine fish

Sune Agersnap, Eva Egelyng Sigsgaard, Mads Reinholdt Jensen, Marcelo De Paula Avila, Henrik Carl, Peter Rask Møller, Simon Leed Krøs, Steen Wilhelm Knudsen, Mary Wisz & Philip Francis Thomsen
Marine biodiversity is threatened by human activities. To understand the changes happening in aquatic ecosystems and to inform management, detailed, synoptic monitoring of biodiversity across large spatial extents is needed. Such monitoring is challenging due to the time, cost, and specialized skills that this typically requires. In an unprecedented study, we here combined citizen science with eDNA metabarcoding to map coastal fish biodiversity at a national scale. We engaged 360 citizen scientists to collect filtered...

Apurinic/apyrimidinic endonuclease triggered doxorubicin-releasing DNA nanoprism for target therapy

Meng Liang, Na Li, Fei Liu, Nan Zeng, Changyuan Yu & Shuo Li
Drug delivery and triggered release in tumor cells would realize the ultimate goal of precise cancer treatment. An APE1 triggered DNA nanoprism was designed, aiming at the applications of both drug delivery and precise triggered drug release in cancer cell. We demonstrate that the AP-Prism was successfully used as a vehicle based on the intracellular endogenous enzyme APE1 triggered for controlled drug delivery and triggered release. The box like DNA prism was self-assembled by annealing...

Short-term temporal variation of coastal marine eDNA

Mads Reinholdt Jensen, Eva Egelyng Sigsgaard, Marcelo De Paula Ávila, Sune Agersnap, William Brenner-Larsen, Mita Eva Sengupta, Yingchun Xing, Marcus Anders Krag, Steen Wilhelm Knudsen, Henrik Carl, Peter Rask Møller & Philip Francis Thomsen
Temporal variation in eDNA signals is increasingly explored for understanding community ecology in aquatic habitats. Seasonal changes have been addressed using eDNA sampling, but very little is known regarding short-term temporal variation that spans hours to days. To address this, we filtered marine water samples from a single coastal site in Denmark every hour for 32 h. We used metabarcoding to target both fish and broader eukaryote diversity and evaluated temporal changes in this marine...

Reproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts

Steffan Noe Christiansen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Jing Liu, Mikkel Meyer Andersen, Jacob Tfelt-Hansen & Niels Morling
The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson’s...

Hvor meget lignede neandertalerne os?

Paige Madison, Trine Kellberg Nielsen & Peter C. Kjærgaard

Data from: Ecological adaptation and birdsong: how body and bill sizes affect passerine sound frequencies

Gonçalo C. Cardoso, Jakob Isager Friis, Joana Sabino, Pedro Santos & Torben Dabelsteen
The avian bill is finely adjusted to foraging ecology and, as part of the vocal tract, it may also affect sexual signals such as songs. Acoustic theory predicts that larger bills lower the resonant frequency of vocal tracts, allowing larger-billed species to emphasize lower sound frequencies. Theory also predicts that larger-billed species can change bill gape so as to sing over a wider frequency bandwidth. We tested these associations between bill size and sound frequencies...

Ecological data on evergreen forest types of central plains Cambodia

Ida Theilade, Henrik Meilby & Katrine Gro Friborg
Four different lowland evergreen forest types, each with a unique species composition and ecological characteristics is described from the central plains, Cambodia. Through six botanical expeditions, we collected data on the forest types using 50x10 = 500 sqm strip sample plots (n=119); i) riverine forest dominated by Dipterocarpus costatus (Dipterocarpaceae): ii) tall dipterocarp forest dominated by Anisoptera costata (Dipterocarpaceae); iii) swamp forest dominated by Macaranga triloba (Euphorbiaceae); and iv) “Sralao” forest with a monodominance of...

Childhood-onset retinal dystrophies reduces life-time income by one third - an individual based socio-economic analysis

Line Kessel, Jakob Kjellberg, Kamilla Nissen, Anette Rasmussen & Morten la Cour
The aim of this study was to evaluate lifetime income, educational level and workforce participation in patients with childhood-onset inherited retinal dystrophies (IRD). The registry-based study using national, Danish databases on education, income, employment and social benefits in a cohort of 515 patients with childhood-onset IRD and without severe systemic comorbidities matched 1:4 to an age- and sex to a control sample of the Danish background population. Socio-economic status was modelled with focus on grade...

Childhood-onset retinal dystrophies reduces life-time income by one third - an individual based socio-economic analysis

Line Kessel, Jakob Kjellberg, Kamilla Nissen, Anette Rasmussen & Morten la Cour
The aim of this study was to evaluate lifetime income, educational level and workforce participation in patients with childhood-onset inherited retinal dystrophies (IRD). The registry-based study using national, Danish databases on education, income, employment and social benefits in a cohort of 515 patients with childhood-onset IRD and without severe systemic comorbidities matched 1:4 to an age- and sex to a control sample of the Danish background population. Socio-economic status was modelled with focus on grade...

COVID-19: Hvem skal nu vaccineres og hvorfor? Professor giver overblikket

Camilla Foged

Additional file 1 of Family-based cognitive behavioural therapy versus family-based relaxation therapy for obsessive-compulsive disorder in children and adolescents (the TECTO trial): a statistical analysis plan for the randomised clinical trial

Markus Harboe Olsen, Julie Hagstrøm, Nicole Nadine Lønfeldt, Camilla Uhre, Valdemar Uhre, Linea Pretzmann, Sofie Heidenheim Christensen, Christine Thoustrup, Nicoline Løcke Jepsen Korsbjerg, Anna-Rosa Cecilie Mora-Jensen, Melanie Ritter, Janus Engstrøm, Jane Lindschou, Hartwig Roman Siebner, Frank Verhulst, Pia Jeppesen, Jens Richardt Møllegaard Jepsen, Signe Vangkilde, Per Hove Thomsen, Katja Hybel, Line Katrine Harder Clemmesen, Christian Gluud, Kerstin Jessica Plessen, Anne Katrine Pagsberg & Janus Christian Jakobsen
Additional file 1: Table 1. Participant characteristics. Figure 1. CONSORT flow diagram. Figure 2. Psychopathology and family burden. Figure 3. Response status at 16 weeks. Figure 4. Negative effects questionnaire. Supplemental Table 1. Detailed comorbidities. Supplemental Table 2. Psychopathology and family burden. Supplemental Table 3. Kidscreen-52. Supplemental Timeline. Supplemental Assumptions.

Additional file 1 of The changing spectrum of microbial aetiology of respiratory tract infections in hospitalized patients before and during the COVID-19 pandemic

Sondre Serigstad, Dagfinn L. Markussen, Christian Ritz, Marit H. Ebbesen, Siri T. Knoop, Øyvind Kommedal, Lars Heggelund, Elling Ulvestad, Rune O. Bjørneklett & Harleen M. S. Grewal
Additional file 1. BioFire® FilmArray® Pneumonia panel plus (FAP plus) targets.

Additional file 3 of Microscopic marine invertebrates are reservoirs for cryptic and diverse protists and fungi

Corey C. Holt, Vittorio Boscaro, Niels W. L. Van Steenkiste, Maria Herranz, Varsha Mathur, Nicholas A. T. Irwin, Gracy Buckholtz, Brian S. Leander & Patrick J. Keeling
Additional file 3: Supplementary Figure 2. Ciliophora phylogeny with sequence labels and UltraFast bootstrap support values. Maximum-likelihood phylogeny of all Ciliophora ASVs, reference sequences, and BLAST hits using the GTR+F+R7 substitution model. Individual ASVs indicated by bold tip labels and white rectangles in grey ring. Accompanying dots reflect presence in each host phylum (coloured accordingly).

Additional file 1 of Colorectal cancer incidences in Lynch syndrome: a comparison of results from the prospective lynch syndrome database and the international mismatch repair consortium

Pål Møller, Toni Seppälä, James G. Dowty, Saskia Haupt, Mev Dominguez-Valentin, Lone Sunde, Inge Bernstein, Christoph Engel, Stefan Aretz, Maartje Nielsen, Gabriel Capella, Dafydd Gareth Evans, John Burn, Elke Holinski-Feder, Lucio Bertario, Bernardo Bonanni, Annika Lindblom, Zohar Levi, Finlay Macrae, Ingrid Winship, John-Paul Plazzer, Rolf Sijmons, Luigi Laghi, Adriana Della Valle, Karl Heinimann … & Mark A. Jenkins
Additional file 1.

Trajectories of psychosocial symptoms and wellbeing in asylum seekers and refugees exposed to traumatic events and resettled in Western Europe, Turkey, and Uganda

Marianna Purgato, Federico Tedeschi, Giulia Turrini, Ceren Acartürk, Minna Anttila, Jura Augustinavicious, Josef Baumgartner, Richard Bryant, Rachel Churchill, Zeynep Ilkkursun, Eirini Karyotaki, Thomas Klein, Markus Koesters, Tella Lantta, Marx R. Leku, Michela Nosè, Giovanni Ostuzzi, Mariana Popa, Eleonora Prina, Marit Sijbrandij, Ersin Uygun, Maritta Välimäki, Lauren Walker, Johannes Wancata, Ross G. White … & Corrado Barbui
Background: Longitudinal studies examining mental health trajectories in refugees and asylum seekers are scarce. Objectives: To investigate trajectories of psychological symptoms and wellbeing in refugees and asylum seekers, and identify factors associated with these trajectories. Method: 912 asylum seekers and refugees from the control arm of three trials in Europe (n = 229), Turkey (n = 320), and Uganda (n = 363) were included. We described trajectories of psychological symptoms and wellbeing, and used trauma...

Additional file 3 of Blood flow restriction added to usual care exercise in patients with early weight bearing restrictions after cartilage or meniscus repair in the knee joint: a feasibility study

Thomas Linding Jakobsen, Kristian Thorborg, Jakob Fisker, Thomas Kallemose & Thomas Bandholm
Additional file 3: S3. Rehabilitation regimens - Cartilage (Steadman procedure) or meniscus repair.

Additional file 4 of Blood flow restriction added to usual care exercise in patients with early weight bearing restrictions after cartilage or meniscus repair in the knee joint: a feasibility study

Thomas Linding Jakobsen, Kristian Thorborg, Jakob Fisker, Thomas Kallemose & Thomas Bandholm
Additional file 4: S4. Blood Flow Restriction Exercise Leaflet.

Additional file 4 of Blood flow restriction added to usual care exercise in patients with early weight bearing restrictions after cartilage or meniscus repair in the knee joint: a feasibility study

Thomas Linding Jakobsen, Kristian Thorborg, Jakob Fisker, Thomas Kallemose & Thomas Bandholm
Additional file 4: S4. Blood Flow Restriction Exercise Leaflet.

Additional file 5 of Blood flow restriction added to usual care exercise in patients with early weight bearing restrictions after cartilage or meniscus repair in the knee joint: a feasibility study

Thomas Linding Jakobsen, Kristian Thorborg, Jakob Fisker, Thomas Kallemose & Thomas Bandholm
Additional file 5: S5. Usual care exercise after cartilage or meniscus repair in the knee joint - week 3–6 postoperatively.

Identification of a window of androgen sensitivity for somatic cell function in human fetal testis cultured ex vivo

Malene Lundgaard Riis, Gabriele Matilionyte, John E. Nielsen, Cecilie Melau, David Greenald, Kristine Juul Hare, Lea Langhoff Thuesen, Eva Dreisler, Kasper Aaboe, Pia Tutein Brenøe, Anna-Maria Andersson, Jakob Albrethsen, Hanne Frederiksen, Ewa Rajpert-De Meyts, Anders Juul, Rod T. Mitchell & Anne Jørgensen
Abstract Background Reduced androgen action during early fetal development has been suggested as the origin of reproductive disorders comprised within the testicular dysgenesis syndrome (TDS). This hypothesis has been supported by studies in rats demonstrating that normal male development and adult reproductive function depend on sufficient androgen exposure during a sensitive fetal period, called the masculinization programming window (MPW). The main aim of this study was therefore to examine the effects of manipulating androgen production...

Registration Year

  • 2022
    454

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    227
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    105
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    91
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    26
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    3
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    1
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    1

Affiliations

  • University of Copenhagen
    454
  • Copenhagen University Hospital
    114
  • Rigshospitalet
    89
  • Aarhus University
    56
  • University of Southern Denmark
    42
  • Amager Hospital
    28
  • Shanghai Jiao Tong University
    26
  • Chongqing Medical University
    24
  • Norwegian University of Science and Technology
    24
  • Erasmus MC
    19