3 Works

Data from: Extensive gene flow over Europe and possible speciation over Eurasia in the ectomycorrhizal basidiomycete Laccaria amethystina complex.

Lucie Vincenot, Kazuhide Nara, Christopher Sthultz, Jessy Labbé, Marie-Pierre Dubois, Leho Tedersoo, Francis Martin & Marc-André Selosse
Biogeographic patterns and large-scale genetic structure have been little studied in ectomycorrhizal fungi, despite the ecological and economic importance of ectomycorrhizal symbioses. We coupled population genetics and phylogenetic approaches to understand spatial structure in fungal populations on a continental scale. Using 9 microsatellite markers, we characterised gene flow among 16 populations of the widespread ectomycorrhizal basidiomycete Laccaria amethystina over Europe (over 2900km). We also widened our scope to two additional populations from Japan (104 km...

Data from: The genetic structure of the plant pathogenic fungus Melampsora larici-populina on its wild host is extensively impacted by host domestication

Constance Xhaard, Bénédicte Fabre, Axelle Andrieux, Pierre Gladieux, Benoit Barrès, Pascal Frey & Fabien Halkett
Wild and cultivated plants represent very different habitats for pathogens, especially when cultivated plants bear qualitative resistance genes. Here, we investigated to what extent the population genetic structure of a plant pathogenic fungus collected on its wild host can be impacted by the deployment of resistant cultivars. We studied one of the main poplar diseases, poplar rust, caused by the fungus Melampsora larici-populina. A thousand fifty individuals sampled from several locations in France were phenotyped...

Data from: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries

Thibaut Malausa, André Gilles, Emese Meglécz, Hélène Blanquart, Stéphanie Duthoy, Caroline Costedoat, Vincent Dubut, Nicolas Pech, Philippe Castagnone-Sereno, Christophe Délye, Nicolas Feau, Pascal Frey, Philippe Gauthier, Thomas Guillemaud, Laurent Hazard, Valérie Le Corre, Brigitte Lung-Escarmant, Pierre-Jean G Malé, Stéphanie Ferreira & Jean-François Martin
Microsatellites (or SSR: simple sequence repeat) are among the most frequently used DNA markers in many areas of research. The use of microsatellite markers is limited by the difficulties involved in their de novo isolation from species for which no genomic resources are available. We describe here a high-throughput method for isolating microsatellite markers based on coupling multiplex microsatellite enrichment and Next-Generation Sequencing on 454 GS-FLX Titanium platforms. The procedure was calibrated on a model...

Registration Year

  • 2011
    3

Resource Types

  • Dataset
    3

Affiliations

  • Interactions Arbres-Microorganismes
    3
  • Centre d'Ecologie Fonctionnelle et Evolutive
    1
  • AGroecologies, Innovations & Ruralities
    1
  • University of Paris-Sud
    1
  • French National Centre for Scientific Research
    1
  • Aix-Marseille University
    1
  • University of Tartu
    1
  • University of Tokyo
    1
  • Centre de Biologie et de Gestion des Populations
    1