610 Works

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression

Tao Deng, Yang Liu, Yanlong Yang, Leyong Yuan, Fangfang Liu, Xiaobo Wang, Qiuying Zhang & Mingshui Xie
Circular RNA circ_0000285 is differentially expressed in several malignancies; however, its role in gliomas is under investigation. Reverse transcription quantitative polymerase chain reaction was conducted to evaluate the expression of circ_0000285, miR-197-3p, and CDC28 protein kinase regulatory subunit 1B (CKS1B) in glioma tissues and cells. Cell Counting Kit-8 and Transwell invasion assays coupled with Western blotting analysis using anti-Bax and anti-Bcl-2 antibodies were performed to evaluate cell proliferation, invasion, and apoptosis. Luciferase reporter and AGO2...

Effects of glycerol and glucose on docosahexaenoic acid synthesis in Aurantiochyrium limacinum SFD-1502 by transcriptome analysis

Huaqiu Zhang, Xiangying Zhao, Chen Zhao, Jiaxiang Zhang, Yang Liu, Mingjing Yao & Jianjun Liu
Docosahexaenoic acid (DHA) has numerous functions in adjusting the organic health and pragmatic value in medicine and food field. In this study, we compared glycerol and glucose as the only carbon source for DHA production by Aurantiochytrium. When the glycerol concentration was 120 g/L, the maximum DHA yield was 11.08 g/L, and the DHA yield increased significantly, reaching 47.67% of the total lipid content. When the cells grew in glucose, the DHA proportion was 37.39%....

Role of lncRNA LINC01194 in hepatocellular carcinoma via the miR-655-3p/SMAD family member 5 axis

Yang Liu, Jie Liu, Junkai Cui, Ruolei Zhong & Guoyang Sun
Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Risk factors influencing cure of ultrasound-guided microwave ablation for primary hyperparathyroidism

Fangyi Liu, Li Zang, Yang Liu, Xiaoling Yu, Zhigang Cheng, Zhiyu Han, Jie Yu & Ping Liang
To evaluate the potential risk factors influencing cure rate of ultrasound-guided microwave ablation (MWA) for primary hyperparathyroidism (PHPT). Seventy five patients (25 males and 50 females; mean age, 56.80 ± 12.34; age range, 26–85) with PHPT undergoing MWA under ultrasound guidance were enrolled between May 2017 and December 2020. The cure rate and complications were evaluated after treatment. The potential factors influencing cure rate of ultrasound-guided MWA for PHPT were analyzed by univariate and multivariate...

Tandem mass tag-based serum proteomic profiling revealed diabetic foot ulcer pathogenesis and potential therapeutic targets

Xiao-Ting Yu, Feng Wang, Jia-Tong Ding, Bo Cai, Juan-Juan Xing, Guang-Hua Guo & Fei Guo
Diabetic foot ulcer (DFU), one of the most serious complications of diabetes mellitus, is associated with a high amputation rate and decreased life quality. The impact of blood serum proteins on the occurrence and development of DFU has attracted a lot of interest. In this study, we aimed to define and compare the serum proteome of patients with DFU and healthy control (HC) to provide new insights into DFU pathogenesis. DFU patients and age- and...

Tandem mass tag-based serum proteomic profiling revealed diabetic foot ulcer pathogenesis and potential therapeutic targets

Xiao-Ting Yu, Feng Wang, Jia-Tong Ding, Bo Cai, Juan-Juan Xing, Guang-Hua Guo & Fei Guo
Diabetic foot ulcer (DFU), one of the most serious complications of diabetes mellitus, is associated with a high amputation rate and decreased life quality. The impact of blood serum proteins on the occurrence and development of DFU has attracted a lot of interest. In this study, we aimed to define and compare the serum proteome of patients with DFU and healthy control (HC) to provide new insights into DFU pathogenesis. DFU patients and age- and...

Research Strategy for Constructing a Green Infrastructure Network Based on Spatial Prioritisation

Dongmeng Wang, Chang Liu, Yue Guo, Puxia Tang, Jie Jiao, Weihan Kong, Ling Zhang, Yiping Liu & Dezheng Kong
In 2004, the ‘Rise of Central China Plan’ was proposed to further accelerate the urbanization of China’s central region. Since 2010, the destruction of the region’s ecological system caused by urban expansion has led to the environmental deterioration and increased risks of natural disasters to which cities have become increasingly vulnerable. In this study, six districts and counties along the Yellow River in Zhengzhou were selected for inclusion in the research. A multidimensional green infrastructure...

Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation

Hainan He, Junling Wang, Xingmei Mou, Xin Liu, Qiao Li, Mingyue Zhong, Bingbing Luo, Zhisheng Yu, Jingjing Zhang, Tian Xu, Chengli Dou, Danya Wu, Wei Qing, Linhui Wu, Kai Zhou, Zhengang Fan, Tingting Wang, Taotao Hu, Xia Zhang, Jilong Zhou & Yi-Liang Miao
Macroautophagy/autophagy is a cellular and energy homeostatic mechanism that contributes to maintain the number of primordial follicles, germ cell survival, and anti-ovarian aging. However, it remains unknown whether autophagy in granulosa cells affects oocyte maturation. Here, we show a clear tendency of reduced autophagy level in human granulosa cells from women of advanced maternal age, implying a potential negative correlation between autophagy levels and oocyte quality. We therefore established a co-culture system and show that...

Metabolic profiling and pharmacokinetic studies of Baihu-Guizhi decoction in rats by UFLC-Q-TOF–MS/MS and UHPLC-Q-TRAP-MS/MS

Yan He, Zhenkun Zhou, Weijie Li, Yanqiong Zhang, Ruoyao Shi, Tao Li, Linlin Jin, Hongliang Yao, Na Lin & Hao Wu
Abstract Background Baihu-Guizhi decoction (BHGZD) is a well-documented traditional Chinese Medicine (TCM) prescription that has been extensively applied to treating rheumatoid arthritis. Despite of its beneficial outcomes, the chemical constituents of BHGZD have not been fully portrayed and the in vivo absorption, distribution, metabolism, and excretion (ADME) patterns of absorbed components have never been described. Methods Characterization of absorbed components and in vivo biotransformation profiling of these feature compounds were based on the ultra-fast liquid...

Fibroblast growth factor 10 attenuates chronic obstructive pulmonary disease by protecting against glycocalyx impairment and endothelial apoptosis

Tian Jiang, Weiping Hu, Shaoyuan Zhang, Changhao Ren, Siyun Lin, Zhenyu Zhou, Hao Wu, Jun Yin & Lijie Tan
Abstract Background The defects and imbalance in lung repair and structural maintenance contribute to the pathogenesis of chronic obstructive pulmonary diseases (COPD), yet the molecular mechanisms that regulate lung repair process are so far incompletely understood. We hypothesized that cigarette smoking causes glycocalyx impairment and endothelial apoptosis in COPD, which could be repaired by the stimulation of fibroblast growth factor 10 (FGF10)/FGF receptor 1 (FGFR1) signaling. Methods We used immunostaining (immunohistochemical [IHC] and immunofluorescence [IF])...

Additional file 1 of Inner and outer setting factors that influence the implementation of the National Diabetes Prevention Program (National DPP) using the Consolidated Framework for Implementation Research (CFIR): a qualitative study

Lillian Madrigal, Olivia C. Manders, Michelle Kegler, Regine Haardörfer, Sarah Piper, Linelle M. Blais, Mary Beth Weber & Cam Escoffery
Additional file 1: Table 1. Interview Guide Questions.

Supplemental Material - Predictors of Discussing Lung Cancer Screening with a Health Care Provider Among Current and Former Smokers in HINTS: A Secondary Data Analysis

Shacoria L. Anderson, Melvin D. Livingston, Kristin A. Higgins & Colleen M. McBride
Supplemental Material for Predictors of Discussing Lung Cancer Screening with a Health Care Provider Among Current and Former Smokers in HINTS: A Secondary Data Analysis by Shacoria L. Anderson, MPH, Melvin D. Livingston, PhD, Kristin A. Higgins, MD, and Colleen M. McBride, PhD in Cancer Control

Predictors of Discussing Lung Cancer Screening with a Health Care Provider Among Current and Former Smokers in HINTS: A Secondary Data Analysis

Shacoria L. Anderson, Melvin D. Livingston, Kristin A. Higgins & Colleen M. McBride
IntroductionLung cancer screening is greatly underutilized among those who may benefit from early detection.MethodsWe analysed data from a subsample (n = 929) of the 2020 Health Information National Trends Survey. We tested multivariable logistic regression models of associations of cancer worry, information insufficiency, and perceived information gathering capacity with reports of having discussed lung cancer screening with a health care provider.ResultsAmong former smokers, no factors were associated significantly with lung cancer screening information seeking. However,...

One-step fabrication of lidocaine/CalliSpheres® composites for painless transcatheter arterial embolization

Chuan Tian, Zijian Wang, Lei Huang, Yimin Liu, Kunpeng Wu, Zhaonan Li, Bin Han, Dechao Jiao, Xinwei Han & Yanan Zhao
Abstract Background Transcatheter arterial embolization (TAE) is one of the first-line treatments for advanced hepatocellular cancer. The pain caused by TAE is a stark complication, which remains to be prevented by biomedical engineering methods. Methods Herein, a commercial embolic agent CalliSpheres® bead (CB) was functionally modified with lidocaine (Lid) using an electrostatic self-assembly technique. The products were coded as CB/Lid-n (n = 0, 5, 10, corresponding to the relative content of Lid). The chemical compositions,...

From Black Box to Shining Spotlight: Using Random Forest Prediction Intervals to Illuminate the Impact of Assumptions in Linear Regression

Andrew J. Sage, Yang Liu & Joe Sato
We introduce a pair of Shiny web applications that allow users to visualize random forest prediction intervals alongside those produced by linear regression models. The apps are designed to help undergraduate students deepen their understanding of the role that assumptions play in statistical modeling by comparing and contrasting intervals produced by regression models with those produced by more flexible algorithmic techniques. We describe the mechanics of each approach, illustrate the features of the apps, provide...

Additional file 1 of Neuroinflammatory transcriptional programs induced in rhesus pre-frontal cortex white matter during acute SHIV infection

Chase E. Hawes, Sonny R. Elizaldi, Danielle Beckman, Giovanne B. Diniz, Yashavanth Shaan Lakshmanappa, Sean Ott, Blythe P. Durbin-Johnson, Ashok R. Dinasarapu, Andrea Gompers, John H. Morrison & Smita S. Iyer
Additional file 1: Fig. S1. Ranked genes by median normalized read counts in units of Log2 counts per million (Log2CPM) in the subcortical white matter of the pre-frontal cortex (PFCw), and gray matter of the superior temporal sulcus (STS), caudate nucleus (CN), and hippocampus (HP) of uninfected animals. Dotted lines indicate location of marker genes associated with neurons (MAP2), astrocytes (GFAP), microglia (P2RY12), and oligodendrocytes (MOG) within ranked distribution. Fig. S2. T-stochastic neighborhood embedding analysis...

Additional file 3 of Metabolic profiling and pharmacokinetic studies of Baihu-Guizhi decoction in rats by UFLC-Q-TOF–MS/MS and UHPLC-Q-TRAP-MS/MS

Yan He, Zhenkun Zhou, Weijie Li, Yanqiong Zhang, Ruoyao Shi, Tao Li, Linlin Jin, Hongliang Yao, Na Lin & Hao Wu
Additional file 3: Figure S3. The chromatogram of selectivity of the analytes in rat serum.

Additional file 2 of The impact of medically tailored meals and nutrition therapy on biometric and dietary outcomes among food-insecure patients with congestive heart failure: a matched cohort study

Lauren Belak, Caroline Owens, Margaret Smith, Eric Calloway, Laura Samnadda, Heartley Egwuogu & Stacie Schmidt
Additional file 2: SupplementalTable 2. Demographics for individuals with pre- and post- blood pressure data.

Prenatal lead (Pb) exposure is associated with differential placental DNA methylation and hydroxymethylation in a human population

Pei Wen Tung, Elizabeth M. Kennedy, Amber Burt, Karen Hermetz, Margaret Karagas & Carmen J. Marsit
Prenatal lead (Pb) exposure is associated with adverse developmental outcomes and to epigenetic alterations such as DNA methylation and hydroxymethylation in animal models and in newborn blood. Given the importance of the placenta in foetal development, we sought to examine how prenatal Pb exposure was associated with differential placental DNA methylation and hydroxymethylation and to identify affected biological pathways linked to developmental outcomes. Maternal (n = 167) and infant (n = 172) toenail and placenta...

Additional file 2 of 3D-printed hemipelvic prosthesis combined with a dual mobility bearing in patients with primary malignant neoplasm involving the acetabulum: clinical outcomes and finite element analysis

Miao Wang, Tianze Liu, Changli Xu, Chang Liu, Bo Li, Qiujian Lian, Tongjiang Chen, Suchi Qiao & Zhiwei Wang
Additional file 2: Figure S2. The Flow chart shows our rehabilitation training plan.

Supplementary document for Broadband behavior of quadratic metalenses with a wide field of view - 6011078.pdf

Yang Liu, Jianhao Zhang, Xavier Le Roux, Eric Cassan, Delphine Marris-Morini, Laurent Vivien, Carlos Alonso Ramos & Daniele Melati
Supplemental document with additional data, simulation results, and figures.

A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus

Jianyu Zeng, Wenlong Wang, Lei Zhou, Xinna Ge, Jun Han, Xin Guo, Yanhong Chen, Yongning Zhang & Hanchun Yang
Porcine deltacoronavirus (PDCoV) is an emerging enteropathogen causing severe diarrhoea, dehydration, and death in nursing piglets and enormous economic losses for the global swine industry. Furthermore, it can infect multiple animal species including humans. Therefore, a rapid, definitive diagnostic assay is required for the effective control of this zoonotic pathogen. To identify PDCoV, we developed a nucleic acid detection assay combining reverse transcription recombinase-aided amplification (RT-RAA) with a lateral flow dipstick (LFD) targeting the highly...

Additional file 3 of Confronting implicit bias toward patients: a scoping review of post-graduate physician curricula

S. T. Gleicher, M. A. Chalmiers, B. Aiyanyor, R. Jain, N. Kotha, K. Scott, R. S. Song, J. Tram, C. L. Vuong & J. Kesselheim
Additional file 3: Appendix 3. Summary of all 90 studies included in the scoping review (February-November 2020) of post-graduate physician implicit bias curricula [52–119].

Shifting gears to differentiation agents in acute promyelocytic leukemia with resource constraints—a cohort study

Tejasvini Vaid, Mukul Aggarwal, Jasmita Dass, Rishi Dhawan, Pradeep Kumar, Ganesh Kumar Viswanathan, Seema Tyagi, Tulika Seth & Manoranjan Mahapatra
Treatment of acute promyelocytic leukaemia has emerged as a major success in hemato-oncology. While literature from the developed world boasts of outstanding outcomes, there is a paucity of data from the developing world. This study aimed to assess complications and outcomes of acute promyelocytic leukaemia in a resource-constrained setting. We retrospectively collected data from patients diagnosed with APL from January 2016 to December 2020. Sixty-four patients were treated-32 in both the Sanz high and low-risk...

Novel hybrid molecules based on disulfides and 1,2,4-triazole as antiproliferative agents

Junjie Li, Yang Liu, Yanle Men, Zijian Li, Yanping Shi, Xuguang Liu & Baoquan Chen
A series of novel hybrid molecules based on disulfides and 1,2,4-triazole were synthesized, and the structures of the resulting compounds (7a–7g, 8a–8g and 9a–9g) were confirmed by their IR, 1H NMR, 13C NMR and HR-ESI-MS spectroscopic data. The in vitro antiproliferative activity of the tested compounds on human cancer cells A549, Hela, SMMC-7721, and normal cells L929 was determined by CCK-8 assay. The obtained data revealed that most of the tested compounds showed some degrees...

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