Data from: Disentangling the pathways of land use impacts on the functional structure of fish assemblages in Amazon streamsRafael P. Leitão, Jansen Zuanon, David Mouillot, Cecília G. Leal, Robert M. Hughes, Philip R. Kaufmann, Sébastien Villéger, Paulo S. Pompeu, Daniele Kasper, Felipe R. De Paula, Silvio F. B. Ferraz & Toby A. Gardner
Agricultural land use is a primary driver of environmental impacts on streams. However, the causal processes that shape these impacts operate through multiple pathways and at several spatial scales. This complexity undermines the development of more effective management approaches, and illustrates the need for more in-depth studies to assess the mechanisms that determine changes in stream biodiversity. Here we present results of the most comprehensive multi-scale assessment of the biological condition of streams in the...
Data from: Demodifying RNA for transcriptomic analyses of archival formalin-fixed paraffin-embedded samplesLeah C. Wehmas, Charles E. Wood, Remi Gagne, Andrew Williams, Carole Yauk, Mark M. Gosink, Deidre Dalmas, Ruixin Hao, Raegan O'Lone & Susan Hester
Archival formalin-fixed paraffin-embedded (FFPE) tissue samples offer a vast but largely untapped resource for genomic research. The primary technical issues limiting use of FFPE samples are RNA yield and quality. In this study, we evaluated methods to demodify RNA highly fragmented and crosslinked by formalin fixation. Primary endpoints were RNA recovery, RNA-sequencing quality metrics, and transcriptional responses to a reference chemical (phenobarbital, PB). Frozen mouse liver samples from control and PB groups (n=6/group) were divided...
Data from: Is environmental legislation conserving tropical stream faunas? a large-scale assessment of local, riparian and catchment-scale influences on Amazonian stream fishCecília G. Leal, Jos Barlow, Toby Gardner, Robert M. Hughes, Rafael P. Leitão, Ralph Mac Nally, Philip R. Kaufmann, Silvio F. B. Ferraz, Jansen Zuanon, Felipe R. De Paula, Joice Ferreira, James R. Thomson, Gareth D. Lennox, Eurizângela P. Dary, Cristhiana P. Röpke, Paulo S. Pompeu & Toby A. Gardner
1.Agricultural expansion and intensification are major threats to tropical biodiversity. In addition to the direct removal of native vegetation, agricultural expansion often elicits other human-induced disturbances, many of which are poorly addressed by existing environmental legislation and conservation programmes. This is particularly true for tropical freshwater systems, where there is considerable uncertainty about whether a legislative focus on protecting riparian vegetation is sufficient to conserve stream fauna. 2.To assess the extent to which stream fish...
Data from: High-throughput H295R steroidogenesis assay: utility as an alternative and a statistical approach to characterize effects on steroidogenesisDerik E. Haggard, Agnes L. Karmaus, Matthew T. Martin, Richard S. Judson, R. Woodrow Setzer & Katie Paul Friedman
The U.S. Environmental Protection Agency Endocrine Disruptor Screening Program and the Organization for Economic Co-operation and Development (OECD) have used the human adrenocarcinoma (H295R) cell-based assay to predict chemical perturbation of androgen and estrogen production. Recently, a high-throughput H295R (HT-H295R) assay was developed as part of the ToxCast program that includes measurement of 11 hormones, including progestagens, glucocorticoids, androgens, and estrogens. To date, 2012 chemicals have been screened at one concentration; of these, 656 chemicals...
Environmental Protection Agency4
Oregon State University2
Federal University of Lavras2
Universidade Federal de Minas Gerais2
University of Sao Paulo2
Stockholm Environment Institute2
International Life Sciences Institute1
Museu Paraense Emílio Goeldi1
Universidade Federal de Mato Grosso1