4 Works

Data from: Disentangling the pathways of land use impacts on the functional structure of fish assemblages in Amazon streams

Rafael P. Leitão, Jansen Zuanon, David Mouillot, Cecília G. Leal, Robert M. Hughes, Philip R. Kaufmann, Sébastien Villéger, Paulo S. Pompeu, Daniele Kasper, Felipe R. De Paula, Silvio F. B. Ferraz & Toby A. Gardner
Agricultural land use is a primary driver of environmental impacts on streams. However, the causal processes that shape these impacts operate through multiple pathways and at several spatial scales. This complexity undermines the development of more effective management approaches, and illustrates the need for more in-depth studies to assess the mechanisms that determine changes in stream biodiversity. Here we present results of the most comprehensive multi-scale assessment of the biological condition of streams in the...

Data from: Demodifying RNA for transcriptomic analyses of archival formalin-fixed paraffin-embedded samples

Leah C. Wehmas, Charles E. Wood, Remi Gagne, Andrew Williams, Carole Yauk, Mark M. Gosink, Deidre Dalmas, Ruixin Hao, Raegan O'Lone & Susan Hester
Archival formalin-fixed paraffin-embedded (FFPE) tissue samples offer a vast but largely untapped resource for genomic research. The primary technical issues limiting use of FFPE samples are RNA yield and quality. In this study, we evaluated methods to demodify RNA highly fragmented and crosslinked by formalin fixation. Primary endpoints were RNA recovery, RNA-sequencing quality metrics, and transcriptional responses to a reference chemical (phenobarbital, PB). Frozen mouse liver samples from control and PB groups (n=6/group) were divided...

Data from: Is environmental legislation conserving tropical stream faunas? a large-scale assessment of local, riparian and catchment-scale influences on Amazonian stream fish

Cecília G. Leal, Jos Barlow, Toby Gardner, Robert M. Hughes, Rafael P. Leitão, Ralph Mac Nally, Philip R. Kaufmann, Silvio F. B. Ferraz, Jansen Zuanon, Felipe R. De Paula, Joice Ferreira, James R. Thomson, Gareth D. Lennox, Eurizângela P. Dary, Cristhiana P. Röpke, Paulo S. Pompeu & Toby A. Gardner
1.Agricultural expansion and intensification are major threats to tropical biodiversity. In addition to the direct removal of native vegetation, agricultural expansion often elicits other human-induced disturbances, many of which are poorly addressed by existing environmental legislation and conservation programmes. This is particularly true for tropical freshwater systems, where there is considerable uncertainty about whether a legislative focus on protecting riparian vegetation is sufficient to conserve stream fauna. 2.To assess the extent to which stream fish...

Data from: High-throughput H295R steroidogenesis assay: utility as an alternative and a statistical approach to characterize effects on steroidogenesis

Derik E. Haggard, Agnes L. Karmaus, Matthew T. Martin, Richard S. Judson, R. Woodrow Setzer & Katie Paul Friedman
The U.S. Environmental Protection Agency Endocrine Disruptor Screening Program and the Organization for Economic Co-operation and Development (OECD) have used the human adrenocarcinoma (H295R) cell-based assay to predict chemical perturbation of androgen and estrogen production. Recently, a high-throughput H295R (HT-H295R) assay was developed as part of the ToxCast program that includes measurement of 11 hormones, including progestagens, glucocorticoids, androgens, and estrogens. To date, 2012 chemicals have been screened at one concentration; of these, 656 chemicals...

Registration Year

  • 2017
    4

Resource Types

  • Dataset
    4

Affiliations

  • Environmental Protection Agency
    4
  • Oregon State University
    2
  • Federal University of Lavras
    2
  • Universidade Federal de Minas Gerais
    2
  • University of Sao Paulo
    2
  • Lancaster University
    2
  • Stockholm Environment Institute
    2
  • International Life Sciences Institute
    1
  • Museu Paraense Emílio Goeldi
    1
  • Universidade Federal de Mato Grosso
    1