Abstract Background Progesterone receptor (PGR) is a master regulator of uterine function through antagonistic and synergistic interplays with oestrogen receptors. PGR action is primarily mediated by activation functions AF1 and AF2, but their physiological significance is unknown. Results We report the first study of AF1 function in mice. The AF1 mutant mice are infertile with impaired implantation and decidualization. This is associated with a delay in the cessation of epithelial proliferation and in the initiation...

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Abstract Background DNA methyltransferase 1 (DNMT1) exerts imperative functions in neuropathic pain (NP). This study explored the action of RNA interference-mediated DNMT1 silencing in NP by regulating microglial M2 polarization. Methods NP rat models were established using chronic constriction injury (CCI) and highly aggressive proliferating immortalized (HAPI) microglia were treated with lipopolysaccharide (LPS) to induce microglia M1 polarization, followed by treatment of DNMT1 siRNA or si-DNMT1/oe-DNMT1, respectively. The pain threshold of CCI rats was assessed...

Two new complexes, [Cu(dmp)(2-bdppmapy)](CF3SO3)·CH2Cl2 (1) and [Cu(imidazo[4,5-f])(2-bdppmapy)](SCN) (2) (2-bdppmapy = N,N-bis[(diphenylphosphino)methyl]-2-pyridinamine, dmp = 2,9-dimethyl-(1,10)phenanthroline and imidazo[4,5-f] = 1H-imidazo[4,5f][1,10]phenanthroline), have been synthesized in mixed solvents of CH3OH and CH2Cl2 (1:1, V/V). On the basis of studying the structure and photophysical properties of the Cu(I) complexes, the effects of different N ligands and counter anions on the luminescence properties of the Cu(I) complexes were characterized by single-crystal X-ray diffraction, fluorescence spectra and terahertz time-domain spectroscopy (THz-TDS). The...

This document describes the chromaticity coordinates of each point in A1B3C3D2 structure chromaticity distribution diagram

This document describes the chromaticity coordinates of each point in the A1B3C3D2 structure full-color distribution diagram

Additional file 1: Fig. S1. Photograph of the common carotid artery and AVF after surgery. A. Right common carotid artery in normal mice. B. This picture shows the anastomosis of common carotid artery with internal jugular vein in AVF after surgery. The yellow arrow points to the sutures. The inflated jugular vein indicates the patency of the AVF. Scale bars = 1 mm. Fig. S2. Notch signaling conducts signals between neighboring cells. There are 5...

This document describes the basic drawing data of Figure 5

Additional file 1: Figure S1. Comparison of indicators in patients with negative bacterial culture beforeand after appropriate antibiotic therapy.

Additional file 2: Figure S2. The daily alteration of the nCD64 index, PCT, and temperature in infectedpatients.

Abstract Background Neutrophil CD64 (nCD64) index has been widely studied as an indication of bacteria-infected diseases, but the exact usage of nCD64 index in monitoring infections remains debated. So this study aims to investigate the functionality of nCD64 index in tracking infections’ progression and evaluating antibiotic therapy. Methods 160 participants (36 healthy controls, 34 culture-negative patients, 56 respiratory tract infected patients, and 34 bloodstream infected patients) were recruited and divided into groups. Data on nCD64...

Additional file 3: Table S1. The distributionof bacteria strains.

This document describes the basic drawing data of Figure 3 (a)

This document describes the chromaticity coordinates of each point in the A1B3C3D2E1 structure full-color distribution diagram

Additional file 1. Questionnaire.

Additional file 1. Questionnaire.

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive weakness and muscular atrophy in the upper or lower limbs, ultimately leading to paralysis and death. Genetic studies have demonstrated that mutation in the gene encoding fused in sarcoma (FUS) is an uncommon cause of ALS. Here, we report a case of a 31-year-old Asian man with ALS with rare onset of dropped-head syndrome. Symptoms, including asymmetric proximal weakness of the upper limbs,...

Additional file 1: Figure S1. Pan-cancer analysis of ACE2 expression in tumor and para-tumor tissues in the TCGA database. Significance was calculated with Student’s t-test. ns-P>0.05, *P<0.05, **P<0.01, ***P<0.001, ****P < 0.0001. Figure S2. Pan-cancer analysis of the correlations between ACE2 and immunological features. (A) Correlations between ACE2 and 122 immunomodulators (chemokines, receptors, MHC and immunostimulators). The color reveals the correlation coefficient. The asterisks reveal statistical differences assessed by Pearson analysis. (B) Correlations between ACE2...

Acinetobacter baumannii is an important nosocomial pathogen. Upon colonizing a host, A. baumannii are subjected to selective pressure by immune defenses as they adapt to the host environment. However, the mechanism of this pathoadaptation is unknown. Here, we established an in vitro system to evolve A. baumannii driven by the continuous selective pressure exerted by epithelial cells, and we used a combination of experimental evolution, phenotypic characterization and multi-omics analysis to address the underlying mechanism....

1. Design of off-axis reflective system for filamentation;2. Parameters of optimized free-form surface

The rhizome of Rohdea chinensis (Baker) N.Tanaka (RRc) is a famous folk medicine for the treatment of carbuncles and pharyngitis. Steroidal saponins (SSs) were considered to be the most abundant active constituents in RRc. However, to date, the in-depth study of SSs is still lacking. This study was aimed to investigate the SSs profiles of RRc extract by HPLC-ESI-QTOF-MS/MS. Analysis was performed on an Agilent poroshell 120 EC-C18 column (2.1 mm × 100 mm, i.d.,...

Abstract Background Birth weight is considered not only to undermine future growth, but also to induce lifelong diseases; the aim of this study is to explore the relationship between birth weight and adult bone mass. Methods We performed multivariable regression analyses to assess the association of birth weight with bone parameters measured by dual-energy X-ray absorptiometry (DXA) and by quantitative ultrasound (QUS), independently. We also implemented a systemic Mendelian randomization (MR) analysis to explore the...