2 Works

Overexpression of an ALS-associated FUS mutation in C. elegans disrupts NMJ morphology and leads to defective neuromuscular transmission

Sebastian Matthias Markert, Michael Peter Skoruppa, Bin Yu, Ben Mulcahy, Mei Zhen, Shangbang Gao, Michael Sendtner & Christian Stigloher
The amyotrophic lateral sclerosis (ALS) neurodegenerative disorder has been associated with multiple genetic lesions, including mutations in the gene for FUS (Fused in Sarcoma), a nuclear-localized RNA/DNA-binding protein. Neuronal expression of the pathological form of FUS proteins in C. elegans results in mislocalization and aggregation of FUS in the cytoplasm, and leads to impairment of motility. However, the mechanisms by which the mutant FUS disrupts neuronal health and function remain unclear. Here we investigated the...

Data from: On the objectivity, reliability, and validity of deep learning enabled bioimage analyses

Dennis Segebarth, Matthias Griebel, Nikolai Stein, Cora R. Von Collenberg, Corinna Martin, Dominik Fiedler, Lucas B. Comeras, Anupam Sah, Victoria Schoeffler, Theresa Lüffe, Alexander Dürr, Rohini Gupta, Manju Sasi, Christina Lillesaar, Maren D. Lange, Ramon O. Tasan, Nicolas Singewald, Hans-Christian Pape, Christoph M. Flath & Robert Blum
Bioimage analysis of fluorescent labels is widely used in the life sciences. Recent advances in deep learning (DL) allow automating time-consuming manual image analysis processes based on annotated training data. However, manual annotation of fluorescent features with a low signal-to-noise ratio is somewhat subjective. Training DL models on subjective annotations may be instable or yield biased models. In turn, these models may be unable to reliably detect biological effects. An analysis pipeline integrating data annotation,...

Registration Year

  • 2020
    2

Resource Types

  • Dataset
    2

Affiliations

  • University of Würzburg
    2
  • University Hospital Würzburg
    2
  • Huazhong University of Science and Technology
    1
  • University of Münster
    1
  • Lunenfeld-Tanenbaum Research Institute
    1
  • Innsbruck Medical University
    1
  • University of Innsbruck
    1