14 Works

miR-300 in the imprinted DLK1-DIO3 domain suppresses the migration of bladder cancer by regulating the SP1/MMP9 pathway

Huaqing Yan, Jiangfeng Li, Yufan Ying, Haiyun Xie, Hong Chen, Xin Xu & Xiangyi Zheng
Emerging research has suggested that miRNAs play a significant role in oncogenesis and tumor progression by regulating multiple molecular pathways. Here, we investigated miR-300, which inhibited bladder cancer (BCa) migration by regulating the SP1/MMP9 pathway. miR-300, belonging to the DLK1-DIO3 miRNA cluster, is frequently expressed at lower levels in BCa tissue than in adjacent normal tissue due to DNA methylation. Reinforced expression of miR-300 significantly suppressed the migration of BCa cells. We carried out a...

Do the children born after assisted reproductive technology have an increased risk of birth defects? A systematic review and meta-analysis

Jing Zhao, Yi Yan, Xi Huang & Yanping Li
Background: Assisted reproductive technologies (ARTs) have made great progress. However, whether tube baby born after ART were at an increased risk of birth defects is not clear. Objective: To assess whether the ART increases the risk of birth defects in children born after ART. Search strategy: Medline, Google Scholar, and the Cochrane Library were searched. Selection criteria: Clinical trials that evaluate the risk of birth defect in children born after in vitro fertilization (IVF)/intracytoplasmic sperm...

Oxidative stress and NF-κB signaling are involved in LPS induced pulmonary dysplasia in chick embryos

Yun Long, Guang Wang, Ke Li, Zongyi Zhang, Ping Zhang, Jing Zhang, Xiaotan Zhang, Yongping Bao, Xuesong Yang & Pengcheng Wang
Inflammation or dysbacteriosis-derived lipopolysaccharides (LPS) adversely influence the embryonic development of respiratory system. However, the precise pathological mechanisms still remain to be elucidated. In this study, we demonstrated that LPS exposure caused lung maldevelopment in chick embryos, including higher embryo mortality, increased thickness of alveolar gas exchange zone, and accumulation of PAS+ immature pulmonary cells, accompanied with reduced expression of alveolar epithelial cell markers and lamellar body count. Upon LPS exposure, pulmonary cell proliferation was...

Whole-Genome-Sequencing characterization of bloodstream infection-causing hypervirulent Klebsiella pneumoniae of capsular serotype K2 and ST374

Xiaoli Wang, Yingzhou Xie, Gang Li, Jialin Liu, Xiaobin Li, Lijun Tian, Jingyong Sun, Hong-Yu Ou & Hongping Qu
Hypervirulent K. pneumoniae variants (hvKP) have been increasingly reported worldwide, causing metastasis of severe infections such as liver abscesses and bacteremia. The capsular serotype K2 hvKP strains show diverse multi-locus sequence types (MLSTs), but with limited genetics and virulence information. In this study, we report a hypermucoviscous K. pneumoniae strain, RJF293, isolated from a human bloodstream sample in a Chinese hospital. It caused a metastatic infection and fatal septic shock in a critical patient. The...

Whole-Genome-Sequencing characterization of bloodstream infection-causing hypervirulent Klebsiella pneumoniae of capsular serotype K2 and ST374

Xiaoli Wang, Yingzhou Xie, Gang Li, Jialin Liu, Xiaobin Li, Lijun Tian, Jingyong Sun, Hong-Yu Ou & Hongping Qu
Hypervirulent K. pneumoniae variants (hvKP) have been increasingly reported worldwide, causing metastasis of severe infections such as liver abscesses and bacteremia. The capsular serotype K2 hvKP strains show diverse multi-locus sequence types (MLSTs), but with limited genetics and virulence information. In this study, we report a hypermucoviscous K. pneumoniae strain, RJF293, isolated from a human bloodstream sample in a Chinese hospital. It caused a metastatic infection and fatal septic shock in a critical patient. The...

MIR-300 in the imprinted DLK1-DIO3 domain suppresses the migration of bladder cancer by regulating the SP1/MMP9 pathway

Huaqing Yan, Jiangfeng Li, Yufan Ying, Haiyun Xie, Hong Chen, Xin Xu & Xiangyi Zheng
Emerging research has suggested that miRNAs play a significant role in oncogenesis and tumor progression by regulating multiple molecular pathways. Here, we investigated miR-300, which inhibited bladder cancer (BCa) migration by regulating the SP1/MMP9 pathway. miR-300, belonging to the DLK1-DIO3 miRNA cluster, is frequently expressed at lower levels in BCa tissue than in adjacent normal tissue due to DNA methylation. Reinforced expression of miR-300 significantly suppressed the migration of BCa cells. We carried out a...

Oxidative Stress and NF-κB signaling are involved in LPS induced pulmonary dysplasia in chick embryos

Yun Long, Guang Wang, Ke Li, Zong-yi Zhang, Ping Zhang, Jing Zhang, Xiao-tan Zhang, Yongping Bao, Xuesong Yang & Peng-cheng Wang
Inflammation or dysbacteriosis-derived lipopolysaccharides (LPS) adversely influence the embryonic development of respiratory system. However, the precise pathological mechanisms still remain to be elucidated. In this study, we demonstrated that LPS exposure caused lung maldevelopment in chick embryos, including higher embryo mortality, increased thickness of alveolar gas exchange zone, and accumulation of PAS+ immature pulmonary cells, accompanied with reduced expression of alveolar epithelial cell markers and lamellar body count. Upon LPS exposure, pulmonary cell proliferation was...

Do the children born after assisted reproductive technology have an increased risk of birth defects? A systematic review and meta-analysis

Jing Zhao, Yi Yan, Xi Huang & Yanping Li
Background: Assisted reproductive technologies (ARTs) have made great progress. However, whether tube baby born after ART were at an increased risk of birth defects is not clear. Objective: To assess whether the ART increases the risk of birth defects in children born after ART. Search strategy: Medline, Google Scholar, and the Cochrane Library were searched. Selection criteria: Clinical trials that evaluate the risk of birth defect in children born after in vitro fertilization (IVF)/intracytoplasmic sperm...

Oxidative stress and NF-κB signaling are involved in LPS induced pulmonary dysplasia in chick embryos

Yun Long, Guang Wang, Ke Li, Zongyi Zhang, Ping Zhang, Jing Zhang, Xiaotan Zhang, Yongping Bao, Xuesong Yang & Pengcheng Wang
Inflammation or dysbacteriosis-derived lipopolysaccharides (LPS) adversely influence the embryonic development of respiratory system. However, the precise pathological mechanisms still remain to be elucidated. In this study, we demonstrated that LPS exposure caused lung maldevelopment in chick embryos, including higher embryo mortality, increased thickness of alveolar gas exchange zone, and accumulation of PAS+ immature pulmonary cells, accompanied with reduced expression of alveolar epithelial cell markers and lamellar body count. Upon LPS exposure, pulmonary cell proliferation was...

Overexpression of Fbxo6 inactivates spindle checkpoint by interacting with Mad2 and BubR1

Han-Zhang Xu, Zhuo-Qun Wang, Hui-Zhuang Shan, Li Zhou, Li Yang, Hu Lei, Bin Liu & Ying-Li Wu
The spindle assembly checkpoint prevents chromosome mis-segregation during mitosis by delaying sister chromatid separation. Several F-box protein members play critical roles in maintaining genome stability and regulating cell cycle progress via ubiquitin-mediated protein degradation. Here, we showed that Fbxo6 critically regulated spindle checkpoint and chromosome segregation. Fbxo6 was phosphorylated during mitosis. Overexpression of Fbxo6 lead to faster exit from nocodazole-induced mitosis arrest through premature sister chromatid separation. Moreover, we found substantially more binuclear and multilobed...

MIR-300 in the imprinted DLK1-DIO3 domain suppresses the migration of bladder cancer by regulating the SP1/MMP9 pathway

Huaqing Yan, Jiangfeng Li, Yufan Ying, Haiyun Xie, Hong Chen, Xin Xu & Xiangyi Zheng
Emerging research has suggested that miRNAs play a significant role in oncogenesis and tumor progression by regulating multiple molecular pathways. Here, we investigated miR-300, which inhibited bladder cancer (BCa) migration by regulating the SP1/MMP9 pathway. miR-300, belonging to the DLK1-DIO3 miRNA cluster, is frequently expressed at lower levels in BCa tissue than in adjacent normal tissue due to DNA methylation. Reinforced expression of miR-300 significantly suppressed the migration of BCa cells. We carried out a...

Overexpression of Fbxo6 inactivates spindle checkpoint by interacting with Mad2 and BubR1

Han-Zhang Xu, Zhuo-Qun Wang, Hui-Zhuang Shan, Li Zhou, Li Yang, Hu Lei, Bin Liu & Ying-Li Wu
The spindle assembly checkpoint prevents chromosome mis-segregation during mitosis by delaying sister chromatid separation. Several F-box protein members play critical roles in maintaining genome stability and regulating cell cycle progress via ubiquitin-mediated protein degradation. Here, we showed that Fbxo6 critically regulated spindle checkpoint and chromosome segregation. Fbxo6 was phosphorylated during mitosis. Overexpression of Fbxo6 lead to faster exit from nocodazole-induced mitosis arrest through premature sister chromatid separation. Moreover, we found substantially more binuclear and multilobed...

Time-course analysis of nuclear events during conjugation in the marine ciliate Euplotes vannus and comparison with other ciliates (Protozoa, Ciliophora)

Yaohan Jiang, Tengteng Zhang, Adriana Vallesi, Xianyu Yang & Feng Gao
Ciliates represent a morphologically and genetically distinct group of single-celled eukaryotes that segregate germline and somatic functions into two types of nuclei and exhibit complex cytogenetic events during the sexual process of conjugation, which is under the control of the so-called “mating type systems”. Studying conjugation in ciliates may provide insight into our understanding of the origins and evolution of sex and fertilization. In the present work, we studied in detail the sexual process of...

Time-course analysis of nuclear events during conjugation in the marine ciliate Euplotes vannus and comparison with other ciliates (Protozoa, Ciliophora)

Yaohan Jiang, Tengteng Zhang, Adriana Vallesi, Xianyu Yang & Feng Gao
Ciliates represent a morphologically and genetically distinct group of single-celled eukaryotes that segregate germline and somatic functions into two types of nuclei and exhibit complex cytogenetic events during the sexual process of conjugation, which is under the control of the so-called “mating type systems”. Studying conjugation in ciliates may provide insight into our understanding of the origins and evolution of sex and fertilization. In the present work, we studied in detail the sexual process of...

Registration Year

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Affiliations

  • Tongji University
    14
  • Zhejiang University
    12
  • Shanghai Jiao Tong University
    11
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    9
  • Huazhong University of Science and Technology
    8
  • Fudan University
    8
  • Shanghai University of Medicine and Health Sciences
    7
  • Peking University
    6
  • Jinan University
    6
  • Lanzhou Veterinary Research Institute
    5