3 Works

Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife

Maria Razzauti, Maxime Galan, Maria Bernard, Sarah Maman, Christophe Klopp, Nathalie Charbonnel, Muriel Vayssier-Taussat, Marc Eloit & Jean-François Cosson
Background: Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and revealing microbial association patterns within reservoirs. Recently, NGS approaches have been employed to characterize microbial communities of different ecosystems. Yet, their relative efficacy has not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to...

Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus

Emmanuelle Jousselin, Anne-Laure Clamens, Maxime Galan, Maria Bernard, Sarah Maman, Bernhard Gschloessl, Marie-Gabrielle Duport, Andrea S. Meseguer, Federica Calevro, Armelle Coeur D'acier, G. Duport & A.-L. Clamens
The bacterial communities inhabiting arthropods are generally dominated by a few endosymbionts that play an important role in the ecology of their hosts. Rather than comparing bacterial species richness across samples, ecological studies on arthropod endosymbionts often seek to identify the main bacterial strains associated with each specimen studied. The filtering out of contaminants from the results and the accurate taxonomic assignment of sequences are therefore crucial in arthropod microbiome studies. We aimed here to...

Data from: Chronic Trichuris muris Infection Decreases Diversity of the Intestinal Microbiota and Concomitantly Increases the Abundance of Lactobacilli

Jacob Bak Holm, Daniel Sorobetea, Pia Kiilerich, Yuliaxis Ramayo-Caldas, Jordi Estellé, Tao Ma, Lise Madsen, Karsten Kristiansen & Marcus Svensson-Frej
The intestinal microbiota is vital for shaping the local intestinal environment as well as host immunity and metabolism. At the same time, epidemiological and experimental evidence suggest an important role for parasitic worm infections in maintaining the inflammatory and regulatory balance of the immune system. In line with this, the prevalence of persistent worm infections is inversely correlated with the incidence of immune-associated diseases, prompting the use of controlled parasite infections for therapeutic purposes. Despite...

Registration Year

  • 2015
    3

Resource Types

  • Dataset
    3

Affiliations

  • Genetique Animale et Biologie Integrative
    3
  • Montpellier SupAgro
    2
  • Génétique Physiologie et Systèmes d'Elevage
    2
  • Lund University
    1
  • University of Lyon System
    1
  • French National Institute for Agricultural Research
    1
  • University of Copenhagen
    1
  • French Agency for Food, Environmental and Occupational Health & Safety
    1
  • Institut Pasteur
    1
  • Institut National des Sciences Appliquées de Lyon
    1