Data from: A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlifeMaria Razzauti, Maxime Galan, Maria Bernard, Sarah Maman, Christophe Klopp, Nathalie Charbonnel, Muriel Vayssier-Taussat, Marc Eloit & Jean-François Cosson
Background: Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and revealing microbial association patterns within reservoirs. Recently, NGS approaches have been employed to characterize microbial communities of different ecosystems. Yet, their relative efficacy has not been assessed. Here, we compared two NGS approaches, RNA-Sequencing (RNA-Seq) and 16S-metagenomics, assessing their ability to...
Data from: Temperature-sensitive fitness cost of insecticide resistance in Chinese populations of the diamondback moth Plutella xylostellaGang Wu, Denis Bourguet, Lin Jie Zhang, Yu Pu Jing, Xiao Hui Li & Chang Wei Li
Alleles conferring a higher adaptive value in one environment may have a detrimental impact on fitness in another environment. Alleles conferring resistance to pesticides and drugs provide textbook examples of this trade-off as, in addition to conferring resistance to these molecules, they frequently decrease fitness in pesticide/drug-free environments. We show here that resistance to chlorpyrifos, an organophosphate (OP), in Chinese populations of the diamondback moth, Plutella xylostella, is conferred by two mutations of ace1 –...
Data from: Impact of past climatic changes and resource availability on the population demography of three food-specialist beesSimon Dellicour, Denis Michez, Jean-Yves Rasplus & Patrick Mardulyn
Past climate change is known to have strongly impacted current patterns of genetic variation of animals and plants in Europe. However, ecological factors also have the potential to influence demographic history, and thus patterns of genetic variation. In this study, we investigated the impact of past climate, and also the potential impact of host plant species abundance, on intraspecific genetic variation in three co-distributed and related specialized solitary bees of the genus Melitta with very...
Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genusEmmanuelle Jousselin, Anne-Laure Clamens, Maxime Galan, Maria Bernard, Sarah Maman, Bernhard Gschloessl, Marie-Gabrielle Duport, Andrea S. Meseguer, Federica Calevro, Armelle Coeur D'acier, G. Duport & A.-L. Clamens
The bacterial communities inhabiting arthropods are generally dominated by a few endosymbionts that play an important role in the ecology of their hosts. Rather than comparing bacterial species richness across samples, ecological studies on arthropod endosymbionts often seek to identify the main bacterial strains associated with each specimen studied. The filtering out of contaminants from the results and the accurate taxonomic assignment of sequences are therefore crucial in arthropod microbiome studies. We aimed here to...
Data from: Habitat fragmentation alters the properties of a host-parasite network: rodents and their helminths in South-East AsiaFrédéric Bordes, Serge Morand, Shai Pilosof, Julien Claude, Jean-François Cosson, Yannick Chaval, Alexis Ribas, Kittipong Chaisiri, Kim Blasdell, Annelise Tran, Stéphane Dupuy & Boris R. Krasnov
1. While the effects of deforestation and habitat fragmentation on parasite prevalence or richness are well investigated, host–parasite networks are still understudied despite their importance in understanding the mechanisms of these major disturbances. Because fragmentation may negatively impact species occupancy, abundance and co-occurrence, we predict a link between spatiotemporal changes in habitat and the architecture of host–parasite networks. 2. For this, we used an extensive data set on 16 rodent species and 29 helminth species...
Data from: Immunogenetic heterogeneity in a widespread ungulate: the European roe deer (Capreolus capreolus)Erwan Quéméré, Maxime Galan, Jean-François Cosson, François Klein, Stéphane Aulagnier, Emmanuelle Gilot-Fromont, Joël Merlet, A. J. Mark Hewison & Nathalie Charbonnel
Understanding how immune genetic variation is shaped by selective and neutral processes in wild populations is of prime importance in both evolutionary biology and epidemiology. The European roe deer (Capreolus capreolus) has considerably expanded its distribution range these last decades, notably by colonizing agricultural landscapes. This range shift is likely to have led to bottlenecks and increased roe deer exposure to a new range of pathogens that until recently predominantly infected humans and domestic fauna....
University of Lyon System2
Genetique Animale et Biologie Integrative2
Génétique Physiologie et Systèmes d'Elevage2
Université Libre de Bruxelles1
French National Institute for Agricultural Research1
Australian Animal Health Laboratory1
University of Mons1
Comportement et Ecologie de la Faune Sauvage1