45 Works

Data from: Video education versus face-to-face education on inhaler technique for patients with well-controlled or partly-controlled asthma: a phase IV, open-label, non-inferiority, multicenter, randomized, controlled trial

Hye Jung Park, Min Kwang Byun, Jae-Woo Kwon, Woo Kyung Kim, Dong-Ho Nahm, Myung-Goo Lee, Sang-Pyo Lee, Sook Young Lee, Ji-Hyun Lee, Yi Yeong Jeong, You Sook Cho, Jeong-Hee Choi & Byoung Whui Choi
Background: Education on inhaler technique is critical for effective asthma treatment. However, traditionally used face-to-face education is a time-consuming, costly, and often laborious. The current study evaluated the efficacy of a newly developed video-based inhaler technique education method. Methods: A total of 184 subjects with well-controlled or partly-controlled asthma were enrolled from 12 hospitals in South Korea from 30 November 2015 to 01 June 2016. Subjects were randomly divided into two groups in a 1:1...

Additional file 4 of Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination

Mihyun Hwang, Carine Savarin, Jihye Kim, Jennifer Powers, Natasha Towne, Hyunsuk Oh & Cornelia C. Bergmann
Additional file 4: Figure S4. Flow cytometry gating strategy for microglia and BMDM purification from SCs. Doublets are excluded by gating on single cells. Staining with Live/Dead cell marker excluded dead cells from the leukocytes gate. Microglia were gated on CD45intCD11b+ cells and BMDM were gated on CD45hiCD11b+ Ly6G− cells to exclude Ly6G+ neutrophils.

Additional file 3 of Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination

Mihyun Hwang, Carine Savarin, Jihye Kim, Jennifer Powers, Natasha Towne, Hyunsuk Oh & Cornelia C. Bergmann
Additional file 3: Figure S3. Astrocytes do not compensate for impaired uptake of myelin debris by microglia and BMDM. (A) Representative images of GFAP (green), dMBP (red) and DAPI (Blue) in the demyelinated lesion of WT and Trem2−/− mice at days 14 and 20 pi. (B) High magnification image from Trem2−/− mice (white box in A) revealed no overlapped staining between GFAP and dMBP. Scale bar = 20 μm

Supplementary document for High-fidelity optical diffraction tomography of live organisms using non-toxic tunable refractive index media - 6121154.pdf

Dohyeon Lee, Moosung Lee, Haechan KWAK, Young Seo Kim, Jaehyu Shim, Jik Han Jung, Wei Sun Park, Jiho Park, Sumin Lee & YongKeun Park
Supplemental Document

Supplementary document for High-fidelity optical diffraction tomography of live organisms using non-toxic tunable refractive index media - 6121154.pdf

Dohyeon Lee, Moosung Lee, Haechan KWAK, Young Seo Kim, Jaehyu Shim, Jik Han Jung, Wei Sun Park, Jiho Park, Sumin Lee & YongKeun Park
Supplemental Document

Ohmyungsamycin Promotes M1-like Inflammatory Responses to Enhance Host Defense against Mycobacteroides abscessus Infections

Sang Min Jeon, Young Jae Kim, Thanh Quang Nguyen, Jinsheng Cui, Bui Thi Bich Hanh, Prashanta Silwal, Jin Kyung Kim, Jin-Man Kim, Dong-Chan Oh, Jichan Jang & Eun-Kyeong Jo
Ohmyungsamycin A (OMS) is a newly identified cyclic peptide that exerts antimicrobial effects against Mycobacterium tuberculosis. However, its role in nontuberculous mycobacteria (NTMs) infections has not been clarified. Mycobacteroides abscessus (Mabc) is a rapidly growing NTM that has emerged as a human pathogen in both immunocompetent and immunosuppressed individuals. In this study, we demonstrated that OMS had significant antimicrobial effects against Mabc infection in both immunocompetent and immunodeficient mice, and in macrophages. OMS treatment amplified...

Chemical Modulation of SQSTM1/p62-mediated Xenophagy that Targets a Broad Range of Pathogenic Bacteria

Yoon Jee Lee, Jin Kyung Kim, Chan Hoon Jung, Young Jae Kim, Eui Jung Jung, Su Hyun Lee, Ha Rim Choi, Yeon Sung Son, Sang Mi Shim, Sang Min Jeon, Jin Ho Choe, Sang-Hee Lee, Jake Whang, Kyung-Cheol Sohn, Gang Min Hur, Hyun Tae Kim, Jinki Yeom, Eun-Kyeong Jo & Yong Tae Kwon
The N-degron pathway is a proteolytic system in which the N-terminal degrons (N-degrons) of proteins, such as arginine (Nt-Arg), induce the degradation of proteins and subcellular organelles via the ubiquitin-proteasome system (UPS) or macroautophagy/autophagy-lysosome system (hereafter autophagy). Here, we developed the chemical mimics of the N-degron Nt-Arg as a pharmaceutical means to induce targeted degradation of intracellular bacteria via autophagy, such as Salmonella enterica serovar Typhimurium, Escherichia coli, and Streptococcus pyogenes as well as Mycobacterium...

Neurological deterioration in patients with acute ischemic stroke or transient ischemic attack

Tai Hwan Park, Jeong-Kon Lee, Moo-Seok Park, Sang-Soon Park, Keun-Sik Hong, Wi-Sun Ryu, Dong-Eog Kim, Man Seok Park, Kang-Ho Choi, Joon-Tae Kim, Jihoon Kang, Beom Joon Kim, Moon-Ku Han, Jun Lee, Jae-Kwan Cha, Dae-Hyun Kim, Jae Guk Kim, Soo Joo Lee, Yong-Jin Cho, Jee-Hyun Kwon, Dong-Ick Shin, Min-Ju Yeo, Sung Il Sohn, Jeong-Ho Hong, Ji Sung Lee … & Hee-Joon Bae
Objective: To improve epidemiological knowledge of neurological deterioration (ND) in patients with acute ischemic stroke (AIS). Methods: In this prospective observational study, we captured ND prospectively in 29,446 AIS patients admitted to 15 hospitals in Korea within 7 days of stroke onset. ND was defined as an increase in National Institutes of Health Stroke Scale (NIHSS) score ≥2 (total), or ≥1 (motor or consciousness), or any new neurological symptoms. Change of incidence rate after stroke...

LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells

Suyeon Kim, Hanbyoul Cho, Soon-Oh Hong, Se Jin Oh, Hyo-Jung Lee, Eunho Cho, Seon Rang Woo, Joon Seon Song, Joon-Yong Chung, Sung Wook Son, Sang Min Yoon, Yu-Min Jeon, Seunghyun Jeon, Cassian Yee, Kyung-Mi Lee, Stephen M. Hewitt, Jae-Hoon Kim, Kwon-Ho Song & Tae Woo Kim
Immune selection drives tumor cells to acquire refractory phenotypes. We previously demonstrated that cytotoxic T lymphocyte (CTL)-mediated immune pressure enriches NANOG+ tumor cells with stem-like and immune-refractory properties that make them resistant to CTLs. Here, we report that the emergence of refractory phenotypes is highly associated with an aberrant macroautophagic/autophagic state of the NANOG+ tumor cells and that the autophagic phenotype arises through transcriptional induction of MAP1LC3B/LC3B by NANOG. Furthermore, we found that upregulation of...

Additional file 1 of High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Additional file 1: Supplementary Data S1. Clinicopathological features and immunoprofiles of patients with lung adenocarcinoma subjected to flow cytometry. Supplementary Data S2. Clinicopathological features and immune profiles of patients with lung adenocarcinoma subjected to immunohistochemistry. Supplementary Data S3. Clinicopathological features and immune profiles of patients with lung squamous cell carcinoma subjected to immunohistochemistry. Supplementary Data S4. Clinicopathological features and immune profiles of patients with colon adenocarcinoma subjected to immunohistochemistry. Supplementary Data S5. Clinicopathological features and...

Remnant cholesterol and the risk of cardiovascular disease in type 2 diabetes: a nationwide longitudinal cohort study

Ji Hye Huh, Kyung-do Han, Yun Kyung Cho, Eun Roh, Jun Goo Kang, Seong Jin Lee & Sung-Hee Ihm
Abstract Background Elevated remnant cholesterol (remnant-C) is considered a risk factor for cardiovascular disease (CVD); however, whether this notion applies to the East Asian population with type 2 diabetes (T2D) has not been established. This study investigated the association between remnant-C concentrations and the risk of CVD in Korean patients with T2D. Methods By using the Korean National Health Insurance Service database, 1,956,452 patients with T2D and without atherosclerotic CVD who underwent regular health checks...

Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination

Mihyun Hwang, Carine Savarin, Jihye Kim, Jennifer Powers, Natasha Towne, Hyunsuk Oh & Cornelia C. Bergmann
Abstract Background Triggering receptor expressed on myeloid cells 2 (Trem2) plays a protective role in neurodegenerative diseases. By contrast, Trem2 functions can exacerbate tissue damage during respiratory viral or liver infections. We, therefore, investigated the role of Trem2 in a viral encephalomyelitis model associated with prominent Th1 mediated antiviral immunity leading to demyelination. Methods Wild-type (WT) and Trem2 deficient (Trem2−/−) mice were infected with a sublethal glia tropic murine coronavirus (MHV–JHM) intracranially. Disease progression and...

Additional file 2 of Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination

Mihyun Hwang, Carine Savarin, Jihye Kim, Jennifer Powers, Natasha Towne, Hyunsuk Oh & Cornelia C. Bergmann
Additional file 2: Figure S2. Ki67 and active caspase3 positive cells in the SC of naïve WT mice. (A, B) Representative images of Ki67 and active caspase3 positive cells (green) with DAPI (blue) in the ventral funiculus of SC of uninfected mice. Scale bar = 50 μm. (C) Counts of Ki67+ and active caspase3+ cells per mm2. Data show the mean ± SEM from 18 ventral funiculus white matter areas from 3 mice.

Supplementary document for High-fidelity optical diffraction tomography of live organisms using non-toxic tunable refractive index media - 6121154.pdf

Dohyeon Lee, Moosung Lee, Haechan KWAK, Young Seo Kim, Jaehyu Shim, Jik Han Jung, Wei Sun Park, Jiho Park, Sumin Lee & YongKeun Park
Supplemental Document

High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching

Dohyeon Lee, Moosung Lee, Haechan KWAK, Young Seo Kim, Jaehyu Shim, Jik Han Jung, Wei Sun Park, Jiho Park, Sumin Lee & YongKeun Park
Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, the effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization...

Data from: Statin therapy in acute cardioembolic stroke with no guidance-based indication

Hong-Kyun Park, Ji Sung Lee, Keun-Sik Hong, Yong-Jin Cho, Jong-Moo Park, Kyusik Kang, Soo Joo Lee, Jae Guk Kim, Jae-Kwan Cha, Dae-Hyun Kim, Hyun-Wook Nah Nah, Moon-Ku Han, Beom Joon Kim, Tai Hwan Park, Sang-Soon Park, Kyung Bok Lee, Jun Lee, Byung-Chul Lee, Kyung-Ho Yu, Mi Sun Oh, Joon-Tae Kim, Kang-Ho Choi, Dong-Eog Kim, Wi-Sun Ryu, Jay Chol Choi … & Hee-Joon Bae
Objective It is uncertain if patients with cardioembolic stroke and absence of a guidance-based indication for statin therapy should be administered a statin for prevention of subsequent vascular events. This study was performed to determine whether the statin therapy is beneficial to prevent major vascular events in this population. Methods Using a prospective multicenter stroke registry database, we identified acute cardioembolic stroke patients who were hospitalized between 2008 and 2015. Patients who had other established...

LC3B upregulation by NANOG promotes immune resistance and stem-like property through hyperactivation of EGFR signaling in immune-refractory tumor cells

Suyeon Kim, Hanbyoul Cho, Soon-Oh Hong, Se Jin Oh, Hyo-Jung Lee, Eunho Cho, Seon Rang Woo, Joon Seon Song, Joon-Yong Chung, Sung Wook Son, Sang Min Yoon, Yu-Min Jeon, Seunghyun Jeon, Cassian Yee, Kyung-Mi Lee, Stephen M. Hewitt, Jae-Hoon Kim, Kwon-Ho Song & Tae Woo Kim
Immune selection drives tumor cells to acquire refractory phenotypes. We previously demonstrated that cytotoxic T lymphocyte (CTL)-mediated immune pressure enriches NANOG+ tumor cells with stem-like and immune-refractory properties that make them resistant to CTLs. Here, we report that the emergence of refractory phenotypes is highly associated with an aberrant macroautophagic/autophagic state of the NANOG+ tumor cells and that the autophagic phenotype arises through transcriptional induction of MAP1LC3B/LC3B by NANOG. Furthermore, we found that upregulation of...

Additional file 2 of High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Additional file 2: Supplementary Table S1. Clinicopathological features of patients with lung adenocarcinoma subjected to flow cytometry for comprehensive immunoprofiling. Supplementary Table S2. Clinicopathological features of patients with lung adenocarcinoma subjected to immunohistochemistry. Supplementary Table S3. Clinicopathological features of patients with lung squamous cell carcinoma subjected to immunohistochemistry. Supplementary Table S4. Clinicopathological features of patients with colon adenocarcinoma subjected to immunohistochemistry. Supplementary Table S5. Clinicopathological features of non-small cell lung cancer patients with PD-1/PD-L1 blockade...

Additional file 3 of High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Additional file 3: Supplementary Figure S1. Gating strategies for lymphoid and myeloid cells in lung adenocarcinoma. Supplementary Figure S2. Correlation between CD8+ T-cell infiltration and Treg infiltration in human cancer tissues. Supplementary Figure S3. Survival analysis of patients with lung SqCC according to the number of Tregs.

Additional file 3 of High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Additional file 3: Supplementary Figure S1. Gating strategies for lymphoid and myeloid cells in lung adenocarcinoma. Supplementary Figure S2. Correlation between CD8+ T-cell infiltration and Treg infiltration in human cancer tissues. Supplementary Figure S3. Survival analysis of patients with lung SqCC according to the number of Tregs.

Additional file 4 of High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Additional file 4. Supplementary Methods.

High tumor hexokinase-2 expression promotes a pro-tumorigenic immune microenvironment by modulating CD8+/regulatory T-cell infiltration

Sehui Kim, Jaemoon Koh, Seung Geun Song, Jeemin Yim, Miso Kim, Bhumsuk Keam, Young Tae Kim, Jihun Kim, Doo Hyun Chung & Yoon Kyung Jeon
Abstract Background Relationship between cancer cell glycolysis and the landscape of tumor immune microenvironment in human cancers was investigated. Methods Forty-one fresh lung adenocarcinoma (ADC) tissues were analyzed using flow cytometry for comprehensive immunoprofiling. Formalin-fixed tissues were immunostained for hexokinase-2 (HK2) to assess cancer cell glycolysis. For validation, formalin-fixed tissues from 375 lung ADC, 118 lung squamous cell carcinoma (SqCC), 338 colon ADC, and 78 lung cancer patients treated with anti-PD-1/PD-L1 immunotherapy were immunostained for...

Additional file 1 of Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination

Mihyun Hwang, Carine Savarin, Jihye Kim, Jennifer Powers, Natasha Towne, Hyunsuk Oh & Cornelia C. Bergmann
Additional file 1: Figure S1. Tmem119 is downregulated within the MHV–JHM-induced demyelinated lesions. (A) Representative SC images of Tmem119 Ab staining from WT mice at day 20 pi. White dots indicated the demyelinated lesions. Scale bar = 200 μm. (B) High magnification image of co-staining of Tmem119 and IBA1 with DPAI from (A). Arrow depicts Tmem119+IBA1+ cells and asterisk depicts IBA1+ cells but Tmem119− with activated microglia morphology. Scale bar = 50 μm

Chemical modulation of SQSTM1/p62-mediated xenophagy that targets a broad range of pathogenic bacteria

Yoon Jee Lee, Jin Kyung Kim, Chan Hoon Jung, Young Jae Kim, Eui Jung Jung, Su Hyun Lee, Ha Rim Choi, Yeon Sung Son, Sang Mi Shim, Sang Min Jeon, Jin Ho Choe, Sang-Hee Lee, Jake Whang, Kyung-Cheol Sohn, Gang Min Hur, Hyun Tae Kim, Jinki Yeom, Eun-Kyeong Jo & Yong Tae Kwon
The N-degron pathway is a proteolytic system in which the N-terminal degrons (N-degrons) of proteins, such as arginine (Nt-Arg), induce the degradation of proteins and subcellular organelles via the ubiquitin-proteasome system (UPS) or macroautophagy/autophagy-lysosome system (hereafter autophagy). Here, we developed the chemical mimics of the N-degron Nt-Arg as a pharmaceutical means to induce targeted degradation of intracellular bacteria via autophagy, such as Salmonella enterica serovar Typhimurium (S. Typhimurium), Escherichia coli, and Streptococcus pyogenes as well...

Data from: Three-month modified Rankin Scale as a determinant of five-year cumulative costs after ischemic stroke: an analysis of 11,136 patients in Korea

Seong-Eun Kim, Heeyoung Lee, Jun Yup Kim, Keon-Joo Lee, Jihoon Kang, Beom Joon Kim, Moon-Ku Han, Kang-Ho Choi, Joon-Tae Kim, Dong-Ick Shin, Min-Ju Yeo, Jae-Kwan Cha, Dae-Hyun Kim, Hyun-Wook Nah, Dong-Eog Kim, Wi-Sun Ryu, Jong-Moo Park, Kyusik Kang, Jae Guk Kim, Soo Joo Lee, Mi-Sun Oh, Kyung-Ho Yu, Byung-Chul Lee, Hong-Kyun Park, Keun-Sik Hong … & Hee-Joon Bae
Objective: Stroke is a devastating and costly disease; however, there is a paucity of information on long-term costs and on how they differ according to 3-month modified Rankin scale (mRS), which is a primary outcome variable in acute stroke intervention trials. Methods: We analyzed a prospective multicenter stroke registry (Clinical Research Collaboration for Stroke in Korea) database through linkage with claims data from the National Health Insurance Service with follow up to December 2016. Healthcare...

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